Restriction map for
pFN21AE3603
(Linear) (Six-base) MAPSORT of: KIEE3603.seq Check: 9388 from: 1 to: 633
KIEE3603.orf
With 182 enzymes: SgfI *
December 27, 2011 16:35 ..
AatII G_ACGT'C
Cuts at: 0 575 633
Size: 575 58
AfeI AGC'GCT
Cuts at: 0 82 633
Size: 82 551
AgeI A'CCGG_T
Cuts at: 0 354 633
Size: 354 279
ApaLI G'TGCA_C
Cuts at: 0 88 633
Size: 88 545
BbsI GAAGACnn'nnnn_
Cuts at: 0 70 258 633
Size: 70 188 375
Fragments arranged by size:
375 188 70
Bme1580I G_kGCm'C
Cuts at: 0 92 633
Size: 92 541
BmrI ACTGGGnnnn_n'
Cuts at: 0 165 633
Size: 165 468
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 492 633
Size: 492 141
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 127 406 633
Size: 127 279 227
Fragments arranged by size:
279 227 127
BsaAI yAC'GTr
Cuts at: 0 87 633
Size: 87 546
BsaHI Gr'CG_yC
Cuts at: 0 572 633
Size: 572 61
BsaWI w'CCGG_w
Cuts at: 0 354 633
Size: 354 279
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 10 40 633
Size: 10 30 593
Fragments arranged by size:
593 30 10
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 93 633
Size: 93 540
BsiHKAI G_wGCw'C
Cuts at: 0 23 92 152 407 449 633
Size: 23 69 60 255 42 184
Fragments arranged by size:
255 184 69 60 42 23
Bsp1286I G_dGCh'C
Cuts at: 0 23 92 152 407 449 633
Size: 23 69 60 255 42 184
Fragments arranged by size:
255 184 69 60 42 23
BspHI T'CATG_A
Cuts at: 0 407 633
Size: 407 226
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 190 633
Size: 190 443
BsrFI r'CCGG_y
Cuts at: 0 354 633
Size: 354 279
BsrGI T'GTAC_A
Cuts at: 0 26 633
Size: 26 607
BstXI CCAn_nnnn'nTGG
Cuts at: 0 516 633
Size: 516 117
BtsI GCAGTG_nn'
Cuts at: 0 305 633
Size: 305 328
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 110 145 633
Size: 110 35 488
Fragments arranged by size:
488 110 35
EaeI y'GGCC_r
Cuts at: 0 374 633
Size: 374 259
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 492 633
Size: 492 141
EcoO109I rG'GnC_Cy
Cuts at: 0 6 159 633
Size: 6 153 474
Fragments arranged by size:
474 153 6
EcoRV GAT'ATC
Cuts at: 0 195 633
Size: 195 438
HaeII r_GCGC'y
Cuts at: 0 84 633
Size: 84 549
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 310 342 490 522 633
Size: 310 32 148 32 111
Fragments arranged by size:
310 148 111 32 32
MscI TGG'CCA
Cuts at: 0 376 633
Size: 376 257
MspA1I CmG'CkG
Cuts at: 0 611 633
Size: 611 22
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 88 120 633
Size: 88 32 513
Fragments arranged by size:
513 88 32
PpuMI rG'GwC_Cy
Cuts at: 0 159 633
Size: 159 474
PshAI GACnn'nnGTC
Cuts at: 0 229 633
Size: 229 404
PstI C_TGCA'G
Cuts at: 0 185 633
Size: 185 448
PvuII CAG'CTG
Cuts at: 0 611 633
Size: 611 22
SanDI GG'GwC_CC
Cuts at: 0 159 633
Size: 159 474
SfcI C'TryA_G
Cuts at: 0 181 633
Size: 181 452
SmlI C'TyrA_G
Cuts at: 0 142 421 633
Size: 142 279 212
Fragments arranged by size:
279 212 142
StyI C'CwwG_G
Cuts at: 0 599 633
Size: 599 34
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 241 633
Size: 241 392
TatI w'GTAC_w
Cuts at: 0 26 633
Size: 26 607
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 126 633
Size: 126 507
ZraI GAC'GTC
Cuts at: 0 573 633
Size: 573 60
Enzymes that do cut:
AatII AfeI AgeI ApaLI BbsI Bme1580I BmrI BpmI
BpuEI BsaAI BsaHI BsaWI BsaXI BseRI BsiHKAI Bsp1286I
BspHI BspMI BsrFI BsrGI BstXI BtsI CspCI EaeI
Eco57MI EcoO109I EcoRV HaeII Hin4I MscI MspA1I PpiI
PpuMI PshAI PstI PvuII SanDI SfcI SmlI StyI
TaqII TatI XcmI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AhdI
AleI AlfI AloI AlwNI ApaI ApoI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
BmgBI BmtI BplI Bpu10I BsaI BsaBI BseYI BsgI
BsiEI BsiWI BsmI BsmBI BspEI BsrBI BsrDI BssHII
BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI
BtgZI ClaI DraI DraIII DrdI EagI EarI EciI
EcoICRI EcoNI EcoRI FalI FseI FspI FspAI HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MslI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PasI PciI PflMI PfoI PmeI
PmlI PsiI PspOMI PspXI PsrI PvuI RsrII SacI
SacII SalI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
StuI SwaI TstI Tth111I XbaI XhoI XmaI XmnI