Restriction map for
pFN21AE3688
(Linear) (Six-base) MAPSORT of: KIEE3688.seq Check: 4297 from: 1 to: 1383
KIEE3688.orf
With 182 enzymes: SgfI *
December 27, 2011 16:38 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 452 928 1383
Size: 452 476 455
Fragments arranged by size:
476 455 452
AflIII A'CryG_T
Cuts at: 0 114 1383
Size: 114 1269
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 703 1112 1383
Size: 703 409 271
Fragments arranged by size:
703 409 271
AlwNI CAG_nnn'CTG
Cuts at: 0 162 636 1383
Size: 162 474 747
Fragments arranged by size:
747 474 162
ApaI G_GGCC'C
Cuts at: 0 365 1383
Size: 365 1018
AvaI C'yCGr_G
Cuts at: 0 874 1156 1172 1195 1383
Size: 874 282 16 23 188
Fragments arranged by size:
874 282 188 23 16
BanI G'GyrC_C
Cuts at: 0 961 1323 1383
Size: 961 362 60
Fragments arranged by size:
961 362 60
BanII G_rGCy'C
Cuts at: 0 74 208 365 469 1048 1383
Size: 74 134 157 104 579 335
Fragments arranged by size:
579 335 157 134 104 74
BbeI G_GCGC'C
Cuts at: 0 1327 1383
Size: 1327 56
BbsI GAAGACnn'nnnn_
Cuts at: 0 278 1383
Size: 278 1105
BbvCI CC'TCA_GC
Cuts at: 0 190 1383
Size: 190 1193
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 951 985 1383
Size: 951 34 398
Fragments arranged by size:
951 398 34
BglI GCCn_nnn'nGGC
Cuts at: 0 1025 1383
Size: 1025 358
Bme1580I G_kGCm'C
Cuts at: 0 365 626 1383
Size: 365 261 757
Fragments arranged by size:
757 365 261
BmgBI CAC'GTC
Cuts at: 0 144 1351 1383
Size: 144 1207 32
Fragments arranged by size:
1207 144 32
BmrI ACTGGGnnnn_n'
Cuts at: 0 1215 1326 1383
Size: 1215 111 57
Fragments arranged by size:
1215 111 57
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 230 832 901 1062 1169 1381 1383
Size: 230 602 69 161 107 212 2
Fragments arranged by size:
602 230 212 161 107 69 2
Bpu10I CC'TnA_GC
Cuts at: 0 190 1344 1383
Size: 190 1154 39
Fragments arranged by size:
1154 190 39
BsaAI yAC'GTr
Cuts at: 0 117 1383
Size: 117 1266
BsaHI Gr'CG_yC
Cuts at: 0 1324 1383
Size: 1324 59
BsaWI w'CCGG_w
Cuts at: 0 504 752 1383
Size: 504 248 631
Fragments arranged by size:
631 504 248
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 265 295 379 409 1383
Size: 265 30 84 30 974
Fragments arranged by size:
974 265 84 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 538 733 1261 1383
Size: 538 195 528 122
Fragments arranged by size:
538 528 195 122
BseYI C'CCAG_C
Cuts at: 0 123 134 223 347 619 1293 1383
Size: 123 11 89 124 272 674 90
Fragments arranged by size:
674 272 124 123 90 89 11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 952 1373 1383
Size: 952 421 10
Fragments arranged by size:
952 421 10
BsiHKAI G_wGCw'C
Cuts at: 0 74 644 1339 1383
Size: 74 570 695 44
Fragments arranged by size:
695 570 74 44
BsmI GAATG_Cn'
Cuts at: 0 1284 1383
Size: 1284 99
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1367 1383
Size: 1367 16
Bsp1286I G_dGCh'C
Cuts at: 0 74 208 365 469 626 644 1048 1339
Size: 74 134 157 104 157 18 404 291
Cuts at: 1339 1383
Size: 44
Fragments arranged by size:
404 291 157 157 134 104 74 44
18
BspEI T'CCGG_A
Cuts at: 0 752 1383
Size: 752 631
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 147 581 1383
Size: 147 434 802
Fragments arranged by size:
802 434 147
BsrFI r'CCGG_y
Cuts at: 0 92 331 495 1383
Size: 92 239 164 888
Fragments arranged by size:
888 239 164 92
BstAPI GCAn_nnn'nTGC
Cuts at: 0 162 392 1383
Size: 162 230 991
Fragments arranged by size:
991 230 162
BstXI CCAn_nnnn'nTGG
Cuts at: 0 453 1383
Size: 453 930
BstYI r'GATC_y
Cuts at: 0 78 479 1383
Size: 78 401 904
Fragments arranged by size:
904 401 78
Bsu36I CC'TnA_GG
Cuts at: 0 1273 1383
Size: 1273 110
BtgI C'CryG_G
Cuts at: 0 47 1383
Size: 47 1336
BtsI GCAGTG_nn'
Cuts at: 0 217 258 570 833 1383
Size: 217 41 312 263 550
Fragments arranged by size:
550 312 263 217 41
ClaI AT'CG_AT
Cuts at: 0 803 1383
Size: 803 580
DrdI GACnn_nn'nnGTC
Cuts at: 0 157 822 1383
Size: 157 665 561
Fragments arranged by size:
665 561 157
EarI CTCTTCn'nnn_
Cuts at: 0 1172 1383
Size: 1172 211
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 730 1383
Size: 730 653
EcoICRI GAG'CTC
Cuts at: 0 72 1383
Size: 72 1311
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 230 452 832 901 928 1062 1169 1381
Size: 230 222 380 69 27 134 107 212
Cuts at: 1381 1383
Size: 2
Fragments arranged by size:
380 230 222 212 134 107 69 27
2
EcoO109I rG'GnC_Cy
Cuts at: 0 159 361 362 570 593 1140 1147 1383
Size: 159 202 1 208 23 547 7 236
Fragments arranged by size:
547 236 208 202 159 23 7 1
HaeII r_GCGC'y
Cuts at: 0 1327 1383
Size: 1327 56
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 265 297 464 496 515 547 1087 1119
Size: 265 32 167 32 19 32 540 32
Cuts at: 1119 1383
Size: 264
Fragments arranged by size:
540 265 264 167 32 32 32 32
19
KasI G'GCGC_C
Cuts at: 0 1323 1383
Size: 1323 60
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 308 1383
Size: 308 1075
MslI CAynn'nnrTG
Cuts at: 0 928 1094 1383
Size: 928 166 289
Fragments arranged by size:
928 289 166
MspA1I CmG'CkG
Cuts at: 0 311 699 1383
Size: 311 388 684
Fragments arranged by size:
684 388 311
NaeI GCC'GGC
Cuts at: 0 333 497 1383
Size: 333 164 886
Fragments arranged by size:
886 333 164
NarI GG'CG_CC
Cuts at: 0 1324 1383
Size: 1324 59
NcoI C'CATG_G
Cuts at: 0 47 1383
Size: 47 1336
NgoMIV G'CCGG_C
Cuts at: 0 331 495 1383
Size: 331 164 888
Fragments arranged by size:
888 331 164
PasI CC'CwG_GG
Cuts at: 0 409 1048 1383
Size: 409 639 335
Fragments arranged by size:
639 409 335
PfoI T'CCnGG_A
Cuts at: 0 74 1185 1214 1383
Size: 74 1111 29 169
Fragments arranged by size:
1111 169 74 29
PmlI CAC'GTG
Cuts at: 0 117 1383
Size: 117 1266
PpuMI rG'GwC_Cy
Cuts at: 0 159 570 1147 1383
Size: 159 411 577 236
Fragments arranged by size:
577 411 236 159
PspOMI G'GGCC_C
Cuts at: 0 361 1383
Size: 361 1022
PvuII CAG'CTG
Cuts at: 0 699 1383
Size: 699 684
SacI G_AGCT'C
Cuts at: 0 74 1383
Size: 74 1309
SfoI GGC'GCC
Cuts at: 0 1325 1383
Size: 1325 58
SmaI CCC'GGG
Cuts at: 0 1197 1383
Size: 1197 186
SmlI C'TyrA_G
Cuts at: 0 874 1383
Size: 874 509
StyI C'CwwG_G
Cuts at: 0 47 1383
Size: 47 1336
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1308 1383
Size: 1308 75
XhoI C'TCGA_G
Cuts at: 0 874 1383
Size: 874 509
XmaI C'CCGG_G
Cuts at: 0 1195 1383
Size: 1195 188
Enzymes that do cut:
AcuI AflIII AlfI AlwNI ApaI AvaI BanI BanII
BbeI BbsI BbvCI BcgI BglI Bme1580I BmgBI BmrI
BpmI Bpu10I BsaAI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspMI BsrFI
BstAPI BstXI BstYI Bsu36I BtgI BtsI ClaI DrdI
EarI EciI EcoICRI Eco57MI EcoO109I HaeII Hin4I KasI
MmeI MslI MspA1I NaeI NarI NcoI NgoMIV PasI
PfoI PmlI PpuMI PspOMI PvuII SacI SfoI SmaI
SmlI StyI TaqII XhoI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AloI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BciVI BclI BfrBI BglII BlpI
BmtI BplI BpuEI BsaI BsaBI BsiEI BsiWI BspHI
BsrBI BsrDI BsrGI BssHII BssSI BstBI BstEII BstZ17I
BtgZI CspCI DraI DraIII EaeI EagI EcoNI EcoRI
EcoRV FalI FseI FspI FspAI HincII HindIII HpaI
KpnI MfeI MluI MscI NdeI NheI NotI NruI
NsiI NspI PacI PciI PflMI PmeI PpiI PshAI
PsiI PspXI PsrI PstI PvuI RsrII SacII SalI
SanDI SapI SbfI ScaI SexAI SfcI SfiI SgfI
SgrAI SnaBI SpeI SphI SrfI SspI StuI SwaI
TatI TstI Tth111I XbaI XcmI XmnI ZraI