Restriction map for
pFN21AE3585
(Linear) (Six-base) MAPSORT of: KIEE3585.seq Check: 4857 from: 1 to: 1593
KIEE3585.orf
With 182 enzymes: SgfI *
March 22, 2012 16:36 ..
AgeI A'CCGG_T
Cuts at: 0 600 1593
Size: 600 993
AleI CACnn'nnGTG
Cuts at: 0 601 1593
Size: 601 992
AlwNI CAG_nnn'CTG
Cuts at: 0 1561 1593
Size: 1561 32
AvaI C'yCGr_G
Cuts at: 0 590 875 1239 1331 1593
Size: 590 285 364 92 262
Fragments arranged by size:
590 364 285 262 92
BanI G'GyrC_C
Cuts at: 0 3 381 1030 1593
Size: 3 378 649 563
Fragments arranged by size:
649 563 378 3
BanII G_rGCy'C
Cuts at: 0 1180 1593
Size: 1180 413
BbeI G_GCGC'C
Cuts at: 0 7 1593
Size: 7 1586
BbsI GAAGACnn'nnnn_
Cuts at: 0 547 612 1593
Size: 547 65 981
Fragments arranged by size:
981 547 65
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 363 397 1593
Size: 363 34 1196
Fragments arranged by size:
1196 363 34
BglII A'GATC_T
Cuts at: 0 1355 1593
Size: 1355 238
BmrI ACTGGGnnnn_n'
Cuts at: 0 1505 1593
Size: 1505 88
BmtI G_CTAG'C
Cuts at: 0 911 1593
Size: 911 682
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 426 1593
Size: 426 1167
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 101 1593
Size: 101 1492
Bpu10I CC'TnA_GC
Cuts at: 0 656 1088 1593
Size: 656 432 505
Fragments arranged by size:
656 505 432
BsaBI GATnn'nnATC
Cuts at: 0 640 1593
Size: 640 953
BsaHI Gr'CG_yC
Cuts at: 0 4 1593
Size: 4 1589
BsaWI w'CCGG_w
Cuts at: 0 59 600 1125 1593
Size: 59 541 525 468
Fragments arranged by size:
541 525 468 59
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 203 233 706 736 1593
Size: 203 30 473 30 857
Fragments arranged by size:
857 473 203 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 343 841 844 1144 1159 1201 1366 1593
Size: 343 498 3 300 15 42 165 227
Fragments arranged by size:
498 343 300 227 165 42 15 3
BseYI C'CCAG_C
Cuts at: 0 26 362 678 1015 1106 1171 1593
Size: 26 336 316 337 91 65 422
Fragments arranged by size:
422 337 336 316 91 65 26
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 722 1593
Size: 722 871
BsiHKAI G_wGCw'C
Cuts at: 0 657 1238 1526 1593
Size: 657 581 288 67
Fragments arranged by size:
657 581 288 67
BsmBI CGTCTCn'nnnn_
Cuts at: 0 790 1593
Size: 790 803
Bsp1286I G_dGCh'C
Cuts at: 0 657 1180 1238 1526 1593
Size: 657 523 58 288 67
Fragments arranged by size:
657 523 288 67 58
BsrDI GCAATG_nn'
Cuts at: 0 1464 1593
Size: 1464 129
BsrFI r'CCGG_y
Cuts at: 0 600 1593
Size: 600 993
BssSI C'ACGA_G
Cuts at: 0 693 1593
Size: 693 900
BstEII G'GTnAC_C
Cuts at: 0 243 1593
Size: 243 1350
BstXI CCAn_nnnn'nTGG
Cuts at: 0 195 1540 1593
Size: 195 1345 53
Fragments arranged by size:
1345 195 53
BstYI r'GATC_y
Cuts at: 0 300 358 1355 1593
Size: 300 58 997 238
Fragments arranged by size:
997 300 238 58
Bsu36I CC'TnA_GG
Cuts at: 0 452 1593
Size: 452 1141
BtgI C'CryG_G
Cuts at: 0 385 1593
Size: 385 1208
BtsI GCAGTG_nn'
Cuts at: 0 1215 1230 1305 1371 1437 1551 1593
Size: 1215 15 75 66 66 114 42
Fragments arranged by size:
1215 114 75 66 66 42 15
ClaI AT'CG_AT
Cuts at: 0 251 1593
Size: 251 1342
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1446 1481 1593
Size: 1446 35 112
Fragments arranged by size:
1446 112 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 241 1593
Size: 241 1352
EaeI y'GGCC_r
Cuts at: 0 39 1467 1593
Size: 39 1428 126
Fragments arranged by size:
1428 126 39
EarI CTCTTCn'nnn_
Cuts at: 0 320 788 1130 1136 1154 1593
Size: 320 468 342 6 18 439
Fragments arranged by size:
468 439 342 320 18 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 979 1593
Size: 979 614
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 101 1593
Size: 101 1492
EcoO109I rG'GnC_Cy
Cuts at: 0 339 1593
Size: 339 1254
EcoRV GAT'ATC
Cuts at: 0 123 1593
Size: 123 1470
HaeII r_GCGC'y
Cuts at: 0 7 1005 1499 1593
Size: 7 998 494 94
Fragments arranged by size:
998 494 94 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 706 708 738 740 1222 1254 1378 1410
Size: 706 2 30 2 482 32 124 32
Cuts at: 1410 1593
Size: 183
Fragments arranged by size:
706 482 183 124 32 32 30 2
2
KasI G'GCGC_C
Cuts at: 0 3 1593
Size: 3 1590
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 692 956 1593
Size: 692 264 637
Fragments arranged by size:
692 637 264
MscI TGG'CCA
Cuts at: 0 41 1469 1593
Size: 41 1428 124
Fragments arranged by size:
1428 124 41
MslI CAynn'nnrTG
Cuts at: 0 212 601 1593
Size: 212 389 992
Fragments arranged by size:
992 389 212
MspA1I CmG'CkG
Cuts at: 0 30 307 706 1451 1473 1593
Size: 30 277 399 745 22 120
Fragments arranged by size:
745 399 277 120 30 22
NarI GG'CG_CC
Cuts at: 0 4 1593
Size: 4 1589
NcoI C'CATG_G
Cuts at: 0 385 1593
Size: 385 1208
NheI G'CTAG_C
Cuts at: 0 907 1593
Size: 907 686
PflMI CCAn_nnn'nTGG
Cuts at: 0 771 1593
Size: 771 822
PstI C_TGCA'G
Cuts at: 0 1557 1593
Size: 1557 36
PvuII CAG'CTG
Cuts at: 0 307 706 1593
Size: 307 399 887
Fragments arranged by size:
887 399 307
ScaI AGT'ACT
Cuts at: 0 103 1593
Size: 103 1490
SfcI C'TryA_G
Cuts at: 0 788 1553 1593
Size: 788 765 40
Fragments arranged by size:
788 765 40
SfoI GGC'GCC
Cuts at: 0 5 1593
Size: 5 1588
SgrAI Cr'CCGG_yG
Cuts at: 0 600 1593
Size: 600 993
SmaI CCC'GGG
Cuts at: 0 1333 1593
Size: 1333 260
SrfI GCCC'GGGC
Cuts at: 0 1333 1593
Size: 1333 260
StyI C'CwwG_G
Cuts at: 0 194 385 686 1010 1422 1593
Size: 194 191 301 324 412 171
Fragments arranged by size:
412 324 301 194 191 171
TatI w'GTAC_w
Cuts at: 0 101 179 404 881 1593
Size: 101 78 225 477 712
Fragments arranged by size:
712 477 225 101 78
XbaI T'CTAG_A
Cuts at: 0 297 1593
Size: 297 1296
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 34 1593
Size: 34 1559
XmaI C'CCGG_G
Cuts at: 0 1331 1593
Size: 1331 262
XmnI GAAnn'nnTTC
Cuts at: 0 169 1593
Size: 169 1424
Enzymes that do cut:
AgeI AleI AlwNI AvaI BanI BanII BbeI BbsI
BcgI BglII BmrI BmtI BplI BpmI Bpu10I BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI
Bsp1286I BsrDI BsrFI BssSI BstEII BstXI BstYI Bsu36I
BtgI BtsI ClaI CspCI DrdI EaeI EarI EciI
Eco57MI EcoO109I EcoRV HaeII Hin4I KasI MmeI MscI
MslI MspA1I NarI NcoI NheI PflMI PstI PvuII
ScaI SfcI SfoI SgrAI SmaI SrfI StyI TatI
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AhdI AlfI AloI ApaI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BbvCI BciVI BclI
BfrBI BglI BlpI Bme1580I BmgBI BpuEI BsaI BsaAI
BsiEI BsiWI BsmI BspEI BspHI BspMI BsrBI BsrGI
BssHII BstAPI BstBI BstZ17I BtgZI DraI DraIII EagI
EcoICRI EcoNI EcoRI FalI FseI FspI FspAI HincII
HindIII HpaI KpnI MfeI MluI NaeI NdeI NgoMIV
NotI NruI NsiI NspI PacI PasI PciI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PsrI PvuI RsrII SacI SacII SalI SanDI SapI
SbfI SexAI SfiI SgfI SmlI SnaBI SpeI SphI
SspI StuI SwaI TaqII TstI Tth111I XhoI ZraI