(Linear) (Six-base) MAPSORT of: KIEE3675.seq  Check: 8499  from: 1  to: 1098



KIEE3675.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:45  ..

AatII G_ACGT'C

Cuts at:      0    1042    1098 
   Size:       1042      56
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     133     391     627    1098 
   Size:        133     258     236     471

  Fragments arranged by size: 

                 471     258     236     133
 
AleI CACnn'nnGTG

Cuts at:      0     949    1098 
   Size:        949     149
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     374    1098 
   Size:        374     724
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1066    1098 
   Size:       1066      32
 
ApoI r'AATT_y

Cuts at:      0     367    1024    1056    1098 
   Size:        367     657      32      42

  Fragments arranged by size: 

                 657     367      42      32
 
BanI G'GyrC_C

Cuts at:      0      37    1098 
   Size:         37    1061
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     816    1098 
   Size:        816     282
 
Bme1580I G_kGCm'C

Cuts at:      0      40    1098 
   Size:         40    1058
 
BmgBI CAC'GTC

Cuts at:      0     233    1098 
   Size:        233     865
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     840    1098 
   Size:        840     258
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     995    1098 
   Size:        995     103
 
BsaHI Gr'CG_yC

Cuts at:      0     284    1039    1098 
   Size:        284     755      59

  Fragments arranged by size: 

                 755     284      59
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0       4      34     124     154     172     202    1098 
   Size:          4      30      90      30      18      30     896

  Fragments arranged by size: 

                 896      90      30      30      30      18       4
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     427    1098 
   Size:        427     671
 
BseYI C'CCAG_C

Cuts at:      0     203    1098 
   Size:        203     895
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     742     775    1098 
   Size:        742      33     323

  Fragments arranged by size: 

                 742     323      33
 
Bsp1286I G_dGCh'C

Cuts at:      0      40    1098 
   Size:         40    1058
 
BspHI T'CATG_A

Cuts at:      0     363    1098 
   Size:        363     735
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     477    1098 
   Size:        477     621
 
BsrDI GCAATG_nn'

Cuts at:      0     257    1098 
   Size:        257     841
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     750    1098 
   Size:        750     348
 
BtsI GCAGTG_nn'

Cuts at:      0     872     924    1098 
   Size:        872      52     174

  Fragments arranged by size: 

                 872     174      52
 
DraI TTT'AAA

Cuts at:      0     374    1098 
   Size:        374     724
 
EarI CTCTTCn'nnn_

Cuts at:      0     850    1098 
   Size:        850     248
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     256    1098 
   Size:        256     842
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     133     391     627     840    1098 
   Size:        133     258     236     213     258

  Fragments arranged by size: 

                 258     258     236     213     133
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     409    1098 
   Size:        409     689
 
EcoRI G'AATT_C

Cuts at:      0    1024    1098 
   Size:       1024      74
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     240     601    1098 
   Size:        240     361     497

  Fragments arranged by size: 

                 497     361     240
 
MslI CAynn'nnrTG

Cuts at:      0     949    1098 
   Size:        949     149
 
MspA1I CmG'CkG

Cuts at:      0     789    1098 
   Size:        789     309
 
NspI r_CATG'y

Cuts at:      0     131     351     695     885    1098 
   Size:        131     220     344     190     213

  Fragments arranged by size: 

                 344     220     213     190     131
 
PfoI T'CCnGG_A

Cuts at:      0     176    1098 
   Size:        176     922
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     827     859    1098 
   Size:        827      32     239

  Fragments arranged by size: 

                 827     239      32
 
PshAI GACnn'nnGTC

Cuts at:      0      68    1098 
   Size:         68    1030
 
PstI C_TGCA'G

Cuts at:      0     700    1098 
   Size:        700     398
 
PvuII CAG'CTG

Cuts at:      0     789    1098 
   Size:        789     309
 
SapI GCTCTTCn'nnn_

Cuts at:      0     850    1098 
   Size:        850     248
 
SfcI C'TryA_G

Cuts at:      0     696    1098 
   Size:        696     402
 
SmlI C'TyrA_G

Cuts at:      0    1010    1098 
   Size:       1010      88
 
SspI AAT'ATT

Cuts at:      0     679    1098 
   Size:        679     419
 
TatI w'GTAC_w

Cuts at:      0     158     776    1098 
   Size:        158     618     322

  Fragments arranged by size: 

                 618     322     158
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      30     494    1098 
   Size:         30     464     604

  Fragments arranged by size: 

                 604     464      30
 
XmnI GAAnn'nnTTC

Cuts at:      0     581    1098 
   Size:        581     517
 
ZraI GAC'GTC

Cuts at:      0    1040    1098 
   Size:       1040      58
 

 Enzymes that do cut:

    AatII     AcuI     AleI     AlfI    AlwNI     ApoI     BanI     BbsI
 Bme1580I    BmgBI     BpmI    BpuEI    BsaHI    BsaXI    BseRI    BseYI
     BsgI Bsp1286I    BspHI    BspMI    BsrDI    BstXI     BtsI     DraI
     EarI     EciI  Eco57MI    EcoNI    EcoRI     FalI     MslI   MspA1I
     NspI     PfoI     PpiI    PshAI     PstI    PvuII     SapI     SfcI
     SmlI     SspI     TatI     XcmI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI     AvaI
    AvrII     BaeI    BamHI    BanII     BbeI    BbvCI     BcgI    BciVI
     BclI    BfrBI     BglI    BglII     BlpI     BmrI     BmtI     BplI
   Bpu10I     BsaI    BsaAI    BsaBI    BsaWI    BsiEI  BsiHKAI    BsiWI
     BsmI    BsmBI    BspEI    BsrBI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI
     ClaI    CspCI   DraIII     DrdI     EaeI     EagI  EcoICRI EcoO109I
    EcoRV     FseI     FspI    FspAI    HaeII    Hin4I   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PasI     PciI    PflMI     PmeI     PmlI    PpuMI     PsiI
   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI
    SanDI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     StuI     StyI     SwaI
    TaqII     TstI  Tth111I     XbaI     XhoI     XmaI