Restriction map for
pFN21AE3664
(Linear) (Six-base) MAPSORT of: KIEE3664.seq Check: 1857 from: 1 to: 1962
KIEE3664.orf
With 182 enzymes: SgfI *
March 22, 2012 16:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1543 1562 1863 1962
Size: 1543 19 301 99
Fragments arranged by size:
1543 301 99 19
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 269 647 1032 1761 1962
Size: 269 378 385 729 201
Fragments arranged by size:
729 385 378 269 201
AfeI AGC'GCT
Cuts at: 0 454 1045 1962
Size: 454 591 917
Fragments arranged by size:
917 591 454
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1594 1962
Size: 1594 368
AlwNI CAG_nnn'CTG
Cuts at: 0 54 219 234 696 1962
Size: 54 165 15 462 1266
Fragments arranged by size:
1266 462 165 54 15
ApaI G_GGCC'C
Cuts at: 0 381 1351 1470 1962
Size: 381 970 119 492
Fragments arranged by size:
970 492 381 119
ApaLI G'TGCA_C
Cuts at: 0 1696 1962
Size: 1696 266
AvaI C'yCGr_G
Cuts at: 0 179 260 362 499 677 732 831 972
Size: 179 81 102 137 178 55 99 141
Cuts at: 972 1517 1947 1962
Size: 545 430 15
Fragments arranged by size:
545 430 179 178 141 137 102 99
81 55 15
AvrII C'CTAG_G
Cuts at: 0 79 1644 1962
Size: 79 1565 318
Fragments arranged by size:
1565 318 79
BanI G'GyrC_C
Cuts at: 0 1570 1962
Size: 1570 392
BanII G_rGCy'C
Cuts at: 0 381 770 979 1351 1470 1496 1961 1962
Size: 381 389 209 372 119 26 465 1
Fragments arranged by size:
465 389 381 372 209 119 26 1
BbsI GAAGACnn'nnnn_
Cuts at: 0 1406 1962
Size: 1406 556
BglI GCCn_nnn'nGGC
Cuts at: 0 1646 1962
Size: 1646 316
BlpI GC'TnA_GC
Cuts at: 0 697 1962
Size: 697 1265
Bme1580I G_kGCm'C
Cuts at: 0 381 1068 1084 1351 1470 1573 1700 1962
Size: 381 687 16 267 119 103 127 262
Fragments arranged by size:
687 381 267 262 127 119 103 16
BmrI ACTGGGnnnn_n'
Cuts at: 0 795 1087 1377 1599 1940 1962
Size: 795 292 290 222 341 22
Fragments arranged by size:
795 341 292 290 222 22
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 183 1962
Size: 183 1779
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1621 1962
Size: 1621 341
Bpu10I CC'TnA_GC
Cuts at: 0 101 1962
Size: 101 1861
BsaI GGTCTCn'nnnn_
Cuts at: 0 294 735 1962
Size: 294 441 1227
Fragments arranged by size:
1227 441 294
BsaAI yAC'GTr
Cuts at: 0 609 1962
Size: 609 1353
BsaHI Gr'CG_yC
Cuts at: 0 1122 1962
Size: 1122 840
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 940 970 1920 1950 1962
Size: 940 30 950 30 12
Fragments arranged by size:
950 940 30 30 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 37 139 1962
Size: 37 102 1823
Fragments arranged by size:
1823 102 37
BseYI C'CCAG_C
Cuts at: 0 65 174 449 455 631 1089 1218 1283
Size: 65 109 275 6 176 458 129 65
Cuts at: 1283 1360 1378 1566 1636 1706 1797 1962
Size: 77 18 188 70 70 91 165
Fragments arranged by size:
458 275 188 176 165 129 109 91
77 70 70 65 65 18 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 205 285 1090 1962
Size: 205 80 805 872
Fragments arranged by size:
872 805 205 80
BsiEI CG_ry'CG
Cuts at: 0 538 1962
Size: 538 1424
BsiHKAI G_wGCw'C
Cuts at: 0 88 1619 1700 1856 1962
Size: 88 1531 81 156 106
Fragments arranged by size:
1531 156 106 88 81
BsmBI CGTCTCn'nnnn_
Cuts at: 0 731 1461 1962
Size: 731 730 501
Fragments arranged by size:
731 730 501
Bsp1286I G_dGCh'C
Cuts at: 0 88 381 770 979 1068 1084 1351 1470
Size: 88 293 389 209 89 16 267 119
Cuts at: 1470 1496 1573 1619 1700 1856 1961 1962
Size: 26 77 46 81 156 105 1
Fragments arranged by size:
389 293 267 209 156 119 105 89
88 81 77 46 26 16 1
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 533 959 1161 1274 1543 1562 1863 1962
Size: 533 426 202 113 269 19 301 99
Fragments arranged by size:
533 426 301 269 202 113 99 19
BsrBI CCG'CTC
Cuts at: 0 147 1836 1962
Size: 147 1689 126
Fragments arranged by size:
1689 147 126
BsrFI r'CCGG_y
Cuts at: 0 368 1962
Size: 368 1594
BssHII G'CGCG_C
Cuts at: 0 1675 1962
Size: 1675 287
BssSI C'ACGA_G
Cuts at: 0 1184 1962
Size: 1184 778
BstAPI GCAn_nnn'nTGC
Cuts at: 0 219 1962
Size: 219 1743
BtgI C'CryG_G
Cuts at: 0 72 107 153 1116 1229 1757 1808 1962
Size: 72 35 46 963 113 528 51 154
Fragments arranged by size:
963 528 154 113 72 51 46 35
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 194 1962
Size: 194 1768
BtsI GCAGTG_nn'
Cuts at: 0 461 493 598 860 1068 1102 1405 1921
Size: 461 32 105 262 208 34 303 516
Cuts at: 1921 1962
Size: 41
Fragments arranged by size:
516 461 303 262 208 105 41 34
32
EaeI y'GGCC_r
Cuts at: 0 281 535 845 936 1625 1754 1962
Size: 281 254 310 91 689 129 208
Fragments arranged by size:
689 310 281 254 208 129 91
EagI C'GGCC_G
Cuts at: 0 535 1962
Size: 535 1427
EarI CTCTTCn'nnn_
Cuts at: 0 425 1277 1962
Size: 425 852 685
Fragments arranged by size:
852 685 425
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1813 1962
Size: 1813 149
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 269 647 1032 1621 1761 1962
Size: 269 378 385 589 140 201
Fragments arranged by size:
589 385 378 269 201 140
EcoO109I rG'GnC_Cy
Cuts at: 0 216 257 377 579 1052 1347 1466 1467
Size: 216 41 120 202 473 295 119 1
Cuts at: 1467 1526 1962
Size: 59 436
Fragments arranged by size:
473 436 295 216 202 120 119 59
41 1
EcoRV GAT'ATC
Cuts at: 0 1032 1962
Size: 1032 930
HaeII r_GCGC'y
Cuts at: 0 456 1047 1652 1962
Size: 456 591 605 310
Fragments arranged by size:
605 591 456 310
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 940 972 1962
Size: 940 32 990
Fragments arranged by size:
990 940 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1175 1962
Size: 1175 787
MscI TGG'CCA
Cuts at: 0 938 1627 1756 1962
Size: 938 689 129 206
Fragments arranged by size:
938 689 206 129
MspA1I CmG'CkG
Cuts at: 0 231 334 983 1118 1392 1566 1578 1797
Size: 231 103 649 135 274 174 12 219
Cuts at: 1797 1810 1962
Size: 13 152
Fragments arranged by size:
649 274 231 219 174 152 135 103
13 12
NaeI GCC'GGC
Cuts at: 0 370 1962
Size: 370 1592
NcoI C'CATG_G
Cuts at: 0 107 153 1229 1757 1962
Size: 107 46 1076 528 205
Fragments arranged by size:
1076 528 205 107 46
NgoMIV G'CCGG_C
Cuts at: 0 368 1962
Size: 368 1594
NspI r_CATG'y
Cuts at: 0 1675 1962
Size: 1675 287
PasI CC'CwG_GG
Cuts at: 0 885 1962
Size: 885 1077
PflMI CCAn_nnn'nTGG
Cuts at: 0 945 1489 1962
Size: 945 544 473
Fragments arranged by size:
945 544 473
PmlI CAC'GTG
Cuts at: 0 609 1962
Size: 609 1353
PspOMI G'GGCC_C
Cuts at: 0 377 1347 1466 1962
Size: 377 970 119 496
Fragments arranged by size:
970 496 377 119
PvuII CAG'CTG
Cuts at: 0 1797 1962
Size: 1797 165
SacII CC_GC'GG
Cuts at: 0 1119 1811 1962
Size: 1119 692 151
Fragments arranged by size:
1119 692 151
SexAI A'CCwGG_T
Cuts at: 0 1655 1962
Size: 1655 307
SfcI C'TryA_G
Cuts at: 0 1891 1962
Size: 1891 71
SmaI CCC'GGG
Cuts at: 0 262 501 679 1519 1949 1962
Size: 262 239 178 840 430 13
Fragments arranged by size:
840 430 262 239 178 13
SphI G_CATG'C
Cuts at: 0 1675 1962
Size: 1675 287
StyI C'CwwG_G
Cuts at: 0 79 107 153 1055 1229 1433 1644 1757
Size: 79 28 46 902 174 204 211 113
Cuts at: 1757 1962
Size: 205
Fragments arranged by size:
902 211 205 204 174 113 79 46
28
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 956 1581 1698 1962
Size: 956 625 117 264
Fragments arranged by size:
956 625 264 117
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1920 1952 1962
Size: 1920 32 10
Fragments arranged by size:
1920 32 10
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 160 1962
Size: 160 1802
XmaI C'CCGG_G
Cuts at: 0 260 499 677 1517 1947 1962
Size: 260 239 178 840 430 15
Fragments arranged by size:
840 430 260 239 178 15
Enzymes that do cut:
AarI AcuI AfeI AlfI AlwNI ApaI ApaLI AvaI
AvrII BanI BanII BbsI BglI BlpI Bme1580I BmrI
BplI BpmI Bpu10I BsaI BsaAI BsaHI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI
BsrFI BssHII BssSI BstAPI BtgI BtgZI BtsI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRV HaeII Hin4I
MmeI MscI MspA1I NaeI NcoI NgoMIV NspI PasI
PflMI PmlI PspOMI PvuII SacII SexAI SfcI SmaI
SphI StyI TaqII TstI XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AflIII AgeI AhdI
AleI AloI ApoI AscI AseI AsiSI BaeI BamHI
BbeI BbvCI BcgI BciVI BclI BfrBI BglII BmgBI
BmtI BpuEI BsaBI BsaWI BsiWI BsmI BspEI BspHI
BsrDI BsrGI BstBI BstEII BstXI BstYI BstZ17I Bsu36I
ClaI CspCI DraI DraIII DrdI EcoICRI EcoNI EcoRI
FalI FseI FspI FspAI HincII HindIII HpaI KasI
KpnI MfeI MluI MslI NarI NdeI NheI NotI
NruI NsiI PacI PciI PfoI PmeI PpiI PpuMI
PshAI PsiI PspXI PsrI PstI PvuI RsrII SacI
SalI SanDI SapI SbfI ScaI SfiI SfoI SgfI
SgrAI SmlI SnaBI SpeI SrfI SspI StuI SwaI
TatI Tth111I XbaI XhoI XmnI ZraI