Restriction map for
pFN21AE3590
(Linear) (Six-base) MAPSORT of: KIEE3590.seq Check: 662 from: 1 to: 609
KIEE3590.orf
With 182 enzymes: SgfI *
December 27, 2011 16:35 ..
AatII G_ACGT'C
Cuts at: 0 23 416 609
Size: 23 393 193
Fragments arranged by size:
393 193 23
AccI GT'mk_AC
Cuts at: 0 393 609
Size: 393 216
AfeI AGC'GCT
Cuts at: 0 76 205 609
Size: 76 129 404
Fragments arranged by size:
404 129 76
AflIII A'CryG_T
Cuts at: 0 396 609
Size: 396 213
AlwNI CAG_nnn'CTG
Cuts at: 0 199 609
Size: 199 410
ApaLI G'TGCA_C
Cuts at: 0 82 609
Size: 82 527
BanI G'GyrC_C
Cuts at: 0 238 355 609
Size: 238 117 254
Fragments arranged by size:
254 238 117
BanII G_rGCy'C
Cuts at: 0 491 609
Size: 491 118
BbsI GAAGACnn'nnnn_
Cuts at: 0 386 609
Size: 386 223
BglI GCCn_nnn'nGGC
Cuts at: 0 136 609
Size: 136 473
BlpI GC'TnA_GC
Cuts at: 0 443 609
Size: 443 166
Bme1580I G_kGCm'C
Cuts at: 0 86 609
Size: 86 523
BmgBI CAC'GTC
Cuts at: 0 397 609
Size: 397 212
BsaI GGTCTCn'nnnn_
Cuts at: 0 152 609
Size: 152 457
BsaAI yAC'GTr
Cuts at: 0 51 81 609
Size: 51 30 528
Fragments arranged by size:
528 51 30
BsaHI Gr'CG_yC
Cuts at: 0 20 390 413 609
Size: 20 370 23 196
Fragments arranged by size:
370 196 23 20
BsaWI w'CCGG_w
Cuts at: 0 161 383 609
Size: 161 222 226
Fragments arranged by size:
226 222 161
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 343 609
Size: 343 266
BseYI C'CCAG_C
Cuts at: 0 36 609
Size: 36 573
BsiEI CG_ry'CG
Cuts at: 0 90 155 609
Size: 90 65 454
Fragments arranged by size:
454 90 65
BsiHKAI G_wGCw'C
Cuts at: 0 86 491 609
Size: 86 405 118
Fragments arranged by size:
405 118 86
Bsp1286I G_dGCh'C
Cuts at: 0 86 491 609
Size: 86 405 118
Fragments arranged by size:
405 118 86
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 377 609
Size: 377 232
BsrBI CCG'CTC
Cuts at: 0 5 609
Size: 5 604
BstYI r'GATC_y
Cuts at: 0 561 609
Size: 561 48
EaeI y'GGCC_r
Cuts at: 0 137 609
Size: 137 472
EarI CTCTTCn'nnn_
Cuts at: 0 496 609
Size: 496 113
EcoICRI GAG'CTC
Cuts at: 0 489 609
Size: 489 120
EcoNI CCTnn'n_nnAGG
Cuts at: 0 328 609
Size: 328 281
EcoO109I rG'GnC_Cy
Cuts at: 0 103 609
Size: 103 506
FspI TGC'GCA
Cuts at: 0 319 609
Size: 319 290
HaeII r_GCGC'y
Cuts at: 0 78 207 609
Size: 78 129 402
Fragments arranged by size:
402 129 78
HincII GTy'rAC
Cuts at: 0 394 609
Size: 394 215
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 135 609
Size: 135 474
MscI TGG'CCA
Cuts at: 0 139 609
Size: 139 470
MspA1I CmG'CkG
Cuts at: 0 596 609
Size: 596 13
PflMI CCAn_nnn'nTGG
Cuts at: 0 480 501 609
Size: 480 21 108
Fragments arranged by size:
480 108 21
PfoI T'CCnGG_A
Cuts at: 0 416 609
Size: 416 193
PvuII CAG'CTG
Cuts at: 0 596 609
Size: 596 13
SacI G_AGCT'C
Cuts at: 0 491 609
Size: 491 118
SalI G'TCGA_C
Cuts at: 0 392 609
Size: 392 217
SapI GCTCTTCn'nnn_
Cuts at: 0 496 609
Size: 496 113
SfcI C'TryA_G
Cuts at: 0 591 609
Size: 591 18
StyI C'CwwG_G
Cuts at: 0 140 242 609
Size: 140 102 367
Fragments arranged by size:
367 140 102
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 141 294 609
Size: 141 153 315
Fragments arranged by size:
315 153 141
ZraI GAC'GTC
Cuts at: 0 21 414 609
Size: 21 393 195
Fragments arranged by size:
393 195 21
Enzymes that do cut:
AatII AccI AfeI AflIII AlwNI ApaLI BanI BanII
BbsI BglI BlpI Bme1580I BmgBI BsaI BsaAI BsaHI
BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspMI BsrBI
BstYI EaeI EarI EcoICRI EcoNI EcoO109I FspI HaeII
HincII MmeI MscI MspA1I PflMI PfoI PvuII SacI
SalI SapI SfcI StyI TaqII ZraI
Enzymes that do not cut:
AarI Acc65I AclI AcuI AflII AgeI AhdI AleI
AlfI AloI ApaI ApoI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BbeI BbvCI BcgI BciVI BclI
BfrBI BglII BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaBI BsaXI BsgI BsiWI BsmI BsmBI BspEI BspHI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EagI EciI Eco57MI EcoRI EcoRV
FalI FseI FspAI Hin4I HindIII HpaI KasI KpnI
MfeI MluI MslI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI PacI PasI PciI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI RsrII SacII SanDI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI SwaI TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI