(Linear) (Six-base) MAPSORT of: KIEE3627.seq  Check: 8282  from: 1  to: 342



KIEE3627.orf

 With 182 enzymes: SgfI * 

                        December 27, 2011 16:36  ..

AatII G_ACGT'C

Cuts at:      0     167     342 
   Size:        167     175
 
AlwNI CAG_nnn'CTG

Cuts at:      0     272     342 
   Size:        272      70
 
AvaI C'yCGr_G

Cuts at:      0      19     322     342 
   Size:         19     303      20

  Fragments arranged by size: 

                 303      20      19
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     167     342 
   Size:        167     175
 
Bme1580I G_kGCm'C

Cuts at:      0      13     342 
   Size:         13     329
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     249     342 
   Size:        249      93
 
BsaBI GATnn'nnATC

Cuts at:      0     252     342 
   Size:        252      90
 
BsaHI Gr'CG_yC

Cuts at:      0     164     342 
   Size:        164     178
 
BsiHKAI G_wGCw'C

Cuts at:      0     149     342 
   Size:        149     193
 
Bsp1286I G_dGCh'C

Cuts at:      0      13     149     342 
   Size:         13     136     193

  Fragments arranged by size: 

                 193     136      13
 
EaeI y'GGCC_r

Cuts at:      0       2     342 
   Size:          2     340
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     249     342 
   Size:        249      93
 
HaeII r_GCGC'y

Cuts at:      0     279     342 
   Size:        279      63
 
NspI r_CATG'y

Cuts at:      0     318     342 
   Size:        318      24
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     272     342 
   Size:        272      70
 
RsrII CG'GwC_CG

Cuts at:      0      45     342 
   Size:         45     297
 
SmlI C'TyrA_G

Cuts at:      0     322     342 
   Size:        322      20
 
StuI AGG'CCT

Cuts at:      0     180     342 
   Size:        180     162
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     208     342 
   Size:        208     134
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      37      69     342 
   Size:         37      32     273

  Fragments arranged by size: 

                 273      37      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     166     342 
   Size:        166     176
 
XhoI C'TCGA_G

Cuts at:      0     322     342 
   Size:        322      20
 
ZraI GAC'GTC

Cuts at:      0     165     342 
   Size:        165     177
 

 Enzymes that do cut:

    AatII    AlwNI     AvaI     BbsI Bme1580I     BpmI    BsaBI    BsaHI
  BsiHKAI Bsp1286I     EaeI  Eco57MI    HaeII     NspI    PflMI    RsrII
     SmlI     StuI    TaqII     TstI  Tth111I     XhoI     ZraI

 Enzymes that do not cut: 

     AarI     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI    BsiWI
     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI
  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EagI     EarI     EciI  EcoICRI    EcoNI EcoO109I
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    Hin4I   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PasI     PciI     PfoI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    PvuII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StyI     SwaI
     TatI     XbaI     XcmI     XmaI     XmnI