(Linear) (Six-base) MAPSORT of: KIEE3663.seq  Check: 3568  from: 1  to: 588



KIEE3663.orf

 With 182 enzymes: SgfI * 

                        December 27, 2011 16:37  ..

ApaI G_GGCC'C

Cuts at:      0     217     588 
   Size:        217     371
 
ApaLI G'TGCA_C

Cuts at:      0     304     588 
   Size:        304     284
 
AvaI C'yCGr_G

Cuts at:      0     209     588 
   Size:        209     379
 
BamHI G'GATC_C

Cuts at:      0     447     588 
   Size:        447     141
 
BanI G'GyrC_C

Cuts at:      0     184     204     240     588 
   Size:        184      20      36     348

  Fragments arranged by size: 

                 348     184      36      20
 
BanII G_rGCy'C

Cuts at:      0     156     217     284     588 
   Size:        156      61      67     304

  Fragments arranged by size: 

                 304     156      67      61
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     343     588 
   Size:        343     245
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     343     588 
   Size:        343     245
 
BclI T'GATC_A

Cuts at:      0     491     588 
   Size:        491      97
 
Bme1580I G_kGCm'C

Cuts at:      0     209     217     308     588 
   Size:        209       8      91     280

  Fragments arranged by size: 

                 280     209      91       8
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     159     588 
   Size:        159     429
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      47     588 
   Size:         47     541
 
BsaAI yAC'GTr

Cuts at:      0     354     588 
   Size:        354     234
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      69     159     337     588 
   Size:         69      90     178     251

  Fragments arranged by size: 

                 251     178      90      69
 
BsiHKAI G_wGCw'C

Cuts at:      0     308     588 
   Size:        308     280
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     169     588 
   Size:        169     419
 
Bsp1286I G_dGCh'C

Cuts at:      0     156     209     217     284     308     588 
   Size:        156      53       8      67      24     280

  Fragments arranged by size: 

                 280     156      67      53      24       8
 
BstYI r'GATC_y

Cuts at:      0     447     588 
   Size:        447     141
 
Bsu36I CC'TnA_GG

Cuts at:      0     190     588 
   Size:        190     398
 
BtsI GCAGTG_nn'

Cuts at:      0     218     588 
   Size:        218     370
 
EaeI y'GGCC_r

Cuts at:      0     178     588 
   Size:        178     410
 
EarI CTCTTCn'nnn_

Cuts at:      0     371     588 
   Size:        371     217
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     162     588 
   Size:        162     426
 
EcoO109I rG'GnC_Cy

Cuts at:      0     213     214     257     510     588 
   Size:        213       1      43     253      78

  Fragments arranged by size: 

                 253     213      78      43       1
 
MscI TGG'CCA

Cuts at:      0     180     588 
   Size:        180     408
 
PasI CC'CwG_GG

Cuts at:      0     274     588 
   Size:        274     314
 
PfoI T'CCnGG_A

Cuts at:      0      62     588 
   Size:         62     526
 
PpuMI rG'GwC_Cy

Cuts at:      0     257     510     588 
   Size:        257     253      78

  Fragments arranged by size: 

                 257     253      78
 
PspOMI G'GGCC_C

Cuts at:      0     213     588 
   Size:        213     375
 
PstI C_TGCA'G

Cuts at:      0     441     588 
   Size:        441     147
 
SfcI C'TryA_G

Cuts at:      0     437     588 
   Size:        437     151
 
SmaI CCC'GGG

Cuts at:      0     211     588 
   Size:        211     377
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     279     311     588 
   Size:        279      32     277

  Fragments arranged by size: 

                 279     277      32
 
XmaI C'CCGG_G

Cuts at:      0     209     588 
   Size:        209     379
 

 Enzymes that do cut:

     ApaI    ApaLI     AvaI    BamHI     BanI    BanII     BbsI    BciVI
     BclI Bme1580I     BmrI     BsaI    BsaAI    BseRI  BsiHKAI    BsmBI
 Bsp1286I    BstYI   Bsu36I     BtsI     EaeI     EarI     EciI EcoO109I
     MscI     PasI     PfoI    PpuMI   PspOMI     PstI     SfcI     SmaI
     TstI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApoI
     AscI     AseI    AsiSI    AvrII     BaeI     BbeI    BbvCI     BcgI
    BfrBI     BglI    BglII     BlpI    BmgBI     BmtI     BplI     BpmI
   Bpu10I    BpuEI    BsaBI    BsaHI    BsaWI    BsaXI    BseYI     BsgI
    BsiEI    BsiWI     BsmI    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
  BstZ17I     BtgI    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI
     EagI  EcoICRI  Eco57MI    EcoNI    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     MslI   MspA1I     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PciI    PflMI     PmeI     PmlI     PpiI    PshAI     PsiI
    PspXI     PsrI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     StyI     SwaI    TaqII     TatI  Tth111I     XbaI     XcmI     XhoI
     XmnI     ZraI