Restriction map for
pFN21AE3588
(Linear) (Six-base) MAPSORT of: KIEE3588.seq Check: 7188 from: 1 to: 2358
KIEE3588.orf
With 182 enzymes: SgfI *
December 27, 2011 16:35 ..
AatII G_ACGT'C
Cuts at: 0 2090 2358
Size: 2090 268
AccI GT'mk_AC
Cuts at: 0 1922 2358
Size: 1922 436
Acc65I G'GTAC_C
Cuts at: 0 1019 2358
Size: 1019 1339
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 205 283 387 406 2358
Size: 205 78 104 19 1952
Fragments arranged by size:
1952 205 104 78 19
AfeI AGC'GCT
Cuts at: 0 1561 2358
Size: 1561 797
AgeI A'CCGG_T
Cuts at: 0 1016 2358
Size: 1016 1342
AleI CACnn'nnGTG
Cuts at: 0 209 1228 2358
Size: 209 1019 1130
Fragments arranged by size:
1130 1019 209
AlwNI CAG_nnn'CTG
Cuts at: 0 240 709 1062 1287 2010 2358
Size: 240 469 353 225 723 348
Fragments arranged by size:
723 469 353 348 240 225
ApaI G_GGCC'C
Cuts at: 0 2160 2358
Size: 2160 198
ApoI r'AATT_y
Cuts at: 0 294 2358
Size: 294 2064
AvaI C'yCGr_G
Cuts at: 0 1695 2358
Size: 1695 663
BanI G'GyrC_C
Cuts at: 0 430 1019 1038 2358
Size: 430 589 19 1320
Fragments arranged by size:
1320 589 430 19
BanII G_rGCy'C
Cuts at: 0 1310 2160 2358
Size: 1310 850 198
Fragments arranged by size:
1310 850 198
BbsI GAAGACnn'nnnn_
Cuts at: 0 885 1454 1745 2358
Size: 885 569 291 613
Fragments arranged by size:
885 613 569 291
BbvCI CC'TCA_GC
Cuts at: 0 1657 1684 2358
Size: 1657 27 674
Fragments arranged by size:
1657 674 27
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1794 1828 2358
Size: 1794 34 530
Fragments arranged by size:
1794 530 34
BfrBI ATG'CAT
Cuts at: 0 2312 2358
Size: 2312 46
BglI GCCn_nnn'nGGC
Cuts at: 0 1354 2358
Size: 1354 1004
BlpI GC'TnA_GC
Cuts at: 0 413 1591 2358
Size: 413 1178 767
Fragments arranged by size:
1178 767 413
Bme1580I G_kGCm'C
Cuts at: 0 2160 2358
Size: 2160 198
BmgBI CAC'GTC
Cuts at: 0 1884 2358
Size: 1884 474
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1209 2358
Size: 1209 1149
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 236 841 1039 1717 1839 2358
Size: 236 605 198 678 122 519
Fragments arranged by size:
678 605 519 236 198 122
Bpu10I CC'TnA_GC
Cuts at: 0 1657 1684 2358
Size: 1657 27 674
Fragments arranged by size:
1657 674 27
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 117 1488 2358
Size: 117 1371 870
Fragments arranged by size:
1371 870 117
BsaHI Gr'CG_yC
Cuts at: 0 1499 2087 2358
Size: 1499 588 271
Fragments arranged by size:
1499 588 271
BsaWI w'CCGG_w
Cuts at: 0 1016 1432 2358
Size: 1016 416 926
Fragments arranged by size:
1016 926 416
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 87 613 646 661 1044 1804 1981 2097
Size: 87 526 33 15 383 760 177 116
Cuts at: 2097 2338 2341 2358
Size: 241 3 17
Fragments arranged by size:
760 526 383 241 177 116 87 33
17 15 3
BseYI C'CCAG_C
Cuts at: 0 179 315 764 1062 1280 1344 1800 1894
Size: 179 136 449 298 218 64 456 94
Cuts at: 1894 2282 2358
Size: 388 76
Fragments arranged by size:
456 449 388 298 218 179 136 94
76 64
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 922 976 1071 1303 1311 2358
Size: 922 54 95 232 8 1047
Fragments arranged by size:
1047 922 232 95 54 8
BsiHKAI G_wGCw'C
Cuts at: 0 119 1310 2358
Size: 119 1191 1048
Fragments arranged by size:
1191 1048 119
BsmBI CGTCTCn'nnnn_
Cuts at: 0 254 1197 1786 2358
Size: 254 943 589 572
Fragments arranged by size:
943 589 572 254
Bsp1286I G_dGCh'C
Cuts at: 0 119 1310 2160 2358
Size: 119 1191 850 198
Fragments arranged by size:
1191 850 198 119
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1031 2358
Size: 1031 1327
BsrBI CCG'CTC
Cuts at: 0 8 417 479 2358
Size: 8 409 62 1879
Fragments arranged by size:
1879 409 62 8
BsrDI GCAATG_nn'
Cuts at: 0 1941 2358
Size: 1941 417
BsrFI r'CCGG_y
Cuts at: 0 1016 2358
Size: 1016 1342
BsrGI T'GTAC_A
Cuts at: 0 171 2358
Size: 171 2187
BssSI C'ACGA_G
Cuts at: 0 505 2358
Size: 505 1853
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1073 2358
Size: 1073 1285
BstBI TT'CG_AA
Cuts at: 0 299 2358
Size: 299 2059
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1230 2055 2358
Size: 1230 825 303
Fragments arranged by size:
1230 825 303
BstYI r'GATC_y
Cuts at: 0 705 1109 1670 2027 2358
Size: 705 404 561 357 331
Fragments arranged by size:
705 561 404 357 331
Bsu36I CC'TnA_GG
Cuts at: 0 514 2097 2358
Size: 514 1583 261
Fragments arranged by size:
1583 514 261
BtgI C'CryG_G
Cuts at: 0 466 497 1001 1042 1916 2138 2358
Size: 466 31 504 41 874 222 220
Fragments arranged by size:
874 504 466 222 220 41 31
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 729 1053 2358
Size: 729 324 1305
Fragments arranged by size:
1305 729 324
BtsI GCAGTG_nn'
Cuts at: 0 561 1766 2280 2358
Size: 561 1205 514 78
Fragments arranged by size:
1205 561 514 78
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2120 2155 2358
Size: 2120 35 203
Fragments arranged by size:
2120 203 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 699 2358
Size: 699 1659
EaeI y'GGCC_r
Cuts at: 0 158 244 1376 2358
Size: 158 86 1132 982
Fragments arranged by size:
1132 982 158 86
EarI CTCTTCn'nnn_
Cuts at: 0 457 1577 2358
Size: 457 1120 781
Fragments arranged by size:
1120 781 457
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 448 1185 1323 1716 2358
Size: 448 737 138 393 642
Fragments arranged by size:
737 642 448 393 138
EcoICRI GAG'CTC
Cuts at: 0 1308 2358
Size: 1308 1050
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 205 236 283 387 406 841 1039 1717
Size: 205 31 47 104 19 435 198 678
Cuts at: 1717 1839 2358
Size: 122 519
Fragments arranged by size:
678 519 435 205 198 122 104 47
31 19
EcoNI CCTnn'n_nnAGG
Cuts at: 0 628 2358
Size: 628 1730
EcoO109I rG'GnC_Cy
Cuts at: 0 77 189 1164 1245 1425 1990 1998 2156
Size: 77 112 975 81 180 565 8 158
Cuts at: 2156 2157 2358
Size: 1 201
Fragments arranged by size:
975 565 201 180 158 112 81 77
8 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1503 2358
Size: 1503 855
HaeII r_GCGC'y
Cuts at: 0 1563 2017 2358
Size: 1563 454 341
Fragments arranged by size:
1563 454 341
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1819 1851 2182 2214 2358
Size: 1819 32 331 32 144
Fragments arranged by size:
1819 331 144 32 32
KpnI G_GTAC'C
Cuts at: 0 1023 2358
Size: 1023 1335
MscI TGG'CCA
Cuts at: 0 1378 2358
Size: 1378 980
MslI CAynn'nnrTG
Cuts at: 0 209 1228 2358
Size: 209 1019 1130
Fragments arranged by size:
1130 1019 209
MspA1I CmG'CkG
Cuts at: 0 183 531 777 1044 1059 1419 1545 1898
Size: 183 348 246 267 15 360 126 353
Cuts at: 1898 2358
Size: 460
Fragments arranged by size:
460 360 353 348 267 246 183 126
15
NsiI A_TGCA'T
Cuts at: 0 2314 2358
Size: 2314 44
NspI r_CATG'y
Cuts at: 0 224 2358
Size: 224 2134
PflMI CCAn_nnn'nTGG
Cuts at: 0 215 1062 2358
Size: 215 847 1296
Fragments arranged by size:
1296 847 215
PfoI T'CCnGG_A
Cuts at: 0 233 1121 1733 2358
Size: 233 888 612 625
Fragments arranged by size:
888 625 612 233
PpuMI rG'GwC_Cy
Cuts at: 0 189 1164 1245 1425 1990 1998 2358
Size: 189 975 81 180 565 8 360
Fragments arranged by size:
975 565 360 189 180 81 8
PshAI GACnn'nnGTC
Cuts at: 0 1258 2358
Size: 1258 1100
PspOMI G'GGCC_C
Cuts at: 0 2156 2358
Size: 2156 202
PstI C_TGCA'G
Cuts at: 0 536 2358
Size: 536 1822
PvuII CAG'CTG
Cuts at: 0 183 531 777 1059 1419 2358
Size: 183 348 246 282 360 939
Fragments arranged by size:
939 360 348 282 246 183
SacI G_AGCT'C
Cuts at: 0 1310 2358
Size: 1310 1048
SacII CC_GC'GG
Cuts at: 0 1045 2358
Size: 1045 1313
SanDI GG'GwC_CC
Cuts at: 0 1990 2358
Size: 1990 368
SapI GCTCTTCn'nnn_
Cuts at: 0 457 2358
Size: 457 1901
SexAI A'CCwGG_T
Cuts at: 0 191 1166 2358
Size: 191 975 1192
Fragments arranged by size:
1192 975 191
SfcI C'TryA_G
Cuts at: 0 24 143 532 1512 1596 2358
Size: 24 119 389 980 84 762
Fragments arranged by size:
980 762 389 119 84 24
SmlI C'TyrA_G
Cuts at: 0 96 1467 2358
Size: 96 1371 891
Fragments arranged by size:
1371 891 96
StuI AGG'CCT
Cuts at: 0 344 391 2358
Size: 344 47 1967
Fragments arranged by size:
1967 344 47
StyI C'CwwG_G
Cuts at: 0 1199 1505 2358
Size: 1199 306 853
Fragments arranged by size:
1199 853 306
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 418 2038 2358
Size: 418 1620 320
Fragments arranged by size:
1620 418 320
TatI w'GTAC_w
Cuts at: 0 171 2358
Size: 171 2187
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 535 567 809 841 2109 2141 2358
Size: 535 32 242 32 1268 32 217
Fragments arranged by size:
1268 535 242 217 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2136 2358
Size: 2136 222
XmnI GAAnn'nnTTC
Cuts at: 0 973 1486 2358
Size: 973 513 872
Fragments arranged by size:
973 872 513
ZraI GAC'GTC
Cuts at: 0 2088 2358
Size: 2088 270
Enzymes that do cut:
AatII AccI Acc65I AcuI AfeI AgeI AleI AlwNI
ApaI ApoI AvaI BanI BanII BbsI BbvCI BcgI
BfrBI BglI BlpI Bme1580I BmgBI BplI BpmI Bpu10I
BpuEI BsaHI BsaWI BseRI BseYI BsgI BsiHKAI BsmBI
Bsp1286I BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI
DrdI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
FalI HaeII Hin4I KpnI MscI MslI MspA1I NsiI
NspI PflMI PfoI PpuMI PshAI PspOMI PstI PvuII
SacI SacII SanDI SapI SexAI SfcI SmlI StuI
StyI TaqII TatI TstI XcmI XmnI ZraI
Enzymes that do not cut:
AarI AclI AflII AflIII AhdI AlfI AloI ApaLI
AscI AseI AsiSI AvrII BaeI BamHI BbeI BciVI
BclI BglII BmrI BmtI BsaI BsaAI BsaBI BsaXI
BsiEI BsiWI BsmI BspEI BspHI BssHII BstEII BstZ17I
ClaI DraI DraIII EagI EcoRI EcoRV FseI FspI
FspAI HincII HindIII HpaI KasI MfeI MluI MmeI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
PacI PasI PciI PmeI PmlI PpiI PsiI PspXI
PsrI PvuI RsrII SalI SbfI ScaI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
SwaI Tth111I XbaI XhoI XmaI