Restriction map for
pFN21AE3681
(Linear) (Six-base) MAPSORT of: KIEE3681.seq Check: 5176 from: 1 to: 1089
KIEE3681.orf
With 182 enzymes: SgfI *
July 3, 2012 18:14 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 975 1089
Size: 975 114
AatII G_ACGT'C
Cuts at: 0 673 1089
Size: 673 416
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 354 1089
Size: 354 735
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 193 225 1089
Size: 193 32 864
Fragments arranged by size:
864 193 32
AlwNI CAG_nnn'CTG
Cuts at: 0 425 744 753 1089
Size: 425 319 9 336
Fragments arranged by size:
425 336 319 9
ApaLI G'TGCA_C
Cuts at: 0 988 1089
Size: 988 101
AvaI C'yCGr_G
Cuts at: 0 223 283 855 925 1089
Size: 223 60 572 70 164
Fragments arranged by size:
572 223 164 70 60
BanI G'GyrC_C
Cuts at: 0 278 861 1089
Size: 278 583 228
Fragments arranged by size:
583 278 228
BanII G_rGCy'C
Cuts at: 0 102 441 1089
Size: 102 339 648
Fragments arranged by size:
648 339 102
BbeI G_GCGC'C
Cuts at: 0 865 1089
Size: 865 224
BbvCI CC'TCA_GC
Cuts at: 0 310 772 1089
Size: 310 462 317
Fragments arranged by size:
462 317 310
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 608 642 1089
Size: 608 34 447
Fragments arranged by size:
608 447 34
BglII A'GATC_T
Cuts at: 0 347 410 1089
Size: 347 63 679
Fragments arranged by size:
679 347 63
Bme1580I G_kGCm'C
Cuts at: 0 396 626 992 1089
Size: 396 230 366 97
Fragments arranged by size:
396 366 230 97
BmgBI CAC'GTC
Cuts at: 0 305 1089
Size: 305 784
BmrI ACTGGGnnnn_n'
Cuts at: 0 435 838 1089
Size: 435 403 251
Fragments arranged by size:
435 403 251
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 195 1089
Size: 195 894
Bpu10I CC'TnA_GC
Cuts at: 0 310 772 1089
Size: 310 462 317
Fragments arranged by size:
462 317 310
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 232 559 1089
Size: 232 327 530
Fragments arranged by size:
530 327 232
BsaAI yAC'GTr
Cuts at: 0 273 1089
Size: 273 816
BsaHI Gr'CG_yC
Cuts at: 0 80 670 862 1089
Size: 80 590 192 227
Fragments arranged by size:
590 227 192 80
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 193 223 1089
Size: 193 30 866
Fragments arranged by size:
866 193 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 168 523 541 1089
Size: 168 355 18 548
Fragments arranged by size:
548 355 168 18
BseYI C'CCAG_C
Cuts at: 0 5 139 1089
Size: 5 134 950
Fragments arranged by size:
950 134 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 934 1089
Size: 934 155
BsiEI CG_ry'CG
Cuts at: 0 405 1089
Size: 405 684
BsiHKAI G_wGCw'C
Cuts at: 0 102 292 441 539 992 1089
Size: 102 190 149 98 453 97
Fragments arranged by size:
453 190 149 102 98 97
Bsp1286I G_dGCh'C
Cuts at: 0 102 292 396 441 539 626 992 1089
Size: 102 190 104 45 98 87 366 97
Fragments arranged by size:
366 190 104 102 98 97 87 45
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 684 975 1089
Size: 684 291 114
Fragments arranged by size:
684 291 114
BsrBI CCG'CTC
Cuts at: 0 930 1050 1089
Size: 930 120 39
Fragments arranged by size:
930 120 39
BsrFI r'CCGG_y
Cuts at: 0 651 995 1089
Size: 651 344 94
Fragments arranged by size:
651 344 94
BssHII G'CGCG_C
Cuts at: 0 660 662 934 1089
Size: 660 2 272 155
Fragments arranged by size:
660 272 155 2
BssSI C'ACGA_G
Cuts at: 0 480 1089
Size: 480 609
BstAPI GCAn_nnn'nTGC
Cuts at: 0 699 1089
Size: 699 390
BstEII G'GTnAC_C
Cuts at: 0 942 1089
Size: 942 147
BstYI r'GATC_y
Cuts at: 0 227 347 410 1089
Size: 227 120 63 679
Fragments arranged by size:
679 227 120 63
BtgI C'CryG_G
Cuts at: 0 65 680 1089
Size: 65 615 409
Fragments arranged by size:
615 409 65
BtsI GCAGTG_nn'
Cuts at: 0 626 990 1089
Size: 626 364 99
Fragments arranged by size:
626 364 99
DraIII CAC_nnn'GTG
Cuts at: 0 846 1089
Size: 846 243
EaeI y'GGCC_r
Cuts at: 0 402 653 1089
Size: 402 251 436
Fragments arranged by size:
436 402 251
EagI C'GGCC_G
Cuts at: 0 402 1089
Size: 402 687
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 772 873 1089
Size: 772 101 216
Fragments arranged by size:
772 216 101
EcoICRI GAG'CTC
Cuts at: 0 100 439 1089
Size: 100 339 650
Fragments arranged by size:
650 339 100
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 195 354 1089
Size: 195 159 735
Fragments arranged by size:
735 195 159
EcoNI CCTnn'n_nnAGG
Cuts at: 0 523 1089
Size: 523 566
EcoO109I rG'GnC_Cy
Cuts at: 0 87 1089
Size: 87 1002
EcoRV GAT'ATC
Cuts at: 0 241 1089
Size: 241 848
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 366 1089
Size: 366 723
HaeII r_GCGC'y
Cuts at: 0 492 865 1089
Size: 492 373 224
Fragments arranged by size:
492 373 224
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 230 262 1089
Size: 230 32 827
Fragments arranged by size:
827 230 32
HincII GTy'rAC
Cuts at: 0 255 643 1089
Size: 255 388 446
Fragments arranged by size:
446 388 255
KasI G'GCGC_C
Cuts at: 0 861 1089
Size: 861 228
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 590 917 1079 1089
Size: 590 327 162 10
Fragments arranged by size:
590 327 162 10
MslI CAynn'nnrTG
Cuts at: 0 790 1089
Size: 790 299
MspA1I CmG'CkG
Cuts at: 0 38 215 326 495 682 690 1089
Size: 38 177 111 169 187 8 399
Fragments arranged by size:
399 187 177 169 111 38 8
NaeI GCC'GGC
Cuts at: 0 653 997 1089
Size: 653 344 92
Fragments arranged by size:
653 344 92
NarI GG'CG_CC
Cuts at: 0 862 1089
Size: 862 227
NcoI C'CATG_G
Cuts at: 0 65 1089
Size: 65 1024
NgoMIV G'CCGG_C
Cuts at: 0 651 995 1089
Size: 651 344 94
Fragments arranged by size:
651 344 94
PflMI CCAn_nnn'nTGG
Cuts at: 0 425 1089
Size: 425 664
PfoI T'CCnGG_A
Cuts at: 0 230 1089
Size: 230 859
PmlI CAC'GTG
Cuts at: 0 273 1089
Size: 273 816
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 193 225 1089
Size: 193 32 864
Fragments arranged by size:
864 193 32
PshAI GACnn'nnGTC
Cuts at: 0 116 1089
Size: 116 973
PspXI vC'TCGA_Gb
Cuts at: 0 283 1089
Size: 283 806
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 313 345 1089
Size: 313 32 744
Fragments arranged by size:
744 313 32
PstI C_TGCA'G
Cuts at: 0 195 695 749 1089
Size: 195 500 54 340
Fragments arranged by size:
500 340 195 54
PvuII CAG'CTG
Cuts at: 0 215 326 495 690 1089
Size: 215 111 169 195 399
Fragments arranged by size:
399 215 195 169 111
SacI G_AGCT'C
Cuts at: 0 102 441 1089
Size: 102 339 648
Fragments arranged by size:
648 339 102
SacII CC_GC'GG
Cuts at: 0 683 1089
Size: 683 406
SfcI C'TryA_G
Cuts at: 0 191 691 745 1089
Size: 191 500 54 344
Fragments arranged by size:
500 344 191 54
SfoI GGC'GCC
Cuts at: 0 863 1089
Size: 863 226
SmlI C'TyrA_G
Cuts at: 0 223 247 283 574 1089
Size: 223 24 36 291 515
Fragments arranged by size:
515 291 223 36 24
StuI AGG'CCT
Cuts at: 0 124 1089
Size: 124 965
StyI C'CwwG_G
Cuts at: 0 65 1089
Size: 65 1024
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 251 417 857 1089
Size: 251 166 440 232
Fragments arranged by size:
440 251 232 166
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 375 407 1004 1036 1089
Size: 375 32 597 32 53
Fragments arranged by size:
597 375 53 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 499 1089
Size: 499 590
XhoI C'TCGA_G
Cuts at: 0 223 283 1089
Size: 223 60 806
Fragments arranged by size:
806 223 60
ZraI GAC'GTC
Cuts at: 0 671 1089
Size: 671 418
Enzymes that do cut:
AarI AatII AcuI AloI AlwNI ApaLI AvaI BanI
BanII BbeI BbvCI BcgI BglII Bme1580I BmgBI BmrI
BpmI Bpu10I BpuEI BsaAI BsaHI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrFI BssHII
BssSI BstAPI BstEII BstYI BtgI BtsI DraIII EaeI
EagI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRV FalI
HaeII Hin4I HincII KasI MmeI MslI MspA1I NaeI
NarI NcoI NgoMIV PflMI PfoI PmlI PpiI PshAI
PspXI PsrI PstI PvuII SacI SacII SfcI SfoI
SmlI StuI StyI TaqII TstI XcmI XhoI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI ApaI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbsI BciVI BclI BfrBI BglI BlpI
BmtI BplI BsaI BsaBI BsaWI BsiWI BsmI BsmBI
BspEI BspHI BsrDI BsrGI BstBI BstXI BstZ17I Bsu36I
BtgZI ClaI CspCI DraI DrdI EarI EcoRI FseI
FspI FspAI HindIII HpaI KpnI MfeI MluI MscI
NdeI NheI NotI NruI NsiI NspI PacI PasI
PciI PmeI PpuMI PsiI PspOMI PvuI RsrII SalI
SanDI SapI SbfI ScaI SexAI SfiI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI SwaI TatI
Tth111I XbaI XmaI XmnI