Restriction map for
pFN21AE3608
(Linear) (Six-base) MAPSORT of: KIEE3608.seq Check: 4011 from: 1 to: 3996
KIEE3608.orf
With 182 enzymes: SgfI *
March 22, 2012 16:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1175 3996
Size: 1175 2821
AatII G_ACGT'C
Cuts at: 0 2612 3996
Size: 2612 1384
AccI GT'mk_AC
Cuts at: 0 1045 3500 3575 3704 3996
Size: 1045 2455 75 129 292
Fragments arranged by size:
2455 1045 292 129 75
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 394 871 1447 1786 1955 2696 3996
Size: 394 477 576 339 169 741 1300
Fragments arranged by size:
1300 741 576 477 394 339 169
AflII C'TTAA_G
Cuts at: 0 904 3996
Size: 904 3092
AflIII A'CryG_T
Cuts at: 0 802 3713 3996
Size: 802 2911 283
Fragments arranged by size:
2911 802 283
AhdI GACnn_n'nnGTC
Cuts at: 0 2469 2811 3996
Size: 2469 342 1185
Fragments arranged by size:
2469 1185 342
AleI CACnn'nnGTG
Cuts at: 0 2596 2656 3356 3996
Size: 2596 60 700 640
Fragments arranged by size:
2596 700 640 60
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 167 1433 3691 3996
Size: 167 1266 2258 305
Fragments arranged by size:
2258 1266 305 167
AlwNI CAG_nnn'CTG
Cuts at: 0 340 849 1122 1305 1488 1783 1803 3996
Size: 340 509 273 183 183 295 20 2193
Fragments arranged by size:
2193 509 340 295 273 183 183 20
ApaI G_GGCC'C
Cuts at: 0 1560 1673 2162 3012 3432 3996
Size: 1560 113 489 850 420 564
Fragments arranged by size:
1560 850 564 489 420 113
ApaLI G'TGCA_C
Cuts at: 0 3610 3996
Size: 3610 386
ApoI r'AATT_y
Cuts at: 0 1917 3448 3996
Size: 1917 1531 548
Fragments arranged by size:
1917 1531 548
AvaI C'yCGr_G
Cuts at: 0 282 1147 1559 1895 3005 3579 3996
Size: 282 865 412 336 1110 574 417
Fragments arranged by size:
1110 865 574 417 412 336 282
BanI G'GyrC_C
Cuts at: 0 1214 2001 2140 2267 2650 3996
Size: 1214 787 139 127 383 1346
Fragments arranged by size:
1346 1214 787 383 139 127
BanII G_rGCy'C
Cuts at: 0 280 690 723 1560 1646 1673 1721 2162
Size: 280 410 33 837 86 27 48 441
Cuts at: 2162 2848 3012 3206 3409 3432 3879 3996
Size: 686 164 194 203 23 447 117
Fragments arranged by size:
837 686 447 441 410 280 203 194
164 117 86 48 33 27 23
BbeI G_GCGC'C
Cuts at: 0 1218 3996
Size: 1218 2778
BbsI GAAGACnn'nnnn_
Cuts at: 0 3852 3996
Size: 3852 144
BbvCI CC'TCA_GC
Cuts at: 0 494 3996
Size: 494 3502
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2925 2959 3996
Size: 2925 34 1037
Fragments arranged by size:
2925 1037 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 2568 3996
Size: 2568 1428
BglI GCCn_nnn'nGGC
Cuts at: 0 2157 2391 3996
Size: 2157 234 1605
Fragments arranged by size:
2157 1605 234
BlpI GC'TnA_GC
Cuts at: 0 527 2168 3996
Size: 527 1641 1828
Fragments arranged by size:
1828 1641 527
Bme1580I G_kGCm'C
Cuts at: 0 1560 1673 1896 2143 2162 2270 2938 3012
Size: 1560 113 223 247 19 108 668 74
Cuts at: 3012 3106 3353 3432 3614 3996
Size: 94 247 79 182 382
Fragments arranged by size:
1560 668 382 247 247 223 182 113
108 94 79 74 19
BmgBI CAC'GTC
Cuts at: 0 2733 2964 3996
Size: 2733 231 1032
Fragments arranged by size:
2733 1032 231
BmrI ACTGGGnnnn_n'
Cuts at: 0 1791 1883 3996
Size: 1791 92 2113
Fragments arranged by size:
2113 1791 92
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1035 3846 3996
Size: 1035 2811 150
Fragments arranged by size:
2811 1035 150
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 572 1057 1058 1309 1378 1387 2138 2311
Size: 572 485 1 251 69 9 751 173
Cuts at: 2311 2906 3217 3531 3996
Size: 595 311 314 465
Fragments arranged by size:
751 595 572 485 465 314 311 251
173 69 9 1
Bpu10I CC'TnA_GC
Cuts at: 0 494 823 1640 3996
Size: 494 329 817 2356
Fragments arranged by size:
2356 817 494 329
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 742 1411 3385 3514 3871 3996
Size: 742 669 1974 129 357 125
Fragments arranged by size:
1974 742 669 357 129 125
BsaI GGTCTCn'nnnn_
Cuts at: 0 656 1136 3013 3996
Size: 656 480 1877 983
Fragments arranged by size:
1877 983 656 480
BsaAI yAC'GTr
Cuts at: 0 3716 3996
Size: 3716 280
BsaHI Gr'CG_yC
Cuts at: 0 1215 2609 3996
Size: 1215 1394 1387
Fragments arranged by size:
1394 1387 1215
BsaWI w'CCGG_w
Cuts at: 0 3032 3269 3996
Size: 3032 237 727
Fragments arranged by size:
3032 727 237
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 3769 3799 3996
Size: 3769 30 197
Fragments arranged by size:
3769 197 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 199 964 994 1210 1222 1240 1330 1333
Size: 199 765 30 216 12 18 90 3
Cuts at: 1333 1384 1685 1913 2229 2304 2497 2524 2629
Size: 51 301 228 316 75 193 27 105
Cuts at: 2629 2695 3529 3996
Size: 66 834 467
Fragments arranged by size:
834 765 467 316 301 228 216 199
193 105 90 75 66 51 30 27
18 12 3
BseYI C'CCAG_C
Cuts at: 0 9 64 1839 1948 1962 2228 2630 2743
Size: 9 55 1775 109 14 266 402 113
Cuts at: 2743 3803 3996
Size: 1060 193
Fragments arranged by size:
1775 1060 402 266 193 113 109 55
14 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1156 3092 3268 3996
Size: 1156 1936 176 728
Fragments arranged by size:
1936 1156 728 176
BsiHKAI G_wGCw'C
Cuts at: 0 528 2342 2848 3409 3614 3879 3996
Size: 528 1814 506 561 205 265 117
Fragments arranged by size:
1814 561 528 506 265 205 117
BsiWI C'GTAC_G
Cuts at: 0 3716 3996
Size: 3716 280
BsmI GAATG_Cn'
Cuts at: 0 495 3996
Size: 495 3501
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1396 3832 3996
Size: 1396 2436 164
Fragments arranged by size:
2436 1396 164
Bsp1286I G_dGCh'C
Cuts at: 0 280 528 690 723 1560 1646 1673 1721
Size: 280 248 162 33 837 86 27 48
Cuts at: 1721 1896 2143 2162 2270 2342 2848 2938 3012
Size: 175 247 19 108 72 506 90 74
Cuts at: 3012 3106 3206 3353 3409 3432 3614 3879 3996
Size: 94 100 147 56 23 182 265 117
Fragments arranged by size:
837 506 280 265 248 247 182 175
162 147 117 108 100 94 90 86
74 72 56 48 33 27 23 19
BspEI T'CCGG_A
Cuts at: 0 3032 3269 3996
Size: 3032 237 727
Fragments arranged by size:
3032 727 237
BspHI T'CATG_A
Cuts at: 0 1025 3962 3986 3996
Size: 1025 2937 24 10
Fragments arranged by size:
2937 1025 24 10
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 879 1175 2699 2732 3101 3218 3996
Size: 879 296 1524 33 369 117 778
Fragments arranged by size:
1524 879 778 369 296 117 33
BsrBI CCG'CTC
Cuts at: 0 885 1212 1245 1323 2136 2295 2378 2421
Size: 885 327 33 78 813 159 83 43
Cuts at: 2421 2685 3375 3830 3903 3935 3996
Size: 264 690 455 73 32 61
Fragments arranged by size:
885 813 690 455 327 264 159 83
78 73 61 43 33 32
BsrDI GCAATG_nn'
Cuts at: 0 102 2715 3996
Size: 102 2613 1281
Fragments arranged by size:
2613 1281 102
BsrFI r'CCGG_y
Cuts at: 0 1710 2390 3996
Size: 1710 680 1606
Fragments arranged by size:
1710 1606 680
BsrGI T'GTAC_A
Cuts at: 0 116 3996
Size: 116 3880
BssSI C'ACGA_G
Cuts at: 0 2816 3996
Size: 2816 1180
BstAPI GCAn_nnn'nTGC
Cuts at: 0 474 714 1122 1305 1488 1879 2080 3996
Size: 474 240 408 183 183 391 201 1916
Fragments arranged by size:
1916 474 408 391 240 201 183 183
BstXI CCAn_nnnn'nTGG
Cuts at: 0 675 2818 3996
Size: 675 2143 1178
Fragments arranged by size:
2143 1178 675
BstYI r'GATC_y
Cuts at: 0 68 407 2865 2873 3029 3062 3824 3996
Size: 68 339 2458 8 156 33 762 172
Fragments arranged by size:
2458 762 339 172 156 68 33 8
Bsu36I CC'TnA_GG
Cuts at: 0 412 2209 3996
Size: 412 1797 1787
Fragments arranged by size:
1797 1787 412
BtgI C'CryG_G
Cuts at: 0 542 836 878 996 2459 2594 2654 3326
Size: 542 294 42 118 1463 135 60 672
Cuts at: 3326 3410 3996
Size: 84 586
Fragments arranged by size:
1463 672 586 542 294 135 118 84
60 42
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 228 582 2582 3378 3996
Size: 228 354 2000 796 618
Fragments arranged by size:
2000 796 618 354 228
BtsI GCAGTG_nn'
Cuts at: 0 1877 2027 2502 2898 3754 3996
Size: 1877 150 475 396 856 242
Fragments arranged by size:
1877 856 475 396 242 150
DraI TTT'AAA
Cuts at: 0 1106 3996
Size: 1106 2890
DraIII CAC_nnn'GTG
Cuts at: 0 444 3996
Size: 444 3552
DrdI GACnn_nn'nnGTC
Cuts at: 0 3700 3996
Size: 3700 296
EaeI y'GGCC_r
Cuts at: 0 1610 2062 2383 3996
Size: 1610 452 321 1613
Fragments arranged by size:
1613 1610 452 321
EarI CTCTTCn'nnn_
Cuts at: 0 185 268 730 1565 2672 3996
Size: 185 83 462 835 1107 1324
Fragments arranged by size:
1324 1107 835 462 185 83
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 36 737 1348 1900 1963 2091 3996
Size: 36 701 611 552 63 128 1905
Fragments arranged by size:
1905 701 611 552 128 63 36
EcoICRI GAG'CTC
Cuts at: 0 2846 3407 3877 3996
Size: 2846 561 470 119
Fragments arranged by size:
2846 561 470 119
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 394 572 871 1057 1058 1309 1378 1387
Size: 394 178 299 186 1 251 69 9
Cuts at: 1387 1447 1786 1955 2138 2311 2696 2906 3217
Size: 60 339 169 183 173 385 210 311
Cuts at: 3217 3531 3996
Size: 314 465
Fragments arranged by size:
465 394 385 339 314 311 299 251
210 186 183 178 173 169 69 60
9 1
EcoNI CCTnn'n_nnAGG
Cuts at: 0 376 2275 3996
Size: 376 1899 1721
Fragments arranged by size:
1899 1721 376
EcoO109I rG'GnC_Cy
Cuts at: 0 57 1636 1669 1670 1678 1758 1939 1989
Size: 57 1579 33 1 8 80 181 50
Cuts at: 1989 2012 2057 2158 2159 2436 2627 2832 3429
Size: 23 45 101 1 277 191 205 597
Cuts at: 3429 3996
Size: 567
Fragments arranged by size:
1579 597 567 277 205 191 181 101
80 57 50 45 33 23 8 1
1
EcoRI G'AATT_C
Cuts at: 0 1917 3996
Size: 1917 2079
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 876 3996
Size: 876 3120
FseI GG_CCGG'CC
Cuts at: 0 2394 3996
Size: 2394 1602
HaeII r_GCGC'y
Cuts at: 0 1218 3996
Size: 1218 2778
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 251 283 634 666 1889 1892 1915 1921
Size: 251 32 351 32 1223 3 23 6
Cuts at: 1921 1924 1947 2362 2394 2455 2487 2797 2829
Size: 3 23 415 32 61 32 310 32
Cuts at: 2829 3562 3594 3996
Size: 733 32 402
Fragments arranged by size:
1223 733 415 402 351 310 251 61
32 32 32 32 32 32 23 23
6 3 3
HincII GTy'rAC
Cuts at: 0 1046 2985 3090 3501 3576 3711 3996
Size: 1046 1939 105 411 75 135 285
Fragments arranged by size:
1939 1046 411 285 135 105 75
KasI G'GCGC_C
Cuts at: 0 1214 3996
Size: 1214 2782
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 3812 3996
Size: 3812 184
MscI TGG'CCA
Cuts at: 0 1612 3996
Size: 1612 2384
MslI CAynn'nnrTG
Cuts at: 0 431 673 1030 2596 2656 3356 3607 3673
Size: 431 242 357 1566 60 700 251 66
Cuts at: 3673 3996
Size: 323
Fragments arranged by size:
1566 700 431 357 323 251 242 66
60
MspA1I CmG'CkG
Cuts at: 0 147 531 971 1122 1179 1185 1236 1254
Size: 147 384 440 151 57 6 51 18
Cuts at: 1254 1281 1365 1377 1404 1540 2952 3525 3996
Size: 27 84 12 27 136 1412 573 471
Fragments arranged by size:
1412 573 471 440 384 151 147 136
84 57 51 27 27 18 12 6
NaeI GCC'GGC
Cuts at: 0 2392 3996
Size: 2392 1604
NarI GG'CG_CC
Cuts at: 0 1215 3996
Size: 1215 2781
NcoI C'CATG_G
Cuts at: 0 996 2459 2594 2654 3996
Size: 996 1463 135 60 1342
Fragments arranged by size:
1463 1342 996 135 60
NdeI CA'TA_TG
Cuts at: 0 1773 3996
Size: 1773 2223
NgoMIV G'CCGG_C
Cuts at: 0 2390 3996
Size: 2390 1606
NspI r_CATG'y
Cuts at: 0 133 216 708 806 1299 1420 2701 3996
Size: 133 83 492 98 493 121 1281 1295
Fragments arranged by size:
1295 1281 493 492 133 121 98 83
PasI CC'CwG_GG
Cuts at: 0 1666 2347 2554 3996
Size: 1666 681 207 1442
Fragments arranged by size:
1666 1442 681 207
PciI A'CATG_T
Cuts at: 0 802 3996
Size: 802 3194
PflMI CCAn_nnn'nTGG
Cuts at: 0 770 3186 3567 3798 3996
Size: 770 2416 381 231 198
Fragments arranged by size:
2416 770 381 231 198
PfoI T'CCnGG_A
Cuts at: 0 866 925 3996
Size: 866 59 3071
Fragments arranged by size:
3071 866 59
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2958 2990 3996
Size: 2958 32 1006
Fragments arranged by size:
2958 1006 32
PpuMI rG'GwC_Cy
Cuts at: 0 57 1678 1758 1939 2012 2057 2627 3996
Size: 57 1621 80 181 73 45 570 1369
Fragments arranged by size:
1621 1369 570 181 80 73 57 45
PshAI GACnn'nnGTC
Cuts at: 0 803 3996
Size: 803 3193
PspOMI G'GGCC_C
Cuts at: 0 1556 1669 2158 3008 3428 3996
Size: 1556 113 489 850 420 568
Fragments arranged by size:
1556 850 568 489 420 113
PstI C_TGCA'G
Cuts at: 0 403 1127 1265 1409 1439 1808 2957 3749
Size: 403 724 138 144 30 369 1149 792
Cuts at: 3749 3996
Size: 247
Fragments arranged by size:
1149 792 724 403 369 247 144 138
30
PvuII CAG'CTG
Cuts at: 0 147 531 1122 1365 1404 2952 3996
Size: 147 384 591 243 39 1548 1044
Fragments arranged by size:
1548 1044 591 384 243 147 39
SacI G_AGCT'C
Cuts at: 0 2848 3409 3879 3996
Size: 2848 561 470 117
Fragments arranged by size:
2848 561 470 117
SalI G'TCGA_C
Cuts at: 0 1044 3499 3574 3996
Size: 1044 2455 75 422
Fragments arranged by size:
2455 1044 422 75
SanDI GG'GwC_CC
Cuts at: 0 57 1758 2057 2627 3996
Size: 57 1701 299 570 1369
Fragments arranged by size:
1701 1369 570 299 57
SapI GCTCTTCn'nnn_
Cuts at: 0 268 3996
Size: 268 3728
SbfI CC_TGCA'GG
Cuts at: 0 1808 3996
Size: 1808 2188
ScaI AGT'ACT
Cuts at: 0 226 3996
Size: 226 3770
SexAI A'CCwGG_T
Cuts at: 0 344 3996
Size: 344 3652
SfcI C'TryA_G
Cuts at: 0 399 1123 1261 1405 1435 1804 2953 3745
Size: 399 724 138 144 30 369 1149 792
Cuts at: 3745 3996
Size: 251
Fragments arranged by size:
1149 792 724 399 369 251 144 138
30
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 2391 3996
Size: 2391 1605
SfoI GGC'GCC
Cuts at: 0 1216 3996
Size: 1216 2780
SmaI CCC'GGG
Cuts at: 0 284 3007 3996
Size: 284 2723 989
Fragments arranged by size:
2723 989 284
SmlI C'TyrA_G
Cuts at: 0 757 904 1426 3400 3529 3886 3996
Size: 757 147 522 1974 129 357 110
Fragments arranged by size:
1974 757 522 357 147 129 110
SphI G_CATG'C
Cuts at: 0 1420 2701 3996
Size: 1420 1281 1295
Fragments arranged by size:
1420 1295 1281
StuI AGG'CCT
Cuts at: 0 1660 3996
Size: 1660 2336
StyI C'CwwG_G
Cuts at: 0 996 1607 1997 2459 2594 2654 3996
Size: 996 611 390 462 135 60 1342
Fragments arranged by size:
1342 996 611 462 390 135 60
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 849 935 1890 2010 2769 2914 3146 3996
Size: 849 86 955 120 759 145 232 850
Fragments arranged by size:
955 850 849 759 232 145 120 86
TatI w'GTAC_w
Cuts at: 0 116 224 965 3038 3996
Size: 116 108 741 2073 958
Fragments arranged by size:
2073 958 741 116 108
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1742 1774 3996
Size: 1742 32 2222
Fragments arranged by size:
2222 1742 32
Tth111I GACn'n_nGTC
Cuts at: 0 2245 2584 2734 3856 3996
Size: 2245 339 150 1122 140
Fragments arranged by size:
2245 1122 339 150 140
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 162 1824 2457 2484 3795 3996
Size: 162 1662 633 27 1311 201
Fragments arranged by size:
1662 1311 633 201 162 27
XmaI C'CCGG_G
Cuts at: 0 282 3005 3996
Size: 282 2723 991
Fragments arranged by size:
2723 991 282
XmnI GAAnn'nnTTC
Cuts at: 0 559 3049 3996
Size: 559 2490 947
Fragments arranged by size:
2490 947 559
ZraI GAC'GTC
Cuts at: 0 2610 3996
Size: 2610 1386
Enzymes that do cut:
AarI AatII AccI AcuI AflII AflIII AhdI AleI
AlfI AlwNI ApaI ApaLI ApoI AvaI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BglI BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsiWI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI HaeII
Hin4I HincII KasI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NspI PasI PciI PflMI
PfoI PpiI PpuMI PshAI PspOMI PstI PvuII SacI
SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI
SfoI SmaI SmlI SphI StuI StyI TaqII TatI
TstI Tth111I XcmI XmaI XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AgeI AloI AscI AseI AsiSI
AvrII BaeI BamHI BclI BfrBI BglII BmtI BsaBI
BsiEI BssHII BstBI BstEII BstZ17I ClaI CspCI EagI
EcoRV FspI FspAI HindIII HpaI KpnI MfeI MluI
NheI NotI NruI NsiI PacI PmeI PmlI PsiI
PspXI PsrI PvuI RsrII SacII SgfI SgrAI SnaBI
SpeI SrfI SspI SwaI XbaI XhoI