Restriction map for
pFN21AE3660
(Linear) (Six-base) MAPSORT of: KIEE3660.seq Check: 8696 from: 1 to: 1335
KIEE3660.orf
With 182 enzymes: SgfI *
December 27, 2011 16:37 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 491 638 1072 1162 1335
Size: 491 147 434 90 173
Fragments arranged by size:
491 434 173 147 90
AlwNI CAG_nnn'CTG
Cuts at: 0 597 1085 1335
Size: 597 488 250
Fragments arranged by size:
597 488 250
AseI AT'TA_AT
Cuts at: 0 656 1335
Size: 656 679
AvaI C'yCGr_G
Cuts at: 0 1112 1335
Size: 1112 223
BanII G_rGCy'C
Cuts at: 0 428 443 791 987 1036 1335
Size: 428 15 348 196 49 299
Fragments arranged by size:
428 348 299 196 49 15
BbsI GAAGACnn'nnnn_
Cuts at: 0 1306 1335
Size: 1306 29
BbvCI CC'TCA_GC
Cuts at: 0 1096 1335
Size: 1096 239
BclI T'GATC_A
Cuts at: 0 764 1335
Size: 764 571
BglI GCCn_nnn'nGGC
Cuts at: 0 313 367 982 1335
Size: 313 54 615 353
Fragments arranged by size:
615 353 313 54
Bme1580I G_kGCm'C
Cuts at: 0 363 1335
Size: 363 972
BmgBI CAC'GTC
Cuts at: 0 1290 1335
Size: 1290 45
BmrI ACTGGGnnnn_n'
Cuts at: 0 109 687 1335
Size: 109 578 648
Fragments arranged by size:
648 578 109
BmtI G_CTAG'C
Cuts at: 0 208 1335
Size: 208 1127
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 462 1335
Size: 462 873
Bpu10I CC'TnA_GC
Cuts at: 0 1096 1335
Size: 1096 239
BsaWI w'CCGG_w
Cuts at: 0 179 1335
Size: 179 1156
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 169 199 1335
Size: 169 30 1136
Fragments arranged by size:
1136 169 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 435 436 1189 1335
Size: 435 1 753 146
Fragments arranged by size:
753 435 146 1
BseYI C'CCAG_C
Cuts at: 0 188 1238 1335
Size: 188 1050 97
Fragments arranged by size:
1050 188 97
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 111 700 1335
Size: 111 589 635
Fragments arranged by size:
635 589 111
BsiHKAI G_wGCw'C
Cuts at: 0 428 443 752 1335
Size: 428 15 309 583
Fragments arranged by size:
583 428 309 15
BsmI GAATG_Cn'
Cuts at: 0 567 817 1335
Size: 567 250 518
Fragments arranged by size:
567 518 250
BsmBI CGTCTCn'nnnn_
Cuts at: 0 802 1335
Size: 802 533
Bsp1286I G_dGCh'C
Cuts at: 0 363 428 443 752 791 987 1036 1335
Size: 363 65 15 309 39 196 49 299
Fragments arranged by size:
363 309 299 196 65 49 39 15
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1253 1335
Size: 1253 82
BsrDI GCAATG_nn'
Cuts at: 0 548 1335
Size: 548 787
BsrGI T'GTAC_A
Cuts at: 0 989 1335
Size: 989 346
BstAPI GCAn_nnn'nTGC
Cuts at: 0 573 597 1076 1085 1166 1335
Size: 573 24 479 9 81 169
Fragments arranged by size:
573 479 169 81 24 9
BstEII G'GTnAC_C
Cuts at: 0 720 1335
Size: 720 615
BstYI r'GATC_y
Cuts at: 0 182 1335
Size: 182 1153
BtsI GCAGTG_nn'
Cuts at: 0 353 401 1335
Size: 353 48 934
Fragments arranged by size:
934 353 48
DraI TTT'AAA
Cuts at: 0 759 1335
Size: 759 576
DraIII CAC_nnn'GTG
Cuts at: 0 1215 1217 1335
Size: 1215 2 118
Fragments arranged by size:
1215 118 2
EaeI y'GGCC_r
Cuts at: 0 686 779 1206 1335
Size: 686 93 427 129
Fragments arranged by size:
686 427 129 93
EcoICRI GAG'CTC
Cuts at: 0 426 441 1335
Size: 426 15 894
Fragments arranged by size:
894 426 15
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 491 638 1072 1162 1335
Size: 491 147 434 90 173
Fragments arranged by size:
491 434 173 147 90
EcoNI CCTnn'n_nnAGG
Cuts at: 0 82 1335
Size: 82 1253
EcoO109I rG'GnC_Cy
Cuts at: 0 306 1092 1335
Size: 306 786 243
Fragments arranged by size:
786 306 243
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 900 1335
Size: 900 435
HaeII r_GCGC'y
Cuts at: 0 848 1335
Size: 848 487
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 89 121 654 686 1335
Size: 89 32 533 32 649
Fragments arranged by size:
649 533 89 32 32
HincII GTy'rAC
Cuts at: 0 117 1335
Size: 117 1218
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 467 591 1335
Size: 467 124 744
Fragments arranged by size:
744 467 124
MscI TGG'CCA
Cuts at: 0 688 1208 1335
Size: 688 520 127
Fragments arranged by size:
688 520 127
MspA1I CmG'CkG
Cuts at: 0 511 785 1085 1100 1335
Size: 511 274 300 15 235
Fragments arranged by size:
511 300 274 235 15
NheI G'CTAG_C
Cuts at: 0 204 1335
Size: 204 1131
NspI r_CATG'y
Cuts at: 0 1282 1335
Size: 1282 53
PflMI CCAn_nnn'nTGG
Cuts at: 0 195 1335
Size: 195 1140
PstI C_TGCA'G
Cuts at: 0 134 1081 1124 1264 1335
Size: 134 947 43 140 71
Fragments arranged by size:
947 140 134 71 43
PvuII CAG'CTG
Cuts at: 0 511 1085 1100 1335
Size: 511 574 15 235
Fragments arranged by size:
574 511 235 15
SacI G_AGCT'C
Cuts at: 0 428 443 1335
Size: 428 15 892
Fragments arranged by size:
892 428 15
SexAI A'CCwGG_T
Cuts at: 0 83 1335
Size: 83 1252
SfcI C'TryA_G
Cuts at: 0 130 822 1077 1120 1243 1260 1335
Size: 130 692 255 43 123 17 75
Fragments arranged by size:
692 255 130 123 75 43 17
SmaI CCC'GGG
Cuts at: 0 1114 1335
Size: 1114 221
StyI C'CwwG_G
Cuts at: 0 434 725 918 1335
Size: 434 291 193 417
Fragments arranged by size:
434 417 291 193
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 706 1335
Size: 706 629
TatI w'GTAC_w
Cuts at: 0 989 1335
Size: 989 346
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1182 1214 1335
Size: 1182 32 121
Fragments arranged by size:
1182 121 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 196 379 1158 1335
Size: 196 183 779 177
Fragments arranged by size:
779 196 183 177
XmaI C'CCGG_G
Cuts at: 0 1112 1335
Size: 1112 223
Enzymes that do cut:
AcuI AlwNI AseI AvaI BanII BbsI BbvCI BclI
BglI Bme1580I BmgBI BmrI BmtI BplI Bpu10I BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I
BspMI BsrDI BsrGI BstAPI BstEII BstYI BtsI DraI
DraIII EaeI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII
Hin4I HincII MmeI MscI MspA1I NheI NspI PflMI
PstI PvuII SacI SexAI SfcI SmaI StyI TaqII
TatI TstI XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaI ApaLI ApoI
AscI AsiSI AvrII BaeI BamHI BanI BbeI BcgI
BciVI BfrBI BglII BlpI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsiEI BsiWI BspEI BspHI BsrBI BsrFI
BssHII BssSI BstBI BstXI BstZ17I Bsu36I BtgI BtgZI
ClaI CspCI DrdI EagI EarI EciI EcoRI EcoRV
FseI FspI FspAI HindIII HpaI KasI KpnI MfeI
MluI MslI NaeI NarI NcoI NdeI NgoMIV NotI
NruI NsiI PacI PasI PciI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI
RsrII SacII SalI SanDI SapI SbfI ScaI SfiI
SfoI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI
SspI StuI SwaI Tth111I XbaI XhoI XmnI ZraI