Restriction map for
pFN21AE3687
(Linear) (Six-base) MAPSORT of: KIEE3687.seq Check: 7627 from: 1 to: 1455
KIEE3687.orf
With 182 enzymes: SgfI *
December 27, 2011 16:38 ..
AatII G_ACGT'C
Cuts at: 0 193 1455
Size: 193 1262
AccI GT'mk_AC
Cuts at: 0 1149 1455
Size: 1149 306
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 425 694 1301 1455
Size: 425 269 607 154
Fragments arranged by size:
607 425 269 154
AflII C'TTAA_G
Cuts at: 0 1083 1455
Size: 1083 372
AleI CACnn'nnGTG
Cuts at: 0 935 1455
Size: 935 520
AlwNI CAG_nnn'CTG
Cuts at: 0 125 1455
Size: 125 1330
ApoI r'AATT_y
Cuts at: 0 98 208 275 530 1196 1266 1340 1455
Size: 98 110 67 255 666 70 74 115
Fragments arranged by size:
666 255 115 110 98 74 70 67
AseI AT'TA_AT
Cuts at: 0 1030 1423 1455
Size: 1030 393 32
Fragments arranged by size:
1030 393 32
BamHI G'GATC_C
Cuts at: 0 771 1455
Size: 771 684
BbsI GAAGACnn'nnnn_
Cuts at: 0 37 995 1455
Size: 37 958 460
Fragments arranged by size:
958 460 37
BbvCI CC'TCA_GC
Cuts at: 0 823 1455
Size: 823 632
BciVI GTATCCnnnnn_n'
Cuts at: 0 20 1455
Size: 20 1435
BclI T'GATC_A
Cuts at: 0 108 855 1455
Size: 108 747 600
Fragments arranged by size:
747 600 108
BfrBI ATG'CAT
Cuts at: 0 1021 1455
Size: 1021 434
BglII A'GATC_T
Cuts at: 0 1332 1455
Size: 1332 123
BmrI ACTGGGnnnn_n'
Cuts at: 0 254 1455
Size: 254 1201
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 360 1455
Size: 360 1095
Bpu10I CC'TnA_GC
Cuts at: 0 823 1455
Size: 823 632
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 882 1455
Size: 882 573
BsaHI Gr'CG_yC
Cuts at: 0 190 1455
Size: 190 1265
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 391 885 1455
Size: 391 494 570
Fragments arranged by size:
570 494 391
BseYI C'CCAG_C
Cuts at: 0 566 1455
Size: 566 889
BsiHKAI G_wGCw'C
Cuts at: 0 932 1455
Size: 932 523
BsmI GAATG_Cn'
Cuts at: 0 1359 1455
Size: 1359 96
BsmBI CGTCTCn'nnnn_
Cuts at: 0 181 1455
Size: 181 1274
Bsp1286I G_dGCh'C
Cuts at: 0 932 1455
Size: 932 523
BsrDI GCAATG_nn'
Cuts at: 0 833 1455
Size: 833 622
BsrGI T'GTAC_A
Cuts at: 0 885 1455
Size: 885 570
BstYI r'GATC_y
Cuts at: 0 771 1332 1455
Size: 771 561 123
Fragments arranged by size:
771 561 123
BtsI GCAGTG_nn'
Cuts at: 0 287 398 606 909 1455
Size: 287 111 208 303 546
Fragments arranged by size:
546 303 287 208 111
DraI TTT'AAA
Cuts at: 0 149 1265 1455
Size: 149 1116 190
Fragments arranged by size:
1116 190 149
EarI CTCTTCn'nnn_
Cuts at: 0 329 761 1455
Size: 329 432 694
Fragments arranged by size:
694 432 329
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 425 694 1301 1455
Size: 425 269 607 154
Fragments arranged by size:
607 425 269 154
EcoRI G'AATT_C
Cuts at: 0 275 1455
Size: 275 1180
HincII GTy'rAC
Cuts at: 0 360 1167 1455
Size: 360 807 288
Fragments arranged by size:
807 360 288
HindIII A'AGCT_T
Cuts at: 0 548 1455
Size: 548 907
HpaI GTT'AAC
Cuts at: 0 1167 1455
Size: 1167 288
MfeI C'AATT_G
Cuts at: 0 416 1455
Size: 416 1039
MslI CAynn'nnrTG
Cuts at: 0 851 935 1001 1160 1211 1455
Size: 851 84 66 159 51 244
Fragments arranged by size:
851 244 159 84 66 51
MspA1I CmG'CkG
Cuts at: 0 935 1366 1455
Size: 935 431 89
Fragments arranged by size:
935 431 89
NdeI CA'TA_TG
Cuts at: 0 665 1455
Size: 665 790
NsiI A_TGCA'T
Cuts at: 0 1023 1455
Size: 1023 432
NspI r_CATG'y
Cuts at: 0 436 754 1021 1025 1177 1455
Size: 436 318 267 4 152 278
Fragments arranged by size:
436 318 278 267 152 4
PfoI T'CCnGG_A
Cuts at: 0 923 1455
Size: 923 532
PsiI TTA'TAA
Cuts at: 0 800 1455
Size: 800 655
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 899 931 1455
Size: 899 32 524
Fragments arranged by size:
899 524 32
PvuII CAG'CTG
Cuts at: 0 935 1366 1455
Size: 935 431 89
Fragments arranged by size:
935 431 89
ScaI AGT'ACT
Cuts at: 0 949 1455
Size: 949 506
SexAI A'CCwGG_T
Cuts at: 0 251 1455
Size: 251 1204
SfcI C'TryA_G
Cuts at: 0 299 386 422 1455
Size: 299 87 36 1033
Fragments arranged by size:
1033 299 87 36
SmlI C'TyrA_G
Cuts at: 0 897 1083 1455
Size: 897 186 372
Fragments arranged by size:
897 372 186
SphI G_CATG'C
Cuts at: 0 436 1021 1455
Size: 436 585 434
Fragments arranged by size:
585 436 434
SspI AAT'ATT
Cuts at: 0 154 643 1252 1303 1312 1455
Size: 154 489 609 51 9 143
Fragments arranged by size:
609 489 154 143 51 9
SwaI ATTT'AAAT
Cuts at: 0 1265 1455
Size: 1265 190
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 859 1455
Size: 859 596
TatI w'GTAC_w
Cuts at: 0 267 885 947 1455
Size: 267 618 62 508
Fragments arranged by size:
618 508 267 62
XbaI T'CTAG_A
Cuts at: 0 279 1347 1455
Size: 279 1068 108
Fragments arranged by size:
1068 279 108
XmnI GAAnn'nnTTC
Cuts at: 0 410 1455
Size: 410 1045
ZraI GAC'GTC
Cuts at: 0 191 1455
Size: 191 1264
Enzymes that do cut:
AatII AccI AcuI AflII AleI AlwNI ApoI AseI
BamHI BbsI BbvCI BciVI BclI BfrBI BglII BmrI
BplI Bpu10I BpuEI BsaHI BseRI BseYI BsiHKAI BsmI
BsmBI Bsp1286I BsrDI BsrGI BstYI BtsI DraI EarI
Eco57MI EcoRI HincII HindIII HpaI MfeI MslI MspA1I
NdeI NsiI NspI PfoI PsiI PsrI PvuII ScaI
SexAI SfcI SmlI SphI SspI SwaI TaqII TatI
XbaI XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflIII AgeI AhdI AlfI
AloI ApaI ApaLI AscI AsiSI AvaI AvrII BaeI
BanI BanII BbeI BcgI BglI BlpI Bme1580I BmgBI
BmtI BpmI BsaI BsaAI BsaBI BsaWI BsaXI BsgI
BsiEI BsiWI BspEI BspHI BspMI BsrBI BsrFI BssHII
BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgI
BtgZI ClaI CspCI DraIII DrdI EaeI EagI EciI
EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspI FspAI
HaeII Hin4I KasI KpnI MluI MmeI MscI NaeI
NarI NcoI NgoMIV NheI NotI NruI PacI PasI
PciI PflMI PmeI PmlI PpiI PpuMI PshAI PspOMI
PspXI PstI PvuI RsrII SacI SacII SalI SanDI
SapI SbfI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SrfI StuI StyI TstI Tth111I XcmI XhoI
XmaI