Restriction map for
pFN21AE3591
(Linear) (Six-base) MAPSORT of: KIEE3591.seq Check: 788 from: 1 to: 1269
KIEE3591.orf
With 182 enzymes: SgfI *
December 27, 2011 16:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1241 1269
Size: 1241 28
AflIII A'CryG_T
Cuts at: 0 361 1269
Size: 361 908
AleI CACnn'nnGTG
Cuts at: 0 1066 1269
Size: 1066 203
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 321 1269
Size: 321 948
AlwNI CAG_nnn'CTG
Cuts at: 0 383 695 1010 1245 1256 1269
Size: 383 312 315 235 11 13
Fragments arranged by size:
383 315 312 235 13 11
ApaLI G'TGCA_C
Cuts at: 0 104 1269
Size: 104 1165
ApoI r'AATT_y
Cuts at: 0 788 1269
Size: 788 481
AvaI C'yCGr_G
Cuts at: 0 1111 1269
Size: 1111 158
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1024 1057 1269
Size: 1024 33 212
Fragments arranged by size:
1024 212 33
BamHI G'GATC_C
Cuts at: 0 986 1269
Size: 986 283
BanII G_rGCy'C
Cuts at: 0 1167 1269
Size: 1167 102
BbsI GAAGACnn'nnnn_
Cuts at: 0 295 1269
Size: 295 974
BclI T'GATC_A
Cuts at: 0 498 1269
Size: 498 771
BfrBI ATG'CAT
Cuts at: 0 887 1269
Size: 887 382
BglII A'GATC_T
Cuts at: 0 165 1269
Size: 165 1104
BlpI GC'TnA_GC
Cuts at: 0 247 1166 1269
Size: 247 919 103
Fragments arranged by size:
919 247 103
Bme1580I G_kGCm'C
Cuts at: 0 108 1269
Size: 108 1161
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 493 1057 1123 1269
Size: 493 564 66 146
Fragments arranged by size:
564 493 146 66
Bpu10I CC'TnA_GC
Cuts at: 0 373 949 997 1269
Size: 373 576 48 272
Fragments arranged by size:
576 373 272 48
BsaAI yAC'GTr
Cuts at: 0 362 658 1269
Size: 362 296 611
Fragments arranged by size:
611 362 296
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1045 1269
Size: 1045 224
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 558 700 976 1163 1269
Size: 558 142 276 187 106
Fragments arranged by size:
558 276 187 142 106
BsiHKAI G_wGCw'C
Cuts at: 0 108 1269
Size: 108 1161
BsmI GAATG_Cn'
Cuts at: 0 889 1269
Size: 889 380
Bsp1286I G_dGCh'C
Cuts at: 0 108 1167 1269
Size: 108 1059 102
Fragments arranged by size:
1059 108 102
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 817 1122 1241 1269
Size: 817 305 119 28
Fragments arranged by size:
817 305 119 28
BsrFI r'CCGG_y
Cuts at: 0 701 1269
Size: 701 568
BstEII G'GTnAC_C
Cuts at: 0 1134 1269
Size: 1134 135
BstYI r'GATC_y
Cuts at: 0 165 405 873 986 1269
Size: 165 240 468 113 283
Fragments arranged by size:
468 283 240 165 113
Bsu36I CC'TnA_GG
Cuts at: 0 238 565 1269
Size: 238 327 704
Fragments arranged by size:
704 327 238
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 237 1269
Size: 237 1032
BtsI GCAGTG_nn'
Cuts at: 0 1229 1269
Size: 1229 40
EaeI y'GGCC_r
Cuts at: 0 1014 1269
Size: 1014 255
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 493 1057 1123 1269
Size: 493 564 66 146
Fragments arranged by size:
564 493 146 66
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 267 1269
Size: 267 1002
HaeII r_GCGC'y
Cuts at: 0 1110 1269
Size: 1110 159
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 397 429 1149 1181 1269
Size: 397 32 720 32 88
Fragments arranged by size:
720 397 88 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1022 1269
Size: 1022 247
MscI TGG'CCA
Cuts at: 0 1016 1269
Size: 1016 253
MslI CAynn'nnrTG
Cuts at: 0 1066 1269
Size: 1066 203
NsiI A_TGCA'T
Cuts at: 0 889 1269
Size: 889 380
PasI CC'CwG_GG
Cuts at: 0 762 1269
Size: 762 507
PflMI CCAn_nnn'nTGG
Cuts at: 0 122 861 1269
Size: 122 739 408
Fragments arranged by size:
739 408 122
PmlI CAC'GTG
Cuts at: 0 362 1269
Size: 362 907
PspXI vC'TCGA_Gb
Cuts at: 0 1111 1269
Size: 1111 158
SfcI C'TryA_G
Cuts at: 0 706 1269
Size: 706 563
SmlI C'TyrA_G
Cuts at: 0 1111 1269
Size: 1111 158
SspI AAT'ATT
Cuts at: 0 68 1269
Size: 68 1201
StuI AGG'CCT
Cuts at: 0 26 548 1100 1269
Size: 26 522 552 169
Fragments arranged by size:
552 522 169 26
StyI C'CwwG_G
Cuts at: 0 116 1269
Size: 116 1153
XhoI C'TCGA_G
Cuts at: 0 1111 1269
Size: 1111 158
XmnI GAAnn'nnTTC
Cuts at: 0 69 1269
Size: 69 1200
Enzymes that do cut:
AarI AflIII AleI AlfI AlwNI ApaLI ApoI AvaI
BaeI BamHI BanII BbsI BclI BfrBI BglII BlpI
Bme1580I BpmI Bpu10I BsaAI BseRI BsgI BsiHKAI BsmI
Bsp1286I BspMI BsrFI BstEII BstYI Bsu36I BtgZI BtsI
EaeI Eco57MI FalI HaeII Hin4I MmeI MscI MslI
NsiI PasI PflMI PmlI PspXI SfcI SmlI SspI
StuI StyI XhoI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AgeI
AhdI AloI ApaI AscI AseI AsiSI AvrII BanI
BbeI BbvCI BcgI BciVI BglI BmgBI BmrI BmtI
BplI BpuEI BsaI BsaBI BsaHI BsaWI BsaXI BseYI
BsiEI BsiWI BsmBI BspEI BspHI BsrBI BsrDI BsrGI
BssHII BssSI BstAPI BstBI BstXI BstZ17I BtgI ClaI
CspCI DraI DraIII DrdI EagI EarI EciI EcoICRI
EcoNI EcoO109I EcoRI EcoRV FseI FspI FspAI HincII
HindIII HpaI KasI KpnI MfeI MluI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NspI
PacI PciI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PsrI PstI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SwaI
TaqII TatI TstI Tth111I XbaI XcmI XmaI ZraI