(Linear) (Six-base) MAPSORT of: KIEE3595.seq  Check: 4609  from: 1  to: 1062



KIEE3595.orf

 With 182 enzymes: SgfI * 

                        December 27, 2011 16:35  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     664    1062 
   Size:        664     398
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      92    1062 
   Size:         92     970
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     342     374    1062 
   Size:        342      32     688

  Fragments arranged by size: 

                 688     342      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     493    1062 
   Size:        493     569
 
ApoI r'AATT_y

Cuts at:      0     582     769     997    1062 
   Size:        582     187     228      65

  Fragments arranged by size: 

                 582     228     187      65
 
BamHI G'GATC_C

Cuts at:      0     632    1062 
   Size:        632     430
 
BanII G_rGCy'C

Cuts at:      0     368    1062 
   Size:        368     694
 
BbvCI CC'TCA_GC

Cuts at:      0      20    1062 
   Size:         20    1042
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     409     443    1062 
   Size:        409      34     619

  Fragments arranged by size: 

                 619     409      34
 
BfrBI ATG'CAT

Cuts at:      0     729    1062 
   Size:        729     333
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     280    1062 
   Size:        280     782
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     220    1062 
   Size:        220     842
 
Bpu10I CC'TnA_GC

Cuts at:      0      20     362    1062 
   Size:         20     342     700

  Fragments arranged by size: 

                 700     342      20
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1027    1062 
   Size:       1027      35
 
BsaBI GATnn'nnATC

Cuts at:      0     160    1062 
   Size:        160     902
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     342     372    1062 
   Size:        342      30     690

  Fragments arranged by size: 

                 690     342      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     358    1062 
   Size:        358     704
 
BseYI C'CCAG_C

Cuts at:      0     114     121    1062 
   Size:        114       7     941

  Fragments arranged by size: 

                 941     114       7
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     692    1062 
   Size:        692     370
 
BsiHKAI G_wGCw'C

Cuts at:      0     142     368     524    1062 
   Size:        142     226     156     538

  Fragments arranged by size: 

                 538     226     156     142
 
BsmI GAATG_Cn'

Cuts at:      0     199    1062 
   Size:        199     863
 
Bsp1286I G_dGCh'C

Cuts at:      0     142     368     524    1062 
   Size:        142     226     156     538

  Fragments arranged by size: 

                 538     226     156     142
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     664    1062 
   Size:        664     398
 
BssSI C'ACGA_G

Cuts at:      0     367    1062 
   Size:        367     695
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     519    1062 
   Size:        519     543
 
BstYI r'GATC_y

Cuts at:      0     613     632    1062 
   Size:        613      19     430

  Fragments arranged by size: 

                 613     430      19
 
BtsI GCAGTG_nn'

Cuts at:      0     636    1062 
   Size:        636     426
 
ClaI AT'CG_AT

Cuts at:      0     278    1062 
   Size:        278     784
 
EaeI y'GGCC_r

Cuts at:      0      35    1062 
   Size:         35    1027
 
EcoICRI GAG'CTC

Cuts at:      0     366    1062 
   Size:        366     696
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     220    1062 
   Size:        220     842
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     820    1062 
   Size:        820     242
 
EcoO109I rG'GnC_Cy

Cuts at:      0     813    1062 
   Size:        813     249
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     834    1062 
   Size:        834     228
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     439     471     518     550    1062 
   Size:        439      32      47      32     512

  Fragments arranged by size: 

                 512     439      47      32      32
 
MscI TGG'CCA

Cuts at:      0      37    1062 
   Size:         37    1025
 
MslI CAynn'nnrTG

Cuts at:      0     173    1062 
   Size:        173     889
 
NdeI CA'TA_TG

Cuts at:      0     757    1062 
   Size:        757     305
 
NsiI A_TGCA'T

Cuts at:      0     731    1062 
   Size:        731     331
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     342     374    1062 
   Size:        342      32     688

  Fragments arranged by size: 

                 688     342      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     813    1062 
   Size:        813     249
 
PstI C_TGCA'G

Cuts at:      0      71     236     432    1062 
   Size:         71     165     196     630

  Fragments arranged by size: 

                 630     196     165      71
 
SacI G_AGCT'C

Cuts at:      0     368    1062 
   Size:        368     694
 
SfcI C'TryA_G

Cuts at:      0      67     232     428     957     977    1062 
   Size:         67     165     196     529      20      85

  Fragments arranged by size: 

                 529     196     165      85      67      20
 
SmlI C'TyrA_G

Cuts at:      0    1042    1062 
   Size:       1042      20
 
StyI C'CwwG_G

Cuts at:      0      47    1062 
   Size:         47    1015
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     106    1062 
   Size:        106     956
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     384     416    1062 
   Size:        384      32     646

  Fragments arranged by size: 

                 646     384      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     267    1062 
   Size:        267     795
 

 Enzymes that do cut:

     AarI     AlfI     AloI    AlwNI     ApoI    BamHI    BanII    BbvCI
     BcgI    BfrBI     BmrI     BpmI   Bpu10I    BpuEI    BsaBI    BsaXI
    BseRI    BseYI     BsgI  BsiHKAI     BsmI Bsp1286I    BspMI    BssSI
   BstAPI    BstYI     BtsI     ClaI     EaeI  EcoICRI  Eco57MI    EcoNI
 EcoO109I     FalI    Hin4I     MscI     MslI     NdeI     NsiI     PpiI
    PpuMI     PstI     SacI     SfcI     SmlI     StyI    TaqII     TstI
     XcmI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     ApaI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI     BanI     BbeI     BbsI    BciVI     BclI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmtI     BplI     BsaI
    BsaAI    BsaHI    BsaWI    BsiEI    BsiWI    BsmBI    BspEI    BspHI
    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BstBI   BstEII    BstXI
  BstZ17I   Bsu36I     BtgI    BtgZI    CspCI     DraI   DraIII     DrdI
     EagI     EarI     EciI    EcoRI    EcoRV     FseI     FspI    FspAI
    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NheI     NotI
     NruI     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII
    RsrII    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     SwaI     TatI  Tth111I     XbaI     XhoI
     XmaI     XmnI     ZraI