Restriction map for
pFN21AE3672
(Linear) (Six-base) MAPSORT of: KIEE3672.seq Check: 120 from: 1 to: 1647
KIEE3672.orf
With 182 enzymes: SgfI *
December 27, 2011 16:38 ..
AclI AA'CG_TT
Cuts at: 0 689 1647
Size: 689 958
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 159 547 859 1463 1647
Size: 159 388 312 604 184
Fragments arranged by size:
604 388 312 184 159
AflII C'TTAA_G
Cuts at: 0 1282 1647
Size: 1282 365
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 292 324 1647
Size: 292 32 1323
Fragments arranged by size:
1323 292 32
AlwNI CAG_nnn'CTG
Cuts at: 0 34 1647
Size: 34 1613
ApoI r'AATT_y
Cuts at: 0 520 591 625 935 1293 1313 1647
Size: 520 71 34 310 358 20 334
Fragments arranged by size:
520 358 334 310 71 34 20
AseI AT'TA_AT
Cuts at: 0 662 1647
Size: 662 985
BamHI G'GATC_C
Cuts at: 0 490 1483 1647
Size: 490 993 164
Fragments arranged by size:
993 490 164
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1341 1375 1647
Size: 1341 34 272
Fragments arranged by size:
1341 272 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 226 1647
Size: 226 1421
BglI GCCn_nnn'nGGC
Cuts at: 0 1372 1647
Size: 1372 275
BlpI GC'TnA_GC
Cuts at: 0 1001 1647
Size: 1001 646
BmrI ACTGGGnnnn_n'
Cuts at: 0 1019 1647
Size: 1019 628
BmtI G_CTAG'C
Cuts at: 0 163 1647
Size: 163 1484
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 500 1091 1647
Size: 500 591 556
Fragments arranged by size:
591 556 500
BsaI GGTCTCn'nnnn_
Cuts at: 0 970 1647
Size: 970 677
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 363 393 1378 1408 1647
Size: 363 30 985 30 239
Fragments arranged by size:
985 363 239 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 985 988 1647
Size: 985 3 659
Fragments arranged by size:
985 659 3
BseYI C'CCAG_C
Cuts at: 0 222 316 339 1647
Size: 222 94 23 1308
Fragments arranged by size:
1308 222 94 23
BsiHKAI G_wGCw'C
Cuts at: 0 1586 1647
Size: 1586 61
BsmI GAATG_Cn'
Cuts at: 0 388 716 1112 1647
Size: 388 328 396 535
Fragments arranged by size:
535 396 388 328
Bsp1286I G_dGCh'C
Cuts at: 0 1586 1647
Size: 1586 61
BspHI T'CATG_A
Cuts at: 0 1289 1647
Size: 1289 358
BsrGI T'GTAC_A
Cuts at: 0 115 1647
Size: 115 1532
BstAPI GCAn_nnn'nTGC
Cuts at: 0 199 819 1647
Size: 199 620 828
Fragments arranged by size:
828 620 199
BstYI r'GATC_y
Cuts at: 0 490 1483 1647
Size: 490 993 164
Fragments arranged by size:
993 490 164
BtgI C'CryG_G
Cuts at: 0 1040 1647
Size: 1040 607
BtsI GCAGTG_nn'
Cuts at: 0 456 1647
Size: 456 1191
DraI TTT'AAA
Cuts at: 0 596 1647
Size: 596 1051
EarI CTCTTCn'nnn_
Cuts at: 0 747 1647
Size: 747 900
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 159 500 547 859 1091 1463 1647
Size: 159 341 47 312 232 372 184
Fragments arranged by size:
372 341 312 232 184 159 47
EcoRI G'AATT_C
Cuts at: 0 935 1293 1647
Size: 935 358 354
Fragments arranged by size:
935 358 354
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 363 395 1378 1410 1647
Size: 363 32 983 32 237
Fragments arranged by size:
983 363 237 32 32
HindIII A'AGCT_T
Cuts at: 0 900 1260 1319 1647
Size: 900 360 59 328
Fragments arranged by size:
900 360 328 59
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 806 1647
Size: 806 841
MslI CAynn'nnrTG
Cuts at: 0 145 709 1647
Size: 145 564 938
Fragments arranged by size:
938 564 145
MspA1I CmG'CkG
Cuts at: 0 222 1647
Size: 222 1425
NcoI C'CATG_G
Cuts at: 0 1040 1647
Size: 1040 607
NdeI CA'TA_TG
Cuts at: 0 723 1647
Size: 723 924
NheI G'CTAG_C
Cuts at: 0 159 1647
Size: 159 1488
NspI r_CATG'y
Cuts at: 0 822 1647
Size: 822 825
PasI CC'CwG_GG
Cuts at: 0 1479 1647
Size: 1479 168
PfoI T'CCnGG_A
Cuts at: 0 944 1210 1647
Size: 944 266 437
Fragments arranged by size:
944 437 266
PstI C_TGCA'G
Cuts at: 0 451 1647
Size: 451 1196
PvuII CAG'CTG
Cuts at: 0 222 1647
Size: 222 1425
SapI GCTCTTCn'nnn_
Cuts at: 0 747 1647
Size: 747 900
SexAI A'CCwGG_T
Cuts at: 0 1502 1647
Size: 1502 145
SfcI C'TryA_G
Cuts at: 0 447 1647
Size: 447 1200
SmlI C'TyrA_G
Cuts at: 0 1282 1647
Size: 1282 365
StuI AGG'CCT
Cuts at: 0 1602 1647
Size: 1602 45
StyI C'CwwG_G
Cuts at: 0 607 1040 1541 1647
Size: 607 433 501 106
Fragments arranged by size:
607 501 433 106
TatI w'GTAC_w
Cuts at: 0 115 1647
Size: 115 1532
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 224 256 1647
Size: 224 32 1391
Fragments arranged by size:
1391 224 32
Tth111I GACn'n_nGTC
Cuts at: 0 1504 1647
Size: 1504 143
Enzymes that do cut:
AclI AcuI AflII AloI AlwNI ApoI AseI BamHI
BcgI BciVI BglI BlpI BmrI BmtI BpmI BsaI
BsaXI BseRI BseYI BsiHKAI BsmI Bsp1286I BspHI BsrGI
BstAPI BstYI BtgI BtsI DraI EarI Eco57MI EcoRI
Hin4I HindIII MmeI MslI MspA1I NcoI NdeI NheI
NspI PasI PfoI PstI PvuII SapI SexAI SfcI
SmlI StuI StyI TatI TstI Tth111I
Enzymes that do not cut:
AarI AatII AccI Acc65I AfeI AflIII AgeI AhdI
AleI AlfI ApaI ApaLI AscI AsiSI AvaI AvrII
BaeI BanI BanII BbeI BbsI BbvCI BclI BfrBI
BglII Bme1580I BmgBI BplI Bpu10I BpuEI BsaAI BsaBI
BsaHI BsaWI BsgI BsiEI BsiWI BsmBI BspEI BspMI
BsrBI BsrDI BsrFI BssHII BssSI BstBI BstEII BstXI
BstZ17I Bsu36I BtgZI ClaI CspCI DraIII DrdI EaeI
EagI EciI EcoICRI EcoNI EcoO109I EcoRV FalI FseI
FspI FspAI HaeII HincII HpaI KasI KpnI MfeI
MluI MscI NaeI NarI NgoMIV NotI NruI NsiI
PacI PciI PflMI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII
SalI SanDI SbfI ScaI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI SwaI TaqII
XbaI XcmI XhoI XmaI XmnI ZraI