Restriction map for
pFN21AE3689
(Linear) (Six-base) MAPSORT of: KIEE3689.seq Check: 8988 from: 1 to: 2055
KIEE3689.orf
With 182 enzymes: SgfI *
March 22, 2012 16:39 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 28 163 899 910 1592 2055
Size: 28 135 736 11 682 463
Fragments arranged by size:
736 682 463 135 28 11
AfeI AGC'GCT
Cuts at: 0 1474 2055
Size: 1474 581
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2033 2055
Size: 2033 22
ApoI r'AATT_y
Cuts at: 0 401 884 913 1878 2055
Size: 401 483 29 965 177
Fragments arranged by size:
965 483 401 177 29
AseI AT'TA_AT
Cuts at: 0 1099 2055
Size: 1099 956
AvaI C'yCGr_G
Cuts at: 0 187 961 2055
Size: 187 774 1094
Fragments arranged by size:
1094 774 187
BamHI G'GATC_C
Cuts at: 0 114 2055
Size: 114 1941
BanII G_rGCy'C
Cuts at: 0 248 2055
Size: 248 1807
BbsI GAAGACnn'nnnn_
Cuts at: 0 151 341 1457 2055
Size: 151 190 1116 598
Fragments arranged by size:
1116 598 190 151
BbvCI CC'TCA_GC
Cuts at: 0 1718 2055
Size: 1718 337
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 263 297 1955 1989 2055
Size: 263 34 1658 34 66
Fragments arranged by size:
1658 263 66 34 34
BclI T'GATC_A
Cuts at: 0 690 2055
Size: 690 1365
BlpI GC'TnA_GC
Cuts at: 0 127 1476 2055
Size: 127 1349 579
Fragments arranged by size:
1349 579 127
BmrI ACTGGGnnnn_n'
Cuts at: 0 417 2055
Size: 417 1638
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1676 2055
Size: 1676 379
Bpu10I CC'TnA_GC
Cuts at: 0 1718 2055
Size: 1718 337
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 244 1580 2055
Size: 244 1336 475
Fragments arranged by size:
1336 475 244
BsaI GGTCTCn'nnnn_
Cuts at: 0 1598 2055
Size: 1598 457
BsaAI yAC'GTr
Cuts at: 0 448 2055
Size: 448 1607
BsaWI w'CCGG_w
Cuts at: 0 117 2055
Size: 117 1938
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 16 2055
Size: 16 2039
BseYI C'CCAG_C
Cuts at: 0 104 500 738 1248 1957 2055
Size: 104 396 238 510 709 98
Fragments arranged by size:
709 510 396 238 104 98
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 737 1831 2055
Size: 737 1094 224
Fragments arranged by size:
1094 737 224
BsiHKAI G_wGCw'C
Cuts at: 0 248 602 2055
Size: 248 354 1453
Fragments arranged by size:
1453 354 248
Bsp1286I G_dGCh'C
Cuts at: 0 248 602 2055
Size: 248 354 1453
Fragments arranged by size:
1453 354 248
BsrFI r'CCGG_y
Cuts at: 0 1985 2055
Size: 1985 70
BstXI CCAn_nnnn'nTGG
Cuts at: 0 108 2055
Size: 108 1947
BstYI r'GATC_y
Cuts at: 0 114 2055
Size: 114 1941
BtgI C'CryG_G
Cuts at: 0 932 2055
Size: 932 1123
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 285 320 2055
Size: 285 35 1735
Fragments arranged by size:
1735 285 35
DraI TTT'AAA
Cuts at: 0 609 1551 2055
Size: 609 942 504
Fragments arranged by size:
942 609 504
EaeI y'GGCC_r
Cuts at: 0 53 2055
Size: 53 2002
EarI CTCTTCn'nnn_
Cuts at: 0 489 771 1245 1303 1402 1568 2055
Size: 489 282 474 58 99 166 487
Fragments arranged by size:
489 487 474 282 166 99 58
EcoICRI GAG'CTC
Cuts at: 0 246 2055
Size: 246 1809
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 28 163 899 910 1592 1676 2055
Size: 28 135 736 11 682 84 379
Fragments arranged by size:
736 682 379 135 84 28 11
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 580 2055
Size: 580 1475
HaeII r_GCGC'y
Cuts at: 0 127 1476 2055
Size: 127 1349 579
Fragments arranged by size:
1349 579 127
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 488 500 520 532 606 638 1567 1599
Size: 488 12 20 12 74 32 929 32
Cuts at: 1599 2035 2055
Size: 436 20
Fragments arranged by size:
929 488 436 74 32 32 20 20
12 12
HincII GTy'rAC
Cuts at: 0 1826 2055
Size: 1826 229
HindIII A'AGCT_T
Cuts at: 0 10 2055
Size: 10 2045
HpaI GTT'AAC
Cuts at: 0 1826 2055
Size: 1826 229
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1565 2055
Size: 1565 490
MscI TGG'CCA
Cuts at: 0 55 2055
Size: 55 2000
MslI CAynn'nnrTG
Cuts at: 0 320 1712 1945 2055
Size: 320 1392 233 110
Fragments arranged by size:
1392 320 233 110
MspA1I CmG'CkG
Cuts at: 0 108 2055
Size: 108 1947
NcoI C'CATG_G
Cuts at: 0 932 2055
Size: 932 1123
NdeI CA'TA_TG
Cuts at: 0 1782 2016 2055
Size: 1782 234 39
Fragments arranged by size:
1782 234 39
PflMI CCAn_nnn'nTGG
Cuts at: 0 77 111 2055
Size: 77 34 1944
Fragments arranged by size:
1944 77 34
PfoI T'CCnGG_A
Cuts at: 0 203 1692 2055
Size: 203 1489 363
Fragments arranged by size:
1489 363 203
PshAI GACnn'nnGTC
Cuts at: 0 383 2055
Size: 383 1672
PsiI TTA'TAA
Cuts at: 0 653 2000 2055
Size: 653 1347 55
Fragments arranged by size:
1347 653 55
PvuII CAG'CTG
Cuts at: 0 108 2055
Size: 108 1947
SacI G_AGCT'C
Cuts at: 0 248 2055
Size: 248 1807
ScaI AGT'ACT
Cuts at: 0 543 2055
Size: 543 1512
SfcI C'TryA_G
Cuts at: 0 1430 2055
Size: 1430 625
SmlI C'TyrA_G
Cuts at: 0 259 1595 2055
Size: 259 1336 460
Fragments arranged by size:
1336 460 259
SnaBI TAC'GTA
Cuts at: 0 448 2055
Size: 448 1607
SspI AAT'ATT
Cuts at: 0 1744 2055
Size: 1744 311
StyI C'CwwG_G
Cuts at: 0 389 932 1272 2055
Size: 389 543 340 783
Fragments arranged by size:
783 543 389 340
SwaI ATTT'AAAT
Cuts at: 0 1551 2055
Size: 1551 504
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 118 1262 2055
Size: 118 1144 793
Fragments arranged by size:
1144 793 118
TatI w'GTAC_w
Cuts at: 0 541 1264 1852 2055
Size: 541 723 588 203
Fragments arranged by size:
723 588 541 203
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 672 704 2055
Size: 672 32 1351
Fragments arranged by size:
1351 672 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 108 2055
Size: 108 1947
Enzymes that do cut:
AcuI AfeI AlfI ApoI AseI AvaI BamHI BanII
BbsI BbvCI BcgI BclI BlpI BmrI BpmI Bpu10I
BpuEI BsaI BsaAI BsaWI BseRI BseYI BsgI BsiHKAI
Bsp1286I BsrFI BstXI BstYI BtgI CspCI DraI EaeI
EarI EcoICRI Eco57MI FalI HaeII Hin4I HincII HindIII
HpaI MmeI MscI MslI MspA1I NcoI NdeI PflMI
PfoI PshAI PsiI PvuII SacI ScaI SfcI SmlI
SnaBI SspI StyI SwaI TaqII TatI TstI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AgeI
AhdI AleI AloI AlwNI ApaI ApaLI AscI AsiSI
AvrII BaeI BanI BbeI BciVI BfrBI BglI BglII
Bme1580I BmgBI BmtI BplI BsaBI BsaHI BsaXI BsiEI
BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI BsrDI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I
BtgZI BtsI ClaI DraIII DrdI EagI EciI EcoNI
EcoO109I EcoRI EcoRV FseI FspI FspAI KasI KpnI
MfeI MluI NaeI NarI NgoMIV NheI NotI NruI
NsiI NspI PacI PasI PciI PmeI PmlI PpiI
PpuMI PspOMI PspXI PsrI PstI PvuI RsrII SacII
SalI SanDI SapI SbfI SexAI SfiI SfoI SgfI
SgrAI SmaI SpeI SphI SrfI StuI Tth111I XbaI
XhoI XmaI XmnI ZraI