Restriction map for
pFN21AE3592
(Linear) (Six-base) MAPSORT of: KIEE3592.seq Check: 4708 from: 1 to: 744
KIEE3592.orf
With 182 enzymes: SgfI *
August 8, 2011 17:27 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 357 591 744
Size: 357 234 153
Fragments arranged by size:
357 234 153
AgeI A'CCGG_T
Cuts at: 0 212 744
Size: 212 532
AlwNI CAG_nnn'CTG
Cuts at: 0 709 744
Size: 709 35
ApaLI G'TGCA_C
Cuts at: 0 328 744
Size: 328 416
AvaI C'yCGr_G
Cuts at: 0 58 70 744
Size: 58 12 674
Fragments arranged by size:
674 58 12
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 298 331 744
Size: 298 33 413
Fragments arranged by size:
413 298 33
BanI G'GyrC_C
Cuts at: 0 3 744
Size: 3 741
BbsI GAAGACnn'nnnn_
Cuts at: 0 83 583 744
Size: 83 500 161
Fragments arranged by size:
500 161 83
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 445 479 744
Size: 445 34 265
Fragments arranged by size:
445 265 34
BglI GCCn_nnn'nGGC
Cuts at: 0 723 744
Size: 723 21
Bme1580I G_kGCm'C
Cuts at: 0 190 260 332 744
Size: 190 70 72 412
Fragments arranged by size:
412 190 72 70
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 584 632 744
Size: 584 48 112
Fragments arranged by size:
584 112 48
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 360 744
Size: 360 384
BsaWI w'CCGG_w
Cuts at: 0 212 744
Size: 212 532
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 186 216 476 506 744
Size: 186 30 260 30 238
Fragments arranged by size:
260 238 186 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 207 635 744
Size: 207 428 109
Fragments arranged by size:
428 207 109
BseYI C'CCAG_C
Cuts at: 0 668 744
Size: 668 76
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 145 187 744
Size: 145 42 557
Fragments arranged by size:
557 145 42
BsiHKAI G_wGCw'C
Cuts at: 0 332 744
Size: 332 412
Bsp1286I G_dGCh'C
Cuts at: 0 190 260 332 744
Size: 190 70 72 412
Fragments arranged by size:
412 190 72 70
BsrDI GCAATG_nn'
Cuts at: 0 308 744
Size: 308 436
BsrFI r'CCGG_y
Cuts at: 0 212 744
Size: 212 532
BsrGI T'GTAC_A
Cuts at: 0 392 744
Size: 392 352
BssSI C'ACGA_G
Cuts at: 0 189 744
Size: 189 555
Bsu36I CC'TnA_GG
Cuts at: 0 8 744
Size: 8 736
BtgI C'CryG_G
Cuts at: 0 356 488 744
Size: 356 132 256
Fragments arranged by size:
356 256 132
ClaI AT'CG_AT
Cuts at: 0 581 744
Size: 581 163
DraIII CAC_nnn'GTG
Cuts at: 0 183 744
Size: 183 561
EarI CTCTTCn'nnn_
Cuts at: 0 454 744
Size: 454 290
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 357 584 591 632 744
Size: 357 227 7 41 112
Fragments arranged by size:
357 227 112 41 7
EcoNI CCTnn'n_nnAGG
Cuts at: 0 647 744
Size: 647 97
EcoO109I rG'GnC_Cy
Cuts at: 0 664 744
Size: 664 80
EcoRV GAT'ATC
Cuts at: 0 365 744
Size: 365 379
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 186 218 476 508 744
Size: 186 32 258 32 236
Fragments arranged by size:
258 236 186 32 32
MslI CAynn'nnrTG
Cuts at: 0 355 744
Size: 355 389
NcoI C'CATG_G
Cuts at: 0 356 744
Size: 356 388
NdeI CA'TA_TG
Cuts at: 0 632 744
Size: 632 112
PflMI CCAn_nnn'nTGG
Cuts at: 0 709 744
Size: 709 35
PfoI T'CCnGG_A
Cuts at: 0 648 744
Size: 648 96
PpuMI rG'GwC_Cy
Cuts at: 0 664 744
Size: 664 80
SmaI CCC'GGG
Cuts at: 0 60 744
Size: 60 684
SmlI C'TyrA_G
Cuts at: 0 339 744
Size: 339 405
StyI C'CwwG_G
Cuts at: 0 356 744
Size: 356 388
TatI w'GTAC_w
Cuts at: 0 392 744
Size: 392 352
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 180 744
Size: 180 564
XmaI C'CCGG_G
Cuts at: 0 58 744
Size: 58 686
Enzymes that do cut:
AcuI AgeI AlwNI ApaLI AvaI BaeI BanI BbsI
BcgI BglI Bme1580I BpmI BpuEI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI Bsp1286I BsrDI BsrFI BsrGI BssSI
Bsu36I BtgI ClaI DraIII EarI Eco57MI EcoNI EcoO109I
EcoRV Hin4I MslI NcoI NdeI PflMI PfoI PpuMI
SmaI SmlI StyI TatI XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AhdI AleI AlfI AloI ApaI ApoI AscI AseI
AsiSI AvrII BamHI BanII BbeI BbvCI BciVI BclI
BfrBI BglII BlpI BmgBI BmrI BmtI BplI Bpu10I
BsaI BsaAI BsaBI BsaHI BsiEI BsiWI BsmI BsmBI
BspEI BspHI BspMI BsrBI BssHII BstAPI BstBI BstEII
BstXI BstYI BstZ17I BtgZI BtsI CspCI DraI DrdI
EaeI EagI EciI EcoICRI EcoRI FalI FseI FspI
FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MspA1I NaeI NarI NgoMIV NheI
NotI NruI NsiI NspI PacI PasI PciI PmeI
PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PstI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI
SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII
TstI Tth111I XbaI XhoI XmnI ZraI