Restriction map for
pFN21AE3626
(Linear) (Six-base) MAPSORT of: KIEE3626.seq Check: 6262 from: 1 to: 648
KIEE3626.orf
With 182 enzymes: SgfI *
December 27, 2011 16:36 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 428 648
Size: 428 220
AhdI GACnn_n'nnGTC
Cuts at: 0 369 627 648
Size: 369 258 21
Fragments arranged by size:
369 258 21
AleI CACnn'nnGTG
Cuts at: 0 46 648
Size: 46 602
ApaI G_GGCC'C
Cuts at: 0 566 648
Size: 566 82
BanII G_rGCy'C
Cuts at: 0 135 566 648
Size: 135 431 82
Fragments arranged by size:
431 135 82
BciVI GTATCCnnnnn_n'
Cuts at: 0 174 648
Size: 174 474
Bme1580I G_kGCm'C
Cuts at: 0 566 648
Size: 566 82
BmrI ACTGGGnnnn_n'
Cuts at: 0 590 648
Size: 590 58
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 260 648
Size: 260 388
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 55 648
Size: 55 593
BseYI C'CCAG_C
Cuts at: 0 187 605 648
Size: 187 418 43
Fragments arranged by size:
418 187 43
BsiHKAI G_wGCw'C
Cuts at: 0 135 246 648
Size: 135 111 402
Fragments arranged by size:
402 135 111
Bsp1286I G_dGCh'C
Cuts at: 0 135 246 566 648
Size: 135 111 320 82
Fragments arranged by size:
320 135 111 82
BsrDI GCAATG_nn'
Cuts at: 0 468 648
Size: 468 180
BssSI C'ACGA_G
Cuts at: 0 617 648
Size: 617 31
BstYI r'GATC_y
Cuts at: 0 509 648
Size: 509 139
BtsI GCAGTG_nn'
Cuts at: 0 244 648
Size: 244 404
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 101 136 569 604 648
Size: 101 35 433 35 44
Fragments arranged by size:
433 101 44 35 35
EarI CTCTTCn'nnn_
Cuts at: 0 22 382 464 648
Size: 22 360 82 184
Fragments arranged by size:
360 184 82 22
EcoICRI GAG'CTC
Cuts at: 0 133 648
Size: 133 515
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 260 428 648
Size: 260 168 220
Fragments arranged by size:
260 220 168
EcoNI CCTnn'n_nnAGG
Cuts at: 0 225 411 648
Size: 225 186 237
Fragments arranged by size:
237 225 186
EcoO109I rG'GnC_Cy
Cuts at: 0 563 592 648
Size: 563 29 56
Fragments arranged by size:
563 56 29
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 355 387 613 645 648
Size: 355 32 226 32 3
Fragments arranged by size:
355 226 32 32 3
HincII GTy'rAC
Cuts at: 0 144 648
Size: 144 504
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 353 648
Size: 353 295
MslI CAynn'nnrTG
Cuts at: 0 46 648
Size: 46 602
MspA1I CmG'CkG
Cuts at: 0 238 609 648
Size: 238 371 39
Fragments arranged by size:
371 238 39
PpuMI rG'GwC_Cy
Cuts at: 0 592 648
Size: 592 56
PspOMI G'GGCC_C
Cuts at: 0 562 648
Size: 562 86
PstI C_TGCA'G
Cuts at: 0 74 648
Size: 74 574
PvuII CAG'CTG
Cuts at: 0 238 648
Size: 238 410
SacI G_AGCT'C
Cuts at: 0 135 648
Size: 135 513
SanDI GG'GwC_CC
Cuts at: 0 592 648
Size: 592 56
SfcI C'TryA_G
Cuts at: 0 70 225 648
Size: 70 155 423
Fragments arranged by size:
423 155 70
StuI AGG'CCT
Cuts at: 0 406 475 648
Size: 406 69 173
Fragments arranged by size:
406 173 69
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 310 342 345 377 603 635 648
Size: 310 32 3 32 226 32 13
Fragments arranged by size:
310 226 32 32 32 13 3
Enzymes that do cut:
AcuI AhdI AleI ApaI BanII BciVI Bme1580I BmrI
BpmI BseRI BseYI BsiHKAI Bsp1286I BsrDI BssSI BstYI
BtsI CspCI EarI EcoICRI Eco57MI EcoNI EcoO109I Hin4I
HincII MmeI MslI MspA1I PpuMI PspOMI PstI PvuII
SacI SanDI SfcI StuI TstI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AlfI AloI AlwNI ApaLI ApoI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BbeI BbsI
BbvCI BcgI BclI BfrBI BglI BglII BlpI BmgBI
BmtI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BsgI BsiEI BsiWI BsmI BsmBI BspEI
BspHI BspMI BsrBI BsrFI BsrGI BssHII BstAPI BstBI
BstEII BstXI BstZ17I Bsu36I BtgI BtgZI ClaI DraI
DraIII DrdI EaeI EagI EciI EcoRI EcoRV FalI
FseI FspI FspAI HaeII HindIII HpaI KasI KpnI
MfeI MluI MscI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI PacI PasI PciI
PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspXI
PsrI PvuI RsrII SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StyI SwaI TaqII TatI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI