Restriction map for
pFN21AE3567
(Linear) (Six-base) MAPSORT of: KIEE3567.seq Check: 2813 from: 1 to: 3321
KIEE3567.orf
With 182 enzymes: SgfI *
March 22, 2012 16:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 539 3321
Size: 539 2782
AccI GT'mk_AC
Cuts at: 0 128 3321
Size: 128 3193
AclI AA'CG_TT
Cuts at: 0 694 725 2690 3321
Size: 694 31 1965 631
Fragments arranged by size:
1965 694 631 31
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 194 1183 3147 3171 3321
Size: 194 989 1964 24 150
Fragments arranged by size:
1964 989 194 150 24
AflII C'TTAA_G
Cuts at: 0 2250 3321
Size: 2250 1071
AhdI GACnn_n'nnGTC
Cuts at: 0 750 3321
Size: 750 2571
AleI CACnn'nnGTG
Cuts at: 0 967 1300 2740 3321
Size: 967 333 1440 581
Fragments arranged by size:
1440 967 581 333
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2954 3321
Size: 2954 367
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2546 2578 3321
Size: 2546 32 743
Fragments arranged by size:
2546 743 32
AlwNI CAG_nnn'CTG
Cuts at: 0 2682 3321
Size: 2682 639
ApaI G_GGCC'C
Cuts at: 0 1243 3321
Size: 1243 2078
ApaLI G'TGCA_C
Cuts at: 0 2786 3321
Size: 2786 535
ApoI r'AATT_y
Cuts at: 0 274 631 822 1978 2316 2522 3321
Size: 274 357 191 1156 338 206 799
Fragments arranged by size:
1156 799 357 338 274 206 191
AvaI C'yCGr_G
Cuts at: 0 1776 2271 3321
Size: 1776 495 1050
Fragments arranged by size:
1776 1050 495
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 395 428 3321
Size: 395 33 2893
Fragments arranged by size:
2893 395 33
BamHI G'GATC_C
Cuts at: 0 2883 3321
Size: 2883 438
BanI G'GyrC_C
Cuts at: 0 24 323 527 1372 2280 3321
Size: 24 299 204 845 908 1041
Fragments arranged by size:
1041 908 845 299 204 24
BanII G_rGCy'C
Cuts at: 0 1243 1783 2087 2519 2925 3321
Size: 1243 540 304 432 406 396
Fragments arranged by size:
1243 540 432 406 396 304
BbeI G_GCGC'C
Cuts at: 0 28 3321
Size: 28 3293
BbsI GAAGACnn'nnnn_
Cuts at: 0 446 1154 1994 3321
Size: 446 708 840 1327
Fragments arranged by size:
1327 840 708 446
BbvCI CC'TCA_GC
Cuts at: 0 2702 3321
Size: 2702 619
BclI T'GATC_A
Cuts at: 0 1871 3321
Size: 1871 1450
BglI GCCn_nnn'nGGC
Cuts at: 0 106 3321
Size: 106 3215
BglII A'GATC_T
Cuts at: 0 1343 1634 3321
Size: 1343 291 1687
Fragments arranged by size:
1687 1343 291
BlpI GC'TnA_GC
Cuts at: 0 1285 3321
Size: 1285 2036
Bme1580I G_kGCm'C
Cuts at: 0 530 1243 2285 2790 3321
Size: 530 713 1042 505 531
Fragments arranged by size:
1042 713 531 530 505
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2190 3321
Size: 2190 1131
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 280 407 1416 1900 2292 2736 2802 2939
Size: 280 127 1009 484 392 444 66 137
Cuts at: 2939 3321
Size: 382
Fragments arranged by size:
1009 484 444 392 382 280 137 127
66
Bpu10I CC'TnA_GC
Cuts at: 0 110 2702 3321
Size: 110 2592 619
Fragments arranged by size:
2592 619 110
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1492 3321
Size: 1492 1829
BsaI GGTCTCn'nnnn_
Cuts at: 0 317 1688 2076 2578 3321
Size: 317 1371 388 502 743
Fragments arranged by size:
1371 743 502 388 317
BsaHI Gr'CG_yC
Cuts at: 0 25 3061 3321
Size: 25 3036 260
Fragments arranged by size:
3036 260 25
BsaWI w'CCGG_w
Cuts at: 0 597 3321
Size: 597 2724
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 353 383 483 513 2546 2576 3321
Size: 353 30 100 30 2033 30 745
Fragments arranged by size:
2033 745 353 100 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 277 2158 2161 2164 2179 2519 2522 2596
Size: 277 1881 3 3 15 340 3 74
Cuts at: 2596 2880 3096 3166 3321
Size: 284 216 70 155
Fragments arranged by size:
1881 340 284 277 216 155 74 70
15 3 3 3
BseYI C'CCAG_C
Cuts at: 0 668 3321
Size: 668 2653
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1341 2175 3321
Size: 1341 834 1146
Fragments arranged by size:
1341 1146 834
BsiHKAI G_wGCw'C
Cuts at: 0 769 1719 2790 2925 3321
Size: 769 950 1071 135 396
Fragments arranged by size:
1071 950 769 396 135
BsmBI CGTCTCn'nnnn_
Cuts at: 0 486 1323 3321
Size: 486 837 1998
Fragments arranged by size:
1998 837 486
Bsp1286I G_dGCh'C
Cuts at: 0 530 769 1243 1719 1783 2087 2285 2519
Size: 530 239 474 476 64 304 198 234
Cuts at: 2519 2790 2925 3321
Size: 271 135 396
Fragments arranged by size:
530 476 474 396 304 271 239 234
198 135 64
BspEI T'CCGG_A
Cuts at: 0 597 3321
Size: 597 2724
BspHI T'CATG_A
Cuts at: 0 1185 2028 2925 3321
Size: 1185 843 897 396
Fragments arranged by size:
1185 897 843 396
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 539 3321
Size: 539 2782
BsrBI CCG'CTC
Cuts at: 0 1270 3321
Size: 1270 2051
BsrDI GCAATG_nn'
Cuts at: 0 747 782 3321
Size: 747 35 2539
Fragments arranged by size:
2539 747 35
BsrFI r'CCGG_y
Cuts at: 0 3 3321
Size: 3 3318
BssSI C'ACGA_G
Cuts at: 0 976 3321
Size: 976 2345
BstAPI GCAn_nnn'nTGC
Cuts at: 0 237 1096 1518 3321
Size: 237 859 422 1803
Fragments arranged by size:
1803 859 422 237
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2917 3212 3321
Size: 2917 295 109
Fragments arranged by size:
2917 295 109
BstYI r'GATC_y
Cuts at: 0 1343 1464 1634 2200 2541 2883 3208 3321
Size: 1343 121 170 566 341 342 325 113
Fragments arranged by size:
1343 566 342 341 325 170 121 113
Bsu36I CC'TnA_GG
Cuts at: 0 1678 3321
Size: 1678 1643
BtgI C'CryG_G
Cuts at: 0 1784 3321
Size: 1784 1537
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 36 3321
Size: 36 3285
BtsI GCAGTG_nn'
Cuts at: 0 794 2472 3321
Size: 794 1678 849
Fragments arranged by size:
1678 849 794
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 45 80 3321
Size: 45 35 3241
Fragments arranged by size:
3241 45 35
DraI TTT'AAA
Cuts at: 0 660 821 3321
Size: 660 161 2500
Fragments arranged by size:
2500 660 161
EaeI y'GGCC_r
Cuts at: 0 852 3321
Size: 852 2469
EarI CTCTTCn'nnn_
Cuts at: 0 181 534 2135 2156 2572 3114 3321
Size: 181 353 1601 21 416 542 207
Fragments arranged by size:
1601 542 416 353 207 181 21
EcoICRI GAG'CTC
Cuts at: 0 2923 3321
Size: 2923 398
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 194 280 407 1183 1416 1900 2292 2736
Size: 194 86 127 776 233 484 392 444
Cuts at: 2736 2802 2939 3147 3171 3321
Size: 66 137 208 24 150
Fragments arranged by size:
776 484 444 392 233 208 194 150
137 127 86 66 24
EcoO109I rG'GnC_Cy
Cuts at: 0 470 1239 3321
Size: 470 769 2082
Fragments arranged by size:
2082 769 470
EcoRI G'AATT_C
Cuts at: 0 274 3321
Size: 274 3047
EcoRV GAT'ATC
Cuts at: 0 2045 3321
Size: 2045 1276
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1653 2219 2498 2860 3321
Size: 1653 566 279 362 461
Fragments arranged by size:
1653 566 461 362 279
HaeII r_GCGC'y
Cuts at: 0 28 3321
Size: 28 3293
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 483 515 736 768 1427 1459 2029 2061
Size: 483 32 221 32 659 32 570 32
Cuts at: 2061 2479 2511 2868 2875 2900 2907 3321
Size: 418 32 357 7 25 7 414
Fragments arranged by size:
659 570 483 418 414 357 221 32
32 32 32 32 25 7 7
HincII GTy'rAC
Cuts at: 0 1551 3059 3321
Size: 1551 1508 262
Fragments arranged by size:
1551 1508 262
HindIII A'AGCT_T
Cuts at: 0 2253 3321
Size: 2253 1068
KasI G'GCGC_C
Cuts at: 0 24 3321
Size: 24 3297
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2205 3321
Size: 2205 1116
MscI TGG'CCA
Cuts at: 0 854 3321
Size: 854 2467
MslI CAynn'nnrTG
Cuts at: 0 321 787 967 1081 1300 1615 2033 2740
Size: 321 466 180 114 219 315 418 707
Cuts at: 2740 3321
Size: 581
Fragments arranged by size:
707 581 466 418 321 315 219 180
114
NarI GG'CG_CC
Cuts at: 0 25 3321
Size: 25 3296
NcoI C'CATG_G
Cuts at: 0 1784 3321
Size: 1784 1537
PflMI CCAn_nnn'nTGG
Cuts at: 0 1219 2551 2682 3321
Size: 1219 1332 131 639
Fragments arranged by size:
1332 1219 639 131
PfoI T'CCnGG_A
Cuts at: 0 41 3321
Size: 41 3280
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2546 2578 2907 2939 3321
Size: 2546 32 329 32 382
Fragments arranged by size:
2546 382 329 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 470 3321
Size: 470 2851
PshAI GACnn'nnGTC
Cuts at: 0 2426 3321
Size: 2426 895
PspOMI G'GGCC_C
Cuts at: 0 1239 3321
Size: 1239 2082
PstI C_TGCA'G
Cuts at: 0 1028 2943 3071 3321
Size: 1028 1915 128 250
Fragments arranged by size:
1915 1028 250 128
SacI G_AGCT'C
Cuts at: 0 2925 3321
Size: 2925 396
SanDI GG'GwC_CC
Cuts at: 0 470 3321
Size: 470 2851
ScaI AGT'ACT
Cuts at: 0 371 1056 3321
Size: 371 685 2265
Fragments arranged by size:
2265 685 371
SexAI A'CCwGG_T
Cuts at: 0 901 1540 2993 3321
Size: 901 639 1453 328
Fragments arranged by size:
1453 901 639 328
SfcI C'TryA_G
Cuts at: 0 1024 2939 3067 3321
Size: 1024 1915 128 254
Fragments arranged by size:
1915 1024 254 128
SfoI GGC'GCC
Cuts at: 0 26 3321
Size: 26 3295
SmlI C'TyrA_G
Cuts at: 0 1507 2250 3321
Size: 1507 743 1071
Fragments arranged by size:
1507 1071 743
SspI AAT'ATT
Cuts at: 0 1528 3321
Size: 1528 1793
StyI C'CwwG_G
Cuts at: 0 1232 1388 1784 2087 2431 3321
Size: 1232 156 396 303 344 890
Fragments arranged by size:
1232 890 396 344 303 156
TatI w'GTAC_w
Cuts at: 0 369 1054 1274 1427 3321
Size: 369 685 220 153 1894
Fragments arranged by size:
1894 685 369 220 153
Tth111I GACn'n_nGTC
Cuts at: 0 445 497 3321
Size: 445 52 2824
Fragments arranged by size:
2824 445 52
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 102 3321
Size: 102 3219
XmnI GAAnn'nnTTC
Cuts at: 0 1474 1529 1563 1991 2690 3321
Size: 1474 55 34 428 699 631
Fragments arranged by size:
1474 699 631 428 55 34
Enzymes that do cut:
AarI AccI AclI AcuI AflII AhdI AleI AlfI
AloI AlwNI ApaI ApaLI ApoI AvaI BaeI BamHI
BanI BanII BbeI BbsI BbvCI BclI BglI BglII
BlpI Bme1580I BplI BpmI Bpu10I BpuEI BsaI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI
EaeI EarI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
HaeII Hin4I HincII HindIII KasI MmeI MscI MslI
NarI NcoI PflMI PfoI PpiI PpuMI PshAI PspOMI
PstI SacI SanDI ScaI SexAI SfcI SfoI SmlI
SspI StyI TatI Tth111I XcmI XmnI
Enzymes that do not cut:
AatII Acc65I AfeI AflIII AgeI AscI AseI AsiSI
AvrII BcgI BciVI BfrBI BmgBI BmrI BmtI BsaAI
BsaBI BsiEI BsiWI BsmI BsrGI BssHII BstBI BstEII
BstZ17I ClaI DraIII DrdI EagI EciI EcoNI FseI
FspI FspAI HpaI KpnI MfeI MluI MspA1I NaeI
NdeI NgoMIV NheI NotI NruI NsiI NspI PacI
PasI PciI PmeI PmlI PsiI PspXI PsrI PvuI
PvuII RsrII SacII SalI SapI SbfI SfiI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI StuI SwaI
TaqII TstI XbaI XhoI XmaI ZraI