Restriction map for
pFN21AE3583
(Linear) (Six-base) MAPSORT of: KIEE3583.seq Check: 2036 from: 1 to: 1254
KIEE3583.orf
With 182 enzymes: SgfI *
December 27, 2011 16:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 783 1254
Size: 783 471
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 425 472 1254
Size: 425 47 782
Fragments arranged by size:
782 425 47
AlwNI CAG_nnn'CTG
Cuts at: 0 1117 1254
Size: 1117 137
ApaI G_GGCC'C
Cuts at: 0 94 730 1254
Size: 94 636 524
Fragments arranged by size:
636 524 94
ApoI r'AATT_y
Cuts at: 0 211 994 1254
Size: 211 783 260
Fragments arranged by size:
783 260 211
AvaI C'yCGr_G
Cuts at: 0 596 1254
Size: 596 658
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 697 730 1254
Size: 697 33 524
Fragments arranged by size:
697 524 33
BamHI G'GATC_C
Cuts at: 0 860 951 1254
Size: 860 91 303
Fragments arranged by size:
860 303 91
BanII G_rGCy'C
Cuts at: 0 94 313 548 569 697 730 1254
Size: 94 219 235 21 128 33 524
Fragments arranged by size:
524 235 219 128 94 33 21
BbsI GAAGACnn'nnnn_
Cuts at: 0 640 1088 1254
Size: 640 448 166
Fragments arranged by size:
640 448 166
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 784 818 1254
Size: 784 34 436
Fragments arranged by size:
784 436 34
BclI T'GATC_A
Cuts at: 0 897 1254
Size: 897 357
BglI GCCn_nnn'nGGC
Cuts at: 0 388 623 975 1254
Size: 388 235 352 279
Fragments arranged by size:
388 352 279 235
Bme1580I G_kGCm'C
Cuts at: 0 94 730 1254
Size: 94 636 524
Fragments arranged by size:
636 524 94
BmgBI CAC'GTC
Cuts at: 0 423 1254
Size: 423 831
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 622 1254
Size: 622 632
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 130 172 181 298 889 1254
Size: 130 42 9 117 591 365
Fragments arranged by size:
591 365 130 117 42 9
Bpu10I CC'TnA_GC
Cuts at: 0 32 95 346 1254
Size: 32 63 251 908
Fragments arranged by size:
908 251 63 32
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 355 1254
Size: 355 899
BsaBI GATnn'nnATC
Cuts at: 0 13 583 1254
Size: 13 570 671
Fragments arranged by size:
671 570 13
BsaHI Gr'CG_yC
Cuts at: 0 602 1254
Size: 602 652
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 103 133 1228 1254
Size: 103 30 1095 26
Fragments arranged by size:
1095 103 30 26
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 19 244 1081 1254
Size: 19 225 837 173
Fragments arranged by size:
837 225 173 19
BseYI C'CCAG_C
Cuts at: 0 45 1254
Size: 45 1209
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 187 1254
Size: 187 1067
BsiEI CG_ry'CG
Cuts at: 0 360 1254
Size: 360 894
BsiHKAI G_wGCw'C
Cuts at: 0 352 548 569 1254
Size: 352 196 21 685
Fragments arranged by size:
685 352 196 21
BsmI GAATG_Cn'
Cuts at: 0 676 774 1254
Size: 676 98 480
Fragments arranged by size:
676 480 98
Bsp1286I G_dGCh'C
Cuts at: 0 94 313 352 548 569 697 730 1254
Size: 94 219 39 196 21 128 33 524
Fragments arranged by size:
524 219 196 128 94 39 33 21
BspHI T'CATG_A
Cuts at: 0 452 1254
Size: 452 802
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 783 1254
Size: 783 471
BsrBI CCG'CTC
Cuts at: 0 552 1254
Size: 552 702
BsrDI GCAATG_nn'
Cuts at: 0 414 1254
Size: 414 840
BsrFI r'CCGG_y
Cuts at: 0 73 1254
Size: 73 1181
BsrGI T'GTAC_A
Cuts at: 0 119 705 1254
Size: 119 586 549
Fragments arranged by size:
586 549 119
BstAPI GCAn_nnn'nTGC
Cuts at: 0 528 1254
Size: 528 726
BstYI r'GATC_y
Cuts at: 0 41 860 951 1254
Size: 41 819 91 303
Fragments arranged by size:
819 303 91 41
Bsu36I CC'TnA_GG
Cuts at: 0 101 1254
Size: 101 1153
BtgI C'CryG_G
Cuts at: 0 539 1254
Size: 539 715
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 792 1254
Size: 792 462
ClaI AT'CG_AT
Cuts at: 0 356 587 1254
Size: 356 231 667
Fragments arranged by size:
667 356 231
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 635 670 1254
Size: 635 35 584
Fragments arranged by size:
635 584 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 78 1254
Size: 78 1176
EaeI y'GGCC_r
Cuts at: 0 758 926 967 1096 1184 1254
Size: 758 168 41 129 88 70
Fragments arranged by size:
758 168 129 88 70 41
EarI CTCTTCn'nnn_
Cuts at: 0 1015 1254
Size: 1015 239
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 742 1254
Size: 742 512
EcoICRI GAG'CTC
Cuts at: 0 546 567 1254
Size: 546 21 687
Fragments arranged by size:
687 546 21
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 130 172 181 298 889 1254
Size: 130 42 9 117 591 365
Fragments arranged by size:
591 365 130 117 42 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 205 760 1063 1186 1254
Size: 205 555 303 123 68
Fragments arranged by size:
555 303 205 123 68
EcoO109I rG'GnC_Cy
Cuts at: 0 91 105 114 726 727 1254
Size: 91 14 9 612 1 527
Fragments arranged by size:
612 527 91 14 9 1
FspI TGC'GCA
Cuts at: 0 340 1254
Size: 340 914
FspAI rTGC'GCAy
Cuts at: 0 340 1254
Size: 340 914
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1111 1143 1254
Size: 1111 32 111
Fragments arranged by size:
1111 111 32
HincII GTy'rAC
Cuts at: 0 1074 1254
Size: 1074 180
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 350 1254
Size: 350 904
MscI TGG'CCA
Cuts at: 0 760 928 1186 1254
Size: 760 168 258 68
Fragments arranged by size:
760 258 168 68
MslI CAynn'nnrTG
Cuts at: 0 19 407 454 1254
Size: 19 388 47 800
Fragments arranged by size:
800 388 47 19
MspA1I CmG'CkG
Cuts at: 0 258 673 1254
Size: 258 415 581
Fragments arranged by size:
581 415 258
PfoI T'CCnGG_A
Cuts at: 0 158 740 1254
Size: 158 582 514
Fragments arranged by size:
582 514 158
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 139 171 1254
Size: 139 32 1083
Fragments arranged by size:
1083 139 32
PpuMI rG'GwC_Cy
Cuts at: 0 105 114 1254
Size: 105 9 1140
Fragments arranged by size:
1140 105 9
PspOMI G'GGCC_C
Cuts at: 0 90 726 1254
Size: 90 636 528
Fragments arranged by size:
636 528 90
PstI C_TGCA'G
Cuts at: 0 143 1254
Size: 143 1111
PvuI CG_AT'CG
Cuts at: 0 360 1254
Size: 360 894
PvuII CAG'CTG
Cuts at: 0 673 1254
Size: 673 581
SacI G_AGCT'C
Cuts at: 0 548 569 1254
Size: 548 21 685
Fragments arranged by size:
685 548 21
SfcI C'TryA_G
Cuts at: 0 139 1254
Size: 139 1115
SmlI C'TyrA_G
Cuts at: 0 370 1254
Size: 370 884
StyI C'CwwG_G
Cuts at: 0 761 1187 1254
Size: 761 426 67
Fragments arranged by size:
761 426 67
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 709 1254
Size: 709 545
TatI w'GTAC_w
Cuts at: 0 119 705 1254
Size: 119 586 549
Fragments arranged by size:
586 549 119
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 936 968 1254
Size: 936 32 286
Fragments arranged by size:
936 286 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 384 1254
Size: 384 870
Enzymes that do cut:
AarI AlfI AlwNI ApaI ApoI AvaI BaeI BamHI
BanII BbsI BcgI BclI BglI Bme1580I BmgBI BplI
BpmI Bpu10I BpuEI BsaBI BsaHI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BstAPI BstYI Bsu36I BtgI BtgZI
ClaI CspCI DrdI EaeI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I FspI FspAI Hin4I HincII MmeI MscI
MslI MspA1I PfoI PpiI PpuMI PspOMI PstI PvuI
PvuII SacI SfcI SmlI StyI TaqII TatI TstI
XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AloI ApaLI AscI AseI AsiSI
AvrII BanI BbeI BbvCI BciVI BfrBI BglII BlpI
BmrI BmtI BsaI BsaAI BsaWI BsiWI BsmBI BspEI
BssHII BssSI BstBI BstEII BstXI BstZ17I BtsI DraI
DraIII EagI EcoRI EcoRV FalI FseI HaeII HindIII
HpaI KasI KpnI MfeI MluI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NsiI NspI PacI
PasI PciI PflMI PmeI PmlI PshAI PsiI PspXI
PsrI RsrII SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI StuI SwaI Tth111I XbaI XhoI
XmaI XmnI ZraI