Restriction map for
pFN21AE3630
(Linear) (Six-base) MAPSORT of: KIEE3630.seq Check: 3447 from: 1 to: 1623
KIEE3630.orf
With 182 enzymes: SgfI *
December 27, 2011 16:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 684 1623
Size: 684 939
AccI GT'mk_AC
Cuts at: 0 493 1623
Size: 493 1130
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 520 875 1421 1623
Size: 520 355 546 202
Fragments arranged by size:
546 520 355 202
AlwNI CAG_nnn'CTG
Cuts at: 0 658 885 1218 1537 1623
Size: 658 227 333 319 86
Fragments arranged by size:
658 333 319 227 86
ApaI G_GGCC'C
Cuts at: 0 685 821 1623
Size: 685 136 802
Fragments arranged by size:
802 685 136
ApoI r'AATT_y
Cuts at: 0 1006 1222 1623
Size: 1006 216 401
Fragments arranged by size:
1006 401 216
AvaI C'yCGr_G
Cuts at: 0 638 1066 1291 1623
Size: 638 428 225 332
Fragments arranged by size:
638 428 332 225
BanI G'GyrC_C
Cuts at: 0 643 1062 1308 1582 1623
Size: 643 419 246 274 41
Fragments arranged by size:
643 419 274 246 41
BanII G_rGCy'C
Cuts at: 0 523 685 821 1623
Size: 523 162 136 802
Fragments arranged by size:
802 523 162 136
BbsI GAAGACnn'nnnn_
Cuts at: 0 863 1468 1574 1623
Size: 863 605 106 49
Fragments arranged by size:
863 605 106 49
BbvCI CC'TCA_GC
Cuts at: 0 1247 1623
Size: 1247 376
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1052 1086 1371 1405 1623
Size: 1052 34 285 34 218
Fragments arranged by size:
1052 285 218 34 34
BclI T'GATC_A
Cuts at: 0 887 1265 1623
Size: 887 378 358
Fragments arranged by size:
887 378 358
BglI GCCn_nnn'nGGC
Cuts at: 0 426 1060 1623
Size: 426 634 563
Fragments arranged by size:
634 563 426
BglII A'GATC_T
Cuts at: 0 1091 1623
Size: 1091 532
Bme1580I G_kGCm'C
Cuts at: 0 685 821 836 974 1048 1165 1585 1623
Size: 685 136 15 138 74 117 420 38
Fragments arranged by size:
685 420 138 136 117 74 38 15
BmgBI CAC'GTC
Cuts at: 0 450 625 1623
Size: 450 175 998
Fragments arranged by size:
998 450 175
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1092 1539 1623
Size: 1092 447 84
Fragments arranged by size:
1092 447 84
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 241 680 1561 1623
Size: 241 439 881 62
Fragments arranged by size:
881 439 241 62
Bpu10I CC'TnA_GC
Cuts at: 0 1247 1623
Size: 1247 376
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 147 703 1623
Size: 147 556 920
Fragments arranged by size:
920 556 147
BsaI GGTCTCn'nnnn_
Cuts at: 0 1075 1623
Size: 1075 548
BsaAI yAC'GTr
Cuts at: 0 1474 1623
Size: 1474 149
BsaWI w'CCGG_w
Cuts at: 0 283 1623
Size: 283 1340
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 436 466 1623
Size: 436 30 1157
Fragments arranged by size:
1157 436 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 243 1018 1420 1621 1623
Size: 243 775 402 201 2
Fragments arranged by size:
775 402 243 201 2
BseYI C'CCAG_C
Cuts at: 0 25 57 1435 1623
Size: 25 32 1378 188
Fragments arranged by size:
1378 188 32 25
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 499 1309 1365 1623
Size: 499 810 56 258
Fragments arranged by size:
810 499 258 56
BsiEI CG_ry'CG
Cuts at: 0 288 430 1623
Size: 288 142 1193
Fragments arranged by size:
1193 288 142
BsiHKAI G_wGCw'C
Cuts at: 0 1619 1623
Size: 1619 4
BsmBI CGTCTCn'nnnn_
Cuts at: 0 456 1623
Size: 456 1167
Bsp1286I G_dGCh'C
Cuts at: 0 523 685 821 836 974 1048 1165 1585
Size: 523 162 136 15 138 74 117 420
Cuts at: 1585 1619 1623
Size: 34 4
Fragments arranged by size:
523 420 162 138 136 117 74 34
15 4
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 684 1623
Size: 684 939
BsrBI CCG'CTC
Cuts at: 0 309 1179 1623
Size: 309 870 444
Fragments arranged by size:
870 444 309
BsrDI GCAATG_nn'
Cuts at: 0 581 1008 1623
Size: 581 427 615
Fragments arranged by size:
615 581 427
BsrFI r'CCGG_y
Cuts at: 0 212 331 613 1508 1623
Size: 212 119 282 895 115
Fragments arranged by size:
895 282 212 119 115
BsrGI T'GTAC_A
Cuts at: 0 788 1623
Size: 788 835
BssSI C'ACGA_G
Cuts at: 0 1338 1623
Size: 1338 285
BstBI TT'CG_AA
Cuts at: 0 800 1623
Size: 800 823
BstEII G'GTnAC_C
Cuts at: 0 162 1623
Size: 162 1461
BstXI CCAn_nnnn'nTGG
Cuts at: 0 954 1041 1623
Size: 954 87 582
Fragments arranged by size:
954 582 87
BstYI r'GATC_y
Cuts at: 0 882 1091 1623
Size: 882 209 532
Fragments arranged by size:
882 532 209
BstZ17I GTA'TAC
Cuts at: 0 494 1623
Size: 494 1129
BtgI C'CryG_G
Cuts at: 0 1040 1124 1598 1623
Size: 1040 84 474 25
Fragments arranged by size:
1040 474 84 25
BtsI GCAGTG_nn'
Cuts at: 0 1023 1623
Size: 1023 600
DraIII CAC_nnn'GTG
Cuts at: 0 170 1623
Size: 170 1453
DrdI GACnn_nn'nnGTC
Cuts at: 0 438 1623
Size: 438 1185
EaeI y'GGCC_r
Cuts at: 0 231 427 649 1155 1623
Size: 231 196 222 506 468
Fragments arranged by size:
506 468 231 222 196
EagI C'GGCC_G
Cuts at: 0 427 1623
Size: 427 1196
EarI CTCTTCn'nnn_
Cuts at: 0 1397 1623
Size: 1397 226
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1366 1623
Size: 1366 257
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 241 520 680 875 1421 1561 1623
Size: 241 279 160 195 546 140 62
Fragments arranged by size:
546 279 241 195 160 140 62
EcoO109I rG'GnC_Cy
Cuts at: 0 105 114 240 260 681 682 759 817
Size: 105 9 126 20 421 1 77 58
Cuts at: 817 1215 1260 1623
Size: 398 45 363
Fragments arranged by size:
421 398 363 126 105 77 58 45
20 9 1
EcoRI G'AATT_C
Cuts at: 0 1006 1623
Size: 1006 617
HaeII r_GCGC'y
Cuts at: 0 1537 1623
Size: 1537 86
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 347 379 653 685 1194 1226 1623
Size: 347 32 274 32 509 32 397
Fragments arranged by size:
509 397 347 274 32 32 32
HincII GTy'rAC
Cuts at: 0 810 1623
Size: 810 813
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 939 971 1058 1346 1623
Size: 939 32 87 288 277
Fragments arranged by size:
939 288 277 87 32
MscI TGG'CCA
Cuts at: 0 233 1157 1623
Size: 233 924 466
Fragments arranged by size:
924 466 233
MslI CAynn'nnrTG
Cuts at: 0 463 737 1129 1273 1623
Size: 463 274 392 144 350
Fragments arranged by size:
463 392 350 274 144
MspA1I CmG'CkG
Cuts at: 0 483 1623
Size: 483 1140
NaeI GCC'GGC
Cuts at: 0 615 1510 1623
Size: 615 895 113
Fragments arranged by size:
895 615 113
NcoI C'CATG_G
Cuts at: 0 1124 1623
Size: 1124 499
NgoMIV G'CCGG_C
Cuts at: 0 613 1508 1623
Size: 613 895 115
Fragments arranged by size:
895 613 115
PasI CC'CwG_GG
Cuts at: 0 315 677 1331 1623
Size: 315 362 654 292
Fragments arranged by size:
654 362 315 292
PflMI CCAn_nnn'nTGG
Cuts at: 0 658 1623
Size: 658 965
PfoI T'CCnGG_A
Cuts at: 0 657 761 878 1623
Size: 657 104 117 745
Fragments arranged by size:
745 657 117 104
PpuMI rG'GwC_Cy
Cuts at: 0 240 260 759 1260 1623
Size: 240 20 499 501 363
Fragments arranged by size:
501 499 363 240 20
PspOMI G'GGCC_C
Cuts at: 0 681 817 1623
Size: 681 136 806
Fragments arranged by size:
806 681 136
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 781 813 1623
Size: 781 32 810
Fragments arranged by size:
810 781 32
PvuII CAG'CTG
Cuts at: 0 483 1623
Size: 483 1140
SanDI GG'GwC_CC
Cuts at: 0 260 1623
Size: 260 1363
ScaI AGT'ACT
Cuts at: 0 904 1623
Size: 904 719
SmlI C'TyrA_G
Cuts at: 0 126 718 1623
Size: 126 592 905
Fragments arranged by size:
905 592 126
SnaBI TAC'GTA
Cuts at: 0 1474 1623
Size: 1474 149
StuI AGG'CCT
Cuts at: 0 1108 1623
Size: 1108 515
StyI C'CwwG_G
Cuts at: 0 813 1109 1124 1623
Size: 813 296 15 499
Fragments arranged by size:
813 499 296 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1117 1623
Size: 1117 506
TatI w'GTAC_w
Cuts at: 0 788 902 1623
Size: 788 114 721
Fragments arranged by size:
788 721 114
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 436 468 1623
Size: 436 32 1155
Fragments arranged by size:
1155 436 32
XmnI GAAnn'nnTTC
Cuts at: 0 1495 1623
Size: 1495 128
Enzymes that do cut:
AarI AccI AcuI AlwNI ApaI ApoI AvaI BanI
BanII BbsI BbvCI BcgI BclI BglI BglII Bme1580I
BmgBI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstBI BstEII
BstXI BstYI BstZ17I BtgI BtsI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI HaeII Hin4I
HincII MmeI MscI MslI MspA1I NaeI NcoI NgoMIV
PasI PflMI PfoI PpuMI PspOMI PsrI PvuII SanDI
ScaI SmlI SnaBI StuI StyI TaqII TatI TstI
XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BciVI BfrBI BlpI BmrI BmtI
BsaBI BsaHI BsiWI BsmI BspEI BspHI BssHII BstAPI
Bsu36I BtgZI ClaI CspCI DraI EcoICRI EcoNI EcoRV
FalI FseI FspI FspAI HindIII HpaI KasI KpnI
MfeI MluI NarI NdeI NheI NotI NruI NsiI
NspI PacI PciI PmeI PmlI PpiI PshAI PsiI
PspXI PstI PvuI RsrII SacI SacII SalI SapI
SbfI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI
SpeI SphI SrfI SspI SwaI Tth111I XbaI XcmI
XhoI XmaI ZraI