Restriction map for
pFN21AE3651
(Linear) (Six-base) MAPSORT of: KIEE3651.seq Check: 8143 from: 1 to: 2694
KIEE3651.orf
With 182 enzymes: SgfI *
December 27, 2011 16:37 ..
AclI AA'CG_TT
Cuts at: 0 718 2694
Size: 718 1976
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 37 340 1465 1802 2542 2694
Size: 37 303 1125 337 740 152
Fragments arranged by size:
1125 740 337 303 152 37
AfeI AGC'GCT
Cuts at: 0 1300 2694
Size: 1300 1394
AflII C'TTAA_G
Cuts at: 0 208 2694
Size: 208 2486
AflIII A'CryG_T
Cuts at: 0 849 1994 2694
Size: 849 1145 700
Fragments arranged by size:
1145 849 700
AleI CACnn'nnGTG
Cuts at: 0 137 2694
Size: 137 2557
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2465 2497 2694
Size: 2465 32 197
Fragments arranged by size:
2465 197 32
AlwNI CAG_nnn'CTG
Cuts at: 0 325 1815 2694
Size: 325 1490 879
Fragments arranged by size:
1490 879 325
ApoI r'AATT_y
Cuts at: 0 220 2655 2694
Size: 220 2435 39
Fragments arranged by size:
2435 220 39
AvaI C'yCGr_G
Cuts at: 0 1967 2694
Size: 1967 727
BanI G'GyrC_C
Cuts at: 0 1403 2694
Size: 1403 1291
BanII G_rGCy'C
Cuts at: 0 2483 2694
Size: 2483 211
BbeI G_GCGC'C
Cuts at: 0 1407 2694
Size: 1407 1287
BbsI GAAGACnn'nnnn_
Cuts at: 0 992 2694
Size: 992 1702
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1873 1907 2694
Size: 1873 34 787
Fragments arranged by size:
1873 787 34
BclI T'GATC_A
Cuts at: 0 1859 2694
Size: 1859 835
BfrBI ATG'CAT
Cuts at: 0 424 2694
Size: 424 2270
BglI GCCn_nnn'nGGC
Cuts at: 0 2302 2694
Size: 2302 392
BglII A'GATC_T
Cuts at: 0 1607 2694
Size: 1607 1087
BlpI GC'TnA_GC
Cuts at: 0 318 2694
Size: 318 2376
Bme1580I G_kGCm'C
Cuts at: 0 541 2694
Size: 541 2153
BmrI ACTGGGnnnn_n'
Cuts at: 0 1251 2694
Size: 1251 1443
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1761 2522 2694
Size: 1761 761 172
Fragments arranged by size:
1761 761 172
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 542 1453 1543 2080 2389 2467 2694
Size: 542 911 90 537 309 78 227
Fragments arranged by size:
911 542 537 309 227 90 78
Bpu10I CC'TnA_GC
Cuts at: 0 312 1241 2694
Size: 312 929 1453
Fragments arranged by size:
1453 929 312
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1456 2194 2330 2354 2412 2545 2694
Size: 1456 738 136 24 58 133 149
Fragments arranged by size:
1456 738 149 136 133 58 24
BsaI GGTCTCn'nnnn_
Cuts at: 0 830 2694
Size: 830 1864
BsaAI yAC'GTr
Cuts at: 0 850 2694
Size: 850 1844
BsaHI Gr'CG_yC
Cuts at: 0 1404 2694
Size: 1404 1290
BsaWI w'CCGG_w
Cuts at: 0 1286 2694
Size: 1286 1408
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1351 1381 2465 2495 2694
Size: 1351 30 1084 30 199
Fragments arranged by size:
1351 1084 199 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1164 2557 2694
Size: 1164 1393 137
Fragments arranged by size:
1393 1164 137
BseYI C'CCAG_C
Cuts at: 0 1616 2694
Size: 1616 1078
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1975 2074 2694
Size: 1975 99 620
Fragments arranged by size:
1975 620 99
BsiHKAI G_wGCw'C
Cuts at: 0 1069 1466 2249 2483 2520 2694
Size: 1069 397 783 234 37 174
Fragments arranged by size:
1069 783 397 234 174 37
BsmI GAATG_Cn'
Cuts at: 0 678 2386 2694
Size: 678 1708 308
Fragments arranged by size:
1708 678 308
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1486 2694
Size: 1486 1208
Bsp1286I G_dGCh'C
Cuts at: 0 541 1069 1466 2249 2483 2520 2694
Size: 541 528 397 783 234 37 174
Fragments arranged by size:
783 541 528 397 234 174 37
BspHI T'CATG_A
Cuts at: 0 1136 2694
Size: 1136 1558
BsrBI CCG'CTC
Cuts at: 0 1740 2694
Size: 1740 954
BsrDI GCAATG_nn'
Cuts at: 0 683 695 2694
Size: 683 12 1999
Fragments arranged by size:
1999 683 12
BsrFI r'CCGG_y
Cuts at: 0 839 2694
Size: 839 1855
BssSI C'ACGA_G
Cuts at: 0 1870 2694
Size: 1870 824
BstAPI GCAn_nnn'nTGC
Cuts at: 0 682 1941 2694
Size: 682 1259 753
Fragments arranged by size:
1259 753 682
BstXI CCAn_nnnn'nTGG
Cuts at: 0 144 1521 2694
Size: 144 1377 1173
Fragments arranged by size:
1377 1173 144
BstYI r'GATC_y
Cuts at: 0 499 1340 1607 1742 2016 2694
Size: 499 841 267 135 274 678
Fragments arranged by size:
841 678 499 274 267 135
Bsu36I CC'TnA_GG
Cuts at: 0 59 785 2678 2694
Size: 59 726 1893 16
Fragments arranged by size:
1893 726 59 16
BtgI C'CryG_G
Cuts at: 0 1224 1246 2141 2694
Size: 1224 22 895 553
Fragments arranged by size:
1224 895 553 22
BtsI GCAGTG_nn'
Cuts at: 0 284 1092 2694
Size: 284 808 1602
Fragments arranged by size:
1602 808 284
DraIII CAC_nnn'GTG
Cuts at: 0 850 1875 2694
Size: 850 1025 819
Fragments arranged by size:
1025 850 819
EaeI y'GGCC_r
Cuts at: 0 841 1532 2694
Size: 841 691 1162
Fragments arranged by size:
1162 841 691
EarI CTCTTCn'nnn_
Cuts at: 0 1778 2534 2694
Size: 1778 756 160
Fragments arranged by size:
1778 756 160
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 658 2694
Size: 658 2036
EcoICRI GAG'CTC
Cuts at: 0 2481 2694
Size: 2481 213
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 37 340 542 1453 1465 1543 1802 2080
Size: 37 303 202 911 12 78 259 278
Cuts at: 2080 2389 2467 2542 2694
Size: 309 78 75 152
Fragments arranged by size:
911 309 303 278 259 202 152 78
78 75 37 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2560 2694
Size: 2560 134
EcoO109I rG'GnC_Cy
Cuts at: 0 21 1275 1916 2694
Size: 21 1254 641 778
Fragments arranged by size:
1254 778 641 21
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 223 1813 2694
Size: 223 1590 881
Fragments arranged by size:
1590 881 223
FspI TGC'GCA
Cuts at: 0 2512 2694
Size: 2512 182
HaeII r_GCGC'y
Cuts at: 0 1302 1407 2694
Size: 1302 105 1287
Fragments arranged by size:
1302 1287 105
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 21 53 484 516 598 630 2328 2360
Size: 21 32 431 32 82 32 1698 32
Cuts at: 2360 2694
Size: 334
Fragments arranged by size:
1698 431 334 82 32 32 32 32
21
KasI G'GCGC_C
Cuts at: 0 1403 2694
Size: 1403 1291
MfeI C'AATT_G
Cuts at: 0 232 2694
Size: 232 2462
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2204 2694
Size: 2204 490
MscI TGG'CCA
Cuts at: 0 1534 2694
Size: 1534 1160
MslI CAynn'nnrTG
Cuts at: 0 137 419 1060 1349 2146 2474 2694
Size: 137 282 641 289 797 328 220
Fragments arranged by size:
797 641 328 289 282 220 137
MspA1I CmG'CkG
Cuts at: 0 1248 1422 1989 2058 2694
Size: 1248 174 567 69 636
Fragments arranged by size:
1248 636 567 174 69
NarI GG'CG_CC
Cuts at: 0 1404 2694
Size: 1404 1290
NcoI C'CATG_G
Cuts at: 0 2141 2694
Size: 2141 553
NsiI A_TGCA'T
Cuts at: 0 426 2694
Size: 426 2268
NspI r_CATG'y
Cuts at: 0 1059 1101 1501 1939 2104 2694
Size: 1059 42 400 438 165 590
Fragments arranged by size:
1059 590 438 400 165 42
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1579 1611 2465 2497 2694
Size: 1579 32 854 32 197
Fragments arranged by size:
1579 854 197 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 21 1916 2694
Size: 21 1895 778
Fragments arranged by size:
1895 778 21
PsiI TTA'TAA
Cuts at: 0 1391 2694
Size: 1391 1303
PstI C_TGCA'G
Cuts at: 0 1581 2694
Size: 1581 1113
PvuII CAG'CTG
Cuts at: 0 1422 1989 2058 2694
Size: 1422 567 69 636
Fragments arranged by size:
1422 636 567 69
SacI G_AGCT'C
Cuts at: 0 2483 2694
Size: 2483 211
SacII CC_GC'GG
Cuts at: 0 1249 2694
Size: 1249 1445
SfcI C'TryA_G
Cuts at: 0 168 907 1477 1577 2398 2688 2694
Size: 168 739 570 100 821 290 6
Fragments arranged by size:
821 739 570 290 168 100 6
SfoI GGC'GCC
Cuts at: 0 1405 2694
Size: 1405 1289
SmlI C'TyrA_G
Cuts at: 0 208 1471 2209 2345 2369 2391 2524 2694
Size: 208 1263 738 136 24 22 133 170
Fragments arranged by size:
1263 738 208 170 136 133 24 22
SphI G_CATG'C
Cuts at: 0 1101 1501 1939 2694
Size: 1101 400 438 755
Fragments arranged by size:
1101 755 438 400
SspI AAT'ATT
Cuts at: 0 307 456 2694
Size: 307 149 2238
Fragments arranged by size:
2238 307 149
StuI AGG'CCT
Cuts at: 0 1239 2389 2694
Size: 1239 1150 305
Fragments arranged by size:
1239 1150 305
StyI C'CwwG_G
Cuts at: 0 2141 2694
Size: 2141 553
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1734 2694
Size: 1734 960
TatI w'GTAC_w
Cuts at: 0 257 1817 1979 2063 2184 2694
Size: 257 1560 162 84 121 510
Fragments arranged by size:
1560 510 257 162 121 84
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 836 868 1325 1357 1945 1977 2694
Size: 836 32 457 32 588 32 717
Fragments arranged by size:
836 717 588 457 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 144 531 1542 2694
Size: 144 387 1011 1152
Fragments arranged by size:
1152 1011 387 144
XmnI GAAnn'nnTTC
Cuts at: 0 1594 2694
Size: 1594 1100
Enzymes that do cut:
AclI AcuI AfeI AflII AflIII AleI AloI AlwNI
ApoI AvaI BanI BanII BbeI BbsI BcgI BclI
BfrBI BglI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspHI BsrBI
BsrDI BsrFI BssSI BstAPI BstXI BstYI Bsu36I BtgI
BtsI DraIII EaeI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I FalI FspI HaeII Hin4I KasI MfeI MmeI
MscI MslI MspA1I NarI NcoI NsiI NspI PpiI
PpuMI PsiI PstI PvuII SacI SacII SfcI SfoI
SmlI SphI SspI StuI StyI TaqII TatI TstI
XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AgeI AhdI AlfI ApaI
ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbvCI
BciVI BmgBI BmtI BsaBI BsiEI BsiWI BspEI BspMI
BsrGI BssHII BstBI BstEII BstZ17I BtgZI ClaI CspCI
DraI DrdI EagI EcoRI EcoRV FseI FspAI HincII
HindIII HpaI KpnI MluI NaeI NdeI NgoMIV NheI
NotI NruI PacI PasI PciI PflMI PfoI PmeI
PmlI PshAI PspOMI PspXI PsrI PvuI RsrII SalI
SanDI SapI SbfI ScaI SexAI SfiI SgfI SgrAI
SmaI SnaBI SpeI SrfI SwaI Tth111I XbaI XhoI
XmaI ZraI