Restriction map for
pFN21AE3584
(Linear) (Six-base) MAPSORT of: KIEE3584.seq Check: 5402 from: 1 to: 1299
KIEE3584.orf
With 182 enzymes: SgfI *
December 27, 2011 16:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 599 1299
Size: 599 700
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 89 251 418 1299
Size: 89 162 167 881
Fragments arranged by size:
881 167 162 89
AflIII A'CryG_T
Cuts at: 0 143 419 515 1280 1299
Size: 143 276 96 765 19
Fragments arranged by size:
765 276 143 96 19
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 162 1034 1299
Size: 162 872 265
Fragments arranged by size:
872 265 162
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 406 438 1299
Size: 406 32 861
Fragments arranged by size:
861 406 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1098 1299
Size: 1098 201
ApoI r'AATT_y
Cuts at: 0 333 1299
Size: 333 966
BamHI G'GATC_C
Cuts at: 0 382 1299
Size: 382 917
BanI G'GyrC_C
Cuts at: 0 46 131 1025 1299
Size: 46 85 894 274
Fragments arranged by size:
894 274 85 46
BanII G_rGCy'C
Cuts at: 0 1113 1299
Size: 1113 186
BbsI GAAGACnn'nnnn_
Cuts at: 0 239 722 1299
Size: 239 483 577
Fragments arranged by size:
577 483 239
BbvCI CC'TCA_GC
Cuts at: 0 1082 1299
Size: 1082 217
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 202 236 1299
Size: 202 34 1063
Fragments arranged by size:
1063 202 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1256 1299
Size: 1256 43
BclI T'GATC_A
Cuts at: 0 257 1299
Size: 257 1042
BglII A'GATC_T
Cuts at: 0 477 732 1299
Size: 477 255 567
Fragments arranged by size:
567 477 255
Bme1580I G_kGCm'C
Cuts at: 0 1127 1299
Size: 1127 172
BmgBI CAC'GTC
Cuts at: 0 726 798 1299
Size: 726 72 501
Fragments arranged by size:
726 501 72
BmrI ACTGGGnnnn_n'
Cuts at: 0 96 1299
Size: 96 1203
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1132 1299
Size: 1132 167
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 230 332 875 994 1299
Size: 230 102 543 119 305
Fragments arranged by size:
543 305 230 119 102
Bpu10I CC'TnA_GC
Cuts at: 0 1082 1299
Size: 1082 217
BsaI GGTCTCn'nnnn_
Cuts at: 0 496 739 1299
Size: 496 243 560
Fragments arranged by size:
560 496 243
BsaAI yAC'GTr
Cuts at: 0 420 680 1190 1218 1281 1299
Size: 420 260 510 28 63 18
Fragments arranged by size:
510 420 260 63 28 18
BsaBI GATnn'nnATC
Cuts at: 0 284 1299
Size: 284 1015
BsaWI w'CCGG_w
Cuts at: 0 1157 1299
Size: 1157 142
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 305 335 1165 1195 1299
Size: 305 30 830 30 104
Fragments arranged by size:
830 305 104 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 991 1012 1299
Size: 991 21 287
Fragments arranged by size:
991 287 21
BseYI C'CCAG_C
Cuts at: 0 916 1299
Size: 916 383
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 736 1135 1299
Size: 736 399 164
Fragments arranged by size:
736 399 164
BsiWI C'GTAC_G
Cuts at: 0 1190 1299
Size: 1190 109
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1062 1299
Size: 1062 237
Bsp1286I G_dGCh'C
Cuts at: 0 1113 1127 1299
Size: 1113 14 172
Fragments arranged by size:
1113 172 14
BspEI T'CCGG_A
Cuts at: 0 1157 1299
Size: 1157 142
BspHI T'CATG_A
Cuts at: 0 941 1299
Size: 941 358
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 94 599 1299
Size: 94 505 700
Fragments arranged by size:
700 505 94
BsrBI CCG'CTC
Cuts at: 0 1116 1299
Size: 1116 183
BsrDI GCAATG_nn'
Cuts at: 0 180 218 1299
Size: 180 38 1081
Fragments arranged by size:
1081 180 38
BsrGI T'GTAC_A
Cuts at: 0 518 1299
Size: 518 781
BstAPI GCAn_nnn'nTGC
Cuts at: 0 622 1299
Size: 622 677
BstXI CCAn_nnnn'nTGG
Cuts at: 0 684 972 1299
Size: 684 288 327
Fragments arranged by size:
684 327 288
BstYI r'GATC_y
Cuts at: 0 382 445 477 732 1001 1299
Size: 382 63 32 255 269 298
Fragments arranged by size:
382 298 269 255 63 32
BtgI C'CryG_G
Cuts at: 0 441 1271 1299
Size: 441 830 28
Fragments arranged by size:
830 441 28
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 371 1299
Size: 371 928
BtsI GCAGTG_nn'
Cuts at: 0 374 1164 1299
Size: 374 790 135
Fragments arranged by size:
790 374 135
DraIII CAC_nnn'GTG
Cuts at: 0 90 1299
Size: 90 1209
EarI CTCTTCn'nnn_
Cuts at: 0 394 664 1299
Size: 394 270 635
Fragments arranged by size:
635 394 270
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 89 230 251 332 418 875 994 1299
Size: 89 141 21 81 86 457 119 305
Fragments arranged by size:
457 305 141 119 89 86 81 21
EcoO109I rG'GnC_Cy
Cuts at: 0 492 1143 1299
Size: 492 651 156
Fragments arranged by size:
651 492 156
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 430 462 559 591 658 690 1299
Size: 430 32 97 32 67 32 609
Fragments arranged by size:
609 430 97 67 32 32 32
HincII GTy'rAC
Cuts at: 0 705 1227 1299
Size: 705 522 72
Fragments arranged by size:
705 522 72
MluI A'CGCG_T
Cuts at: 0 143 1299
Size: 143 1156
MslI CAynn'nnrTG
Cuts at: 0 173 835 940 1016 1299
Size: 173 662 105 76 283
Fragments arranged by size:
662 283 173 105 76
MspA1I CmG'CkG
Cuts at: 0 1008 1130 1299
Size: 1008 122 169
Fragments arranged by size:
1008 169 122
NcoI C'CATG_G
Cuts at: 0 441 1271 1299
Size: 441 830 28
Fragments arranged by size:
830 441 28
NspI r_CATG'y
Cuts at: 0 519 1299
Size: 519 780
PasI CC'CwG_GG
Cuts at: 0 36 1299
Size: 36 1263
PciI A'CATG_T
Cuts at: 0 515 1299
Size: 515 784
PfoI T'CCnGG_A
Cuts at: 0 246 1299
Size: 246 1053
PmlI CAC'GTG
Cuts at: 0 420 1281 1299
Size: 420 861 18
Fragments arranged by size:
861 420 18
PpuMI rG'GwC_Cy
Cuts at: 0 492 1299
Size: 492 807
PvuII CAG'CTG
Cuts at: 0 1130 1299
Size: 1130 169
SexAI A'CCwGG_T
Cuts at: 0 767 1299
Size: 767 532
SfcI C'TryA_G
Cuts at: 0 56 1299
Size: 56 1243
StyI C'CwwG_G
Cuts at: 0 441 1271 1287 1299
Size: 441 830 16 12
Fragments arranged by size:
830 441 16 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 78 1299
Size: 78 1221
TatI w'GTAC_w
Cuts at: 0 518 1299
Size: 518 781
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 320 352 1267 1299
Size: 320 32 915 32
Fragments arranged by size:
915 320 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 448 1285 1299
Size: 448 837 14
Fragments arranged by size:
837 448 14
XmnI GAAnn'nnTTC
Cuts at: 0 479 1299
Size: 479 820
Enzymes that do cut:
AarI AcuI AflIII AlfI AloI AlwNI ApoI BamHI
BanI BanII BbsI BbvCI BcgI BciVI BclI BglII
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BsaI BsaAI
BsaBI BsaWI BsaXI BseRI BseYI BsgI BsiWI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrGI BstAPI
BstXI BstYI BtgI BtgZI BtsI DraIII EarI Eco57MI
EcoO109I Hin4I HincII MluI MslI MspA1I NcoI NspI
PasI PciI PfoI PmlI PpuMI PvuII SexAI SfcI
StyI TaqII TatI TstI XcmI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AleI ApaI ApaLI AscI AseI AsiSI AvaI AvrII
BaeI BbeI BfrBI BglI BlpI BmtI BpuEI BsaHI
BsiEI BsiHKAI BsmI BsrFI BssHII BssSI BstBI BstEII
BstZ17I Bsu36I ClaI CspCI DraI DrdI EaeI EagI
EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI
FspAI HaeII HindIII HpaI KasI KpnI MfeI MmeI
MscI NaeI NarI NdeI NgoMIV NheI NotI NruI
NsiI PacI PflMI PmeI PpiI PshAI PsiI PspOMI
PspXI PsrI PstI PvuI RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
SwaI Tth111I XbaI XhoI XmaI ZraI