Restriction map for
pFN21AE3609
(Linear) (Six-base) MAPSORT of: KIEE3609.seq Check: 1602 from: 1 to: 1035
KIEE3609.orf
With 182 enzymes: SgfI *
July 3, 2012 18:12 ..
AatII G_ACGT'C
Cuts at: 0 7 1035
Size: 7 1028
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 143 316 332 1035
Size: 143 173 16 703
Fragments arranged by size:
703 173 143 16
AhdI GACnn_n'nnGTC
Cuts at: 0 393 1035
Size: 393 642
AlwNI CAG_nnn'CTG
Cuts at: 0 679 1035
Size: 679 356
ApoI r'AATT_y
Cuts at: 0 163 201 280 1035
Size: 163 38 79 755
Fragments arranged by size:
755 163 79 38
BbsI GAAGACnn'nnnn_
Cuts at: 0 131 1035
Size: 131 904
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 163 197 1035
Size: 163 34 838
Fragments arranged by size:
838 163 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 967 1035
Size: 967 68
BfrBI ATG'CAT
Cuts at: 0 266 377 901 1035
Size: 266 111 524 134
Fragments arranged by size:
524 266 134 111
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 475 914 1035
Size: 475 439 121
Fragments arranged by size:
475 439 121
BsaI GGTCTCn'nnnn_
Cuts at: 0 936 1035
Size: 936 99
BsaHI Gr'CG_yC
Cuts at: 0 4 1035
Size: 4 1031
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 578 990 1035
Size: 578 412 45
Fragments arranged by size:
578 412 45
BstYI r'GATC_y
Cuts at: 0 290 1035
Size: 290 745
BtsI GCAGTG_nn'
Cuts at: 0 836 1035
Size: 836 199
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 194 229 1035
Size: 194 35 806
Fragments arranged by size:
806 194 35
EaeI y'GGCC_r
Cuts at: 0 966 1035
Size: 966 69
EarI CTCTTCn'nnn_
Cuts at: 0 292 1035
Size: 292 743
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 143 316 332 475 914 1035
Size: 143 173 16 143 439 121
Fragments arranged by size:
439 173 143 143 121 16
EcoO109I rG'GnC_Cy
Cuts at: 0 395 1035
Size: 395 640
EcoRI G'AATT_C
Cuts at: 0 201 1035
Size: 201 834
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 97 1035
Size: 97 938
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 379 411 1035
Size: 379 32 624
Fragments arranged by size:
624 379 32
MfeI C'AATT_G
Cuts at: 0 423 1035
Size: 423 612
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 888 1035
Size: 888 147
MscI TGG'CCA
Cuts at: 0 968 1035
Size: 968 67
MslI CAynn'nnrTG
Cuts at: 0 185 323 814 1035
Size: 185 138 491 221
Fragments arranged by size:
491 221 185 138
NsiI A_TGCA'T
Cuts at: 0 268 379 903 1035
Size: 268 111 524 132
Fragments arranged by size:
524 268 132 111
NspI r_CATG'y
Cuts at: 0 377 1035
Size: 377 658
PflMI CCAn_nnn'nTGG
Cuts at: 0 117 1035
Size: 117 918
PpuMI rG'GwC_Cy
Cuts at: 0 395 1035
Size: 395 640
PshAI GACnn'nnGTC
Cuts at: 0 7 1035
Size: 7 1028
PsiI TTA'TAA
Cuts at: 0 431 1035
Size: 431 604
SfcI C'TryA_G
Cuts at: 0 62 821 1035
Size: 62 759 214
Fragments arranged by size:
759 214 62
SphI G_CATG'C
Cuts at: 0 377 1035
Size: 377 658
SspI AAT'ATT
Cuts at: 0 21 1035
Size: 21 1014
StyI C'CwwG_G
Cuts at: 0 795 1035
Size: 795 240
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 808 1035
Size: 808 227
XmnI GAAnn'nnTTC
Cuts at: 0 20 163 1035
Size: 20 143 872
Fragments arranged by size:
872 143 20
ZraI GAC'GTC
Cuts at: 0 5 1035
Size: 5 1030
Enzymes that do cut:
AatII AcuI AhdI AlwNI ApoI BbsI BcgI BciVI
BfrBI BpmI BsaI BsaHI BsgI BstYI BtsI CspCI
EaeI EarI Eco57MI EcoO109I EcoRI FalI Hin4I MfeI
MmeI MscI MslI NsiI NspI PflMI PpuMI PshAI
PsiI SfcI SphI SspI StyI TaqII XmnI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AflIII AgeI
AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BanII BbeI BbvCI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI Bpu10I BpuEI BsaAI BsaBI BsaWI BsaXI BseRI
BseYI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgI BtgZI
ClaI DraI DraIII DrdI EagI EciI EcoICRI EcoNI
EcoRV FseI FspI FspAI HaeII HincII HindIII HpaI
KasI KpnI MluI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI PacI PasI PciI PfoI
PmeI PmlI PpiI PspOMI PspXI PsrI PstI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SrfI StuI SwaI TatI TstI Tth111I
XbaI XcmI XhoI XmaI