(Linear) (Six-base) MAPSORT of: KIEE3623.seq  Check: 9433  from: 1  to: 444



KIEE3623.orf

 With 182 enzymes: SgfI * 

                        December 27, 2011 16:36  ..

AatII G_ACGT'C

Cuts at:      0     158     444 
   Size:        158     286
 
AfeI AGC'GCT

Cuts at:      0      14     444 
   Size:         14     430
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      96     444 
   Size:         96     348
 
BanI G'GyrC_C

Cuts at:      0      20     444 
   Size:         20     424
 
BbeI G_GCGC'C

Cuts at:      0      24     444 
   Size:         24     420
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      29     444 
   Size:         29     415
 
BsaHI Gr'CG_yC

Cuts at:      0      21     155     444 
   Size:         21     134     289

  Fragments arranged by size: 

                 289     134      21
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     198     294     444 
   Size:        198      96     150

  Fragments arranged by size: 

                 198     150      96
 
BsrFI r'CCGG_y

Cuts at:      0     280     444 
   Size:        280     164
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     237     261     444 
   Size:        237      24     183

  Fragments arranged by size: 

                 237     183      24
 
BtgI C'CryG_G

Cuts at:      0     314     395     444 
   Size:        314      81      49

  Fragments arranged by size: 

                 314      81      49
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      29     444 
   Size:         29     415
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     265     444 
   Size:        265     179
 
EcoO109I rG'GnC_Cy

Cuts at:      0     336     444 
   Size:        336     108
 
HaeII r_GCGC'y

Cuts at:      0      16      24     444 
   Size:         16       8     420

  Fragments arranged by size: 

                 420      16       8
 
KasI G'GCGC_C

Cuts at:      0      20     444 
   Size:         20     424
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      80     329     444 
   Size:         80     249     115

  Fragments arranged by size: 

                 249     115      80
 
MslI CAynn'nnrTG

Cuts at:      0     220     444 
   Size:        220     224
 
MspA1I CmG'CkG

Cuts at:      0     363     444 
   Size:        363      81
 
NarI GG'CG_CC

Cuts at:      0      21     444 
   Size:         21     423
 
NcoI C'CATG_G

Cuts at:      0     395     444 
   Size:        395      49
 
NspI r_CATG'y

Cuts at:      0      99     444 
   Size:         99     345
 
PasI CC'CwG_GG

Cuts at:      0     267     444 
   Size:        267     177
 
PpuMI rG'GwC_Cy

Cuts at:      0     336     444 
   Size:        336     108
 
SfoI GGC'GCC

Cuts at:      0      22     444 
   Size:         22     422
 
StyI C'CwwG_G

Cuts at:      0     395     444 
   Size:        395      49
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      50     444 
   Size:         50     394
 
TatI w'GTAC_w

Cuts at:      0     191     444 
   Size:        191     253
 
Tth111I GACn'n_nGTC

Cuts at:      0     322     444 
   Size:        322     122
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     312     402     444 
   Size:        312      90      42

  Fragments arranged by size: 

                 312      90      42
 
XmnI GAAnn'nnTTC

Cuts at:      0     127     444 
   Size:        127     317
 
ZraI GAC'GTC

Cuts at:      0     156     444 
   Size:        156     288
 

 Enzymes that do cut:

    AatII     AfeI     AlfI     BanI     BbeI     BpmI    BsaHI     BsgI
    BsrFI    BstXI     BtgI  Eco57MI    EcoNI EcoO109I    HaeII     KasI
     MmeI     MslI   MspA1I     NarI     NcoI     NspI     PasI    PpuMI
     SfoI     StyI    TaqII     TatI  Tth111I     XcmI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AloI    AlwNI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII     BbsI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaWI    BsaXI    BseRI    BseYI    BsiEI  BsiHKAI
    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstYI
  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII
     DrdI     EaeI     EagI     EarI     EciI  EcoICRI    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    Hin4I   HincII  HindIII     HpaI
     KpnI     MfeI     MluI     MscI     NaeI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfcI     SfiI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
     TstI     XbaI     XhoI     XmaI