Restriction map for
pFN21AE3555
(Linear) (Six-base) MAPSORT of: KIEE3555.seq Check: 3347 from: 1 to: 15621
KIEE3555.orf
With 182 enzymes: SgfI *
April 17, 2013 10:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 4052 5971 6400 9917 14192 15621
Size: 4052 1919 429 3517 4275 1429
Fragments arranged by size:
4275 4052 3517 1919 1429 429
AatII G_ACGT'C
Cuts at: 0 6313 7034 9467 9650 15621
Size: 6313 721 2433 183 5971
Fragments arranged by size:
6313 5971 2433 721 183
AccI GT'mk_AC
Cuts at: 0 5540 5672 7088 7694 12209 14684 15145 15621
Size: 5540 132 1416 606 4515 2475 461 476
Fragments arranged by size:
5540 4515 2475 1416 606 476 461 132
Acc65I G'GTAC_C
Cuts at: 0 1794 5261 7318 12977 15621
Size: 1794 3467 2057 5659 2644
Fragments arranged by size:
5659 3467 2644 2057 1794
AclI AA'CG_TT
Cuts at: 0 2447 15621
Size: 2447 13174
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 184 722 992 1028 1596 2206 2759 3025
Size: 184 538 270 36 568 610 553 266
Cuts at: 3025 3165 3502 4098 4342 4437 4576 5811 6317
Size: 140 337 596 244 95 139 1235 506
Cuts at: 6317 8302 8416 8590 8943 9883 11275 11644 12512
Size: 1985 114 174 353 940 1392 369 868
Cuts at: 12512 13090 13472 13882 15621
Size: 578 382 410 1739
Fragments arranged by size:
1985 1739 1392 1235 940 868 610 596
578 568 553 538 506 410 382 369
353 337 270 266 244 184 174 140
139 114 95 36
AfeI AGC'GCT
Cuts at: 0 6338 7927 15451 15621
Size: 6338 1589 7524 170
Fragments arranged by size:
7524 6338 1589 170
AflII C'TTAA_G
Cuts at: 0 2584 3961 5223 12306 12613 15621
Size: 2584 1377 1262 7083 307 3008
Fragments arranged by size:
7083 3008 2584 1377 1262 307
AflIII A'CryG_T
Cuts at: 0 1788 6346 6348 6542 10715 13705 15185 15621
Size: 1788 4558 2 194 4173 2990 1480 436
Fragments arranged by size:
4558 4173 2990 1788 1480 436 194 2
AgeI A'CCGG_T
Cuts at: 0 661 15621
Size: 661 14960
AhdI GACnn_n'nnGTC
Cuts at: 0 2540 12216 15621
Size: 2540 9676 3405
Fragments arranged by size:
9676 3405 2540
AleI CACnn'nnGTG
Cuts at: 0 1722 4813 9986 10038 14935 15621
Size: 1722 3091 5173 52 4897 686
Fragments arranged by size:
5173 4897 3091 1722 686 52
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2144 4267 5043 8003 8951 9122 11610 11620
Size: 2144 2123 776 2960 948 171 2488 10
Cuts at: 11620 15621
Size: 4001
Fragments arranged by size:
4001 2960 2488 2144 2123 948 776 171
10
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 97 129 15621
Size: 97 32 15492
Fragments arranged by size:
15492 97 32
AlwNI CAG_nnn'CTG
Cuts at: 0 100 162 350 1051 4260 4512 5565 5919
Size: 100 62 188 701 3209 252 1053 354
Cuts at: 5919 6454 7059 7533 7800 9162 11838 12068 12945
Size: 535 605 474 267 1362 2676 230 877
Cuts at: 12945 13800 15243 15270 15592 15621
Size: 855 1443 27 322 29
Fragments arranged by size:
3209 2676 1443 1362 1053 877 855 701
605 535 474 354 322 267 252 230
188 100 62 29 27
ApaI G_GGCC'C
Cuts at: 0 181 8365 8664 15621
Size: 181 8184 299 6957
Fragments arranged by size:
8184 6957 299 181
ApaLI G'TGCA_C
Cuts at: 0 3972 5745 7351 11440 12043 15621
Size: 3972 1773 1606 4089 603 3578
Fragments arranged by size:
4089 3972 3578 1773 1606 603
ApoI r'AATT_y
Cuts at: 0 1219 3070 4137 6194 7291 8845 9992 12124
Size: 1219 1851 1067 2057 1097 1554 1147 2132
Cuts at: 12124 13204 13986 15515 15621
Size: 1080 782 1529 106
Fragments arranged by size:
2132 2057 1851 1554 1529 1219 1147 1097
1080 1067 782 106
AvaI C'yCGr_G
Cuts at: 0 192 220 450 757 1122 2506 5160 5463
Size: 192 28 230 307 365 1384 2654 303
Cuts at: 5463 5866 6819 7716 9010 9716 10262 11413 11824
Size: 403 953 897 1294 706 546 1151 411
Cuts at: 11824 13036 13616 15621
Size: 1212 580 2005
Fragments arranged by size:
2654 2005 1384 1294 1212 1151 953 897
706 580 546 411 403 365 307 303
230 192 28
AvrII C'CTAG_G
Cuts at: 0 3112 11802 15621
Size: 3112 8690 3819
Fragments arranged by size:
8690 3819 3112
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 6335 6368 13497 13530 15621
Size: 6335 33 7129 33 2091
Fragments arranged by size:
7129 6335 2091 33 33
BamHI G'GATC_C
Cuts at: 0 7145 7238 12692 13830 15120 15621
Size: 7145 93 5454 1138 1290 501
Fragments arranged by size:
7145 5454 1290 1138 501 93
BanI G'GyrC_C
Cuts at: 0 1794 2077 2779 4647 5261 5360 5859 6109
Size: 1794 283 702 1868 614 99 499 250
Cuts at: 6109 6574 7074 7318 9084 12821 12977 15621
Size: 465 500 244 1766 3737 156 2644
Fragments arranged by size:
3737 2644 1868 1794 1766 702 614 500
499 465 283 250 244 156 99
BanII G_rGCy'C
Cuts at: 0 181 199 230 722 758 1123 1465 3013
Size: 181 18 31 492 36 365 342 1548
Cuts at: 3013 3643 3779 4199 4711 5147 5291 5656 6331
Size: 630 136 420 512 436 144 365 675
Cuts at: 6331 7184 7672 8365 8664 8729 9572 10023 11684
Size: 853 488 693 299 65 843 451 1661
Cuts at: 11684 11754 13391 15331 15621
Size: 70 1637 1940 290
Fragments arranged by size:
1940 1661 1637 1548 853 843 693 675
630 512 492 488 451 436 420 365
365 342 299 290 181 144 136 70
65 36 31 18
BbeI G_GCGC'C
Cuts at: 0 2081 6113 15621
Size: 2081 4032 9508
Fragments arranged by size:
9508 4032 2081
BbsI GAAGACnn'nnnn_
Cuts at: 0 880 1016 1191 2697 3171 4564 4827 5771
Size: 880 136 175 1506 474 1393 263 944
Cuts at: 5771 5835 8247 8555 8578 8600 8615 9642 10451
Size: 64 2412 308 23 22 15 1027 809
Cuts at: 10451 11847 11866 11902 12559 13460 13725 15621
Size: 1396 19 36 657 901 265 1896
Fragments arranged by size:
2412 1896 1506 1396 1393 1027 944 901
880 809 657 474 308 265 263 175
136 64 36 23 22 19 15
BbvCI CC'TCA_GC
Cuts at: 0 798 3556 5332 7538 10176 11965 14332 14437
Size: 798 2758 1776 2206 2638 1789 2367 105
Cuts at: 14437 15349 15621
Size: 912 272
Fragments arranged by size:
2758 2638 2367 2206 1789 1776 912 798
272 105
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 946 980 2326 2360 5454 5488 12697 12731
Size: 946 34 1346 34 3094 34 7209 34
Cuts at: 12731 15621
Size: 2890
Fragments arranged by size:
7209 3094 2890 1346 946 34 34 34
34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1994 3046 10752 12811 15621
Size: 1994 1052 7706 2059 2810
Fragments arranged by size:
7706 2810 2059 1994 1052
BclI T'GATC_A
Cuts at: 0 3095 7509 7841 9050 15171 15621
Size: 3095 4414 332 1209 6121 450
Fragments arranged by size:
6121 4414 3095 1209 450 332
BfrBI ATG'CAT
Cuts at: 0 2048 2993 8603 15621
Size: 2048 945 5610 7018
Fragments arranged by size:
7018 5610 2048 945
BglI GCCn_nnn'nGGC
Cuts at: 0 431 808 5533 5887 11590 12658 13423 14621
Size: 431 377 4725 354 5703 1068 765 1198
Cuts at: 14621 15621
Size: 1000
Fragments arranged by size:
5703 4725 1198 1068 1000 765 431 377
354
BglII A'GATC_T
Cuts at: 0 6861 8379 9272 10986 15621
Size: 6861 1518 893 1714 4635
Fragments arranged by size:
6861 4635 1714 1518 893
BlpI GC'TnA_GC
Cuts at: 0 827 1117 2251 3012 4855 5698 9808 12847
Size: 827 290 1134 761 1843 843 4110 3039
Cuts at: 12847 14911 15341 15621
Size: 2064 430 280
Fragments arranged by size:
4110 3039 2064 1843 1134 843 827 761
430 290 280
Bme1580I G_kGCm'C
Cuts at: 0 181 457 3976 5666 5749 6435 7079 7355
Size: 181 276 3519 1690 83 686 644 276
Cuts at: 7355 8184 8365 8664 10855 11444 12047 14297 15621
Size: 829 181 299 2191 589 603 2250 1324
Fragments arranged by size:
3519 2250 2191 1690 1324 829 686 644
603 589 299 276 276 181 181 83
BmgBI CAC'GTC
Cuts at: 0 3177 4907 6171 7920 11853 11892 13698 15621
Size: 3177 1730 1264 1749 3933 39 1806 1923
Fragments arranged by size:
3933 3177 1923 1806 1749 1730 1264 39
BmrI ACTGGGnnnn_n'
Cuts at: 0 1656 1837 5808 6351 7019 8667 11624 11748
Size: 1656 181 3971 543 668 1648 2957 124
Cuts at: 11748 13060 13720 14037 14158 15621
Size: 1312 660 317 121 1463
Fragments arranged by size:
3971 2957 1656 1648 1463 1312 668 660
543 317 181 124 121
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 92 2190 14452 15497 15499 15621
Size: 92 2098 12262 1045 2 122
Fragments arranged by size:
12262 2098 1045 122 92 2
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 493 1504 2154 2185 2835 3337 4204 6577
Size: 493 1011 650 31 650 502 867 2373
Cuts at: 6577 7051 7368 9526 9559 9946 9948 10822 10825
Size: 474 317 2158 33 387 2 874 3
Cuts at: 10825 11176 11698 12116 12552 12553 12611 13510 13805
Size: 351 522 418 436 1 58 899 295
Cuts at: 13805 13891 14086 14239 14878 15621
Size: 86 195 153 639 743
Fragments arranged by size:
2373 2158 1011 899 874 867 743 650
650 639 522 502 493 474 436 418
387 351 317 295 195 153 86 58
33 31 3 2 1
Bpu10I CC'TnA_GC
Cuts at: 0 798 1464 1984 3556 3637 5134 5146 5332
Size: 798 666 520 1572 81 1497 12 186
Cuts at: 5332 7297 7538 10176 10418 11965 14332 14437 15349
Size: 1965 241 2638 242 1547 2367 105 912
Cuts at: 15349 15621
Size: 272
Fragments arranged by size:
2638 2367 1965 1572 1547 1497 912 798
666 520 272 242 241 186 105 81
12
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1000 2425 3826 7141 12568 13451 14548 14902
Size: 1000 1425 1401 3315 5427 883 1097 354
Cuts at: 14902 15134 15621
Size: 232 487
Fragments arranged by size:
5427 3315 1425 1401 1097 1000 883 487
354 232
BsaI GGTCTCn'nnnn_
Cuts at: 0 934 5392 5447 10637 13903 14830 15621
Size: 934 4458 55 5190 3266 927 791
Fragments arranged by size:
5190 4458 3266 934 927 791 55
BsaAI yAC'GTr
Cuts at: 0 1791 6003 6349 8704 9147 9384 11796 12042
Size: 1791 4212 346 2355 443 237 2412 246
Cuts at: 12042 15186 15621
Size: 3144 435
Fragments arranged by size:
4212 3144 2412 2355 1791 443 435 346
246 237
BsaBI GATnn'nnATC
Cuts at: 0 13807 15621
Size: 13807 1814
BsaHI Gr'CG_yC
Cuts at: 0 124 2078 6110 6310 7031 8351 9464 9647
Size: 124 1954 4032 200 721 1320 1113 183
Cuts at: 9647 9671 11401 15380 15621
Size: 24 1730 3979 241
Fragments arranged by size:
4032 3979 1954 1730 1320 1113 721 241
200 183 124 24
BsaWI w'CCGG_w
Cuts at: 0 661 2273 6881 7229 7580 10334 15621
Size: 661 1612 4608 348 351 2754 5287
Fragments arranged by size:
5287 4608 2754 1612 661 351 348
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 97 127 472 502 709 739 894 924
Size: 97 30 345 30 207 30 155 30
Cuts at: 924 1124 1154 2503 2533 3424 3454 3625 3655
Size: 200 30 1349 30 891 30 171 30
Cuts at: 3655 4327 4357 6160 6190 6782 6812 9832 9862
Size: 672 30 1803 30 592 30 3020 30
Cuts at: 9862 10019 10049 11149 11179 12754 12784 15621
Size: 157 30 1100 30 1575 30 2837
Fragments arranged by size:
3020 2837 1803 1575 1349 1100 891 672
592 345 207 200 171 157 155 97
30 30 30 30 30 30 30 30
30 30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 238 899 1145 1223 1226 1863 2524 3594
Size: 238 661 246 78 3 637 661 1070
Cuts at: 3594 4738 4762 5286 5629 5740 6168 6208 6339
Size: 1144 24 524 343 111 428 40 131
Cuts at: 6339 6453 7609 7663 8745 9580 9769 10471 10525
Size: 114 1156 54 1082 835 189 702 54
Cuts at: 10525 10678 11173 11228 11356 12274 14532 14839 15337
Size: 153 495 55 128 918 2258 307 498
Cuts at: 15337 15460 15621
Size: 123 161
Fragments arranged by size:
2258 1156 1144 1082 1070 918 835 702
661 661 637 524 498 495 428 343
307 246 238 189 161 153 131 128
123 114 111 78 55 54 54 40
24 3
BseYI C'CCAG_C
Cuts at: 0 413 2073 2871 2942 3661 4286 4441 4885
Size: 413 1660 798 71 719 625 155 444
Cuts at: 4885 7665 9242 9822 10163 10422 11258 13413 13674
Size: 2780 1577 580 341 259 836 2155 261
Cuts at: 13674 13890 15010 15621
Size: 216 1120 611
Fragments arranged by size:
2780 2155 1660 1577 1120 836 798 719
625 611 580 444 413 341 261 259
216 155 71
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1064 1243 2077 4030 4426 4672 4861 4867
Size: 1064 179 834 1953 396 246 189 6
Cuts at: 4867 5190 5577 6205 7917 8445 8563 9895 10639
Size: 323 387 628 1712 528 118 1332 744
Cuts at: 10639 10644 11104 11364 11580 12895 14863 15262 15382
Size: 5 460 260 216 1315 1968 399 120
Cuts at: 15382 15621
Size: 239
Fragments arranged by size:
1968 1953 1712 1332 1315 1064 834 744
628 528 460 399 396 387 323 260
246 239 216 189 179 120 118 6
5
BsiEI CG_ry'CG
Cuts at: 0 7658 12897 15621
Size: 7658 5239 2724
Fragments arranged by size:
7658 5239 2724
BsiHKAI G_wGCw'C
Cuts at: 0 722 2339 3013 3643 3976 4199 5045 5093
Size: 722 1617 674 630 333 223 846 48
Cuts at: 5093 5140 5147 5291 5749 5807 6158 6331 7184
Size: 47 7 144 458 58 351 173 853
Cuts at: 7184 7355 7961 8729 9025 9572 10688 11444 11684
Size: 171 606 768 296 547 1116 756 240
Cuts at: 11684 11702 11795 11801 11852 11992 12047 15109 15621
Size: 18 93 6 51 140 55 3062 512
Fragments arranged by size:
3062 1617 1116 853 846 768 756 722
674 630 606 547 512 458 351 333
296 240 223 173 171 144 140 93
58 55 51 48 47 18 7 6
BsmI GAATG_Cn'
Cuts at: 0 4459 6592 7992 11944 12481 13222 15621
Size: 4459 2133 1400 3952 537 741 2399
Fragments arranged by size:
4459 3952 2399 2133 1400 741 537
BsmBI CGTCTCn'nnnn_
Cuts at: 0 952 7022 7645 8517 10928 13688 14524 15621
Size: 952 6070 623 872 2411 2760 836 1097
Fragments arranged by size:
6070 2760 2411 1097 952 872 836 623
Bsp1286I G_dGCh'C
Cuts at: 0 181 199 230 457 722 758 1123 1465
Size: 181 18 31 227 265 36 365 342
Cuts at: 1465 2339 3013 3643 3779 3976 4199 4711 5045
Size: 874 674 630 136 197 223 512 334
Cuts at: 5045 5093 5140 5147 5291 5656 5666 5749 5807
Size: 48 47 7 144 365 10 83 58
Cuts at: 5807 6158 6331 6435 7079 7184 7355 7672 7961
Size: 351 173 104 644 105 171 317 289
Cuts at: 7961 8184 8365 8664 8729 9025 9572 10023 10688
Size: 223 181 299 65 296 547 451 665
Cuts at: 10688 10855 11444 11684 11702 11754 11795 11801 11852
Size: 167 589 240 18 52 41 6 51
Cuts at: 11852 11992 12047 13391 14297 15109 15331 15621
Size: 140 55 1344 906 812 222 290
Fragments arranged by size:
1344 906 874 812 674 665 644 630
589 547 512 451 365 365 351 342
334 317 299 296 290 289 265 240
227 223 223 222 197 181 181 173
171 167 144 140 136 105 104 83
65 58 55 52 51 48 47 41
36 31 18 18 10 7 6
BspEI T'CCGG_A
Cuts at: 0 6881 10334 15621
Size: 6881 3453 5287
Fragments arranged by size:
6881 5287 3453
BspHI T'CATG_A
Cuts at: 0 1281 1529 4463 6761 7034 7874 8258 12429
Size: 1281 248 2934 2298 273 840 384 4171
Cuts at: 12429 15621
Size: 3192
Fragments arranged by size:
4171 3192 2934 2298 1281 840 384 273
248
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 4052 4071 4520 4883 5466 5881 5971 6400
Size: 4052 19 449 363 583 415 90 429
Cuts at: 6400 7313 8391 8507 9320 9684 9917 11237 11960
Size: 913 1078 116 813 364 233 1320 723
Cuts at: 11960 12683 12785 14192 15621
Size: 723 102 1407 1429
Fragments arranged by size:
4052 1429 1407 1320 1078 913 813 723
723 583 449 429 415 364 363 233
116 102 90 19
BsrBI CCG'CTC
Cuts at: 0 2161 12392 14644 14778 15621
Size: 2161 10231 2252 134 843
Fragments arranged by size:
10231 2252 2161 843 134
BsrDI GCAATG_nn'
Cuts at: 0 1798 8933 10169 11594 13192 14205 15224 15621
Size: 1798 7135 1236 1425 1598 1013 1019 397
Fragments arranged by size:
7135 1798 1598 1425 1236 1019 1013 397
BsrFI r'CCGG_y
Cuts at: 0 661 2390 5945 9500 13663 15621
Size: 661 1729 3555 3555 4163 1958
Fragments arranged by size:
4163 3555 3555 1958 1729 661
BsrGI T'GTAC_A
Cuts at: 0 9470 12509 13145 14939 15621
Size: 9470 3039 636 1794 682
Fragments arranged by size:
9470 3039 1794 682 636
BssSI C'ACGA_G
Cuts at: 0 4720 4730 7954 9127 13264 15621
Size: 4720 10 3224 1173 4137 2357
Fragments arranged by size:
4720 4137 3224 2357 1173 10
BstAPI GCAn_nnn'nTGC
Cuts at: 0 59 817 1310 2247 2939 8586 9162 12051
Size: 59 758 493 937 692 5647 576 2889
Cuts at: 12051 12945 13580 14313 15621
Size: 894 635 733 1308
Fragments arranged by size:
5647 2889 1308 937 894 758 733 692
635 576 493 59
BstEII G'GTnAC_C
Cuts at: 0 3280 3891 6753 6834 11841 14158 15621
Size: 3280 611 2862 81 5007 2317 1463
Fragments arranged by size:
5007 3280 2862 2317 1463 611 81
BstXI CCAn_nnnn'nTGG
Cuts at: 0 421 471 1275 1996 4545 7341 8235 10350
Size: 421 50 804 721 2549 2796 894 2115
Cuts at: 10350 11266 11676 12000 13890 13920 15621
Size: 916 410 324 1890 30 1701
Fragments arranged by size:
2796 2549 2115 1890 1701 916 894 804
721 421 410 324 50 30
BstYI r'GATC_y
Cuts at: 0 3873 6462 6861 7145 7238 8034 8187 8379
Size: 3873 2589 399 284 93 796 153 192
Cuts at: 8379 9158 9272 10339 10986 12692 13150 13223 13830
Size: 779 114 1067 647 1706 458 73 607
Cuts at: 13830 14376 14580 14600 15120 15321 15621
Size: 546 204 20 520 201 300
Fragments arranged by size:
3873 2589 1706 1067 796 779 647 607
546 520 458 399 300 284 204 201
192 153 114 93 73 20
BstZ17I GTA'TAC
Cuts at: 0 7695 15146 15621
Size: 7695 7451 475
Fragments arranged by size:
7695 7451 475
Bsu36I CC'TnA_GG
Cuts at: 0 383 2144 3740 5212 6083 6436 7140 7912
Size: 383 1761 1596 1472 871 353 704 772
Cuts at: 7912 8950 11507 12061 12737 15621
Size: 1038 2557 554 676 2884
Fragments arranged by size:
2884 2557 1761 1596 1472 1038 871 772
704 676 554 383 353
BtgI C'CryG_G
Cuts at: 0 376 2116 2843 3119 3977 4610 4916 5447
Size: 376 1740 727 276 858 633 306 531
Cuts at: 5447 6839 9797 10296 10517 10628 11265 11434 13421
Size: 1392 2958 499 221 111 637 169 1987
Cuts at: 13421 13535 14690 14726 15621
Size: 114 1155 36 895
Fragments arranged by size:
2958 1987 1740 1392 1155 895 858 727
637 633 531 499 376 306 276 221
169 114 111 36
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 7981 8103 8208 10477 13007 13244 15071 15621
Size: 7981 122 105 2269 2530 237 1827 550
Fragments arranged by size:
7981 2530 2269 1827 550 237 122 105
BtsI GCAGTG_nn'
Cuts at: 0 924 2094 2921 4848 5805 6609 6990 8476
Size: 924 1170 827 1927 957 804 381 1486
Cuts at: 8476 9151 10578 12045 12775 15621
Size: 675 1427 1467 730 2846
Fragments arranged by size:
2846 1927 1486 1467 1427 1170 957 924
827 804 730 675 381
ClaI AT'CG_AT
Cuts at: 0 9766 10325 15621
Size: 9766 559 5296
Fragments arranged by size:
9766 5296 559
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 486 521 15621
Size: 486 35 15100
Fragments arranged by size:
15100 486 35
DraI TTT'AAA
Cuts at: 0 3143 10769 13985 15621
Size: 3143 7626 3216 1636
Fragments arranged by size:
7626 3216 3143 1636
DraIII CAC_nnn'GTG
Cuts at: 0 8412 12828 14265 15621
Size: 8412 4416 1437 1356
Fragments arranged by size:
8412 4416 1437 1356
DrdI GACnn_nn'nnGTC
Cuts at: 0 8767 15621
Size: 8767 6854
EaeI y'GGCC_r
Cuts at: 0 432 551 2972 4607 4613 4659 5406 5481
Size: 432 119 2421 1635 6 46 747 75
Cuts at: 5481 5505 7524 7655 8060 8999 9372 10449 12755
Size: 24 2019 131 405 939 373 1077 2306
Cuts at: 12755 12894 13022 13337 13521 13665 13880 14141 15621
Size: 139 128 315 184 144 215 261 1480
Fragments arranged by size:
2421 2306 2019 1635 1480 1077 939 747
432 405 373 315 261 215 184 144
139 131 128 119 75 46 24 6
EagI C'GGCC_G
Cuts at: 0 7655 12894 15621
Size: 7655 5239 2727
Fragments arranged by size:
7655 5239 2727
EarI CTCTTCn'nnn_
Cuts at: 0 1169 1753 2275 3709 5056 5279 6001 6336
Size: 1169 584 522 1434 1347 223 722 335
Cuts at: 6336 6703 6880 8299 8389 9449 9547 9616 10266
Size: 367 177 1419 90 1060 98 69 650
Cuts at: 10266 10775 11414 11788 12292 12523 12590 15542 15621
Size: 509 639 374 504 231 67 2952 79
Fragments arranged by size:
2952 1434 1419 1347 1169 1060 722 650
639 584 522 509 504 374 367 335
231 223 177 98 90 79 69 67
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 4524 8560 9427 10456 10961 12236 12685 15445
Size: 4524 4036 867 1029 505 1275 449 2760
Cuts at: 15445 15621
Size: 176
Fragments arranged by size:
4524 4036 2760 1275 1029 867 505 449
176
EcoICRI GAG'CTC
Cuts at: 0 720 3011 3641 4197 5145 5289 6329 7182
Size: 720 2291 630 556 948 144 1040 853
Cuts at: 7182 8727 9570 11682 15621
Size: 1545 843 2112 3939
Fragments arranged by size:
3939 2291 2112 1545 1040 948 853 843
720 630 556 144
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 184 493 722 992 1028 1504 1596 2154
Size: 184 309 229 270 36 476 92 558
Cuts at: 2154 2185 2206 2759 2835 3025 3165 3337 3502
Size: 31 21 553 76 190 140 172 165
Cuts at: 3502 4098 4204 4342 4437 4576 5811 6317 6577
Size: 596 106 138 95 139 1235 506 260
Cuts at: 6577 7051 7368 8302 8416 8590 8943 9526 9559
Size: 474 317 934 114 174 353 583 33
Cuts at: 9559 9883 9946 9948 10822 10825 11176 11275 11644
Size: 324 63 2 874 3 351 99 369
Cuts at: 11644 11698 12116 12512 12552 12553 12611 13090 13472
Size: 54 418 396 40 1 58 479 382
Cuts at: 13472 13510 13805 13882 13891 14086 14239 14878 15621
Size: 38 295 77 9 195 153 639 743
Fragments arranged by size:
1235 934 874 743 639 596 583 558
553 506 479 476 474 418 396 382
369 353 351 324 317 309 295 270
260 229 195 190 184 174 172 165
153 140 139 138 114 106 99 95
92 77 76 63 58 54 40 38
36 33 31 21 9 3 2 1
EcoNI CCTnn'n_nnAGG
Cuts at: 0 649 1567 3111 4048 4270 7051 8398 8770
Size: 649 918 1544 937 222 2781 1347 372
Cuts at: 8770 11382 11725 12595 12679 12740 14317 14370 15621
Size: 2612 343 870 84 61 1577 53 1251
Fragments arranged by size:
2781 2612 1577 1544 1347 1251 937 918
870 649 372 343 222 84 61 53
EcoO109I rG'GnC_Cy
Cuts at: 0 177 794 1560 2714 5228 5904 6147 7044
Size: 177 617 766 1154 2514 676 243 897
Cuts at: 7044 7056 8361 8496 8661 9401 9712 12015 12065
Size: 12 1305 135 165 740 311 2303 50
Cuts at: 12065 12879 13297 13866 14622 14781 15621
Size: 814 418 569 756 159 840
Fragments arranged by size:
2514 2303 1305 1154 897 840 814 766
756 740 676 617 569 418 311 243
177 165 159 135 50 12
EcoRI G'AATT_C
Cuts at: 0 8845 12124 15621
Size: 8845 3279 3497
Fragments arranged by size:
8845 3497 3279
EcoRV GAT'ATC
Cuts at: 0 9525 10919 13242 15621
Size: 9525 1394 2323 2379
Fragments arranged by size:
9525 2379 2323 1394
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2734 3195 3690 4092 8655 8865 12321 12441
Size: 2734 461 495 402 4563 210 3456 120
Cuts at: 12441 15621
Size: 3180
Fragments arranged by size:
4563 3456 3180 2734 495 461 402 210
120
FspI TGC'GCA
Cuts at: 0 6134 11926 13939 15621
Size: 6134 5792 2013 1682
Fragments arranged by size:
6134 5792 2013 1682
FspAI rTGC'GCAy
Cuts at: 0 11926 15621
Size: 11926 3695
HaeII r_GCGC'y
Cuts at: 0 2081 6113 6340 6871 7929 8741 8835 9815
Size: 2081 4032 227 531 1058 812 94 980
Cuts at: 9815 9933 10163 10628 11413 12306 15453 15621
Size: 118 230 465 785 893 3147 168
Fragments arranged by size:
4032 3147 2081 1058 980 893 812 785
531 465 230 227 168 118 94
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 472 504 1942 1974 2503 2526 2535 2558
Size: 472 32 1438 32 529 23 9 23
Cuts at: 2558 3865 3897 3989 4021 4327 4359 4864 4896
Size: 1307 32 92 32 306 32 505 32
Cuts at: 4896 6066 6098 6160 6192 8024 8056 9097 9129
Size: 1170 32 62 32 1832 32 1041 32
Cuts at: 9129 9696 9728 9748 9780 9832 9864 10331 10363
Size: 567 32 20 32 52 32 467 32
Cuts at: 10363 11913 11945 12112 12144 12202 12234 12712 12744
Size: 1550 32 167 32 58 32 478 32
Cuts at: 12744 14282 14314 15621
Size: 1538 32 1307
Fragments arranged by size:
1832 1550 1538 1438 1307 1307 1170 1041
567 529 505 478 472 467 306 167
92 62 58 52 32 32 32 32
32 32 32 32 32 32 32 32
32 32 32 32 32 32 32 23
23 20 9
HincII GTy'rAC
Cuts at: 0 1428 3266 7089 11336 13118 14006 15621
Size: 1428 1838 3823 4247 1782 888 1615
Fragments arranged by size:
4247 3823 1838 1782 1615 1428 888
HindIII A'AGCT_T
Cuts at: 0 8981 15621
Size: 8981 6640
HpaI GTT'AAC
Cuts at: 0 13118 15621
Size: 13118 2503
KasI G'GCGC_C
Cuts at: 0 2077 6109 15621
Size: 2077 4032 9512
Fragments arranged by size:
9512 4032 2077
KpnI G_GTAC'C
Cuts at: 0 1798 5265 7322 12981 15621
Size: 1798 3467 2057 5659 2640
Fragments arranged by size:
5659 3467 2640 2057 1798
MluI A'CGCG_T
Cuts at: 0 10715 15621
Size: 10715 4906
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 3288 5967 6878 7418 8678 11141 11518 11743
Size: 3288 2679 911 540 1260 2463 377 225
Cuts at: 11743 13465 14828 15621
Size: 1722 1363 793
Fragments arranged by size:
3288 2679 2463 1722 1363 1260 911 793
540 377 225
MscI TGG'CCA
Cuts at: 0 434 553 2974 4609 4661 5408 5507 7526
Size: 434 119 2421 1635 52 747 99 2019
Cuts at: 7526 8062 9001 12757 13024 13339 13882 14143 15621
Size: 536 939 3756 267 315 543 261 1478
Fragments arranged by size:
3756 2421 2019 1635 1478 939 747 543
536 434 315 267 261 119 99 52
MslI CAynn'nnrTG
Cuts at: 0 1722 2126 3586 4372 4636 4813 6940 7219
Size: 1722 404 1460 786 264 177 2127 279
Cuts at: 7219 8068 8695 8881 9986 10038 10325 10348 11060
Size: 849 627 186 1105 52 287 23 712
Cuts at: 11060 11080 11329 11602 13918 14935 15621
Size: 20 249 273 2316 1017 686
Fragments arranged by size:
2316 2127 1722 1460 1105 1017 849 786
712 686 627 404 287 279 273 264
249 186 177 52 23 20
MspA1I CmG'CkG
Cuts at: 0 1048 1433 1588 1722 2118 2845 3121 3729
Size: 1048 385 155 134 396 727 276 608
Cuts at: 3729 4354 5057 5124 5295 5639 5697 7621 7689
Size: 625 703 67 171 344 58 1924 68
Cuts at: 7689 7983 8586 9048 9838 10395 11135 11676 11714
Size: 294 603 462 790 557 740 541 38
Cuts at: 11714 12136 12858 13689 14047 14469 14871 15042 15112
Size: 422 722 831 358 422 402 171 70
Cuts at: 15112 15135 15267 15566 15589 15621
Size: 23 132 299 23 32
Fragments arranged by size:
1924 1048 831 790 740 727 722 703
625 608 603 557 541 462 422 422
402 396 385 358 344 299 294 276
171 171 155 134 132 70 68 67
58 38 32 23 23
NarI GG'CG_CC
Cuts at: 0 2078 6110 15621
Size: 2078 4032 9511
Fragments arranged by size:
9511 4032 2078
NcoI C'CATG_G
Cuts at: 0 376 4916 6839 10517 11265 13421 15621
Size: 376 4540 1923 3678 748 2156 2200
Fragments arranged by size:
4540 3678 2200 2156 1923 748 376
NdeI CA'TA_TG
Cuts at: 0 7514 15621
Size: 7514 8107
NsiI A_TGCA'T
Cuts at: 0 2050 2995 8605 15621
Size: 2050 945 5610 7016
Fragments arranged by size:
7016 5610 2050 945
NspI r_CATG'y
Cuts at: 0 25 2766 5547 10381 10638 11706 12175 12516
Size: 25 2741 2781 4834 257 1068 469 341
Cuts at: 12516 13432 13709 14758 15132 15498 15621
Size: 916 277 1049 374 366 123
Fragments arranged by size:
4834 2781 2741 1068 1049 916 469 374
366 341 277 257 123 25
PasI CC'CwG_GG
Cuts at: 0 5649 5658 10976 15621
Size: 5649 9 5318 4645
Fragments arranged by size:
5649 5318 4645 9
PciI A'CATG_T
Cuts at: 0 13705 15621
Size: 13705 1916
PflMI CCAn_nnn'nTGG
Cuts at: 0 534 3657 5415 7533 7800 8985 11550 13674
Size: 534 3123 1758 2118 267 1185 2565 2124
Cuts at: 13674 14303 15621
Size: 629 1318
Fragments arranged by size:
3123 2565 2124 2118 1758 1318 1185 629
534 267
PfoI T'CCnGG_A
Cuts at: 0 244 587 1094 2189 2363 2397 2480 4220
Size: 244 343 507 1095 174 34 83 1740
Cuts at: 4220 4292 6125 6296 8771 10586 10841 15621
Size: 72 1833 171 2475 1815 255 4780
Fragments arranged by size:
4780 2475 1833 1815 1740 1095 507 343
255 244 174 171 83 72 34
PmlI CAC'GTG
Cuts at: 0 1791 6349 8704 11796 12042 15186 15621
Size: 1791 4558 2355 3092 246 3144 435
Fragments arranged by size:
4558 3144 3092 2355 1791 435 246
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 97 129 1372 1404 7210 7242 15621
Size: 97 32 1243 32 5806 32 8379
Fragments arranged by size:
8379 5806 1243 97 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 1560 2714 5904 6147 7044 8496 9401 9712
Size: 1560 1154 3190 243 897 1452 905 311
Cuts at: 9712 12015 13866 14781 15621
Size: 2303 1851 915 840
Fragments arranged by size:
3190 2303 1851 1560 1452 1154 915 905
897 840 311 243
PshAI GACnn'nnGTC
Cuts at: 0 1277 9392 11870 12382 15621
Size: 1277 8115 2478 512 3239
Fragments arranged by size:
8115 3239 2478 1277 512
PsiI TTA'TAA
Cuts at: 0 1364 3328 10195 15621
Size: 1364 1964 6867 5426
Fragments arranged by size:
6867 5426 1964 1364
PspOMI G'GGCC_C
Cuts at: 0 177 8361 8660 15621
Size: 177 8184 299 6961
Fragments arranged by size:
8184 6961 299 177
PspXI vC'TCGA_Gb
Cuts at: 0 5866 13036 15621
Size: 5866 7170 2585
Fragments arranged by size:
7170 5866 2585
PstI C_TGCA'G
Cuts at: 0 55 84 524 773 1625 2569 2629 2664
Size: 55 29 440 249 852 944 60 35
Cuts at: 2664 4256 4843 5032 5477 6397 6481 6968 7371
Size: 1592 587 189 445 920 84 487 403
Cuts at: 7371 8504 8898 9158 9167 9253 9317 9695 10049
Size: 1133 394 260 9 86 64 378 354
Cuts at: 10049 10376 11363 11477 12720 12782 13353 13850 14230
Size: 327 987 114 1243 62 571 497 380
Cuts at: 14230 14318 14569 15047 15621
Size: 88 251 478 574
Fragments arranged by size:
1592 1243 1133 987 944 920 852 587
574 571 497 487 478 445 440 403
394 380 378 354 327 260 251 249
189 114 88 86 84 64 62 60
55 35 29 9
PvuII CAG'CTG
Cuts at: 0 1048 1722 5057 5124 5697 7621 7689 7983
Size: 1048 674 3335 67 573 1924 68 294
Cuts at: 7983 9048 10395 11135 12136 14047 14469 15042 15112
Size: 1065 1347 740 1001 1911 422 573 70
Cuts at: 15112 15135 15267 15566 15589 15621
Size: 23 132 299 23 32
Fragments arranged by size:
3335 1924 1911 1347 1065 1048 1001 740
674 573 573 422 299 294 132 70
68 67 32 23 23
SacI G_AGCT'C
Cuts at: 0 722 3013 3643 4199 5147 5291 6331 7184
Size: 722 2291 630 556 948 144 1040 853
Cuts at: 7184 8729 9572 11684 15621
Size: 1545 843 2112 3937
Fragments arranged by size:
3937 2291 2112 1545 1040 948 853 843
722 630 556 144
SacII CC_GC'GG
Cuts at: 0 2119 2846 3122 15621
Size: 2119 727 276 12499
Fragments arranged by size:
12499 2119 727 276
SalI G'TCGA_C
Cuts at: 0 7087 15621
Size: 7087 8534
SanDI GG'GwC_CC
Cuts at: 0 2714 5904 9401 9712 15621
Size: 2714 3190 3497 311 5909
Fragments arranged by size:
5909 3497 3190 2714 311
SapI GCTCTTCn'nnn_
Cuts at: 0 3709 5279 6336 9547 12523 15621
Size: 3709 1570 1057 3211 2976 3098
Fragments arranged by size:
3709 3211 3098 2976 1570 1057
SbfI CC_TGCA'GG
Cuts at: 0 524 6397 15621
Size: 524 5873 9224
Fragments arranged by size:
9224 5873 524
ScaI AGT'ACT
Cuts at: 0 1483 4738 9355 9859 10066 15621
Size: 1483 3255 4617 504 207 5555
Fragments arranged by size:
5555 4617 3255 1483 504 207
SexAI A'CCwGG_T
Cuts at: 0 1562 2024 2306 4973 8408 12212 12833 12980
Size: 1562 462 282 2667 3435 3804 621 147
Cuts at: 12980 14816 15621
Size: 1836 805
Fragments arranged by size:
3804 3435 2667 1836 1562 805 621 462
282 147
SfcI C'TryA_G
Cuts at: 0 51 80 520 769 821 1621 2565 2625
Size: 51 29 440 249 52 800 944 60
Cuts at: 2625 2660 4252 4839 5028 5473 5673 6393 6477
Size: 35 1592 587 189 445 200 720 84
Cuts at: 6477 6964 7367 7632 8500 8868 8894 9154 9163
Size: 487 403 265 868 368 26 260 9
Cuts at: 9163 9249 9313 9691 10045 10372 11359 11473 11647
Size: 86 64 378 354 327 987 114 174
Cuts at: 11647 12716 12778 13349 13846 14226 14314 14565 15043
Size: 1069 62 571 497 380 88 251 478
Cuts at: 15043 15051 15621
Size: 8 570
Fragments arranged by size:
1592 1069 987 944 868 800 720 587
571 570 497 487 478 445 440 403
380 378 368 354 327 265 260 251
249 200 189 174 114 88 86 84
64 62 60 52 51 35 29 26
9 8
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 431 15621
Size: 431 15190
SfoI GGC'GCC
Cuts at: 0 2079 6111 15621
Size: 2079 4032 9510
Fragments arranged by size:
9510 4032 2079
SmaI CCC'GGG
Cuts at: 0 194 452 759 1124 6821 9718 10264 15621
Size: 194 258 307 365 5697 2897 546 5357
Fragments arranged by size:
5697 5357 2897 546 365 307 258 194
SmlI C'TyrA_G
Cuts at: 0 1015 2404 2584 3805 3961 5223 5866 7120
Size: 1015 1389 180 1221 156 1262 643 1254
Cuts at: 7120 12306 12547 12613 13036 13466 14527 14917 15149
Size: 5186 241 66 423 430 1061 390 232
Cuts at: 15149 15621
Size: 472
Fragments arranged by size:
5186 1389 1262 1254 1221 1061 1015 643
472 430 423 390 241 232 180 156
66
SphI G_CATG'C
Cuts at: 0 10638 12175 13432 15621
Size: 10638 1537 1257 2189
Fragments arranged by size:
10638 2189 1537 1257
SspI AAT'ATT
Cuts at: 0 12457 15621
Size: 12457 3164
StuI AGG'CCT
Cuts at: 0 644 1868 2515 4864 6399 6691 12661 13075
Size: 644 1224 647 2349 1535 292 5970 414
Cuts at: 13075 15196 15621
Size: 2121 425
Fragments arranged by size:
5970 2349 2121 1535 1224 647 644 425
414 292
StyI C'CwwG_G
Cuts at: 0 29 376 950 1995 2709 3112 4067 4916
Size: 29 347 574 1045 714 403 955 849
Cuts at: 4916 5403 6839 8741 8784 9224 10517 10884 11265
Size: 487 1436 1902 43 440 1293 367 381
Cuts at: 11265 11802 12758 12920 13421 14705 15621
Size: 537 956 162 501 1284 916
Fragments arranged by size:
1902 1436 1293 1284 1045 956 955 916
849 714 574 537 501 487 440 403
381 367 347 162 43 29
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1675 1744 4028 4665 5425 6189 6923 8571
Size: 1675 69 2284 637 760 764 734 1648
Cuts at: 8571 8792 9256 9382 9570 11427 11860 12900 12974
Size: 221 464 126 188 1857 433 1040 74
Cuts at: 12974 13727 15006 15157 15621
Size: 753 1279 151 464
Fragments arranged by size:
2284 1857 1675 1648 1279 1040 764 760
753 734 637 464 464 433 221 188
151 126 74 69
TatI w'GTAC_w
Cuts at: 0 1481 1739 4736 9236 9353 9470 9857 10064
Size: 1481 258 2997 4500 117 117 387 207
Cuts at: 10064 11180 12419 12509 13145 14939 15621
Size: 1116 1239 90 636 1794 682
Fragments arranged by size:
4500 2997 1794 1481 1239 1116 682 636
387 258 207 117 117 90
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1124 1156 1915 1947 3625 3657 4512 4544
Size: 1124 32 759 32 1678 32 855 32
Cuts at: 4544 6185 6217 6782 6814 8398 8430 8930 8946
Size: 1641 32 565 32 1584 32 500 16
Cuts at: 8946 8962 8978 9143 9175 9599 9631 10702 10734
Size: 16 16 165 32 424 32 1071 32
Cuts at: 10734 11149 11181 12754 12786 15621
Size: 415 32 1573 32 2835
Fragments arranged by size:
2835 1678 1641 1584 1573 1124 1071 855
759 565 500 424 415 165 32 32
32 32 32 32 32 32 32 32
32 32 16 16 16
Tth111I GACn'n_nGTC
Cuts at: 0 5919 8779 12019 12928 15621
Size: 5919 2860 3240 909 2693
Fragments arranged by size:
5919 3240 2860 2693 909
XbaI T'CTAG_A
Cuts at: 0 14073 15621
Size: 14073 1548
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 468 3658 4960 8340 9144 11214 11551 13591
Size: 468 3190 1302 3380 804 2070 337 2040
Cuts at: 13591 15621
Size: 2030
Fragments arranged by size:
3380 3190 2070 2040 2030 1302 804 468
337
XhoI C'TCGA_G
Cuts at: 0 5866 13036 15621
Size: 5866 7170 2585
Fragments arranged by size:
7170 5866 2585
XmaI C'CCGG_G
Cuts at: 0 192 450 757 1122 6819 9716 10262 15621
Size: 192 258 307 365 5697 2897 546 5359
Fragments arranged by size:
5697 5359 2897 546 365 307 258 192
XmnI GAAnn'nnTTC
Cuts at: 0 1651 2688 7939 8254 8919 11288 11308 12481
Size: 1651 1037 5251 315 665 2369 20 1173
Cuts at: 12481 14389 15518 15621
Size: 1908 1129 103
Fragments arranged by size:
5251 2369 1908 1651 1173 1129 1037 665
315 103 20
ZraI GAC'GTC
Cuts at: 0 6311 7032 9465 9648 15621
Size: 6311 721 2433 183 5973
Fragments arranged by size:
6311 5973 2433 721 183
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AvaI AvrII BaeI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NsiI NspI PasI PciI PflMI PfoI PmlI
PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SmaI SmlI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AscI AseI AsiSI BmtI BsiWI BssHII BstBI FseI
MfeI NaeI NgoMIV NheI NotI NruI PacI PmeI
PsrI PvuI RsrII SgfI SgrAI SnaBI SpeI SrfI
SwaI