(Linear) (Six-base) MAPSORT of: KIEE3645.seq  Check: 8657  from: 1  to: 222



KIEE3645.orf

 With 182 enzymes: SgfI * 

                        December 27, 2011 16:37  ..

BamHI G'GATC_C

Cuts at:      0     150     222 
   Size:        150      72
 
BanII G_rGCy'C

Cuts at:      0     200     222 
   Size:        200      22
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      49     222 
   Size:         49     173
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     197     222 
   Size:        197      25
 
BsiHKAI G_wGCw'C

Cuts at:      0     200     222 
   Size:        200      22
 
BsmI GAATG_Cn'

Cuts at:      0      95     222 
   Size:         95     127
 
Bsp1286I G_dGCh'C

Cuts at:      0     200     222 
   Size:        200      22
 
BstYI r'GATC_y

Cuts at:      0     150     222 
   Size:        150      72
 
EcoICRI GAG'CTC

Cuts at:      0     198     222 
   Size:        198      24
 
EcoO109I rG'GnC_Cy

Cuts at:      0      21     222 
   Size:         21     201
 
HincII GTy'rAC

Cuts at:      0      57     222 
   Size:         57     165
 
PpuMI rG'GwC_Cy

Cuts at:      0      21     222 
   Size:         21     201
 
SacI G_AGCT'C

Cuts at:      0     200     222 
   Size:        200      22
 
SmlI C'TyrA_G

Cuts at:      0      64     222 
   Size:         64     158
 
SspI AAT'ATT

Cuts at:      0      15     222 
   Size:         15     207
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     197     222 
   Size:        197      25
 

 Enzymes that do cut:

    BamHI    BanII    BpuEI    BsaXI  BsiHKAI     BsmI Bsp1286I    BstYI
  EcoICRI EcoO109I   HincII    PpuMI     SacI     SmlI     SspI     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI
     BanI     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI
     BpmI   Bpu10I     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BseRI
    BseYI     BsgI    BsiEI    BsiWI    BsmBI    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI
    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EarI     EciI
  Eco57MI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII    Hin4I  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     StuI     StyI     SwaI    TaqII     TatI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI