Restriction map for
pFN21AE3596
(Linear) (Six-base) MAPSORT of: KIEE3596.seq Check: 3359 from: 1 to: 2349
KIEE3596.orf
With 182 enzymes: SgfI *
March 22, 2012 16:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2201 2349
Size: 2201 148
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1768 2349
Size: 1768 581
AgeI A'CCGG_T
Cuts at: 0 824 2349
Size: 824 1525
AleI CACnn'nnGTG
Cuts at: 0 362 1648 2349
Size: 362 1286 701
Fragments arranged by size:
1286 701 362
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2031 2349
Size: 2031 318
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1667 1699 2349
Size: 1667 32 650
Fragments arranged by size:
1667 650 32
AlwNI CAG_nnn'CTG
Cuts at: 0 924 942 1584 1767 1977 2181 2349
Size: 924 18 642 183 210 204 168
Fragments arranged by size:
924 642 210 204 183 168 18
ApoI r'AATT_y
Cuts at: 0 310 513 2349
Size: 310 203 1836
Fragments arranged by size:
1836 310 203
AvaI C'yCGr_G
Cuts at: 0 1066 2349
Size: 1066 1283
AvrII C'CTAG_G
Cuts at: 0 2325 2349
Size: 2325 24
BanI G'GyrC_C
Cuts at: 0 356 1119 1564 1591 2203 2349
Size: 356 763 445 27 612 146
Fragments arranged by size:
763 612 445 356 146 27
BanII G_rGCy'C
Cuts at: 0 559 845 1099 1226 1234 1559 1879 1903
Size: 559 286 254 127 8 325 320 24
Cuts at: 1903 2117 2273 2349
Size: 214 156 76
Fragments arranged by size:
559 325 320 286 254 214 156 127
76 24 8
BbeI G_GCGC'C
Cuts at: 0 1595 2349
Size: 1595 754
BbsI GAAGACnn'nnnn_
Cuts at: 0 1780 2349
Size: 1780 569
BbvCI CC'TCA_GC
Cuts at: 0 1768 2166 2349
Size: 1768 398 183
Fragments arranged by size:
1768 398 183
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 786 820 2349
Size: 786 34 1529
Fragments arranged by size:
1529 786 34
BglI GCCn_nnn'nGGC
Cuts at: 0 1077 1855 1913 1937 2170 2254 2277 2349
Size: 1077 778 58 24 233 84 23 72
Fragments arranged by size:
1077 778 233 84 72 58 24 23
BlpI GC'TnA_GC
Cuts at: 0 839 2349
Size: 839 1510
Bme1580I G_kGCm'C
Cuts at: 0 359 1171 2234 2349
Size: 359 812 1063 115
Fragments arranged by size:
1063 812 359 115
BmgBI CAC'GTC
Cuts at: 0 2108 2349
Size: 2108 241
BmrI ACTGGGnnnn_n'
Cuts at: 0 1047 1642 2349
Size: 1047 595 707
Fragments arranged by size:
1047 707 595
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 642 730 1186 1210 1962 1966 1972 2349
Size: 642 88 456 24 752 4 6 377
Fragments arranged by size:
752 642 456 377 88 24 6 4
Bpu10I CC'TnA_GC
Cuts at: 0 1661 1768 1873 2166 2349
Size: 1661 107 105 293 183
Fragments arranged by size:
1661 293 183 107 105
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 433 728 1018 1045 1724 2349
Size: 433 295 290 27 679 625
Fragments arranged by size:
679 625 433 295 290 27
BsaI GGTCTCn'nnnn_
Cuts at: 0 1434 2349
Size: 1434 915
BsaAI yAC'GTr
Cuts at: 0 545 2349
Size: 545 1804
BsaHI Gr'CG_yC
Cuts at: 0 1592 1720 2349
Size: 1592 128 629
Fragments arranged by size:
1592 629 128
BsaWI w'CCGG_w
Cuts at: 0 824 2349
Size: 824 1525
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1667 1697 2349
Size: 1667 30 652
Fragments arranged by size:
1667 652 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1009 1351 1378 1381 1691 1969 2092 2349
Size: 1009 342 27 3 310 278 123 257
Fragments arranged by size:
1009 342 310 278 257 123 27 3
BseYI C'CCAG_C
Cuts at: 0 608 924 1393 1587 2030 2092 2349
Size: 608 316 469 194 443 62 257
Fragments arranged by size:
608 469 443 316 257 194 62
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1210 1877 1894 1991 2179 2349
Size: 1210 667 17 97 188 170
Fragments arranged by size:
1210 667 188 170 97 17
BsiEI CG_ry'CG
Cuts at: 0 407 2003 2349
Size: 407 1596 346
Fragments arranged by size:
1596 407 346
BsiHKAI G_wGCw'C
Cuts at: 0 1099 1226 1234 2349
Size: 1099 127 8 1115
Fragments arranged by size:
1115 1099 127 8
BsmI GAATG_Cn'
Cuts at: 0 1053 2349
Size: 1053 1296
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1476 2349
Size: 1476 873
Bsp1286I G_dGCh'C
Cuts at: 0 359 559 845 1099 1171 1226 1234 1559
Size: 359 200 286 254 72 55 8 325
Cuts at: 1559 1879 1903 2117 2234 2273 2349
Size: 320 24 214 117 39 76
Fragments arranged by size:
359 325 320 286 254 214 200 117
76 72 55 39 24 8
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 144 692 1164 2201 2349
Size: 144 548 472 1037 148
Fragments arranged by size:
1037 548 472 148 144
BsrBI CCG'CTC
Cuts at: 0 90 1029 2088 2349
Size: 90 939 1059 261
Fragments arranged by size:
1059 939 261 90
BsrFI r'CCGG_y
Cuts at: 0 824 953 1924 2034 2146 2206 2349
Size: 824 129 971 110 112 60 143
Fragments arranged by size:
971 824 143 129 112 110 60
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1495 1977 2349
Size: 1495 482 372
Fragments arranged by size:
1495 482 372
BstYI r'GATC_y
Cuts at: 0 812 2349
Size: 812 1537
Bsu36I CC'TnA_GG
Cuts at: 0 1124 2349
Size: 1124 1225
BtgI C'CryG_G
Cuts at: 0 32 407 1084 1484 1550 2349
Size: 32 375 677 400 66 799
Fragments arranged by size:
799 677 400 375 66 32
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 789 2349
Size: 789 1560
BtsI GCAGTG_nn'
Cuts at: 0 973 1246 2349
Size: 973 273 1103
Fragments arranged by size:
1103 973 273
DraIII CAC_nnn'GTG
Cuts at: 0 548 2349
Size: 548 1801
DrdI GACnn_nn'nnGTC
Cuts at: 0 969 2151 2349
Size: 969 1182 198
Fragments arranged by size:
1182 969 198
EaeI y'GGCC_r
Cuts at: 0 404 1421 1571 2000 2246 2349
Size: 404 1017 150 429 246 103
Fragments arranged by size:
1017 429 404 246 150 103
EagI C'GGCC_G
Cuts at: 0 404 2000 2349
Size: 404 1596 349
Fragments arranged by size:
1596 404 349
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 754 1629 2349
Size: 754 875 720
Fragments arranged by size:
875 754 720
EcoICRI GAG'CTC
Cuts at: 0 1097 1224 1232 2349
Size: 1097 127 8 1117
Fragments arranged by size:
1117 1097 127 8
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 642 730 1186 1210 1768 1962 1966 1972
Size: 642 88 456 24 558 194 4 6
Cuts at: 1972 2349
Size: 377
Fragments arranged by size:
642 558 456 377 194 88 24 6
4
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1127 1363 1423 1951 2349
Size: 1127 236 60 528 398
Fragments arranged by size:
1127 528 398 236 60
EcoO109I rG'GnC_Cy
Cuts at: 0 444 604 856 1842 2025 2307 2349
Size: 444 160 252 986 183 282 42
Fragments arranged by size:
986 444 282 252 183 160 42
HaeII r_GCGC'y
Cuts at: 0 925 1595 2349
Size: 925 670 754
Fragments arranged by size:
925 754 670
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 523 555 2349
Size: 523 32 1794
Fragments arranged by size:
1794 523 32
HindIII A'AGCT_T
Cuts at: 0 235 262 2349
Size: 235 27 2087
Fragments arranged by size:
2087 235 27
KasI G'GCGC_C
Cuts at: 0 1591 2349
Size: 1591 758
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 374 920 1176 1703 2349
Size: 374 546 256 527 646
Fragments arranged by size:
646 546 527 374 256
MscI TGG'CCA
Cuts at: 0 2248 2349
Size: 2248 101
MslI CAynn'nnrTG
Cuts at: 0 362 775 1648 2349
Size: 362 413 873 701
Fragments arranged by size:
873 701 413 362
MspA1I CmG'CkG
Cuts at: 0 34 228 612 702 1086 1271 1277 1552
Size: 34 194 384 90 384 185 6 275
Cuts at: 1552 1767 2005 2121 2170 2227 2349
Size: 215 238 116 49 57 122
Fragments arranged by size:
384 384 275 238 215 194 185 122
116 90 57 49 34 6
NaeI GCC'GGC
Cuts at: 0 1926 2036 2349
Size: 1926 110 313
Fragments arranged by size:
1926 313 110
NarI GG'CG_CC
Cuts at: 0 1592 2349
Size: 1592 757
NgoMIV G'CCGG_C
Cuts at: 0 1924 2034 2349
Size: 1924 110 315
Fragments arranged by size:
1924 315 110
NspI r_CATG'y
Cuts at: 0 294 774 2349
Size: 294 480 1575
Fragments arranged by size:
1575 480 294
PasI CC'CwG_GG
Cuts at: 0 96 942 2074 2274 2349
Size: 96 846 1132 200 75
Fragments arranged by size:
1132 846 200 96 75
PflMI CCAn_nnn'nTGG
Cuts at: 0 276 2349
Size: 276 2073
PfoI T'CCnGG_A
Cuts at: 0 1703 2180 2349
Size: 1703 477 169
Fragments arranged by size:
1703 477 169
PmlI CAC'GTG
Cuts at: 0 545 2349
Size: 545 1804
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1667 1699 2349
Size: 1667 32 650
Fragments arranged by size:
1667 650 32
PpuMI rG'GwC_Cy
Cuts at: 0 444 856 1842 2307 2349
Size: 444 412 986 465 42
Fragments arranged by size:
986 465 444 412 42
PshAI GACnn'nnGTC
Cuts at: 0 1520 2349
Size: 1520 829
PstI C_TGCA'G
Cuts at: 0 1241 1276 1500 1658 2144 2288 2349
Size: 1241 35 224 158 486 144 61
Fragments arranged by size:
1241 486 224 158 144 61 35
PvuII CAG'CTG
Cuts at: 0 228 612 1271 1767 2349
Size: 228 384 659 496 582
Fragments arranged by size:
659 582 496 384 228
SacI G_AGCT'C
Cuts at: 0 1099 1226 1234 2349
Size: 1099 127 8 1115
Fragments arranged by size:
1115 1099 127 8
SacII CC_GC'GG
Cuts at: 0 35 1087 1553 2349
Size: 35 1052 466 796
Fragments arranged by size:
1052 796 466 35
SanDI GG'GwC_CC
Cuts at: 0 856 2349
Size: 856 1493
ScaI AGT'ACT
Cuts at: 0 415 2349
Size: 415 1934
SexAI A'CCwGG_T
Cuts at: 0 269 2349
Size: 269 2080
SfcI C'TryA_G
Cuts at: 0 1237 1272 1496 1654 2140 2284 2349
Size: 1237 35 224 158 486 144 65
Fragments arranged by size:
1237 486 224 158 144 65 35
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 2254 2349
Size: 2254 95
SfoI GGC'GCC
Cuts at: 0 1593 2349
Size: 1593 756
SgrAI Cr'CCGG_yG
Cuts at: 0 824 2349
Size: 824 1525
SmaI CCC'GGG
Cuts at: 0 1068 2349
Size: 1068 1281
SmlI C'TyrA_G
Cuts at: 0 448 743 1024 1033 1739 2349
Size: 448 295 281 9 706 610
Fragments arranged by size:
706 610 448 295 281 9
SphI G_CATG'C
Cuts at: 0 774 2349
Size: 774 1575
StuI AGG'CCT
Cuts at: 0 1753 1907 2349
Size: 1753 154 442
Fragments arranged by size:
1753 442 154
StyI C'CwwG_G
Cuts at: 0 1184 2325 2349
Size: 1184 1141 24
Fragments arranged by size:
1184 1141 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 469 2349
Size: 469 1880
TatI w'GTAC_w
Cuts at: 0 413 2349
Size: 413 1936
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1476 1508 2349
Size: 1476 32 841
Fragments arranged by size:
1476 841 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1794 2349
Size: 1794 555
XmaI C'CCGG_G
Cuts at: 0 1066 2349
Size: 1066 1283
XmnI GAAnn'nnTTC
Cuts at: 0 385 2349
Size: 385 1964
Enzymes that do cut:
AarI AcuI AgeI AleI AlfI AloI AlwNI ApoI
AvaI AvrII BanI BanII BbeI BbsI BbvCI BcgI
BglI BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI
BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrBI BsrFI
BstAPI BstYI Bsu36I BtgI BtgZI BtsI DraIII DrdI
EaeI EagI EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII
Hin4I HindIII KasI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PasI PflMI PfoI PmlI PpiI
PpuMI PshAI PstI PvuII SacI SacII SanDI ScaI
SexAI SfcI SfiI SfoI SgrAI SmaI SmlI SphI
StuI StyI TaqII TatI TstI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AhdI
ApaI ApaLI AscI AseI AsiSI BaeI BamHI BciVI
BclI BfrBI BglII BmtI BplI BsaBI BsiWI BspEI
BspHI BsrDI BsrGI BssHII BssSI BstBI BstEII BstXI
BstZ17I ClaI CspCI DraI EarI EcoRI EcoRV FalI
FseI FspI FspAI HincII HpaI KpnI MfeI MluI
NcoI NdeI NheI NotI NruI NsiI PacI PciI
PmeI PsiI PspOMI PspXI PsrI PvuI RsrII SalI
SapI SbfI SgfI SnaBI SpeI SrfI SspI SwaI
Tth111I XbaI XhoI ZraI