Restriction map for
pFN21AE3633
(Linear) (Six-base) MAPSORT of: KIEE3633.seq Check: 8751 from: 1 to: 2466
KIEE3633.orf
With 182 enzymes: SgfI *
March 22, 2012 16:38 ..
AccI GT'mk_AC
Cuts at: 0 1772 2426 2441 2466
Size: 1772 654 15 25
Fragments arranged by size:
1772 654 25 15
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1017 1581 1654 1702 2466
Size: 1017 564 73 48 764
Fragments arranged by size:
1017 764 564 73 48
AfeI AGC'GCT
Cuts at: 0 847 2466
Size: 847 1619
AflIII A'CryG_T
Cuts at: 0 1175 1177 2466
Size: 1175 2 1289
Fragments arranged by size:
1289 1175 2
AleI CACnn'nnGTG
Cuts at: 0 1667 2466
Size: 1667 799
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 976 2466
Size: 976 1490
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 205 237 2466
Size: 205 32 2229
Fragments arranged by size:
2229 205 32
AlwNI CAG_nnn'CTG
Cuts at: 0 444 525 708 1119 2382 2466
Size: 444 81 183 411 1263 84
Fragments arranged by size:
1263 444 411 183 84 81
AvaI C'yCGr_G
Cuts at: 0 806 943 1603 1834 2466
Size: 806 137 660 231 632
Fragments arranged by size:
806 660 632 231 137
BamHI G'GATC_C
Cuts at: 0 366 2466
Size: 366 2100
BanI G'GyrC_C
Cuts at: 0 93 2131 2466
Size: 93 2038 335
Fragments arranged by size:
2038 335 93
BanII G_rGCy'C
Cuts at: 0 164 314 655 1031 1480 2126 2466
Size: 164 150 341 376 449 646 340
Fragments arranged by size:
646 449 376 341 340 164 150
BbsI GAAGACnn'nnnn_
Cuts at: 0 113 2466
Size: 113 2353
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 283 317 2466
Size: 283 34 2149
Fragments arranged by size:
2149 283 34
BglI GCCn_nnn'nGGC
Cuts at: 0 1213 1858 2227 2466
Size: 1213 645 369 239
Fragments arranged by size:
1213 645 369 239
BlpI GC'TnA_GC
Cuts at: 0 727 2466
Size: 727 1739
Bme1580I G_kGCm'C
Cuts at: 0 430 583 2466
Size: 430 153 1883
Fragments arranged by size:
1883 430 153
BmgBI CAC'GTC
Cuts at: 0 1941 2466
Size: 1941 525
BmrI ACTGGGnnnn_n'
Cuts at: 0 309 1446 1975 2357 2466
Size: 309 1137 529 382 109
Fragments arranged by size:
1137 529 382 309 109
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1434 1575 2226 2319 2466
Size: 1434 141 651 93 147
Fragments arranged by size:
1434 651 147 141 93
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 232 373 415 730 778 1114 1165 1270
Size: 232 141 42 315 48 336 51 105
Cuts at: 1270 1411 1846 1876 2053 2314 2466
Size: 141 435 30 177 261 152
Fragments arranged by size:
435 336 315 261 232 177 152 141
141 105 51 48 42 30
Bpu10I CC'TnA_GC
Cuts at: 0 437 2466
Size: 437 2029
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 561 1066 1117 1870 2466
Size: 561 505 51 753 596
Fragments arranged by size:
753 596 561 505 51
BsaAI yAC'GTr
Cuts at: 0 981 1178 2466
Size: 981 197 1288
Fragments arranged by size:
1288 981 197
BsaBI GATnn'nnATC
Cuts at: 0 1460 2466
Size: 1460 1006
BsaHI Gr'CG_yC
Cuts at: 0 1169 2438 2466
Size: 1169 1269 28
Fragments arranged by size:
1269 1169 28
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 22 52 205 235 832 862 2466
Size: 22 30 153 30 597 30 1604
Fragments arranged by size:
1604 597 153 30 30 30 22
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 172 229 1039 1741 1765 2134 2164 2167
Size: 172 57 810 702 24 369 30 3
Cuts at: 2167 2257 2260 2466
Size: 90 3 206
Fragments arranged by size:
810 702 369 206 172 90 57 30
24 3 3
BseYI C'CCAG_C
Cuts at: 0 108 1318 2466
Size: 108 1210 1148
Fragments arranged by size:
1210 1148 108
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 158 683 690 778 1567 2339 2466
Size: 158 525 7 88 789 772 127
Fragments arranged by size:
789 772 525 158 127 88 7
BsiEI CG_ry'CG
Cuts at: 0 1575 1956 2435 2466
Size: 1575 381 479 31
Fragments arranged by size:
1575 479 381 31
BsiHKAI G_wGCw'C
Cuts at: 0 85 164 438 1031 1480 2186 2466
Size: 85 79 274 593 449 706 280
Fragments arranged by size:
706 593 449 280 274 85 79
BsmI GAATG_Cn'
Cuts at: 0 282 2466
Size: 282 2184
Bsp1286I G_dGCh'C
Cuts at: 0 85 164 314 430 438 583 655 1031
Size: 85 79 150 116 8 145 72 376
Cuts at: 1031 1480 2126 2186 2466
Size: 449 646 60 280
Fragments arranged by size:
646 449 376 280 150 145 116 85
79 72 60 8
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 383 2381 2402 2466
Size: 383 1998 21 64
Fragments arranged by size:
1998 383 64 21
BsrBI CCG'CTC
Cuts at: 0 762 882 1559 1608 2157 2466
Size: 762 120 677 49 549 309
Fragments arranged by size:
762 677 549 309 120 49
BsrFI r'CCGG_y
Cuts at: 0 85 99 614 629 638 949 2434 2466
Size: 85 14 515 15 9 311 1485 32
Fragments arranged by size:
1485 515 311 85 32 15 14 9
BssSI C'ACGA_G
Cuts at: 0 1123 2466
Size: 1123 1343
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1119 2109 2382 2466
Size: 1119 990 273 84
Fragments arranged by size:
1119 990 273 84
BstEII G'GTnAC_C
Cuts at: 0 801 1929 1974 2466
Size: 801 1128 45 492
Fragments arranged by size:
1128 801 492 45
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1436 1941 2466
Size: 1436 505 525
Fragments arranged by size:
1436 525 505
BstYI r'GATC_y
Cuts at: 0 366 1613 2466
Size: 366 1247 853
Fragments arranged by size:
1247 853 366
Bsu36I CC'TnA_GG
Cuts at: 0 1282 2162 2466
Size: 1282 880 304
Fragments arranged by size:
1282 880 304
BtgI C'CryG_G
Cuts at: 0 192 1238 1934 2063 2466
Size: 192 1046 696 129 403
Fragments arranged by size:
1046 696 403 192 129
BtsI GCAGTG_nn'
Cuts at: 0 681 2466
Size: 681 1785
DraIII CAC_nnn'GTG
Cuts at: 0 801 2466
Size: 801 1665
EaeI y'GGCC_r
Cuts at: 0 1063 1568 1741 1801 1949 2360 2432 2466
Size: 1063 505 173 60 148 411 72 34
Fragments arranged by size:
1063 505 411 173 148 72 60 34
EagI C'GGCC_G
Cuts at: 0 2432 2466
Size: 2432 34
EarI CTCTTCn'nnn_
Cuts at: 0 149 1036 1546 2263 2466
Size: 149 887 510 717 203
Fragments arranged by size:
887 717 510 203 149
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 37 1261 1708 2097 2466
Size: 37 1224 447 389 369
Fragments arranged by size:
1224 447 389 369 37
EcoICRI GAG'CTC
Cuts at: 0 162 1029 1478 2466
Size: 162 867 449 988
Fragments arranged by size:
988 867 449 162
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 232 373 415 730 778 1017 1114 1165
Size: 232 141 42 315 48 239 97 51
Cuts at: 1165 1270 1411 1581 1654 1702 1846 1876 2053
Size: 105 141 170 73 48 144 30 177
Cuts at: 2053 2314 2466
Size: 261 152
Fragments arranged by size:
315 261 239 232 177 170 152 144
141 141 105 97 73 51 48 48
42 30
EcoNI CCTnn'n_nnAGG
Cuts at: 0 796 2466
Size: 796 1670
EcoO109I rG'GnC_Cy
Cuts at: 0 1077 1350 1440 1536 1653 1830 2466
Size: 1077 273 90 96 117 177 636
Fragments arranged by size:
1077 636 273 177 117 96 90
EcoRV GAT'ATC
Cuts at: 0 1842 2466
Size: 1842 624
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1557 1659 2466
Size: 1557 102 807
Fragments arranged by size:
1557 807 102
FspI TGC'GCA
Cuts at: 0 1018 2466
Size: 1018 1448
HaeII r_GCGC'y
Cuts at: 0 849 975 1219 2071 2466
Size: 849 126 244 852 395
Fragments arranged by size:
852 849 395 244 126
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 22 54 440 472 507 539 2466
Size: 22 32 386 32 35 32 1927
Fragments arranged by size:
1927 386 35 32 32 32 22
HincII GTy'rAC
Cuts at: 0 1173 2466
Size: 1173 1293
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1888 2466
Size: 1888 578
MslI CAynn'nnrTG
Cuts at: 0 1667 1939 2338 2466
Size: 1667 272 399 128
Fragments arranged by size:
1667 399 272 128
MspA1I CmG'CkG
Cuts at: 0 134 657 968 1119 2024 2466
Size: 134 523 311 151 905 442
Fragments arranged by size:
905 523 442 311 151 134
NaeI GCC'GGC
Cuts at: 0 101 951 2436 2466
Size: 101 850 1485 30
Fragments arranged by size:
1485 850 101 30
NcoI C'CATG_G
Cuts at: 0 1238 1934 2466
Size: 1238 696 532
Fragments arranged by size:
1238 696 532
NgoMIV G'CCGG_C
Cuts at: 0 99 949 2434 2466
Size: 99 850 1485 32
Fragments arranged by size:
1485 850 99 32
NspI r_CATG'y
Cuts at: 0 2328 2337 2466
Size: 2328 9 129
Fragments arranged by size:
2328 129 9
PasI CC'CwG_GG
Cuts at: 0 1920 2466
Size: 1920 546
PflMI CCAn_nnn'nTGG
Cuts at: 0 708 2466
Size: 708 1758
PfoI T'CCnGG_A
Cuts at: 0 124 209 1247 1442 1721 2466
Size: 124 85 1038 195 279 745
Fragments arranged by size:
1038 745 279 195 124 85
PmlI CAC'GTG
Cuts at: 0 981 1178 2466
Size: 981 197 1288
Fragments arranged by size:
1288 981 197
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 205 237 2466
Size: 205 32 2229
Fragments arranged by size:
2229 205 32
PpuMI rG'GwC_Cy
Cuts at: 0 1077 1350 1440 1536 1830 2466
Size: 1077 273 90 96 294 636
Fragments arranged by size:
1077 636 294 273 96 90
PstI C_TGCA'G
Cuts at: 0 239 251 650 917 2102 2378 2387 2399
Size: 239 12 399 267 1185 276 9 12
Cuts at: 2399 2464 2466
Size: 65 2
Fragments arranged by size:
1185 399 276 267 239 65 12 12
9 2
PvuII CAG'CTG
Cuts at: 0 1119 2024 2466
Size: 1119 905 442
Fragments arranged by size:
1119 905 442
SacI G_AGCT'C
Cuts at: 0 164 1031 1480 2466
Size: 164 867 449 986
Fragments arranged by size:
986 867 449 164
SanDI GG'GwC_CC
Cuts at: 0 1440 2466
Size: 1440 1026
SapI GCTCTTCn'nnn_
Cuts at: 0 1036 2263 2466
Size: 1036 1227 203
Fragments arranged by size:
1227 1036 203
SbfI CC_TGCA'GG
Cuts at: 0 917 2378 2466
Size: 917 1461 88
Fragments arranged by size:
1461 917 88
ScaI AGT'ACT
Cuts at: 0 1087 2466
Size: 1087 1379
SexAI A'CCwGG_T
Cuts at: 0 797 2466
Size: 797 1669
SfcI C'TryA_G
Cuts at: 0 235 247 646 913 2098 2374 2383 2395
Size: 235 12 399 267 1185 276 9 12
Cuts at: 2395 2460 2466
Size: 65 6
Fragments arranged by size:
1185 399 276 267 235 65 12 12
9 6
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 2227 2466
Size: 2227 239
SmaI CCC'GGG
Cuts at: 0 808 2466
Size: 808 1658
SmlI C'TyrA_G
Cuts at: 0 540 1081 1132 1885 2466
Size: 540 541 51 753 581
Fragments arranged by size:
753 581 541 540 51
StyI C'CwwG_G
Cuts at: 0 1238 1934 2466
Size: 1238 696 532
Fragments arranged by size:
1238 696 532
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 195 521 1390 2466
Size: 195 326 869 1076
Fragments arranged by size:
1076 869 326 195
TatI w'GTAC_w
Cuts at: 0 1085 1983 2466
Size: 1085 898 483
Fragments arranged by size:
1085 898 483
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 120 152 2466
Size: 120 32 2314
Fragments arranged by size:
2314 120 32
Tth111I GACn'n_nGTC
Cuts at: 0 1438 2466
Size: 1438 1028
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 526 1245 2028 2466
Size: 526 719 783 438
Fragments arranged by size:
783 719 526 438
XmaI C'CCGG_G
Cuts at: 0 806 2466
Size: 806 1660
Enzymes that do cut:
AccI AcuI AfeI AflIII AleI AlfI AloI AlwNI
AvaI BamHI BanI BanII BbsI BcgI BglI BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaAI
BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspMI BsrBI BsrFI BssSI BstAPI BstEII
BstXI BstYI Bsu36I BtgI BtsI DraIII EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRV FalI
FspI HaeII Hin4I HincII MmeI MslI MspA1I NaeI
NcoI NgoMIV NspI PasI PflMI PfoI PmlI PpiI
PpuMI PstI PvuII SacI SanDI SapI SbfI ScaI
SexAI SfcI SfiI SmaI SmlI StyI TaqII TatI
TstI Tth111I XcmI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AgeI AhdI ApaI
ApaLI ApoI AscI AseI AsiSI AvrII BaeI BbeI
BbvCI BciVI BclI BfrBI BglII BmtI BsaI BsaWI
BsiWI BsmBI BspEI BspHI BsrDI BsrGI BssHII BstBI
BstZ17I BtgZI ClaI CspCI DraI DrdI EcoRI FseI
FspAI HindIII HpaI KasI KpnI MfeI MluI MscI
NarI NdeI NheI NotI NruI NsiI PacI PciI
PmeI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII
SacII SalI SfoI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI StuI SwaI XbaI XhoI XmnI ZraI