Restriction map for
pFN21AE3606
(Linear) (Six-base) MAPSORT of: KIEE3606.seq Check: 6228 from: 1 to: 1437
KIEE3606.orf
With 182 enzymes: SgfI *
December 5, 2014 12:56 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 78 1437
Size: 78 1359
AccI GT'mk_AC
Cuts at: 0 641 1437
Size: 641 796
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1078 1437
Size: 1078 359
AfeI AGC'GCT
Cuts at: 0 367 1437
Size: 367 1070
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 310 342 1437
Size: 310 32 1095
Fragments arranged by size:
1095 310 32
AlwNI CAG_nnn'CTG
Cuts at: 0 180 249 516 1437
Size: 180 69 267 921
Fragments arranged by size:
921 267 180 69
ApaI G_GGCC'C
Cuts at: 0 39 136 195 1437
Size: 39 97 59 1242
Fragments arranged by size:
1242 97 59 39
AvaI C'yCGr_G
Cuts at: 0 28 69 94 129 194 379 980 1437
Size: 28 41 25 35 65 185 601 457
Fragments arranged by size:
601 457 185 65 41 35 28 25
BamHI G'GATC_C
Cuts at: 0 318 1437
Size: 318 1119
BanI G'GyrC_C
Cuts at: 0 90 1437
Size: 90 1347
BanII G_rGCy'C
Cuts at: 0 16 39 70 136 151 195 400 473
Size: 16 23 31 66 15 44 205 73
Cuts at: 473 1437
Size: 964
Fragments arranged by size:
964 205 73 66 44 31 23 16
15
BbsI GAAGACnn'nnnn_
Cuts at: 0 441 1370 1437
Size: 441 929 67
Fragments arranged by size:
929 441 67
BglI GCCn_nnn'nGGC
Cuts at: 0 259 268 286 355 477 1437
Size: 259 9 18 69 122 960
Fragments arranged by size:
960 259 122 69 18 9
BglII A'GATC_T
Cuts at: 0 1143 1277 1437
Size: 1143 134 160
Fragments arranged by size:
1143 160 134
Bme1580I G_kGCm'C
Cuts at: 0 39 95 136 195 1437
Size: 39 56 41 59 1242
Fragments arranged by size:
1242 59 56 41 39
BmrI ACTGGGnnnn_n'
Cuts at: 0 1002 1104 1437
Size: 1002 102 333
Fragments arranged by size:
1002 333 102
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 384 859 1095 1437
Size: 384 475 236 342
Fragments arranged by size:
475 384 342 236
Bpu10I CC'TnA_GC
Cuts at: 0 257 284 1437
Size: 257 27 1153
Fragments arranged by size:
1153 257 27
BsaI GGTCTCn'nnnn_
Cuts at: 0 649 1437
Size: 649 788
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 382 412 857 887 1437
Size: 382 30 445 30 550
Fragments arranged by size:
550 445 382 30 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1306 1437
Size: 1306 131
BsiEI CG_ry'CG
Cuts at: 0 125 165 1437
Size: 125 40 1272
Fragments arranged by size:
1272 125 40
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1 1348 1437
Size: 1 1347 89
Fragments arranged by size:
1347 89 1
Bsp1286I G_dGCh'C
Cuts at: 0 16 39 70 95 136 151 195 400
Size: 16 23 31 25 41 15 44 205
Cuts at: 400 473 1437
Size: 73 964
Fragments arranged by size:
964 205 73 44 41 31 25 23
16 15
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 78 1437
Size: 78 1359
BsrBI CCG'CTC
Cuts at: 0 33 171 256 283 300 885 1437
Size: 33 138 85 27 17 585 552
Fragments arranged by size:
585 552 138 85 33 27 17
BsrDI GCAATG_nn'
Cuts at: 0 440 936 1437
Size: 440 496 501
Fragments arranged by size:
501 496 440
BsrFI r'CCGG_y
Cuts at: 0 124 860 1437
Size: 124 736 577
Fragments arranged by size:
736 577 124
BstAPI GCAn_nnn'nTGC
Cuts at: 0 870 1437
Size: 870 567
BstXI CCAn_nnnn'nTGG
Cuts at: 0 594 1437
Size: 594 843
BstYI r'GATC_y
Cuts at: 0 318 1143 1221 1277 1437
Size: 318 825 78 56 160
Fragments arranged by size:
825 318 160 78 56
BstZ17I GTA'TAC
Cuts at: 0 642 1437
Size: 642 795
Bsu36I CC'TnA_GG
Cuts at: 0 428 1437
Size: 428 1009
BtgI C'CryG_G
Cuts at: 0 227 263 1437
Size: 227 36 1174
Fragments arranged by size:
1174 227 36
DrdI GACnn_nn'nnGTC
Cuts at: 0 550 1437
Size: 550 887
EaeI y'GGCC_r
Cuts at: 0 122 251 356 537 647 1437
Size: 122 129 105 181 110 790
Fragments arranged by size:
790 181 129 122 110 105
EagI C'GGCC_G
Cuts at: 0 122 1437
Size: 122 1315
EarI CTCTTCn'nnn_
Cuts at: 0 461 848 1187 1216 1437
Size: 461 387 339 29 221
Fragments arranged by size:
461 387 339 221 29
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 131 1437
Size: 131 1306
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 384 859 1078 1095 1437
Size: 384 475 219 17 342
Fragments arranged by size:
475 384 342 219 17
EcoNI CCTnn'n_nnAGG
Cuts at: 0 559 1437
Size: 559 878
EcoO109I rG'GnC_Cy
Cuts at: 0 133 423 1197 1437
Size: 133 290 774 240
Fragments arranged by size:
774 290 240 133
EcoRV GAT'ATC
Cuts at: 0 1163 1437
Size: 1163 274
HaeII r_GCGC'y
Cuts at: 0 77 369 1437
Size: 77 292 1068
Fragments arranged by size:
1068 292 77
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1068 1100 1128 1160 1269 1301 1437
Size: 1068 32 28 32 109 32 136
Fragments arranged by size:
1068 136 109 32 32 32 28
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 164 711 1437
Size: 164 547 726
Fragments arranged by size:
726 547 164
MscI TGG'CCA
Cuts at: 0 358 649 1437
Size: 358 291 788
Fragments arranged by size:
788 358 291
MspA1I CmG'CkG
Cuts at: 0 265 404 738 1374 1437
Size: 265 139 334 636 63
Fragments arranged by size:
636 334 265 139 63
NaeI GCC'GGC
Cuts at: 0 126 1437
Size: 126 1311
NgoMIV G'CCGG_C
Cuts at: 0 124 1437
Size: 124 1313
PasI CC'CwG_GG
Cuts at: 0 210 1437
Size: 210 1227
PflMI CCAn_nnn'nTGG
Cuts at: 0 228 249 606 730 769 1437
Size: 228 21 357 124 39 668
Fragments arranged by size:
668 357 228 124 39 21
PfoI T'CCnGG_A
Cuts at: 0 311 1072 1437
Size: 311 761 365
Fragments arranged by size:
761 365 311
PpuMI rG'GwC_Cy
Cuts at: 0 423 1197 1437
Size: 423 774 240
Fragments arranged by size:
774 423 240
PspOMI G'GGCC_C
Cuts at: 0 35 132 191 1437
Size: 35 97 59 1246
Fragments arranged by size:
1246 97 59 35
PstI C_TGCA'G
Cuts at: 0 512 1437
Size: 512 925
RsrII CG'GwC_CG
Cuts at: 0 105 1437
Size: 105 1332
SacII CC_GC'GG
Cuts at: 0 266 1437
Size: 266 1171
SapI GCTCTTCn'nnn_
Cuts at: 0 461 1437
Size: 461 976
SfcI C'TryA_G
Cuts at: 0 508 666 1437
Size: 508 158 771
Fragments arranged by size:
771 508 158
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 259 355 1437
Size: 259 96 1082
Fragments arranged by size:
1082 259 96
SmaI CCC'GGG
Cuts at: 0 96 196 1437
Size: 96 100 1241
Fragments arranged by size:
1241 100 96
SpeI A'CTAG_T
Cuts at: 0 18 1437
Size: 18 1419
StuI AGG'CCT
Cuts at: 0 1297 1437
Size: 1297 140
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 695 984 1437
Size: 695 289 453
Fragments arranged by size:
695 453 289
XmaI C'CCGG_G
Cuts at: 0 94 194 1437
Size: 94 100 1243
Fragments arranged by size:
1243 100 94
XmnI GAAnn'nnTTC
Cuts at: 0 307 842 1420 1437
Size: 307 535 578 17
Fragments arranged by size:
578 535 307 17
Enzymes that do cut:
AarI AccI AcuI AfeI AloI AlwNI ApaI AvaI
BamHI BanI BanII BbsI BglI BglII Bme1580I BmrI
BpmI Bpu10I BsaI BsaXI BsgI BsiEI BsmBI Bsp1286I
BspMI BsrBI BsrDI BsrFI BstAPI BstXI BstYI BstZ17I
Bsu36I BtgI DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRV HaeII Hin4I MmeI MscI MspA1I
NaeI NgoMIV PasI PflMI PfoI PpuMI PspOMI PstI
RsrII SacII SapI SfcI SfiI SmaI SpeI StuI
TaqII XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AflII AflIII AgeI AhdI AleI
AlfI ApaLI ApoI AscI AseI AsiSI AvrII BaeI
BbeI BbvCI BcgI BciVI BclI BfrBI BlpI BmgBI
BmtI BplI BpuEI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsiHKAI BsiWI BsmI BspEI BspHI BsrGI BssHII
BssSI BstBI BstEII BtgZI BtsI ClaI CspCI DraI
DraIII EcoICRI EcoRI FalI FseI FspI FspAI HincII
HindIII HpaI KasI KpnI MfeI MluI MslI NarI
NcoI NdeI NheI NotI NruI NsiI NspI PacI
PciI PmeI PmlI PpiI PshAI PsiI PspXI PsrI
PvuI PvuII SacI SalI SanDI SbfI ScaI SexAI
SfoI SgfI SgrAI SmlI SnaBI SphI SrfI SspI
StyI SwaI TatI TstI Tth111I XbaI XcmI XhoI
ZraI