Restriction map for
pFN21AE3657
(Linear) (Six-base) MAPSORT of: KIEE3657.seq Check: 1927 from: 1 to: 1242
KIEE3657.orf
With 182 enzymes: SgfI *
December 27, 2011 16:37 ..
AatII G_ACGT'C
Cuts at: 0 164 188 1242
Size: 164 24 1054
Fragments arranged by size:
1054 164 24
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 34 1242
Size: 34 1208
AflIII A'CryG_T
Cuts at: 0 701 1242
Size: 701 541
AhdI GACnn_n'nnGTC
Cuts at: 0 675 1242
Size: 675 567
AlwNI CAG_nnn'CTG
Cuts at: 0 222 374 816 1242
Size: 222 152 442 426
Fragments arranged by size:
442 426 222 152
ApaI G_GGCC'C
Cuts at: 0 154 804 1242
Size: 154 650 438
Fragments arranged by size:
650 438 154
ApaLI G'TGCA_C
Cuts at: 0 232 1242
Size: 232 1010
ApoI r'AATT_y
Cuts at: 0 66 564 1242
Size: 66 498 678
Fragments arranged by size:
678 498 66
AvaI C'yCGr_G
Cuts at: 0 797 1242
Size: 797 445
BanI G'GyrC_C
Cuts at: 0 487 1138 1242
Size: 487 651 104
Fragments arranged by size:
651 487 104
BanII G_rGCy'C
Cuts at: 0 154 335 398 794 804 1242
Size: 154 181 63 396 10 438
Fragments arranged by size:
438 396 181 154 63 10
BbsI GAAGACnn'nnnn_
Cuts at: 0 368 1242
Size: 368 874
BbvCI CC'TCA_GC
Cuts at: 0 1006 1121 1242
Size: 1006 115 121
Fragments arranged by size:
1006 121 115
BglI GCCn_nnn'nGGC
Cuts at: 0 1033 1049 1219 1228 1242
Size: 1033 16 170 9 14
Fragments arranged by size:
1033 170 16 14 9
BlpI GC'TnA_GC
Cuts at: 0 40 1242
Size: 40 1202
Bme1580I G_kGCm'C
Cuts at: 0 154 236 804 1242
Size: 154 82 568 438
Fragments arranged by size:
568 438 154 82
BmgBI CAC'GTC
Cuts at: 0 996 1242
Size: 996 246
BmrI ACTGGGnnnn_n'
Cuts at: 0 826 1242
Size: 826 416
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 244 454 1242
Size: 244 210 788
Fragments arranged by size:
788 244 210
Bpu10I CC'TnA_GC
Cuts at: 0 127 1006 1121 1242
Size: 127 879 115 121
Fragments arranged by size:
879 127 121 115
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 628 1242
Size: 628 614
BsaI GGTCTCn'nnnn_
Cuts at: 0 262 1242
Size: 262 980
BsaBI GATnn'nnATC
Cuts at: 0 508 1242
Size: 508 734
BsaHI Gr'CG_yC
Cuts at: 0 161 185 1242
Size: 161 24 1057
Fragments arranged by size:
1057 161 24
BsaWI w'CCGG_w
Cuts at: 0 338 1242
Size: 338 904
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 157 1224 1242
Size: 157 1067 18
Fragments arranged by size:
1067 157 18
BseYI C'CCAG_C
Cuts at: 0 1189 1242
Size: 1189 53
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 111 136 1242
Size: 111 25 1106
Fragments arranged by size:
1106 111 25
BsiEI CG_ry'CG
Cuts at: 0 136 140 769 1242
Size: 136 4 629 473
Fragments arranged by size:
629 473 136 4
BsiHKAI G_wGCw'C
Cuts at: 0 236 335 398 794 973 1242
Size: 236 99 63 396 179 269
Fragments arranged by size:
396 269 236 179 99 63
Bsp1286I G_dGCh'C
Cuts at: 0 154 236 335 398 794 804 973 1242
Size: 154 82 99 63 396 10 169 269
Fragments arranged by size:
396 269 169 154 99 82 63 10
BspEI T'CCGG_A
Cuts at: 0 338 1242
Size: 338 904
BsrBI CCG'CTC
Cuts at: 0 288 317 765 1056 1242
Size: 288 29 448 291 186
Fragments arranged by size:
448 291 288 186 29
BsrFI r'CCGG_y
Cuts at: 0 131 1032 1177 1242
Size: 131 901 145 65
Fragments arranged by size:
901 145 131 65
BsrGI T'GTAC_A
Cuts at: 0 590 1242
Size: 590 652
BssSI C'ACGA_G
Cuts at: 0 399 499 1242
Size: 399 100 743
Fragments arranged by size:
743 399 100
BstXI CCAn_nnnn'nTGG
Cuts at: 0 279 1242
Size: 279 963
BstYI r'GATC_y
Cuts at: 0 371 1242
Size: 371 871
BtgI C'CryG_G
Cuts at: 0 623 977 1242
Size: 623 354 265
Fragments arranged by size:
623 354 265
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 930 1242
Size: 930 312
ClaI AT'CG_AT
Cuts at: 0 254 1242
Size: 254 988
DraIII CAC_nnn'GTG
Cuts at: 0 702 1242
Size: 702 540
EaeI y'GGCC_r
Cuts at: 0 133 766 1025 1220 1242
Size: 133 633 259 195 22
Fragments arranged by size:
633 259 195 133 22
EagI C'GGCC_G
Cuts at: 0 133 766 1242
Size: 133 633 476
Fragments arranged by size:
633 476 133
EarI CTCTTCn'nnn_
Cuts at: 0 16 835 1242
Size: 16 819 407
Fragments arranged by size:
819 407 16
EcoICRI GAG'CTC
Cuts at: 0 333 396 792 1242
Size: 333 63 396 450
Fragments arranged by size:
450 396 333 63
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 34 244 454 1242
Size: 34 210 210 788
Fragments arranged by size:
788 210 210 34
EcoNI CCTnn'n_nnAGG
Cuts at: 0 592 808 1242
Size: 592 216 434
Fragments arranged by size:
592 434 216
EcoO109I rG'GnC_Cy
Cuts at: 0 150 151 528 635 801 1242
Size: 150 1 377 107 166 441
Fragments arranged by size:
441 377 166 150 107 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 462 1242
Size: 462 780
HaeII r_GCGC'y
Cuts at: 0 1070 1242
Size: 1070 172
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 202 234 661 693 706 738 1242
Size: 202 32 427 32 13 32 504
Fragments arranged by size:
504 427 202 32 32 32 13
HindIII A'AGCT_T
Cuts at: 0 1018 1242
Size: 1018 224
MscI TGG'CCA
Cuts at: 0 1222 1242
Size: 1222 20
MslI CAynn'nnrTG
Cuts at: 0 976 1242
Size: 976 266
MspA1I CmG'CkG
Cuts at: 0 120 1242
Size: 120 1122
NaeI GCC'GGC
Cuts at: 0 133 1034 1179 1242
Size: 133 901 145 63
Fragments arranged by size:
901 145 133 63
NcoI C'CATG_G
Cuts at: 0 977 1242
Size: 977 265
NgoMIV G'CCGG_C
Cuts at: 0 131 1032 1177 1242
Size: 131 901 145 65
Fragments arranged by size:
901 145 131 65
NspI r_CATG'y
Cuts at: 0 1064 1242
Size: 1064 178
PflMI CCAn_nnn'nTGG
Cuts at: 0 222 1242
Size: 222 1020
PfoI T'CCnGG_A
Cuts at: 0 242 278 785 1242
Size: 242 36 507 457
Fragments arranged by size:
507 457 242 36
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 742 774 1242
Size: 742 32 468
Fragments arranged by size:
742 468 32
PshAI GACnn'nnGTC
Cuts at: 0 184 274 1242
Size: 184 90 968
Fragments arranged by size:
968 184 90
PspOMI G'GGCC_C
Cuts at: 0 150 800 1242
Size: 150 650 442
Fragments arranged by size:
650 442 150
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 583 615 1242
Size: 583 32 627
Fragments arranged by size:
627 583 32
PstI C_TGCA'G
Cuts at: 0 962 1242
Size: 962 280
PvuI CG_AT'CG
Cuts at: 0 140 1242
Size: 140 1102
PvuII CAG'CTG
Cuts at: 0 120 1242
Size: 120 1122
SacI G_AGCT'C
Cuts at: 0 335 398 794 1242
Size: 335 63 396 448
Fragments arranged by size:
448 396 335 63
SbfI CC_TGCA'GG
Cuts at: 0 962 1242
Size: 962 280
SfcI C'TryA_G
Cuts at: 0 958 1242
Size: 958 284
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1219 1228 1242
Size: 1219 9 14
Fragments arranged by size:
1219 14 9
SgrAI Cr'CCGG_yG
Cuts at: 0 1177 1242
Size: 1177 65
SmaI CCC'GGG
Cuts at: 0 799 1242
Size: 799 443
SmlI C'TyrA_G
Cuts at: 0 643 1242
Size: 643 599
StuI AGG'CCT
Cuts at: 0 76 352 1231 1242
Size: 76 276 879 11
Fragments arranged by size:
879 276 76 11
StyI C'CwwG_G
Cuts at: 0 977 1226 1242
Size: 977 249 16
Fragments arranged by size:
977 249 16
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 714 1242
Size: 714 528
TatI w'GTAC_w
Cuts at: 0 248 590 1242
Size: 248 342 652
Fragments arranged by size:
652 342 248
Tth111I GACn'n_nGTC
Cuts at: 0 778 1242
Size: 778 464
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 429 993 1242
Size: 429 564 249
Fragments arranged by size:
564 429 249
XmaI C'CCGG_G
Cuts at: 0 797 1242
Size: 797 445
ZraI GAC'GTC
Cuts at: 0 162 186 1242
Size: 162 24 1056
Fragments arranged by size:
1056 162 24
Enzymes that do cut:
AatII AcuI AflIII AhdI AlwNI ApaI ApaLI ApoI
AvaI BanI BanII BbsI BbvCI BglI BlpI Bme1580I
BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BsrBI BsrFI BsrGI BssSI BstXI BstYI BtgI BtgZI
ClaI DraIII EaeI EagI EarI EcoICRI Eco57MI EcoNI
EcoO109I FalI HaeII Hin4I HindIII MscI MslI MspA1I
NaeI NcoI NgoMIV NspI PflMI PfoI PpiI PshAI
PspOMI PsrI PstI PvuI PvuII SacI SbfI SfcI
SfiI SgrAI SmaI SmlI StuI StyI TaqII TatI
Tth111I XcmI XmaI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AgeI AleI
AlfI AloI AscI AseI AsiSI AvrII BaeI BamHI
BbeI BcgI BciVI BclI BfrBI BglII BmtI BplI
BsaAI BsaXI BsiWI BsmI BsmBI BspHI BspMI BsrDI
BssHII BstAPI BstBI BstEII BstZ17I Bsu36I BtsI CspCI
DraI DrdI EciI EcoRI EcoRV FseI FspI FspAI
HincII HpaI KasI KpnI MfeI MluI MmeI NarI
NdeI NheI NotI NruI NsiI PacI PasI PciI
PmeI PmlI PpuMI PsiI PspXI RsrII SacII SalI
SanDI SapI ScaI SexAI SfoI SgfI SnaBI SpeI
SphI SrfI SspI SwaI TstI XbaI XhoI XmnI