Restriction map for
pFN21AE3587
(Linear) (Six-base) MAPSORT of: KIEE3587.seq Check: 5801 from: 1 to: 714
KIEE3587.orf
With 182 enzymes: SgfI *
December 27, 2011 16:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 137 144 301 671 714
Size: 137 7 157 370 43
Fragments arranged by size:
370 157 137 43 7
AfeI AGC'GCT
Cuts at: 0 319 382 714
Size: 319 63 332
Fragments arranged by size:
332 319 63
AlwNI CAG_nnn'CTG
Cuts at: 0 129 714
Size: 129 585
ApaI G_GGCC'C
Cuts at: 0 74 217 236 562 714
Size: 74 143 19 326 152
Fragments arranged by size:
326 152 143 74 19
AvaI C'yCGr_G
Cuts at: 0 204 217 714
Size: 204 13 497
Fragments arranged by size:
497 204 13
BanI G'GyrC_C
Cuts at: 0 612 714
Size: 612 102
BanII G_rGCy'C
Cuts at: 0 74 217 236 562 714
Size: 74 143 19 326 152
Fragments arranged by size:
326 152 143 74 19
BbsI GAAGACnn'nnnn_
Cuts at: 0 593 714
Size: 593 121
BglI GCCn_nnn'nGGC
Cuts at: 0 281 714
Size: 281 433
Bme1580I G_kGCm'C
Cuts at: 0 41 74 217 236 562 617 714
Size: 41 33 143 19 326 55 97
Fragments arranged by size:
326 143 97 55 41 33 19
BmrI ACTGGGnnnn_n'
Cuts at: 0 540 714
Size: 540 174
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 643 714
Size: 643 71
BsaAI yAC'GTr
Cuts at: 0 258 714
Size: 258 456
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 424 454 714
Size: 424 30 260
Fragments arranged by size:
424 260 30
BseYI C'CCAG_C
Cuts at: 0 84 320 714
Size: 84 236 394
Fragments arranged by size:
394 236 84
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 172 445 714
Size: 172 273 269
Fragments arranged by size:
273 269 172
BsiEI CG_ry'CG
Cuts at: 0 337 714
Size: 337 377
Bsp1286I G_dGCh'C
Cuts at: 0 41 74 217 236 562 617 714
Size: 41 33 143 19 326 55 97
Fragments arranged by size:
326 143 97 55 41 33 19
BspHI T'CATG_A
Cuts at: 0 695 714
Size: 695 19
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 137 144 301 467 671 714
Size: 137 7 157 166 204 43
Fragments arranged by size:
204 166 157 137 43 7
BsrFI r'CCGG_y
Cuts at: 0 92 332 418 528 714
Size: 92 240 86 110 186
Fragments arranged by size:
240 186 110 92 86
BsrGI T'GTAC_A
Cuts at: 0 263 714
Size: 263 451
BssSI C'ACGA_G
Cuts at: 0 34 424 714
Size: 34 390 290
Fragments arranged by size:
390 290 34
BstAPI GCAn_nnn'nTGC
Cuts at: 0 258 714
Size: 258 456
Bsu36I CC'TnA_GG
Cuts at: 0 608 714
Size: 608 106
BtgI C'CryG_G
Cuts at: 0 554 674 714
Size: 554 120 40
Fragments arranged by size:
554 120 40
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 494 666 714
Size: 494 172 48
Fragments arranged by size:
494 172 48
EaeI y'GGCC_r
Cuts at: 0 334 420 671 714
Size: 334 86 251 43
Fragments arranged by size:
334 251 86 43
EagI C'GGCC_G
Cuts at: 0 334 714
Size: 334 380
EarI CTCTTCn'nnn_
Cuts at: 0 622 714
Size: 622 92
EcoO109I rG'GnC_Cy
Cuts at: 0 70 214 232 559 714
Size: 70 144 18 327 155
Fragments arranged by size:
327 155 144 70 18
HaeII r_GCGC'y
Cuts at: 0 85 321 384 392 714
Size: 85 236 63 8 322
Fragments arranged by size:
322 236 85 63 8
HindIII A'AGCT_T
Cuts at: 0 619 714
Size: 619 95
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 143 714
Size: 143 571
MspA1I CmG'CkG
Cuts at: 0 15 48 57 638 714
Size: 15 33 9 581 76
Fragments arranged by size:
581 76 33 15 9
NaeI GCC'GGC
Cuts at: 0 420 714
Size: 420 294
NcoI C'CATG_G
Cuts at: 0 554 714
Size: 554 160
NgoMIV G'CCGG_C
Cuts at: 0 418 714
Size: 418 296
NspI r_CATG'y
Cuts at: 0 100 714
Size: 100 614
PspOMI G'GGCC_C
Cuts at: 0 70 213 232 558 714
Size: 70 143 19 326 156
Fragments arranged by size:
326 156 143 70 19
SapI GCTCTTCn'nnn_
Cuts at: 0 622 714
Size: 622 92
ScaI AGT'ACT
Cuts at: 0 430 714
Size: 430 284
SfcI C'TryA_G
Cuts at: 0 384 714
Size: 384 330
SmaI CCC'GGG
Cuts at: 0 219 714
Size: 219 495
SmlI C'TyrA_G
Cuts at: 0 622 714
Size: 622 92
SphI G_CATG'C
Cuts at: 0 100 714
Size: 100 614
StyI C'CwwG_G
Cuts at: 0 66 554 714
Size: 66 488 160
Fragments arranged by size:
488 160 66
TatI w'GTAC_w
Cuts at: 0 104 263 428 714
Size: 104 159 165 286
Fragments arranged by size:
286 165 159 104
XmaI C'CCGG_G
Cuts at: 0 217 714
Size: 217 497
XmnI GAAnn'nnTTC
Cuts at: 0 487 714
Size: 487 227
Enzymes that do cut:
AarI AfeI AlwNI ApaI AvaI BanI BanII BbsI
BglI Bme1580I BmrI BpuEI BsaAI BsaXI BseYI BsgI
BsiEI Bsp1286I BspHI BspMI BsrFI BsrGI BssSI BstAPI
Bsu36I BtgI BtgZI EaeI EagI EarI EcoO109I HaeII
HindIII MmeI MspA1I NaeI NcoI NgoMIV NspI PspOMI
SapI ScaI SfcI SmaI SmlI SphI StyI TatI
XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BbeI BbvCI BcgI BciVI
BclI BfrBI BglII BlpI BmgBI BmtI BplI BpmI
Bpu10I BsaI BsaBI BsaHI BsaWI BseRI BsiHKAI BsiWI
BsmI BsmBI BspEI BsrBI BsrDI BssHII BstBI BstEII
BstXI BstYI BstZ17I BtsI ClaI CspCI DraI DraIII
DrdI EciI EcoICRI Eco57MI EcoNI EcoRI EcoRV FalI
FseI FspI FspAI Hin4I HincII HpaI KasI KpnI
MfeI MluI MscI MslI NarI NdeI NheI NotI
NruI NsiI PacI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PstI
PvuI PvuII RsrII SacI SacII SalI SanDI SbfI
SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SrfI
SspI StuI SwaI TaqII TstI Tth111I XbaI XcmI
XhoI ZraI