(Linear) (Six-base) MAPSORT of: KIEE3659.seq  Check: 5655  from: 1  to: 525



KIEE3659.orf

 With 182 enzymes: SgfI * 

                        December 27, 2011 16:37  ..

AfeI AGC'GCT

Cuts at:      0      71     525 
   Size:         71     454
 
AvaI C'yCGr_G

Cuts at:      0      50     525 
   Size:         50     475
 
AvrII C'CTAG_G

Cuts at:      0      43     525 
   Size:         43     482
 
BamHI G'GATC_C

Cuts at:      0     228     525 
   Size:        228     297
 
BclI T'GATC_A

Cuts at:      0     446     525 
   Size:        446      79
 
BlpI GC'TnA_GC

Cuts at:      0     125     525 
   Size:        125     400
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     264     525 
   Size:        264     261
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     319     525 
   Size:        319     206
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      70      73     513     525 
   Size:         70       3     440      12

  Fragments arranged by size: 

                 440      70      12       3
 
BseYI C'CCAG_C

Cuts at:      0      90     525 
   Size:         90     435
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     421     525 
   Size:        421     104
 
BsiEI CG_ry'CG

Cuts at:      0      64     525 
   Size:         64     461
 
BsiHKAI G_wGCw'C

Cuts at:      0     305     525 
   Size:        305     220
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      43     525 
   Size:         43     482
 
Bsp1286I G_dGCh'C

Cuts at:      0     305     525 
   Size:        305     220
 
BsrFI r'CCGG_y

Cuts at:      0      25      63     525 
   Size:         25      38     462

  Fragments arranged by size: 

                 462      38      25
 
BssHII G'CGCG_C

Cuts at:      0      21     525 
   Size:         21     504
 
BssSI C'ACGA_G

Cuts at:      0     112     525 
   Size:        112     413
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     342     525 
   Size:        342     183
 
BstYI r'GATC_y

Cuts at:      0     228     374     525 
   Size:        228     146     151

  Fragments arranged by size: 

                 228     151     146
 
EaeI y'GGCC_r

Cuts at:      0      61     440     525 
   Size:         61     379      85

  Fragments arranged by size: 

                 379      85      61
 
EagI C'GGCC_G

Cuts at:      0      61     525 
   Size:         61     464
 
EarI CTCTTCn'nnn_

Cuts at:      0     137     525 
   Size:        137     388
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      64     525 
   Size:         64     461
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     319     525 
   Size:        319     206
 
EcoO109I rG'GnC_Cy

Cuts at:      0      47     117     525 
   Size:         47      70     408

  Fragments arranged by size: 

                 408      70      47
 
HaeII r_GCGC'y

Cuts at:      0      73      98     525 
   Size:         73      25     427

  Fragments arranged by size: 

                 427      73      25
 
MscI TGG'CCA

Cuts at:      0     442     525 
   Size:        442      83
 
MspA1I CmG'CkG

Cuts at:      0     190     525 
   Size:        190     335
 
NaeI GCC'GGC

Cuts at:      0      27      65     525 
   Size:         27      38     460

  Fragments arranged by size: 

                 460      38      27
 
NgoMIV G'CCGG_C

Cuts at:      0      25      63     525 
   Size:         25      38     462

  Fragments arranged by size: 

                 462      38      25
 
PpuMI rG'GwC_Cy

Cuts at:      0      47     525 
   Size:         47     478
 
PshAI GACnn'nnGTC

Cuts at:      0      52     190     525 
   Size:         52     138     335

  Fragments arranged by size: 

                 335     138      52
 
PsiI TTA'TAA

Cuts at:      0     215     525 
   Size:        215     310
 
PvuII CAG'CTG

Cuts at:      0     190     525 
   Size:        190     335
 
SgrAI Cr'CCGG_yG

Cuts at:      0      25     525 
   Size:         25     500
 
StuI AGG'CCT

Cuts at:      0     273     525 
   Size:        273     252
 
StyI C'CwwG_G

Cuts at:      0      43     341     525 
   Size:         43     298     184

  Fragments arranged by size: 

                 298     184      43
 
TatI w'GTAC_w

Cuts at:      0     432     525 
   Size:        432      93
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     393     525 
   Size:        393     132
 

 Enzymes that do cut:

     AfeI     AvaI    AvrII    BamHI     BclI     BlpI     BmrI     BpmI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsmBI Bsp1286I    BsrFI
   BssHII    BssSI    BstXI    BstYI     EaeI     EagI     EarI     EciI
  Eco57MI EcoO109I    HaeII     MscI   MspA1I     NaeI   NgoMIV    PpuMI
    PshAI     PsiI    PvuII    SgrAI     StuI     StyI     TatI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     BaeI     BanI    BanII     BbeI
     BbsI    BbvCI     BcgI    BciVI    BfrBI     BglI    BglII Bme1580I
    BmgBI     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BsiWI     BsmI    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrGI   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I
     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MslI     NarI     NcoI     NdeI     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfcI     SfiI     SfoI     SgfI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     SwaI    TaqII     TstI  Tth111I     XbaI
     XhoI     XmaI     XmnI     ZraI