Restriction map for
pFN21AE3586
(Linear) (Six-base) MAPSORT of: KIEE3586.seq Check: 7715 from: 1 to: 615
KIEE3586.orf
With 182 enzymes: SgfI *
March 22, 2012 16:37 ..
AfeI AGC'GCT
Cuts at: 0 328 615
Size: 328 287
AflIII A'CryG_T
Cuts at: 0 117 615
Size: 117 498
AlwNI CAG_nnn'CTG
Cuts at: 0 184 615
Size: 184 431
ApaI G_GGCC'C
Cuts at: 0 18 615
Size: 18 597
BanII G_rGCy'C
Cuts at: 0 18 615
Size: 18 597
BbsI GAAGACnn'nnnn_
Cuts at: 0 60 416 615
Size: 60 356 199
Fragments arranged by size:
356 199 60
Bme1580I G_kGCm'C
Cuts at: 0 18 615
Size: 18 597
BmrI ACTGGGnnnn_n'
Cuts at: 0 240 278 615
Size: 240 38 337
Fragments arranged by size:
337 240 38
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 441 615
Size: 441 174
BsaAI yAC'GTr
Cuts at: 0 138 615
Size: 138 477
BsaWI w'CCGG_w
Cuts at: 0 95 615
Size: 95 520
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 295 325 370 400 615
Size: 295 30 45 30 215
Fragments arranged by size:
295 215 45 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 238 615
Size: 238 377
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 142 615
Size: 142 473
Bsp1286I G_dGCh'C
Cuts at: 0 18 615
Size: 18 597
BspEI T'CCGG_A
Cuts at: 0 95 615
Size: 95 520
BsrFI r'CCGG_y
Cuts at: 0 278 615
Size: 278 337
BstAPI GCAn_nnn'nTGC
Cuts at: 0 184 615
Size: 184 431
BstXI CCAn_nnnn'nTGG
Cuts at: 0 572 615
Size: 572 43
Bsu36I CC'TnA_GG
Cuts at: 0 464 615
Size: 464 151
BtgI C'CryG_G
Cuts at: 0 188 615
Size: 188 427
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 519 615
Size: 519 96
BtsI GCAGTG_nn'
Cuts at: 0 277 615
Size: 277 338
DraIII CAC_nnn'GTG
Cuts at: 0 174 615
Size: 174 441
EaeI y'GGCC_r
Cuts at: 0 44 615
Size: 44 571
EarI CTCTTCn'nnn_
Cuts at: 0 484 565 615
Size: 484 81 50
Fragments arranged by size:
484 81 50
EcoNI CCTnn'n_nnAGG
Cuts at: 0 349 615
Size: 349 266
EcoO109I rG'GnC_Cy
Cuts at: 0 15 615
Size: 15 600
HaeII r_GCGC'y
Cuts at: 0 330 615
Size: 330 285
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 295 327 615
Size: 295 32 288
Fragments arranged by size:
295 288 32
MslI CAynn'nnrTG
Cuts at: 0 118 404 615
Size: 118 286 211
Fragments arranged by size:
286 211 118
MspA1I CmG'CkG
Cuts at: 0 49 126 234 266 416 615
Size: 49 77 108 32 150 199
Fragments arranged by size:
199 150 108 77 49 32
NcoI C'CATG_G
Cuts at: 0 188 615
Size: 188 427
NspI r_CATG'y
Cuts at: 0 121 615
Size: 121 494
PasI CC'CwG_GG
Cuts at: 0 345 615
Size: 345 270
PciI A'CATG_T
Cuts at: 0 117 615
Size: 117 498
PspOMI G'GGCC_C
Cuts at: 0 14 615
Size: 14 601
PstI C_TGCA'G
Cuts at: 0 40 615
Size: 40 575
PvuII CAG'CTG
Cuts at: 0 126 234 615
Size: 126 108 381
Fragments arranged by size:
381 126 108
SexAI A'CCwGG_T
Cuts at: 0 209 615
Size: 209 406
SfcI C'TryA_G
Cuts at: 0 36 615
Size: 36 579
SmlI C'TyrA_G
Cuts at: 0 420 615
Size: 420 195
StyI C'CwwG_G
Cuts at: 0 188 251 615
Size: 188 63 364
Fragments arranged by size:
364 188 63
TatI w'GTAC_w
Cuts at: 0 197 615
Size: 197 418
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 444 476 545 577 615
Size: 444 32 69 32 38
Fragments arranged by size:
444 69 38 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 259 615
Size: 259 356
Enzymes that do cut:
AfeI AflIII AlwNI ApaI BanII BbsI Bme1580I BmrI
BpuEI BsaAI BsaWI BsaXI BseRI BsgI Bsp1286I BspEI
BsrFI BstAPI BstXI Bsu36I BtgI BtgZI BtsI DraIII
EaeI EarI EcoNI EcoO109I HaeII Hin4I MslI MspA1I
NcoI NspI PasI PciI PspOMI PstI PvuII SexAI
SfcI SmlI StyI TatI TstI Tth111I
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AflII AgeI
AhdI AleI AlfI AloI ApaLI ApoI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BbeI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI BmgBI
BmtI BplI BpmI Bpu10I BsaI BsaBI BsaHI BseYI
BsiEI BsiHKAI BsiWI BsmI BsmBI BspHI BspMI BsrBI
BsrDI BsrGI BssHII BssSI BstBI BstEII BstYI BstZ17I
ClaI CspCI DraI DrdI EagI EciI EcoICRI Eco57MI
EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI NaeI
NarI NdeI NgoMIV NheI NotI NruI NsiI PacI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspXI PsrI PvuI RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII
XbaI XcmI XhoI XmaI XmnI ZraI