(Linear) (Six-base) MAPSORT of: KIEE3612.seq  Check: 9651  from: 1  to: 345



KIEE3612.orf

 With 182 enzymes: SgfI * 

                        March 22, 2012 16:37  ..

AlwNI CAG_nnn'CTG

Cuts at:      0     255     345 
   Size:        255      90
 
AvaI C'yCGr_G

Cuts at:      0     161     345 
   Size:        161     184
 
BanI G'GyrC_C

Cuts at:      0      49      66     114     175     345 
   Size:         49      17      48      61     170

  Fragments arranged by size: 

                 170      61      49      48      17
 
BanII G_rGCy'C

Cuts at:      0      91     345 
   Size:         91     254
 
BbeI G_GCGC'C

Cuts at:      0      53     118     179     345 
   Size:         53      65      61     166

  Fragments arranged by size: 

                 166      65      61      53
 
BbvCI CC'TCA_GC

Cuts at:      0     130     345 
   Size:        130     215
 
BglI GCCn_nnn'nGGC

Cuts at:      0     123     184     345 
   Size:        123      61     161

  Fragments arranged by size: 

                 161     123      61
 
Bpu10I CC'TnA_GC

Cuts at:      0     130     345 
   Size:        130     215
 
BsaHI Gr'CG_yC

Cuts at:      0      50     115     176     345 
   Size:         50      65      61     169

  Fragments arranged by size: 

                 169      65      61      50
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     319     345 
   Size:        319      26
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     124     340     345 
   Size:        124     216       5

  Fragments arranged by size: 

                 216     124       5
 
BseYI C'CCAG_C

Cuts at:      0     262     345 
   Size:        262      83
 
Bsp1286I G_dGCh'C

Cuts at:      0      91     345 
   Size:         91     254
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     254     345 
   Size:        254      91
 
BsrFI r'CCGG_y

Cuts at:      0      63     345 
   Size:         63     282
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      66     345 
   Size:         66     279
 
BstYI r'GATC_y

Cuts at:      0      78     345 
   Size:         78     267
 
BtgI C'CryG_G

Cuts at:      0      83     345 
   Size:         83     262
 
BtsI GCAGTG_nn'

Cuts at:      0     324     345 
   Size:        324      21
 
EaeI y'GGCC_r

Cuts at:      0     309     345 
   Size:        309      36
 
HaeII r_GCGC'y

Cuts at:      0      53     118     179     345 
   Size:         53      65      61     166

  Fragments arranged by size: 

                 166      65      61      53
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     142     174     345 
   Size:        142      32     171

  Fragments arranged by size: 

                 171     142      32
 
KasI G'GCGC_C

Cuts at:      0      49     114     175     345 
   Size:         49      65      61     170

  Fragments arranged by size: 

                 170      65      61      49
 
MspA1I CmG'CkG

Cuts at:      0       8      85     102     129     345 
   Size:          8      77      17      27     216

  Fragments arranged by size: 

                 216      77      27      17       8
 
NarI GG'CG_CC

Cuts at:      0      50     115     176     345 
   Size:         50      65      61     169

  Fragments arranged by size: 

                 169      65      61      50
 
PshAI GACnn'nnGTC

Cuts at:      0     228     345 
   Size:        228     117
 
PstI C_TGCA'G

Cuts at:      0     101     251     345 
   Size:        101     150      94

  Fragments arranged by size: 

                 150     101      94
 
PvuII CAG'CTG

Cuts at:      0     129     345 
   Size:        129     216
 
SacII CC_GC'GG

Cuts at:      0      86     345 
   Size:         86     259
 
SfcI C'TryA_G

Cuts at:      0      97     247     345 
   Size:         97     150      98

  Fragments arranged by size: 

                 150      98      97
 
SfoI GGC'GCC

Cuts at:      0      51     116     177     345 
   Size:         51      65      61     168

  Fragments arranged by size: 

                 168      65      61      51
 

 Enzymes that do cut:

    AlwNI     AvaI     BanI    BanII     BbeI    BbvCI     BglI   Bpu10I
    BsaHI    BsaXI    BseRI    BseYI Bsp1286I    BspMI    BsrFI   BstAPI
    BstYI     BtgI     BtsI     EaeI    HaeII    Hin4I     KasI   MspA1I
     NarI    PshAI     PstI    PvuII    SacII     SfcI     SfoI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BbsI
     BcgI    BciVI     BclI    BfrBI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BmtI     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI
    BsaWI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI
    BspHI    BsrBI    BsrDI    BsrGI   BssHII    BssSI    BstBI   BstEII
    BstXI  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI   DraIII
     DrdI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI   HincII  HindIII
     HpaI     KpnI     MfeI     MluI     MmeI     MscI     MslI     NaeI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PpuMI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     StyI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     XmnI     ZraI