Restriction map for
pFN21AE3671
(Linear) (Six-base) MAPSORT of: KIEE3671.seq Check: 4602 from: 1 to: 2910
KIEE3671.orf
With 182 enzymes: SgfI *
March 22, 2012 16:39 ..
AccI GT'mk_AC
Cuts at: 0 77 393 2910
Size: 77 316 2517
Fragments arranged by size:
2517 316 77
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 755 992 1894 2910
Size: 755 237 902 1016
Fragments arranged by size:
1016 902 755 237
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1472 2540 2910
Size: 1472 1068 370
Fragments arranged by size:
1472 1068 370
AlwNI CAG_nnn'CTG
Cuts at: 0 2602 2862 2910
Size: 2602 260 48
Fragments arranged by size:
2602 260 48
ApaLI G'TGCA_C
Cuts at: 0 557 2910
Size: 557 2353
ApoI r'AATT_y
Cuts at: 0 801 2148 2910
Size: 801 1347 762
Fragments arranged by size:
1347 801 762
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1104 1137 1553 1586 2910
Size: 1104 33 416 33 1324
Fragments arranged by size:
1324 1104 416 33 33
BanI G'GyrC_C
Cuts at: 0 2541 2910
Size: 2541 369
BbsI GAAGACnn'nnnn_
Cuts at: 0 761 845 936 2632 2762 2858 2910
Size: 761 84 91 1696 130 96 52
Fragments arranged by size:
1696 761 130 96 91 84 52
BfrBI ATG'CAT
Cuts at: 0 1882 2135 2517 2910
Size: 1882 253 382 393
Fragments arranged by size:
1882 393 382 253
BglII A'GATC_T
Cuts at: 0 1141 2089 2910
Size: 1141 948 821
Fragments arranged by size:
1141 948 821
Bme1580I G_kGCm'C
Cuts at: 0 561 2910
Size: 561 2349
BmrI ACTGGGnnnn_n'
Cuts at: 0 2739 2910
Size: 2739 171
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1275 2910
Size: 1275 1635
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1151 1871 2910
Size: 1151 720 1039
Fragments arranged by size:
1151 1039 720
BsaI GGTCTCn'nnnn_
Cuts at: 0 615 2910
Size: 615 2295
BsaBI GATnn'nnATC
Cuts at: 0 1346 2094 2910
Size: 1346 748 816
Fragments arranged by size:
1346 816 748
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1250 1864 2302 2444 2910
Size: 1250 614 438 142 466
Fragments arranged by size:
1250 614 466 438 142
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1625 2776 2910
Size: 1625 1151 134
Fragments arranged by size:
1625 1151 134
BsiHKAI G_wGCw'C
Cuts at: 0 561 2388 2910
Size: 561 1827 522
Fragments arranged by size:
1827 561 522
Bsp1286I G_dGCh'C
Cuts at: 0 561 2388 2910
Size: 561 1827 522
Fragments arranged by size:
1827 561 522
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 16 2910
Size: 16 2894
BsrDI GCAATG_nn'
Cuts at: 0 189 2910
Size: 189 2721
BsrGI T'GTAC_A
Cuts at: 0 143 2076 2910
Size: 143 1933 834
Fragments arranged by size:
1933 834 143
BstYI r'GATC_y
Cuts at: 0 1141 1324 1692 2089 2910
Size: 1141 183 368 397 821
Fragments arranged by size:
1141 821 397 368 183
BstZ17I GTA'TAC
Cuts at: 0 394 2910
Size: 394 2516
Bsu36I CC'TnA_GG
Cuts at: 0 2438 2910
Size: 2438 472
BtgI C'CryG_G
Cuts at: 0 1735 2910
Size: 1735 1175
DraI TTT'AAA
Cuts at: 0 36 1299 2255 2910
Size: 36 1263 956 655
Fragments arranged by size:
1263 956 655 36
DrdI GACnn_nn'nnGTC
Cuts at: 0 2241 2910
Size: 2241 669
EarI CTCTTCn'nnn_
Cuts at: 0 1068 1244 2357 2622 2910
Size: 1068 176 1113 265 288
Fragments arranged by size:
1113 1068 288 265 176
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 755 992 1894 2910
Size: 755 237 902 1016
Fragments arranged by size:
1016 902 755 237
EcoO109I rG'GnC_Cy
Cuts at: 0 405 717 2741 2910
Size: 405 312 2024 169
Fragments arranged by size:
2024 405 312 169
EcoRI G'AATT_C
Cuts at: 0 2148 2910
Size: 2148 762
EcoRV GAT'ATC
Cuts at: 0 1916 2910
Size: 1916 994
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 785 1924 2656 2910
Size: 785 1139 732 254
Fragments arranged by size:
1139 785 732 254
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 360 392 568 600 1867 1899 2479 2511
Size: 360 32 176 32 1267 32 580 32
Cuts at: 2511 2910
Size: 399
Fragments arranged by size:
1267 580 399 360 176 32 32 32
32
HincII GTy'rAC
Cuts at: 0 2705 2910
Size: 2705 205
HpaI GTT'AAC
Cuts at: 0 2705 2910
Size: 2705 205
MfeI C'AATT_G
Cuts at: 0 196 845 2910
Size: 196 649 2065
Fragments arranged by size:
2065 649 196
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 21 1692 2557 2910
Size: 21 1671 865 353
Fragments arranged by size:
1671 865 353 21
MslI CAynn'nnrTG
Cuts at: 0 367 386 2140 2221 2315 2910
Size: 367 19 1754 81 94 595
Fragments arranged by size:
1754 595 367 94 81 19
NcoI C'CATG_G
Cuts at: 0 1735 2910
Size: 1735 1175
NsiI A_TGCA'T
Cuts at: 0 1884 2137 2519 2910
Size: 1884 253 382 391
Fragments arranged by size:
1884 391 382 253
NspI r_CATG'y
Cuts at: 0 862 2910
Size: 862 2048
PasI CC'CwG_GG
Cuts at: 0 588 2910
Size: 588 2322
PflMI CCAn_nnn'nTGG
Cuts at: 0 1957 2910
Size: 1957 953
PfoI T'CCnGG_A
Cuts at: 0 1374 2910
Size: 1374 1536
PpuMI rG'GwC_Cy
Cuts at: 0 405 717 2741 2910
Size: 405 312 2024 169
Fragments arranged by size:
2024 405 312 169
PsiI TTA'TAA
Cuts at: 0 227 2910
Size: 227 2683
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 2415 2447 2910
Size: 2415 32 463
Fragments arranged by size:
2415 463 32
SanDI GG'GwC_CC
Cuts at: 0 2741 2910
Size: 2741 169
ScaI AGT'ACT
Cuts at: 0 2427 2910
Size: 2427 483
SexAI A'CCwGG_T
Cuts at: 0 2418 2910
Size: 2418 492
SfcI C'TryA_G
Cuts at: 0 78 2594 2910
Size: 78 2516 316
Fragments arranged by size:
2516 316 78
SmlI C'TyrA_G
Cuts at: 0 1166 1886 2910
Size: 1166 720 1024
Fragments arranged by size:
1166 1024 720
SpeI A'CTAG_T
Cuts at: 0 2428 2707 2910
Size: 2428 279 203
Fragments arranged by size:
2428 279 203
SphI G_CATG'C
Cuts at: 0 862 2910
Size: 862 2048
StuI AGG'CCT
Cuts at: 0 2443 2910
Size: 2443 467
StyI C'CwwG_G
Cuts at: 0 713 1735 2910
Size: 713 1022 1175
Fragments arranged by size:
1175 1022 713
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1278 2910
Size: 1278 1632
TatI w'GTAC_w
Cuts at: 0 143 2076 2425 2910
Size: 143 1933 349 485
Fragments arranged by size:
1933 485 349 143
Tth111I GACn'n_nGTC
Cuts at: 0 643 2582 2910
Size: 643 1939 328
Fragments arranged by size:
1939 643 328
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1824 2042 2910
Size: 1824 218 868
Fragments arranged by size:
1824 868 218
XmnI GAAnn'nnTTC
Cuts at: 0 319 804 1907 2910
Size: 319 485 1103 1003
Fragments arranged by size:
1103 1003 485 319
Enzymes that do cut:
AccI AcuI AlfI AlwNI ApaLI ApoI BaeI BanI
BbsI BfrBI BglII Bme1580I BmrI BplI BpuEI BsaI
BsaBI BseRI BsgI BsiHKAI Bsp1286I BspMI BsrDI BsrGI
BstYI BstZ17I Bsu36I BtgI DraI DrdI EarI Eco57MI
EcoO109I EcoRI EcoRV FalI Hin4I HincII HpaI MfeI
MmeI MslI NcoI NsiI NspI PasI PflMI PfoI
PpuMI PsiI PsrI SanDI ScaI SexAI SfcI SmlI
SpeI SphI StuI StyI TaqII TatI Tth111I XcmI
XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AloI ApaI AscI AseI AsiSI AvaI
AvrII BamHI BanII BbeI BbvCI BcgI BciVI BclI
BglI BlpI BmgBI BmtI BpmI Bpu10I BsaAI BsaHI
BsaWI BsaXI BseYI BsiEI BsiWI BsmI BsmBI BspEI
BspHI BsrBI BsrFI BssHII BssSI BstAPI BstBI BstEII
BstXI BtgZI BtsI ClaI CspCI DraIII EaeI EagI
EciI EcoICRI EcoNI FseI FspI FspAI HaeII HindIII
KasI KpnI MluI MscI MspA1I NaeI NarI NdeI
NgoMIV NheI NotI NruI PacI PciI PmeI PmlI
PpiI PshAI PspOMI PspXI PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI SfiI SfoI SgfI
SgrAI SmaI SnaBI SrfI SspI SwaI TstI XbaI
XhoI XmaI ZraI