Restriction map for
pFN21AE3644
(Linear) (Six-base) MAPSORT of: KIEE3644.seq Check: 3547 from: 1 to: 1602
KIEE3644.orf
With 182 enzymes: SgfI *
December 27, 2011 16:37 ..
AclI AA'CG_TT
Cuts at: 0 1031 1602
Size: 1031 571
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 278 711 1217 1378 1568 1602
Size: 278 433 506 161 190 34
Fragments arranged by size:
506 433 278 190 161 34
AlwNI CAG_nnn'CTG
Cuts at: 0 127 1602
Size: 127 1475
ApoI r'AATT_y
Cuts at: 0 131 271 432 749 789 937 963 1441
Size: 131 140 161 317 40 148 26 478
Cuts at: 1441 1602
Size: 161
Fragments arranged by size:
478 317 161 161 148 140 131 40
26
BanI G'GyrC_C
Cuts at: 0 1110 1602
Size: 1110 492
BanII G_rGCy'C
Cuts at: 0 774 1602
Size: 774 828
BbvCI CC'TCA_GC
Cuts at: 0 49 1602
Size: 49 1553
BciVI GTATCCnnnnn_n'
Cuts at: 0 1206 1602
Size: 1206 396
BclI T'GATC_A
Cuts at: 0 63 647 1602
Size: 63 584 955
Fragments arranged by size:
955 584 63
BfrBI ATG'CAT
Cuts at: 0 535 1602
Size: 535 1067
BlpI GC'TnA_GC
Cuts at: 0 414 1463 1602
Size: 414 1049 139
Fragments arranged by size:
1049 414 139
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 758 1602
Size: 758 844
Bpu10I CC'TnA_GC
Cuts at: 0 49 1191 1602
Size: 49 1142 411
Fragments arranged by size:
1142 411 49
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 7 1490 1602
Size: 7 1483 112
Fragments arranged by size:
1483 112 7
BsaBI GATnn'nnATC
Cuts at: 0 185 602 646 1185 1602
Size: 185 417 44 539 417
Fragments arranged by size:
539 417 417 185 44
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 19 49 1602
Size: 19 30 1553
Fragments arranged by size:
1553 30 19
BseYI C'CCAG_C
Cuts at: 0 9 1602
Size: 9 1593
BsiEI CG_ry'CG
Cuts at: 0 42 1602
Size: 42 1560
BsmI GAATG_Cn'
Cuts at: 0 1483 1602
Size: 1483 119
Bsp1286I G_dGCh'C
Cuts at: 0 774 1602
Size: 774 828
BsrDI GCAATG_nn'
Cuts at: 0 1475 1602
Size: 1475 127
BsrFI r'CCGG_y
Cuts at: 0 41 1602
Size: 41 1561
BssSI C'ACGA_G
Cuts at: 0 923 1602
Size: 923 679
BstXI CCAn_nnnn'nTGG
Cuts at: 0 911 1602
Size: 911 691
BstYI r'GATC_y
Cuts at: 0 543 839 1602
Size: 543 296 763
Fragments arranged by size:
763 543 296
BtgI C'CryG_G
Cuts at: 0 266 637 1602
Size: 266 371 965
Fragments arranged by size:
965 371 266
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 308 1602
Size: 308 1294
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1426 1461 1602
Size: 1426 35 141
Fragments arranged by size:
1426 141 35
EaeI y'GGCC_r
Cuts at: 0 944 1104 1602
Size: 944 160 498
Fragments arranged by size:
944 498 160
EarI CTCTTCn'nnn_
Cuts at: 0 311 779 1602
Size: 311 468 823
Fragments arranged by size:
823 468 311
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 278 711 758 1217 1378 1568 1602
Size: 278 433 47 459 161 190 34
Fragments arranged by size:
459 433 278 190 161 47 34
EcoNI CCTnn'n_nnAGG
Cuts at: 0 974 1602
Size: 974 628
EcoRI G'AATT_C
Cuts at: 0 271 432 749 1602
Size: 271 161 317 853
Fragments arranged by size:
853 317 271 161
EcoRV GAT'ATC
Cuts at: 0 1324 1602
Size: 1324 278
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 906 938 1602
Size: 906 32 664
Fragments arranged by size:
906 664 32
MscI TGG'CCA
Cuts at: 0 946 1106 1602
Size: 946 160 496
Fragments arranged by size:
946 496 160
MspA1I CmG'CkG
Cuts at: 0 9 48 197 1066 1602
Size: 9 39 149 869 536
Fragments arranged by size:
869 536 149 39 9
NcoI C'CATG_G
Cuts at: 0 266 637 1602
Size: 266 371 965
Fragments arranged by size:
965 371 266
NdeI CA'TA_TG
Cuts at: 0 465 608 1602
Size: 465 143 994
Fragments arranged by size:
994 465 143
NsiI A_TGCA'T
Cuts at: 0 537 1602
Size: 537 1065
NspI r_CATG'y
Cuts at: 0 1575 1602
Size: 1575 27
PflMI CCAn_nnn'nTGG
Cuts at: 0 160 1602
Size: 160 1442
PfoI T'CCnGG_A
Cuts at: 0 1326 1602
Size: 1326 276
PshAI GACnn'nnGTC
Cuts at: 0 626 1602
Size: 626 976
PsiI TTA'TAA
Cuts at: 0 496 1602
Size: 496 1106
PstI C_TGCA'G
Cuts at: 0 1097 1153 1602
Size: 1097 56 449
Fragments arranged by size:
1097 449 56
PvuII CAG'CTG
Cuts at: 0 9 48 1602
Size: 9 39 1554
Fragments arranged by size:
1554 39 9
SexAI A'CCwGG_T
Cuts at: 0 153 1602
Size: 153 1449
SfcI C'TryA_G
Cuts at: 0 360 456 1093 1149 1602
Size: 360 96 637 56 453
Fragments arranged by size:
637 453 360 96 56
SmlI C'TyrA_G
Cuts at: 0 22 1505 1602
Size: 22 1483 97
Fragments arranged by size:
1483 97 22
SpeI A'CTAG_T
Cuts at: 0 471 1602
Size: 471 1131
SspI AAT'ATT
Cuts at: 0 661 1602
Size: 661 941
StyI C'CwwG_G
Cuts at: 0 266 637 1602
Size: 266 371 965
Fragments arranged by size:
965 371 266
TatI w'GTAC_w
Cuts at: 0 1274 1602
Size: 1274 328
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 296 328 1602
Size: 296 32 1274
Fragments arranged by size:
1274 296 32
XbaI T'CTAG_A
Cuts at: 0 336 1602
Size: 336 1266
XmnI GAAnn'nnTTC
Cuts at: 0 332 584 1602
Size: 332 252 1018
Fragments arranged by size:
1018 332 252
Enzymes that do cut:
AclI AcuI AlwNI ApoI BanI BanII BbvCI BciVI
BclI BfrBI BlpI BpmI Bpu10I BpuEI BsaBI BsaXI
BseYI BsiEI BsmI Bsp1286I BsrDI BsrFI BssSI BstXI
BstYI BtgI BtgZI CspCI EaeI EarI Eco57MI EcoNI
EcoRI EcoRV Hin4I MscI MspA1I NcoI NdeI NsiI
NspI PflMI PfoI PshAI PsiI PstI PvuII SexAI
SfcI SmlI SpeI SspI StyI TatI TstI XbaI
XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BbeI BbsI BcgI
BglI BglII Bme1580I BmgBI BmrI BmtI BplI BsaI
BsaAI BsaHI BsaWI BseRI BsgI BsiHKAI BsiWI BsmBI
BspEI BspHI BspMI BsrBI BsrGI BssHII BstAPI BstBI
BstEII BstZ17I Bsu36I BtsI ClaI DraI DraIII DrdI
EagI EciI EcoICRI EcoO109I FalI FseI FspI FspAI
HaeII HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MslI NaeI NarI NgoMIV NheI NotI NruI
PacI PasI PciI PmeI PmlI PpiI PpuMI PspOMI
PspXI PsrI PvuI RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI
SnaBI SphI SrfI StuI SwaI TaqII Tth111I XcmI
XhoI XmaI ZraI