(Linear) (Six-base) MAPSORT of: KIEE3611.seq  Check: 7085  from: 1  to: 285



KIEE3611.orf

 With 182 enzymes: SgfI * 

                        December 27, 2011 16:36  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0      52     285 
   Size:         52     233
 
AleI CACnn'nnGTG

Cuts at:      0      46     285 
   Size:         46     239
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      33     285 
   Size:         33     252
 
AlwNI CAG_nnn'CTG

Cuts at:      0      69     285 
   Size:         69     216
 
AvrII C'CTAG_G

Cuts at:      0     187     285 
   Size:        187      98
 
BanI G'GyrC_C

Cuts at:      0      76     285 
   Size:         76     209
 
BanII G_rGCy'C

Cuts at:      0      90     269     285 
   Size:         90     179      16

  Fragments arranged by size: 

                 179      90      16
 
BlpI GC'TnA_GC

Cuts at:      0     268     285 
   Size:        268      17
 
Bme1580I G_kGCm'C

Cuts at:      0      81     285 
   Size:         81     204
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     135     285 
   Size:        135     150
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     115     285 
   Size:        115     170
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      88     118     223     253     285 
   Size:         88      30     105      30      32

  Fragments arranged by size: 

                 105      88      32      30      30
 
BseYI C'CCAG_C

Cuts at:      0      72     285 
   Size:         72     213
 
BsiHKAI G_wGCw'C

Cuts at:      0     269     285 
   Size:        269      16
 
Bsp1286I G_dGCh'C

Cuts at:      0      81      90     269     285 
   Size:         81       9     179      16

  Fragments arranged by size: 

                 179      81      16       9
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      52     285 
   Size:         52     233
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      48     285 
   Size:         48     237
 
BtsI GCAGTG_nn'

Cuts at:      0      67     285 
   Size:         67     218
 
EaeI y'GGCC_r

Cuts at:      0       2     285 
   Size:          2     283
 
EcoICRI GAG'CTC

Cuts at:      0     267     285 
   Size:        267      18
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     115     285 
   Size:        115     170
 
EcoO109I rG'GnC_Cy

Cuts at:      0     239     285 
   Size:        239      46
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     223     255     285 
   Size:        223      32      30

  Fragments arranged by size: 

                 223      32      30
 
MslI CAynn'nnrTG

Cuts at:      0      46     172     285 
   Size:         46     126     113

  Fragments arranged by size: 

                 126     113      46
 
MspA1I CmG'CkG

Cuts at:      0     151     276     285 
   Size:        151     125       9

  Fragments arranged by size: 

                 151     125       9
 
NspI r_CATG'y

Cuts at:      0     177     250     285 
   Size:        177      73      35

  Fragments arranged by size: 

                 177      73      35
 
PasI CC'CwG_GG

Cuts at:      0      81     109     260     285 
   Size:         81      28     151      25

  Fragments arranged by size: 

                 151      81      28      25
 
PpuMI rG'GwC_Cy

Cuts at:      0     239     285 
   Size:        239      46
 
PvuII CAG'CTG

Cuts at:      0     151     285 
   Size:        151     134
 
SacI G_AGCT'C

Cuts at:      0     269     285 
   Size:        269      16
 
SanDI GG'GwC_CC

Cuts at:      0     239     285 
   Size:        239      46
 
SfcI C'TryA_G

Cuts at:      0      61     285 
   Size:         61     224
 
SphI G_CATG'C

Cuts at:      0     250     285 
   Size:        250      35
 
StuI AGG'CCT

Cuts at:      0     186     285 
   Size:        186      99
 
StyI C'CwwG_G

Cuts at:      0     187     285 
   Size:        187      98
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      64     285 
   Size:         64     221
 

 Enzymes that do cut:

     AarI     AleI     AlfI    AlwNI    AvrII     BanI    BanII     BlpI
 Bme1580I     BmrI     BpmI    BsaXI    BseYI  BsiHKAI Bsp1286I    BspMI
    BstXI     BtsI     EaeI  EcoICRI  Eco57MI EcoO109I    Hin4I     MslI
   MspA1I     NspI     PasI    PpuMI    PvuII     SacI    SanDI     SfcI
     SphI     StuI     StyI    TaqII

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AloI     ApaI    ApaLI     ApoI     AscI     AseI
    AsiSI     AvaI     BaeI    BamHI     BbeI     BbsI    BbvCI     BcgI
    BciVI     BclI    BfrBI     BglI    BglII    BmgBI     BmtI     BplI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BseRI
     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI     DraI
   DraIII     DrdI     EagI     EarI     EciI    EcoNI    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MscI     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII    SacII     SalI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SrfI     SspI     SwaI     TatI
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI