(Linear) (Six-base) MAPSORT of: KIEE3556.seq  Check: 9513  from: 1  to: 732



KIEE3556.orf

 With 182 enzymes: SgfI * 

                        July 3, 2012 18:10  ..

AccI GT'mk_AC

Cuts at:      0     634     732 
   Size:        634      98
 
ApoI r'AATT_y

Cuts at:      0     374     732 
   Size:        374     358
 
BanI G'GyrC_C

Cuts at:      0     567     732 
   Size:        567     165
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     181     732 
   Size:        181     551
 
Bme1580I G_kGCm'C

Cuts at:      0     572     732 
   Size:        572     160
 
BmrI ACTGGGnnnn_n'

Cuts at:      0       5      72     732 
   Size:          5      67     660

  Fragments arranged by size: 

                 660      67       5
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     175     732 
   Size:        175     557
 
BseYI C'CCAG_C

Cuts at:      0     571     732 
   Size:        571     161
 
Bsp1286I G_dGCh'C

Cuts at:      0     572     732 
   Size:        572     160
 
BssSI C'ACGA_G

Cuts at:      0      29     732 
   Size:         29     703
 
BstZ17I GTA'TAC

Cuts at:      0     635     732 
   Size:        635      97
 
BtgI C'CryG_G

Cuts at:      0     612     732 
   Size:        612     120
 
BtsI GCAGTG_nn'

Cuts at:      0     582     732 
   Size:        582     150
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     690     725     732 
   Size:        690      35       7

  Fragments arranged by size: 

                 690      35       7
 
EaeI y'GGCC_r

Cuts at:      0       2     250     732 
   Size:          2     248     482

  Fragments arranged by size: 

                 482     248       2
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     175     732 
   Size:        175     557
 
EcoO109I rG'GnC_Cy

Cuts at:      0     234     602     732 
   Size:        234     368     130

  Fragments arranged by size: 

                 368     234     130
 
EcoRI G'AATT_C

Cuts at:      0     374     732 
   Size:        374     358
 
EcoRV GAT'ATC

Cuts at:      0     258     732 
   Size:        258     474
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     276     308     732 
   Size:        276      32     424

  Fragments arranged by size: 

                 424     276      32
 
HindIII A'AGCT_T

Cuts at:      0     460     732 
   Size:        460     272
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     486     732 
   Size:        486     246
 
MspA1I CmG'CkG

Cuts at:      0     445     732 
   Size:        445     287
 
NcoI C'CATG_G

Cuts at:      0     612     732 
   Size:        612     120
 
NdeI CA'TA_TG

Cuts at:      0     180     732 
   Size:        180     552
 
PasI CC'CwG_GG

Cuts at:      0     238     732 
   Size:        238     494
 
PpuMI rG'GwC_Cy

Cuts at:      0     234     732 
   Size:        234     498
 
PvuII CAG'CTG

Cuts at:      0     445     732 
   Size:        445     287
 
SanDI GG'GwC_CC

Cuts at:      0     234     732 
   Size:        234     498
 
SfcI C'TryA_G

Cuts at:      0     316     732 
   Size:        316     416
 
StyI C'CwwG_G

Cuts at:      0     612     732 
   Size:        612     120
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      93     277     732 
   Size:         93     184     455

  Fragments arranged by size: 

                 455     184      93
 

 Enzymes that do cut:

     AccI     ApoI     BanI     BbsI Bme1580I     BmrI     BpmI    BseYI
 Bsp1286I    BssSI  BstZ17I     BtgI     BtsI    CspCI     EaeI  Eco57MI
 EcoO109I    EcoRI    EcoRV    Hin4I  HindIII     MmeI   MspA1I     NcoI
     NdeI     PasI    PpuMI    PvuII    SanDI     SfcI     StyI    TaqII

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII
     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI    BmgBI     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI     BsgI    BsiEI  BsiHKAI
    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII   BstAPI    BstBI   BstEII    BstXI    BstYI
   Bsu36I    BtgZI     ClaI     DraI   DraIII     DrdI     EagI     EarI
     EciI  EcoICRI    EcoNI     FalI     FseI     FspI    FspAI    HaeII
   HincII     HpaI     KasI     KpnI     MfeI     MluI     MscI     MslI
     NaeI     NarI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII     SacI
    SacII     SalI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     SwaI     TatI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI