(Linear) (Six-base) MAPSORT of: KIEE3631.seq  Check: 3978  from: 1  to: 942



KIEE3631.orf

 With 182 enzymes: SgfI * 

                        June 27, 2011 16:05  ..

AflIII A'CryG_T

Cuts at:      0     692     942 
   Size:        692     250
 
ApoI r'AATT_y

Cuts at:      0     883     942 
   Size:        883      59
 
AseI AT'TA_AT

Cuts at:      0      14     942 
   Size:         14     928
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     707     942 
   Size:        707     235
 
BbvCI CC'TCA_GC

Cuts at:      0     800     942 
   Size:        800     142
 
BlpI GC'TnA_GC

Cuts at:      0     417     942 
   Size:        417     525
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     146     942 
   Size:        146     796
 
Bpu10I CC'TnA_GC

Cuts at:      0     800     942 
   Size:        800     142
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      52     942 
   Size:         52     890
 
BsaBI GATnn'nnATC

Cuts at:      0     329     942 
   Size:        329     613
 
BsiHKAI G_wGCw'C

Cuts at:      0     692     942 
   Size:        692     250
 
Bsp1286I G_dGCh'C

Cuts at:      0     692     942 
   Size:        692     250
 
BsrDI GCAATG_nn'

Cuts at:      0     166     942 
   Size:        166     776
 
BsrGI T'GTAC_A

Cuts at:      0     888     942 
   Size:        888      54
 
BstEII G'GTnAC_C

Cuts at:      0     222     942 
   Size:        222     720
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     644     942 
   Size:        644     298
 
DraIII CAC_nnn'GTG

Cuts at:      0     427     942 
   Size:        427     515
 
EarI CTCTTCn'nnn_

Cuts at:      0     104     425     476     942 
   Size:        104     321      51     466

  Fragments arranged by size: 

                 466     321     104      51
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     329     942 
   Size:        329     613
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     146     942 
   Size:        146     796
 
HindIII A'AGCT_T

Cuts at:      0     539     942 
   Size:        539     403
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     175     476     928     942 
   Size:        175     301     452      14

  Fragments arranged by size: 

                 452     301     175      14
 
MslI CAynn'nnrTG

Cuts at:      0     159     642     695     942 
   Size:        159     483      53     247

  Fragments arranged by size: 

                 483     247     159      53
 
MspA1I CmG'CkG

Cuts at:      0     124     942 
   Size:        124     818
 
NdeI CA'TA_TG

Cuts at:      0     860     942 
   Size:        860      82
 
NspI r_CATG'y

Cuts at:      0     696     799     942 
   Size:        696     103     143

  Fragments arranged by size: 

                 696     143     103
 
PciI A'CATG_T

Cuts at:      0     692     942 
   Size:        692     250
 
PshAI GACnn'nnGTC

Cuts at:      0      61     707     942 
   Size:         61     646     235

  Fragments arranged by size: 

                 646     235      61
 
PstI C_TGCA'G

Cuts at:      0     465     942 
   Size:        465     477
 
PvuII CAG'CTG

Cuts at:      0     124     942 
   Size:        124     818
 
SfcI C'TryA_G

Cuts at:      0     167     461     942 
   Size:        167     294     481

  Fragments arranged by size: 

                 481     294     167
 
SmlI C'TyrA_G

Cuts at:      0      67     942 
   Size:         67     875
 
SphI G_CATG'C

Cuts at:      0     799     942 
   Size:        799     143
 
SspI AAT'ATT

Cuts at:      0     268     625     942 
   Size:        268     357     317

  Fragments arranged by size: 

                 357     317     268
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     800     942 
   Size:        800     142
 
TatI w'GTAC_w

Cuts at:      0     888     942 
   Size:        888      54
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     158     942 
   Size:        158     784
 

 Enzymes that do cut:

   AflIII     ApoI     AseI     BbsI    BbvCI     BlpI     BpmI   Bpu10I
    BpuEI    BsaBI  BsiHKAI Bsp1286I    BsrDI    BsrGI   BstEII    BstXI
   DraIII     EarI     EciI  Eco57MI  HindIII     MmeI     MslI   MspA1I
     NdeI     NspI     PciI    PshAI     PstI    PvuII     SfcI     SmlI
     SphI     SspI    TaqII     TatI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI     BcgI    BciVI     BclI    BfrBI     BglI    BglII Bme1580I
    BmgBI     BmrI     BmtI     BplI     BsaI    BsaAI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI    BsiWI     BsmI    BsmBI
    BspEI    BspHI    BspMI    BsrBI    BsrFI   BssHII    BssSI   BstAPI
    BstBI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI
    CspCI     DraI     DrdI     EaeI     EagI  EcoICRI    EcoNI EcoO109I
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I
   HincII     HpaI     KasI     KpnI     MfeI     MluI     MscI     NaeI
     NarI     NcoI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
     PasI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI     PsiI
   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SrfI     StuI     StyI     SwaI
     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI