Restriction map for
pFN21AE3617
(Linear) (Six-base) MAPSORT of: KIEE3617.seq Check: 7690 from: 1 to: 1407
KIEE3617.orf
With 182 enzymes: SgfI *
December 27, 2011 16:36 ..
AatII G_ACGT'C
Cuts at: 0 341 1407
Size: 341 1066
Acc65I G'GTAC_C
Cuts at: 0 1070 1076 1407
Size: 1070 6 331
Fragments arranged by size:
1070 331 6
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 55 910 1282 1407
Size: 55 855 372 125
Fragments arranged by size:
855 372 125 55
AflIII A'CryG_T
Cuts at: 0 369 1407
Size: 369 1038
AgeI A'CCGG_T
Cuts at: 0 1067 1073 1407
Size: 1067 6 334
Fragments arranged by size:
1067 334 6
AlwNI CAG_nnn'CTG
Cuts at: 0 1116 1407
Size: 1116 291
ApoI r'AATT_y
Cuts at: 0 268 1016 1407
Size: 268 748 391
Fragments arranged by size:
748 391 268
AvaI C'yCGr_G
Cuts at: 0 209 401 827 1407
Size: 209 192 426 580
Fragments arranged by size:
580 426 209 192
BamHI G'GATC_C
Cuts at: 0 480 1407
Size: 480 927
BanI G'GyrC_C
Cuts at: 0 1070 1076 1392 1407
Size: 1070 6 316 15
Fragments arranged by size:
1070 316 15 6
BanII G_rGCy'C
Cuts at: 0 785 1407
Size: 785 622
BbvCI CC'TCA_GC
Cuts at: 0 932 1407
Size: 932 475
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 964 998 1407
Size: 964 34 409
Fragments arranged by size:
964 409 34
BglI GCCn_nnn'nGGC
Cuts at: 0 803 1407
Size: 803 604
BglII A'GATC_T
Cuts at: 0 41 1407
Size: 41 1366
Bme1580I G_kGCm'C
Cuts at: 0 206 321 950 1407
Size: 206 115 629 457
Fragments arranged by size:
629 457 206 115
BmgBI CAC'GTC
Cuts at: 0 243 1407
Size: 243 1164
BmrI ACTGGGnnnn_n'
Cuts at: 0 529 852 949 1407
Size: 529 323 97 458
Fragments arranged by size:
529 458 323 97
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1057 1138 1159 1355 1407
Size: 1057 81 21 196 52
Fragments arranged by size:
1057 196 81 52 21
Bpu10I CC'TnA_GC
Cuts at: 0 932 1407
Size: 932 475
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 775 1222 1347 1407
Size: 775 447 125 60
Fragments arranged by size:
775 447 125 60
BsaAI yAC'GTr
Cuts at: 0 675 738 1407
Size: 675 63 669
Fragments arranged by size:
675 669 63
BsaBI GATnn'nnATC
Cuts at: 0 1028 1407
Size: 1028 379
BsaHI Gr'CG_yC
Cuts at: 0 4 338 989 1407
Size: 4 334 651 418
Fragments arranged by size:
651 418 334 4
BsaWI w'CCGG_w
Cuts at: 0 1067 1073 1407
Size: 1067 6 334
Fragments arranged by size:
1067 334 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 373 1407
Size: 373 1034
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 61 1234 1407
Size: 61 1173 173
Fragments arranged by size:
1173 173 61
BsiHKAI G_wGCw'C
Cuts at: 0 785 1407
Size: 785 622
Bsp1286I G_dGCh'C
Cuts at: 0 206 321 785 950 1407
Size: 206 115 464 165 457
Fragments arranged by size:
464 457 206 165 115
BspHI T'CATG_A
Cuts at: 0 572 725 1407
Size: 572 153 682
Fragments arranged by size:
682 572 153
BsrBI CCG'CTC
Cuts at: 0 73 749 1407
Size: 73 676 658
Fragments arranged by size:
676 658 73
BsrDI GCAATG_nn'
Cuts at: 0 66 1407
Size: 66 1341
BsrFI r'CCGG_y
Cuts at: 0 764 1067 1073 1407
Size: 764 303 6 334
Fragments arranged by size:
764 334 303 6
BstAPI GCAn_nnn'nTGC
Cuts at: 0 587 1407
Size: 587 820
BstXI CCAn_nnnn'nTGG
Cuts at: 0 477 1407
Size: 477 930
BstYI r'GATC_y
Cuts at: 0 41 480 885 1407
Size: 41 439 405 522
Fragments arranged by size:
522 439 405 41
Bsu36I CC'TnA_GG
Cuts at: 0 485 1407
Size: 485 922
BtgI C'CryG_G
Cuts at: 0 10 1407
Size: 10 1397
BtsI GCAGTG_nn'
Cuts at: 0 319 1407
Size: 319 1088
ClaI AT'CG_AT
Cuts at: 0 980 1407
Size: 980 427
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 34 1407
Size: 34 1373
DraIII CAC_nnn'GTG
Cuts at: 0 943 1407
Size: 943 464
EaeI y'GGCC_r
Cuts at: 0 225 1407
Size: 225 1182
EarI CTCTTCn'nnn_
Cuts at: 0 284 907 1407
Size: 284 623 500
Fragments arranged by size:
623 500 284
EcoICRI GAG'CTC
Cuts at: 0 783 1407
Size: 783 624
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 55 910 1057 1138 1159 1282 1355 1407
Size: 55 855 147 81 21 123 73 52
Fragments arranged by size:
855 147 123 81 73 55 52 21
EcoNI CCTnn'n_nnAGG
Cuts at: 0 355 1210 1407
Size: 355 855 197
Fragments arranged by size:
855 355 197
EcoO109I rG'GnC_Cy
Cuts at: 0 294 621 633 810 966 1101 1407
Size: 294 327 12 177 156 135 306
Fragments arranged by size:
327 306 294 177 156 135 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 404 436 1407
Size: 404 32 971
Fragments arranged by size:
971 404 32
HincII GTy'rAC
Cuts at: 0 540 1407
Size: 540 867
KpnI G_GTAC'C
Cuts at: 0 1074 1080 1407
Size: 1074 6 327
Fragments arranged by size:
1074 327 6
MscI TGG'CCA
Cuts at: 0 227 1407
Size: 227 1180
MslI CAynn'nnrTG
Cuts at: 0 127 475 511 658 736 1407
Size: 127 348 36 147 78 671
Fragments arranged by size:
671 348 147 127 78 36
MspA1I CmG'CkG
Cuts at: 0 1227 1248 1407
Size: 1227 21 159
Fragments arranged by size:
1227 159 21
NaeI GCC'GGC
Cuts at: 0 766 1407
Size: 766 641
NdeI CA'TA_TG
Cuts at: 0 1281 1407
Size: 1281 126
NgoMIV G'CCGG_C
Cuts at: 0 764 1407
Size: 764 643
NspI r_CATG'y
Cuts at: 0 373 1407
Size: 373 1034
PciI A'CATG_T
Cuts at: 0 369 1407
Size: 369 1038
PflMI CCAn_nnn'nTGG
Cuts at: 0 1116 1407
Size: 1116 291
PmlI CAC'GTG
Cuts at: 0 738 1407
Size: 738 669
PpuMI rG'GwC_Cy
Cuts at: 0 621 633 810 966 1101 1407
Size: 621 12 177 156 135 306
Fragments arranged by size:
621 306 177 156 135 12
PvuII CAG'CTG
Cuts at: 0 1227 1407
Size: 1227 180
SacI G_AGCT'C
Cuts at: 0 785 1407
Size: 785 622
SanDI GG'GwC_CC
Cuts at: 0 810 1407
Size: 810 597
SexAI A'CCwGG_T
Cuts at: 0 1103 1407
Size: 1103 304
SmlI C'TyrA_G
Cuts at: 0 790 1237 1362 1407
Size: 790 447 125 45
Fragments arranged by size:
790 447 125 45
StuI AGG'CCT
Cuts at: 0 1344 1407
Size: 1344 63
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 931 1407
Size: 931 476
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 300 332 1407
Size: 300 32 1075
Fragments arranged by size:
1075 300 32
Tth111I GACn'n_nGTC
Cuts at: 0 244 535 1105 1407
Size: 244 291 570 302
Fragments arranged by size:
570 302 291 244
XmnI GAAnn'nnTTC
Cuts at: 0 272 1407
Size: 272 1135
ZraI GAC'GTC
Cuts at: 0 339 1407
Size: 339 1068
Enzymes that do cut:
AatII Acc65I AcuI AflIII AgeI AlwNI ApoI AvaI
BamHI BanI BanII BbvCI BcgI BglI BglII Bme1580I
BmgBI BmrI BpmI Bpu10I BpuEI BsaAI BsaBI BsaHI
BsaWI BseRI BsgI BsiHKAI Bsp1286I BspHI BsrBI BsrDI
BsrFI BstAPI BstXI BstYI Bsu36I BtgI BtsI ClaI
CspCI DraIII EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I
Hin4I HincII KpnI MscI MslI MspA1I NaeI NdeI
NgoMIV NspI PciI PflMI PmlI PpuMI PvuII SacI
SanDI SexAI SmlI StuI TaqII TstI Tth111I XmnI
ZraI
Enzymes that do not cut:
AarI AccI AclI AfeI AflII AhdI AleI AlfI
AloI ApaI ApaLI AscI AseI AsiSI AvrII BaeI
BbeI BbsI BciVI BclI BfrBI BlpI BmtI BplI
BsaI BsaXI BseYI BsiEI BsiWI BsmI BsmBI BspEI
BspMI BsrGI BssHII BssSI BstBI BstEII BstZ17I BtgZI
DraI DrdI EagI EciI EcoRI EcoRV FalI FseI
FspI FspAI HaeII HindIII HpaI KasI MfeI MluI
MmeI NarI NcoI NheI NotI NruI NsiI PacI
PasI PfoI PmeI PpiI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI RsrII SacII SalI SapI SbfI
ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI StyI SwaI TatI XbaI
XcmI XhoI XmaI