Restriction map for
pFN21AE3670
(Linear) (Six-base) MAPSORT of: KIEE3670.seq Check: 4070 from: 1 to: 1479
KIEE3670.orf
With 182 enzymes: SgfI *
December 27, 2011 16:38 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 627 1479
Size: 627 852
AflIII A'CryG_T
Cuts at: 0 384 1479
Size: 384 1095
AleI CACnn'nnGTG
Cuts at: 0 413 1479
Size: 413 1066
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 952 1479
Size: 952 527
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 163 195 1479
Size: 163 32 1284
Fragments arranged by size:
1284 163 32
AlwNI CAG_nnn'CTG
Cuts at: 0 491 1479
Size: 491 988
AvaI C'yCGr_G
Cuts at: 0 166 359 1479
Size: 166 193 1120
Fragments arranged by size:
1120 193 166
BanI G'GyrC_C
Cuts at: 0 211 1479
Size: 211 1268
BanII G_rGCy'C
Cuts at: 0 732 1479
Size: 732 747
BbsI GAAGACnn'nnnn_
Cuts at: 0 92 756 997 1479
Size: 92 664 241 482
Fragments arranged by size:
664 482 241 92
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 725 759 1479
Size: 725 34 720
Fragments arranged by size:
725 720 34
BfrBI ATG'CAT
Cuts at: 0 1291 1428 1479
Size: 1291 137 51
Fragments arranged by size:
1291 137 51
BlpI GC'TnA_GC
Cuts at: 0 237 1343 1479
Size: 237 1106 136
Fragments arranged by size:
1106 237 136
Bme1580I G_kGCm'C
Cuts at: 0 216 685 1479
Size: 216 469 794
Fragments arranged by size:
794 469 216
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 658 1369 1479
Size: 658 711 110
Fragments arranged by size:
711 658 110
Bpu10I CC'TnA_GC
Cuts at: 0 554 1479
Size: 554 925
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1069 1479
Size: 1069 410
BsaAI yAC'GTr
Cuts at: 0 117 1479
Size: 117 1362
BsaWI w'CCGG_w
Cuts at: 0 818 1306 1479
Size: 818 488 173
Fragments arranged by size:
818 488 173
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 163 193 1479
Size: 163 30 1286
Fragments arranged by size:
1286 163 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 184 1461 1479
Size: 184 1277 18
Fragments arranged by size:
1277 184 18
BseYI C'CCAG_C
Cuts at: 0 491 1479
Size: 491 988
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 437 710 860 1038 1409 1479
Size: 437 273 150 178 371 70
Fragments arranged by size:
437 371 273 178 150 70
BsiHKAI G_wGCw'C
Cuts at: 0 227 732 876 1479
Size: 227 505 144 603
Fragments arranged by size:
603 505 227 144
BsmI GAATG_Cn'
Cuts at: 0 1239 1479
Size: 1239 240
Bsp1286I G_dGCh'C
Cuts at: 0 216 227 685 732 876 1479
Size: 216 11 458 47 144 603
Fragments arranged by size:
603 458 216 144 47 11
BspEI T'CCGG_A
Cuts at: 0 818 1306 1479
Size: 818 488 173
Fragments arranged by size:
818 488 173
BspHI T'CATG_A
Cuts at: 0 1190 1479
Size: 1190 289
BsrDI GCAATG_nn'
Cuts at: 0 42 595 946 1479
Size: 42 553 351 533
Fragments arranged by size:
553 533 351 42
BssSI C'ACGA_G
Cuts at: 0 1168 1479
Size: 1168 311
BstAPI GCAn_nnn'nTGC
Cuts at: 0 435 752 905 1479
Size: 435 317 153 574
Fragments arranged by size:
574 435 317 153
BstXI CCAn_nnnn'nTGG
Cuts at: 0 135 1248 1479
Size: 135 1113 231
Fragments arranged by size:
1113 231 135
BtgI C'CryG_G
Cuts at: 0 701 1479
Size: 701 778
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 146 1479
Size: 146 1333
BtsI GCAGTG_nn'
Cuts at: 0 425 501 1008 1479
Size: 425 76 507 471
Fragments arranged by size:
507 471 425 76
DraIII CAC_nnn'GTG
Cuts at: 0 836 1479
Size: 836 643
EaeI y'GGCC_r
Cuts at: 0 1250 1479
Size: 1250 229
EarI CTCTTCn'nnn_
Cuts at: 0 35 62 646 1479
Size: 35 27 584 833
Fragments arranged by size:
833 584 35 27
EcoICRI GAG'CTC
Cuts at: 0 730 1479
Size: 730 749
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 627 658 1369 1479
Size: 627 31 711 110
Fragments arranged by size:
711 627 110 31
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1110 1479
Size: 1110 369
HaeII r_GCGC'y
Cuts at: 0 636 1479
Size: 636 843
HindIII A'AGCT_T
Cuts at: 0 1131 1479
Size: 1131 348
MscI TGG'CCA
Cuts at: 0 1252 1479
Size: 1252 227
MslI CAynn'nnrTG
Cuts at: 0 413 1001 1479
Size: 413 588 478
Fragments arranged by size:
588 478 413
MspA1I CmG'CkG
Cuts at: 0 488 678 1161 1347 1443 1479
Size: 488 190 483 186 96 36
Fragments arranged by size:
488 483 190 186 96 36
NcoI C'CATG_G
Cuts at: 0 701 1479
Size: 701 778
NdeI CA'TA_TG
Cuts at: 0 1287 1479
Size: 1287 192
NsiI A_TGCA'T
Cuts at: 0 1293 1430 1479
Size: 1293 137 49
Fragments arranged by size:
1293 137 49
NspI r_CATG'y
Cuts at: 0 388 939 1479
Size: 388 551 540
Fragments arranged by size:
551 540 388
PasI CC'CwG_GG
Cuts at: 0 94 1479
Size: 94 1385
PciI A'CATG_T
Cuts at: 0 384 1479
Size: 384 1095
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 163 195 1479
Size: 163 32 1284
Fragments arranged by size:
1284 163 32
PspXI vC'TCGA_Gb
Cuts at: 0 359 1479
Size: 359 1120
PvuII CAG'CTG
Cuts at: 0 488 678 1347 1479
Size: 488 190 669 132
Fragments arranged by size:
669 488 190 132
SacI G_AGCT'C
Cuts at: 0 732 1479
Size: 732 747
ScaI AGT'ACT
Cuts at: 0 1093 1479
Size: 1093 386
SfcI C'TryA_G
Cuts at: 0 1228 1479
Size: 1228 251
SmlI C'TyrA_G
Cuts at: 0 359 1084 1479
Size: 359 725 395
Fragments arranged by size:
725 395 359
StyI C'CwwG_G
Cuts at: 0 701 1479
Size: 701 778
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 813 1479
Size: 813 666
TatI w'GTAC_w
Cuts at: 0 738 1091 1479
Size: 738 353 388
Fragments arranged by size:
738 388 353
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 384 416 1479
Size: 384 32 1063
Fragments arranged by size:
1063 384 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 682 1479
Size: 682 797
XhoI C'TCGA_G
Cuts at: 0 359 1479
Size: 359 1120
XmnI GAAnn'nnTTC
Cuts at: 0 1465 1479
Size: 1465 14
Enzymes that do cut:
AcuI AflIII AleI AlfI AloI AlwNI AvaI BanI
BanII BbsI BcgI BfrBI BlpI Bme1580I BpmI Bpu10I
BpuEI BsaAI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI
BsmI Bsp1286I BspEI BspHI BsrDI BssSI BstAPI BstXI
BtgI BtgZI BtsI DraIII EaeI EarI EcoICRI Eco57MI
FalI HaeII HindIII MscI MslI MspA1I NcoI NdeI
NsiI NspI PasI PciI PpiI PspXI PvuII SacI
ScaI SfcI SmlI StyI TaqII TatI TstI XcmI
XhoI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI ApaI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BbvCI BciVI BclI BglI BglII
BmgBI BmrI BmtI BplI BsaI BsaBI BsaHI BsiEI
BsiWI BsmBI BspMI BsrBI BsrFI BsrGI BssHII BstBI
BstEII BstYI BstZ17I Bsu36I ClaI CspCI DraI DrdI
EagI EciI EcoNI EcoO109I EcoRI EcoRV FseI FspI
FspAI Hin4I HincII HpaI KasI KpnI MfeI MluI
MmeI NaeI NarI NgoMIV NheI NotI NruI PacI
PflMI PfoI PmeI PmlI PpuMI PshAI PsiI PspOMI
PsrI PstI PvuI RsrII SacII SalI SanDI SapI
SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI StuI SwaI Tth111I XbaI
XmaI ZraI