Restriction map for
pFN21AE3648
(Linear) (Six-base) MAPSORT of: KIEE3648.seq Check: 1770 from: 1 to: 702
KIEE3648.orf
With 182 enzymes: SgfI *
December 27, 2011 16:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 432 702
Size: 432 270
AatII G_ACGT'C
Cuts at: 0 58 702
Size: 58 644
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 148 702
Size: 148 554
AgeI A'CCGG_T
Cuts at: 0 278 702
Size: 278 424
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 40 702
Size: 40 662
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 265 297 702
Size: 265 32 405
Fragments arranged by size:
405 265 32
AlwNI CAG_nnn'CTG
Cuts at: 0 261 702
Size: 261 441
BanI G'GyrC_C
Cuts at: 0 115 432 603 702
Size: 115 317 171 99
Fragments arranged by size:
317 171 115 99
BanII G_rGCy'C
Cuts at: 0 199 702
Size: 199 503
BbeI G_GCGC'C
Cuts at: 0 119 702
Size: 119 583
BbsI GAAGACnn'nnnn_
Cuts at: 0 58 702
Size: 58 644
BglII A'GATC_T
Cuts at: 0 685 702
Size: 685 17
BlpI GC'TnA_GC
Cuts at: 0 524 702
Size: 524 178
Bme1580I G_kGCm'C
Cuts at: 0 435 702
Size: 435 267
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 292 472 702
Size: 292 180 230
Fragments arranged by size:
292 230 180
Bpu10I CC'TnA_GC
Cuts at: 0 437 702
Size: 437 265
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 469 702
Size: 469 233
BsaI GGTCTCn'nnnn_
Cuts at: 0 535 577 702
Size: 535 42 125
Fragments arranged by size:
535 125 42
BsaBI GATnn'nnATC
Cuts at: 0 245 702
Size: 245 457
BsaHI Gr'CG_yC
Cuts at: 0 55 116 702
Size: 55 61 586
Fragments arranged by size:
586 61 55
BsaWI w'CCGG_w
Cuts at: 0 278 702
Size: 278 424
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 136 166 265 295 406 436 702
Size: 136 30 99 30 111 30 266
Fragments arranged by size:
266 136 111 99 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 370 702
Size: 370 332
BseYI C'CCAG_C
Cuts at: 0 409 702
Size: 409 293
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 411 466 702
Size: 411 55 236
Fragments arranged by size:
411 236 55
BsiEI CG_ry'CG
Cuts at: 0 221 521 702
Size: 221 300 181
Fragments arranged by size:
300 221 181
BsiHKAI G_wGCw'C
Cuts at: 0 29 702
Size: 29 673
Bsp1286I G_dGCh'C
Cuts at: 0 29 199 435 702
Size: 29 170 236 267
Fragments arranged by size:
267 236 170 29
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 432 702
Size: 432 270
BsrBI CCG'CTC
Cuts at: 0 81 702
Size: 81 621
BsrFI r'CCGG_y
Cuts at: 0 251 278 516 556 702
Size: 251 27 238 40 146
Fragments arranged by size:
251 238 146 40 27
BstAPI GCAn_nnn'nTGC
Cuts at: 0 314 513 702
Size: 314 199 189
Fragments arranged by size:
314 199 189
BstYI r'GATC_y
Cuts at: 0 479 685 702
Size: 479 206 17
Fragments arranged by size:
479 206 17
BtgI C'CryG_G
Cuts at: 0 186 702
Size: 186 516
BtsI GCAGTG_nn'
Cuts at: 0 139 702
Size: 139 563
DraIII CAC_nnn'GTG
Cuts at: 0 648 702
Size: 648 54
EaeI y'GGCC_r
Cuts at: 0 218 518 702
Size: 218 300 184
Fragments arranged by size:
300 218 184
EagI C'GGCC_G
Cuts at: 0 218 518 702
Size: 218 300 184
Fragments arranged by size:
300 218 184
EarI CTCTTCn'nnn_
Cuts at: 0 325 380 702
Size: 325 55 322
Fragments arranged by size:
325 322 55
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 148 292 472 702
Size: 148 144 180 230
Fragments arranged by size:
230 180 148 144
EcoNI CCTnn'n_nnAGG
Cuts at: 0 352 702
Size: 352 350
EcoO109I rG'GnC_Cy
Cuts at: 0 362 702
Size: 362 340
HaeII r_GCGC'y
Cuts at: 0 87 119 141 551 702
Size: 87 32 22 410 151
Fragments arranged by size:
410 151 87 32 22
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 406 438 622 654 702
Size: 406 32 184 32 48
Fragments arranged by size:
406 184 48 32 32
KasI G'GCGC_C
Cuts at: 0 115 702
Size: 115 587
MslI CAynn'nnrTG
Cuts at: 0 43 702
Size: 43 659
MspA1I CmG'CkG
Cuts at: 0 69 450 523 702
Size: 69 381 73 179
Fragments arranged by size:
381 179 73 69
NaeI GCC'GGC
Cuts at: 0 518 702
Size: 518 184
NarI GG'CG_CC
Cuts at: 0 116 702
Size: 116 586
NgoMIV G'CCGG_C
Cuts at: 0 516 702
Size: 516 186
NotI GC'GGCC_GC
Cuts at: 0 218 702
Size: 218 484
PflMI CCAn_nnn'nTGG
Cuts at: 0 192 702
Size: 192 510
PfoI T'CCnGG_A
Cuts at: 0 89 702
Size: 89 613
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 265 297 702
Size: 265 32 405
Fragments arranged by size:
405 265 32
PsiI TTA'TAA
Cuts at: 0 473 702
Size: 473 229
PstI C_TGCA'G
Cuts at: 0 68 702
Size: 68 634
PvuII CAG'CTG
Cuts at: 0 450 702
Size: 450 252
SexAI A'CCwGG_T
Cuts at: 0 596 702
Size: 596 106
SfcI C'TryA_G
Cuts at: 0 64 702
Size: 64 638
SfoI GGC'GCC
Cuts at: 0 117 702
Size: 117 585
SmlI C'TyrA_G
Cuts at: 0 484 702
Size: 484 218
StyI C'CwwG_G
Cuts at: 0 365 427 702
Size: 365 62 275
Fragments arranged by size:
365 275 62
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 680 687 702
Size: 680 7 15
Fragments arranged by size:
680 15 7
TatI w'GTAC_w
Cuts at: 0 203 488 702
Size: 203 285 214
Fragments arranged by size:
285 214 203
Tth111I GACn'n_nGTC
Cuts at: 0 97 418 702
Size: 97 321 284
Fragments arranged by size:
321 284 97
ZraI GAC'GTC
Cuts at: 0 56 702
Size: 56 646
Enzymes that do cut:
AarI AatII AcuI AgeI AlfI AloI AlwNI BanI
BanII BbeI BbsI BglII BlpI Bme1580I BpmI Bpu10I
BpuEI BsaI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrFI BstAPI
BstYI BtgI BtsI DraIII EaeI EagI EarI Eco57MI
EcoNI EcoO109I HaeII Hin4I KasI MslI MspA1I NaeI
NarI NgoMIV NotI PflMI PfoI PpiI PsiI PstI
PvuII SexAI SfcI SfoI SmlI StyI TaqII TatI
Tth111I ZraI
Enzymes that do not cut:
AccI Acc65I AclI AfeI AflII AflIII AhdI AleI
ApaI ApaLI ApoI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BbvCI BcgI BciVI BclI BfrBI BglI
BmgBI BmrI BmtI BplI BsaAI BsiWI BsmI BsmBI
BspEI BspHI BsrDI BsrGI BssHII BssSI BstBI BstEII
BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DrdI
EciI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
NcoI NdeI NheI NruI NsiI NspI PacI PasI
PciI PmeI PmlI PpuMI PshAI PspOMI PspXI PsrI
PvuI RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI StuI SwaI TstI XbaI XcmI XhoI
XmaI XmnI