Restriction map for
pFN21AE3610
(Linear) (Six-base) MAPSORT of: KIEE3610.seq Check: 3136 from: 1 to: 1092
KIEE3610.orf
With 182 enzymes: SgfI *
December 27, 2011 16:36 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 674 1092
Size: 674 418
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 891 1092
Size: 891 201
ApaI G_GGCC'C
Cuts at: 0 398 1092
Size: 398 694
ApoI r'AATT_y
Cuts at: 0 158 290 588 642 1092
Size: 158 132 298 54 450
Fragments arranged by size:
450 298 158 132 54
AvaI C'yCGr_G
Cuts at: 0 200 1092
Size: 200 892
BanI G'GyrC_C
Cuts at: 0 111 1092
Size: 111 981
BanII G_rGCy'C
Cuts at: 0 398 1092
Size: 398 694
BbeI G_GCGC'C
Cuts at: 0 115 1092
Size: 115 977
BbsI GAAGACnn'nnnn_
Cuts at: 0 662 1067 1092
Size: 662 405 25
Fragments arranged by size:
662 405 25
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 191 225 1092
Size: 191 34 867
Fragments arranged by size:
867 191 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 997 1092
Size: 997 95
BclI T'GATC_A
Cuts at: 0 720 1092
Size: 720 372
BfrBI ATG'CAT
Cuts at: 0 951 1092
Size: 951 141
Bme1580I G_kGCm'C
Cuts at: 0 398 1092
Size: 398 694
BmrI ACTGGGnnnn_n'
Cuts at: 0 200 1092
Size: 200 892
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 625 1092
Size: 625 467
Bpu10I CC'TnA_GC
Cuts at: 0 482 560 1092
Size: 482 78 532
Fragments arranged by size:
532 482 78
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 618 1092
Size: 618 474
BsaI GGTCTCn'nnnn_
Cuts at: 0 201 1092
Size: 201 891
BsaAI yAC'GTr
Cuts at: 0 216 1092
Size: 216 876
BsaHI Gr'CG_yC
Cuts at: 0 112 230 1092
Size: 112 118 862
Fragments arranged by size:
862 118 112
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 313 1092
Size: 313 779
BseYI C'CCAG_C
Cuts at: 0 225 374 378 1092
Size: 225 149 4 714
Fragments arranged by size:
714 225 149 4
Bsp1286I G_dGCh'C
Cuts at: 0 398 1092
Size: 398 694
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 554 1092
Size: 554 538
BsrDI GCAATG_nn'
Cuts at: 0 845 1092
Size: 845 247
BstYI r'GATC_y
Cuts at: 0 968 1092
Size: 968 124
BtgI C'CryG_G
Cuts at: 0 234 517 879 1092
Size: 234 283 362 213
Fragments arranged by size:
362 283 234 213
DraIII CAC_nnn'GTG
Cuts at: 0 529 1092
Size: 529 563
DrdI GACnn_nn'nnGTC
Cuts at: 0 1069 1092
Size: 1069 23
EaeI y'GGCC_r
Cuts at: 0 520 1092
Size: 520 572
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 625 674 1092
Size: 625 49 418
Fragments arranged by size:
625 418 49
EcoNI CCTnn'n_nnAGG
Cuts at: 0 448 485 1092
Size: 448 37 607
Fragments arranged by size:
607 448 37
EcoO109I rG'GnC_Cy
Cuts at: 0 394 822 1092
Size: 394 428 270
Fragments arranged by size:
428 394 270
HaeII r_GCGC'y
Cuts at: 0 115 1092
Size: 115 977
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 616 648 740 772 894 926 1092
Size: 616 32 92 32 122 32 166
Fragments arranged by size:
616 166 122 92 32 32 32
HincII GTy'rAC
Cuts at: 0 365 960 1092
Size: 365 595 132
Fragments arranged by size:
595 365 132
HindIII A'AGCT_T
Cuts at: 0 299 769 1092
Size: 299 470 323
Fragments arranged by size:
470 323 299
KasI G'GCGC_C
Cuts at: 0 111 1092
Size: 111 981
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 156 1092
Size: 156 936
MscI TGG'CCA
Cuts at: 0 522 1092
Size: 522 570
MslI CAynn'nnrTG
Cuts at: 0 1012 1092
Size: 1012 80
MspA1I CmG'CkG
Cuts at: 0 236 378 1092
Size: 236 142 714
Fragments arranged by size:
714 236 142
NarI GG'CG_CC
Cuts at: 0 112 1092
Size: 112 980
NcoI C'CATG_G
Cuts at: 0 517 879 1092
Size: 517 362 213
Fragments arranged by size:
517 362 213
NruI TCG'CGA
Cuts at: 0 173 1092
Size: 173 919
NsiI A_TGCA'T
Cuts at: 0 953 1092
Size: 953 139
NspI r_CATG'y
Cuts at: 0 955 1092
Size: 955 137
PflMI CCAn_nnn'nTGG
Cuts at: 0 96 1092
Size: 96 996
PfoI T'CCnGG_A
Cuts at: 0 164 602 818 1092
Size: 164 438 216 274
Fragments arranged by size:
438 274 216 164
PpuMI rG'GwC_Cy
Cuts at: 0 822 1092
Size: 822 270
PspOMI G'GGCC_C
Cuts at: 0 394 1092
Size: 394 698
PstI C_TGCA'G
Cuts at: 0 317 1092
Size: 317 775
PvuII CAG'CTG
Cuts at: 0 378 1092
Size: 378 714
SacII CC_GC'GG
Cuts at: 0 237 1092
Size: 237 855
SfcI C'TryA_G
Cuts at: 0 313 1092
Size: 313 779
SfoI GGC'GCC
Cuts at: 0 113 1092
Size: 113 979
SmlI C'TyrA_G
Cuts at: 0 633 1092
Size: 633 459
SnaBI TAC'GTA
Cuts at: 0 216 1092
Size: 216 876
SphI G_CATG'C
Cuts at: 0 955 1092
Size: 955 137
StyI C'CwwG_G
Cuts at: 0 264 517 854 879 1092
Size: 264 253 337 25 213
Fragments arranged by size:
337 264 253 213 25
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1073 1092
Size: 1073 19
TatI w'GTAC_w
Cuts at: 0 243 1092
Size: 243 849
Tth111I GACn'n_nGTC
Cuts at: 0 1039 1092
Size: 1039 53
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 861 1092
Size: 861 231
XmnI GAAnn'nnTTC
Cuts at: 0 160 589 1092
Size: 160 429 503
Fragments arranged by size:
503 429 160
Enzymes that do cut:
AcuI AlfI ApaI ApoI AvaI BanI BanII BbeI
BbsI BcgI BciVI BclI BfrBI Bme1580I BmrI BpmI
Bpu10I BpuEI BsaI BsaAI BsaHI BseRI BseYI Bsp1286I
BspMI BsrDI BstYI BtgI DraIII DrdI EaeI Eco57MI
EcoNI EcoO109I HaeII Hin4I HincII HindIII KasI MmeI
MscI MslI MspA1I NarI NcoI NruI NsiI NspI
PflMI PfoI PpuMI PspOMI PstI PvuII SacII SfcI
SfoI SmlI SnaBI SphI StyI TaqII TatI Tth111I
XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AloI AlwNI ApaLI AscI AseI
AsiSI AvrII BaeI BamHI BbvCI BglI BglII BlpI
BmgBI BmtI BplI BsaBI BsaWI BsaXI BsgI BsiEI
BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I
Bsu36I BtgZI BtsI ClaI CspCI DraI EagI EarI
EciI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI
HpaI KpnI MfeI MluI NaeI NdeI NgoMIV NheI
NotI PacI PasI PciI PmeI PmlI PpiI PshAI
PsiI PspXI PsrI PvuI RsrII SacI SalI SanDI
SapI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI
SpeI SrfI SspI StuI SwaI TstI XbaI XhoI
XmaI ZraI