Restriction map for
pFN21AE3639
(Linear) (Six-base) MAPSORT of: KIEE3639.seq Check: 2056 from: 1 to: 1137
KIEE3639.orf
With 182 enzymes: SgfI *
December 27, 2011 16:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 754 1137
Size: 754 383
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 497 1137
Size: 497 640
AflIII A'CryG_T
Cuts at: 0 526 1137
Size: 526 611
ApaLI G'TGCA_C
Cuts at: 0 944 1137
Size: 944 193
ApoI r'AATT_y
Cuts at: 0 261 304 471 1137
Size: 261 43 167 666
Fragments arranged by size:
666 261 167 43
BamHI G'GATC_C
Cuts at: 0 1110 1137
Size: 1110 27
BanI G'GyrC_C
Cuts at: 0 189 554 1057 1137
Size: 189 365 503 80
Fragments arranged by size:
503 365 189 80
BbeI G_GCGC'C
Cuts at: 0 1061 1137
Size: 1061 76
BbsI GAAGACnn'nnnn_
Cuts at: 0 1008 1137
Size: 1008 129
BfrBI ATG'CAT
Cuts at: 0 863 1137
Size: 863 274
Bme1580I G_kGCm'C
Cuts at: 0 547 948 1137
Size: 547 401 189
Fragments arranged by size:
547 401 189
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 63 779 1137
Size: 63 716 358
Fragments arranged by size:
716 358 63
Bpu10I CC'TnA_GC
Cuts at: 0 1066 1137
Size: 1066 71
BsaI GGTCTCn'nnnn_
Cuts at: 0 899 1137
Size: 899 238
BsaHI Gr'CG_yC
Cuts at: 0 1058 1137
Size: 1058 79
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 91 121 1137
Size: 91 30 1016
Fragments arranged by size:
1016 91 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 24 112 1114 1137
Size: 24 88 1002 23
Fragments arranged by size:
1002 88 24 23
BseYI C'CCAG_C
Cuts at: 0 369 1061 1137
Size: 369 692 76
Fragments arranged by size:
692 369 76
BsiHKAI G_wGCw'C
Cuts at: 0 948 1137
Size: 948 189
BsmI GAATG_Cn'
Cuts at: 0 233 1137
Size: 233 904
Bsp1286I G_dGCh'C
Cuts at: 0 547 948 1137
Size: 547 401 189
Fragments arranged by size:
547 401 189
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 754 964 1137
Size: 754 210 173
Fragments arranged by size:
754 210 173
BsrBI CCG'CTC
Cuts at: 0 1104 1137
Size: 1104 33
BstEII G'GTnAC_C
Cuts at: 0 895 1137
Size: 895 242
BstYI r'GATC_y
Cuts at: 0 345 1110 1137
Size: 345 765 27
Fragments arranged by size:
765 345 27
Bsu36I CC'TnA_GG
Cuts at: 0 1045 1137
Size: 1045 92
BtgI C'CryG_G
Cuts at: 0 612 1137
Size: 612 525
BtsI GCAGTG_nn'
Cuts at: 0 67 820 1005 1137
Size: 67 753 185 132
Fragments arranged by size:
753 185 132 67
ClaI AT'CG_AT
Cuts at: 0 119 1137
Size: 119 1018
DraI TTT'AAA
Cuts at: 0 522 1137
Size: 522 615
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 91 1137
Size: 91 1046
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 63 497 779 1137
Size: 63 434 282 358
Fragments arranged by size:
434 358 282 63
EcoO109I rG'GnC_Cy
Cuts at: 0 761 1137
Size: 761 376
EcoRI G'AATT_C
Cuts at: 0 304 471 1137
Size: 304 167 666
Fragments arranged by size:
666 304 167
EcoRV GAT'ATC
Cuts at: 0 162 1137
Size: 162 975
HaeII r_GCGC'y
Cuts at: 0 17 1061 1137
Size: 17 1044 76
Fragments arranged by size:
1044 76 17
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 108 140 1137
Size: 108 32 997
Fragments arranged by size:
997 108 32
HincII GTy'rAC
Cuts at: 0 532 1137
Size: 532 605
KasI G'GCGC_C
Cuts at: 0 1057 1137
Size: 1057 80
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 373 1137
Size: 373 764
NarI GG'CG_CC
Cuts at: 0 1058 1137
Size: 1058 79
NsiI A_TGCA'T
Cuts at: 0 865 1137
Size: 865 272
NspI r_CATG'y
Cuts at: 0 530 1042 1137
Size: 530 512 95
Fragments arranged by size:
530 512 95
PciI A'CATG_T
Cuts at: 0 526 1137
Size: 526 611
PflMI CCAn_nnn'nTGG
Cuts at: 0 421 1137
Size: 421 716
PfoI T'CCnGG_A
Cuts at: 0 795 1137
Size: 795 342
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 366 398 1137
Size: 366 32 739
Fragments arranged by size:
739 366 32
PpuMI rG'GwC_Cy
Cuts at: 0 761 1137
Size: 761 376
PstI C_TGCA'G
Cuts at: 0 282 1137
Size: 282 855
ScaI AGT'ACT
Cuts at: 0 140 1137
Size: 140 997
SfcI C'TryA_G
Cuts at: 0 278 1137
Size: 278 859
SfoI GGC'GCC
Cuts at: 0 1059 1137
Size: 1059 78
TatI w'GTAC_w
Cuts at: 0 138 1137
Size: 138 999
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 656 688 1137
Size: 656 32 449
Fragments arranged by size:
656 449 32
Enzymes that do cut:
AarI AcuI AflIII ApaLI ApoI BamHI BanI BbeI
BbsI BfrBI Bme1580I BpmI Bpu10I BsaI BsaHI BsaXI
BseRI BseYI BsiHKAI BsmI Bsp1286I BspMI BsrBI BstEII
BstYI Bsu36I BtgI BtsI ClaI DraI EciI Eco57MI
EcoO109I EcoRI EcoRV HaeII Hin4I HincII KasI MmeI
NarI NsiI NspI PciI PflMI PfoI PpiI PpuMI
PstI ScaI SfcI SfoI TatI TstI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI AlwNI ApaI AscI AseI AsiSI
AvaI AvrII BaeI BanII BbvCI BcgI BciVI BclI
BglI BglII BlpI BmgBI BmrI BmtI BplI BpuEI
BsaAI BsaBI BsaWI BsgI BsiEI BsiWI BsmBI BspEI
BspHI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstXI BstZ17I BtgZI CspCI DraIII DrdI EaeI EagI
EarI EcoICRI EcoNI FalI FseI FspI FspAI HindIII
HpaI KpnI MfeI MluI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NruI PacI PasI
PmeI PmlI PshAI PsiI PspOMI PspXI PsrI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
SexAI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI StyI SwaI TaqII Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI