(Linear) (Six-base) MAPSORT of: KIEE3680.seq  Check: 2005  from: 1  to: 483



KIEE3680.orf

 With 182 enzymes: SgfI * 

                        December 27, 2011 16:38  ..

AccI GT'mk_AC

Cuts at:      0     236     483 
   Size:        236     247
 
AlwNI CAG_nnn'CTG

Cuts at:      0      82     483 
   Size:         82     401
 
BanI G'GyrC_C

Cuts at:      0     466     483 
   Size:        466      17
 
BanII G_rGCy'C

Cuts at:      0     459     483 
   Size:        459      24
 
BbvCI CC'TCA_GC

Cuts at:      0      42     483 
   Size:         42     441
 
Bme1580I G_kGCm'C

Cuts at:      0     469     483 
   Size:        469      14
 
BmgBI CAC'GTC

Cuts at:      0     429     483 
   Size:        429      54
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      41     483 
   Size:         41     442
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      92     483 
   Size:         92     391
 
Bpu10I CC'TnA_GC

Cuts at:      0      42     483 
   Size:         42     441
 
BsaAI yAC'GTr

Cuts at:      0     240     483 
   Size:        240     243
 
BseYI C'CCAG_C

Cuts at:      0     188     372     483 
   Size:        188     184     111

  Fragments arranged by size: 

                 188     184     111
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     247     262     483 
   Size:        247      15     221

  Fragments arranged by size: 

                 247     221      15
 
BsiHKAI G_wGCw'C

Cuts at:      0     339     459     483 
   Size:        339     120      24

  Fragments arranged by size: 

                 339     120      24
 
Bsp1286I G_dGCh'C

Cuts at:      0     339     459     469     483 
   Size:        339     120      10      14

  Fragments arranged by size: 

                 339     120      14      10
 
BsrFI r'CCGG_y

Cuts at:      0     331     483 
   Size:        331     152
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     348     483 
   Size:        348     135
 
EaeI y'GGCC_r

Cuts at:      0      26     483 
   Size:         26     457
 
EarI CTCTTCn'nnn_

Cuts at:      0      65     483 
   Size:         65     418
 
EcoICRI GAG'CTC

Cuts at:      0     457     483 
   Size:        457      26
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      92     483 
   Size:         92     391
 
EcoO109I rG'GnC_Cy

Cuts at:      0      13     148     483 
   Size:         13     135     335

  Fragments arranged by size: 

                 335     135      13
 
HaeII r_GCGC'y

Cuts at:      0     199     483 
   Size:        199     284
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     297     329     483 
   Size:        297      32     154

  Fragments arranged by size: 

                 297     154      32
 
HincII GTy'rAC

Cuts at:      0      87     483 
   Size:         87     396
 
HpaI GTT'AAC

Cuts at:      0      87     483 
   Size:         87     396
 
MspA1I CmG'CkG

Cuts at:      0     318     483 
   Size:        318     165
 
PfoI T'CCnGG_A

Cuts at:      0       9     483 
   Size:          9     474
 
PpuMI rG'GwC_Cy

Cuts at:      0      13     483 
   Size:         13     470
 
PstI C_TGCA'G

Cuts at:      0     425     454     483 
   Size:        425      29      29

  Fragments arranged by size: 

                 425      29      29
 
PvuII CAG'CTG

Cuts at:      0     318     483 
   Size:        318     165
 
SacI G_AGCT'C

Cuts at:      0     459     483 
   Size:        459      24
 
SbfI CC_TGCA'GG

Cuts at:      0     425     483 
   Size:        425      58
 
SfcI C'TryA_G

Cuts at:      0     421     450     483 
   Size:        421      29      33

  Fragments arranged by size: 

                 421      33      29
 
SgrAI Cr'CCGG_yG

Cuts at:      0     331     483 
   Size:        331     152
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      28     258     483 
   Size:         28     230     225

  Fragments arranged by size: 

                 230     225      28
 

 Enzymes that do cut:

     AccI    AlwNI     BanI    BanII    BbvCI Bme1580I    BmgBI     BmrI
     BpmI   Bpu10I    BsaAI    BseYI     BsgI  BsiHKAI Bsp1286I    BsrFI
     DrdI     EaeI     EarI  EcoICRI  Eco57MI EcoO109I    HaeII    Hin4I
   HincII     HpaI   MspA1I     PfoI    PpuMI     PstI    PvuII     SacI
     SbfI     SfcI    SgrAI     XcmI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BbeI
     BbsI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
     BmtI     BplI    BpuEI     BsaI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI
    CspCI     DraI   DraIII     EagI     EciI    EcoNI    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI  HindIII     KasI     KpnI     MfeI
     MluI     MmeI     MscI     MslI     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI    PflMI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PvuI    RsrII    SacII     SalI    SanDI     SapI
     ScaI    SexAI     SfiI     SfoI     SgfI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI    TaqII
     TatI     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI