Restriction map for
pFN21AE3601
(Linear) (Six-base) MAPSORT of: KIEE3601.seq Check: 757 from: 1 to: 537
KIEE3601.orf
With 182 enzymes: SgfI *
December 27, 2011 16:35 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 373 537
Size: 373 164
AlwNI CAG_nnn'CTG
Cuts at: 0 114 537
Size: 114 423
AvaI C'yCGr_G
Cuts at: 0 80 537
Size: 80 457
BanI G'GyrC_C
Cuts at: 0 73 537
Size: 73 464
BanII G_rGCy'C
Cuts at: 0 464 536 537
Size: 464 72 1
Fragments arranged by size:
464 72 1
BbeI G_GCGC'C
Cuts at: 0 77 537
Size: 77 460
BbsI GAAGACnn'nnnn_
Cuts at: 0 230 537
Size: 230 307
BlpI GC'TnA_GC
Cuts at: 0 59 537
Size: 59 478
BmrI ACTGGGnnnn_n'
Cuts at: 0 251 537
Size: 251 286
BsaHI Gr'CG_yC
Cuts at: 0 74 537
Size: 74 463
BsaWI w'CCGG_w
Cuts at: 0 87 537
Size: 87 450
BseYI C'CCAG_C
Cuts at: 0 21 537
Size: 21 516
BsiHKAI G_wGCw'C
Cuts at: 0 464 537
Size: 464 73
Bsp1286I G_dGCh'C
Cuts at: 0 464 536 537
Size: 464 72 1
Fragments arranged by size:
464 72 1
BspHI T'CATG_A
Cuts at: 0 125 537
Size: 125 412
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 503 507 537
Size: 503 4 30
Fragments arranged by size:
503 30 4
BsrBI CCG'CTC
Cuts at: 0 68 537
Size: 68 469
BstEII G'GTnAC_C
Cuts at: 0 519 537
Size: 519 18
Bsu36I CC'TnA_GG
Cuts at: 0 229 537
Size: 229 308
BtgI C'CryG_G
Cuts at: 0 198 537
Size: 198 339
EcoICRI GAG'CTC
Cuts at: 0 462 537
Size: 462 75
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 373 537
Size: 373 164
HaeII r_GCGC'y
Cuts at: 0 77 537
Size: 77 460
KasI G'GCGC_C
Cuts at: 0 73 537
Size: 73 464
MspA1I CmG'CkG
Cuts at: 0 208 537
Size: 208 329
NarI GG'CG_CC
Cuts at: 0 74 537
Size: 74 463
NcoI C'CATG_G
Cuts at: 0 198 537
Size: 198 339
NdeI CA'TA_TG
Cuts at: 0 337 537
Size: 337 200
NspI r_CATG'y
Cuts at: 0 454 537
Size: 454 83
PasI CC'CwG_GG
Cuts at: 0 327 537
Size: 327 210
PvuII CAG'CTG
Cuts at: 0 208 537
Size: 208 329
SacI G_AGCT'C
Cuts at: 0 464 537
Size: 464 73
SfcI C'TryA_G
Cuts at: 0 103 537
Size: 103 434
SfoI GGC'GCC
Cuts at: 0 75 537
Size: 75 462
SmaI CCC'GGG
Cuts at: 0 82 537
Size: 82 455
StyI C'CwwG_G
Cuts at: 0 198 537
Size: 198 339
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 340 537
Size: 340 197
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 205 537
Size: 205 332
XmaI C'CCGG_G
Cuts at: 0 80 537
Size: 80 457
Enzymes that do cut:
AcuI AlwNI AvaI BanI BanII BbeI BbsI BlpI
BmrI BsaHI BsaWI BseYI BsiHKAI Bsp1286I BspHI BspMI
BsrBI BstEII Bsu36I BtgI EcoICRI Eco57MI HaeII KasI
MspA1I NarI NcoI NdeI NspI PasI PvuII SacI
SfcI SfoI SmaI StyI TaqII XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaI ApaLI ApoI
AscI AseI AsiSI AvrII BaeI BamHI BbvCI BcgI
BciVI BclI BfrBI BglI BglII Bme1580I BmgBI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaXI
BseRI BsgI BsiEI BsiWI BsmI BsmBI BspEI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstXI BstYI
BstZ17I BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoNI EcoO109I EcoRI EcoRV
FalI FseI FspI FspAI Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI NaeI NgoMIV
NheI NotI NruI NsiI PacI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI RsrII SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SgfI SgrAI SmlI SnaBI
SpeI SphI SrfI SspI StuI SwaI TatI TstI
Tth111I XbaI XhoI XmnI ZraI