Restriction map for
pFN21AE3678
(Linear) (Six-base) MAPSORT of: KIEE3678.seq Check: 502 from: 1 to: 951
KIEE3678.orf
With 182 enzymes: SgfI *
December 27, 2011 16:38 ..
Acc65I G'GTAC_C
Cuts at: 0 353 951
Size: 353 598
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 440 587 951
Size: 440 147 364
Fragments arranged by size:
440 364 147
AgeI A'CCGG_T
Cuts at: 0 216 951
Size: 216 735
AleI CACnn'nnGTG
Cuts at: 0 518 951
Size: 518 433
AlwNI CAG_nnn'CTG
Cuts at: 0 711 951
Size: 711 240
ApaI G_GGCC'C
Cuts at: 0 209 951
Size: 209 742
ApaLI G'TGCA_C
Cuts at: 0 435 951
Size: 435 516
AvaI C'yCGr_G
Cuts at: 0 292 477 951
Size: 292 185 474
Fragments arranged by size:
474 292 185
BanI G'GyrC_C
Cuts at: 0 213 313 353 801 951
Size: 213 100 40 448 150
Fragments arranged by size:
448 213 150 100 40
BanII G_rGCy'C
Cuts at: 0 209 484 491 951
Size: 209 275 7 460
Fragments arranged by size:
460 275 209 7
BbeI G_GCGC'C
Cuts at: 0 317 951
Size: 317 634
BbsI GAAGACnn'nnnn_
Cuts at: 0 479 951
Size: 479 472
BglI GCCn_nnn'nGGC
Cuts at: 0 40 204 951
Size: 40 164 747
Fragments arranged by size:
747 164 40
Bme1580I G_kGCm'C
Cuts at: 0 209 439 811 835 951
Size: 209 230 372 24 116
Fragments arranged by size:
372 230 209 116 24
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 721 739 951
Size: 721 18 212
Fragments arranged by size:
721 212 18
BsaAI yAC'GTr
Cuts at: 0 231 951
Size: 231 720
BsaHI Gr'CG_yC
Cuts at: 0 314 951
Size: 314 637
BsaWI w'CCGG_w
Cuts at: 0 216 951
Size: 216 735
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 154 157 655 736 951
Size: 154 3 498 81 215
Fragments arranged by size:
498 215 154 81 3
BseYI C'CCAG_C
Cuts at: 0 309 828 887 951
Size: 309 519 59 64
Fragments arranged by size:
519 309 64 59
BsiEI CG_ry'CG
Cuts at: 0 32 951
Size: 32 919
BsiHKAI G_wGCw'C
Cuts at: 0 439 484 947 951
Size: 439 45 463 4
Fragments arranged by size:
463 439 45 4
Bsp1286I G_dGCh'C
Cuts at: 0 209 439 484 491 811 835 947 951
Size: 209 230 45 7 320 24 112 4
Fragments arranged by size:
320 230 209 112 45 24 7 4
BspHI T'CATG_A
Cuts at: 0 491 951
Size: 491 460
BsrBI CCG'CTC
Cuts at: 0 249 726 951
Size: 249 477 225
Fragments arranged by size:
477 249 225
BsrFI r'CCGG_y
Cuts at: 0 39 147 216 798 951
Size: 39 108 69 582 153
Fragments arranged by size:
582 153 108 69 39
BssHII G'CGCG_C
Cuts at: 0 686 688 951
Size: 686 2 263
Fragments arranged by size:
686 263 2
BssSI C'ACGA_G
Cuts at: 0 136 892 951
Size: 136 756 59
Fragments arranged by size:
756 136 59
BstXI CCAn_nnnn'nTGG
Cuts at: 0 309 951
Size: 309 642
BstYI r'GATC_y
Cuts at: 0 260 279 446 951
Size: 260 19 167 505
Fragments arranged by size:
505 260 167 19
BtgI C'CryG_G
Cuts at: 0 6 951
Size: 6 945
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 545 951
Size: 545 406
BtsI GCAGTG_nn'
Cuts at: 0 793 951
Size: 793 158
EaeI y'GGCC_r
Cuts at: 0 29 951
Size: 29 922
EagI C'GGCC_G
Cuts at: 0 29 951
Size: 29 922
EarI CTCTTCn'nnn_
Cuts at: 0 563 951
Size: 563 388
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 616 951
Size: 616 335
EcoICRI GAG'CTC
Cuts at: 0 482 951
Size: 482 469
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 440 587 721 739 951
Size: 440 147 134 18 212
Fragments arranged by size:
440 212 147 134 18
EcoNI CCTnn'n_nnAGG
Cuts at: 0 361 928 951
Size: 361 567 23
Fragments arranged by size:
567 361 23
EcoO109I rG'GnC_Cy
Cuts at: 0 131 951
Size: 131 820
HaeII r_GCGC'y
Cuts at: 0 317 615 718 742 775 951
Size: 317 298 103 24 33 176
Fragments arranged by size:
317 298 176 103 33 24
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 115 147 466 498 523 555 951
Size: 115 32 319 32 25 32 396
Fragments arranged by size:
396 319 115 32 32 32 25
KasI G'GCGC_C
Cuts at: 0 313 951
Size: 313 638
KpnI G_GTAC'C
Cuts at: 0 357 951
Size: 357 594
MslI CAynn'nnrTG
Cuts at: 0 304 518 951
Size: 304 214 433
Fragments arranged by size:
433 304 214
MspA1I CmG'CkG
Cuts at: 0 351 951
Size: 351 600
NaeI GCC'GGC
Cuts at: 0 41 951
Size: 41 910
NarI GG'CG_CC
Cuts at: 0 314 951
Size: 314 637
NgoMIV G'CCGG_C
Cuts at: 0 39 951
Size: 39 912
NotI GC'GGCC_GC
Cuts at: 0 29 951
Size: 29 922
PfoI T'CCnGG_A
Cuts at: 0 449 951
Size: 449 502
PpuMI rG'GwC_Cy
Cuts at: 0 131 951
Size: 131 820
PspOMI G'GGCC_C
Cuts at: 0 205 951
Size: 205 746
PspXI vC'TCGA_Gb
Cuts at: 0 477 951
Size: 477 474
PstI C_TGCA'G
Cuts at: 0 335 662 746 951
Size: 335 327 84 205
Fragments arranged by size:
335 327 205 84
SacI G_AGCT'C
Cuts at: 0 484 951
Size: 484 467
SanDI GG'GwC_CC
Cuts at: 0 131 951
Size: 131 820
SfcI C'TryA_G
Cuts at: 0 331 658 742 951
Size: 331 327 84 209
Fragments arranged by size:
331 327 209 84
SfoI GGC'GCC
Cuts at: 0 315 951
Size: 315 636
SgrAI Cr'CCGG_yG
Cuts at: 0 39 951
Size: 39 912
SmlI C'TyrA_G
Cuts at: 0 477 951
Size: 477 474
StyI C'CwwG_G
Cuts at: 0 170 209 951
Size: 170 39 742
Fragments arranged by size:
742 170 39
TatI w'GTAC_w
Cuts at: 0 386 951
Size: 386 565
XhoI C'TCGA_G
Cuts at: 0 477 951
Size: 477 474
Enzymes that do cut:
Acc65I AcuI AgeI AleI AlwNI ApaI ApaLI AvaI
BanI BanII BbeI BbsI BglI Bme1580I BpmI BsaAI
BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspHI
BsrBI BsrFI BssHII BssSI BstXI BstYI BtgI BtgZI
BtsI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I HaeII Hin4I KasI KpnI MslI MspA1I NaeI
NarI NgoMIV NotI PfoI PpuMI PspOMI PspXI PstI
SacI SanDI SfcI SfoI SgrAI SmlI StyI TatI
XhoI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AhdI
AlfI AloI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BbvCI BcgI BciVI BclI BfrBI BglII BlpI
BmgBI BmrI BmtI BplI Bpu10I BpuEI BsaI BsaBI
BsaXI BsgI BsiWI BsmI BsmBI BspEI BspMI BsrDI
BsrGI BstAPI BstBI BstEII BstZ17I Bsu36I ClaI CspCI
DraI DraIII DrdI EcoRI EcoRV FalI FseI FspI
FspAI HincII HindIII HpaI MfeI MluI MmeI MscI
NcoI NdeI NheI NruI NsiI NspI PacI PasI
PciI PflMI PmeI PmlI PpiI PshAI PsiI PsrI
PvuI PvuII RsrII SacII SalI SapI SbfI ScaI
SexAI SfiI SgfI SmaI SnaBI SpeI SphI SrfI
SspI StuI SwaI TaqII TstI Tth111I XbaI XcmI
XmaI XmnI ZraI