Restriction map for
pFN21AE3553
(Linear) (Six-base) MAPSORT of: KIEE3553.seq Check: 1815 from: 1 to: 5898
KIEE3553.orf
With 182 enzymes: SgfI *
July 3, 2012 18:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 372 3770 4854 5898
Size: 372 3398 1084 1044
Fragments arranged by size:
3398 1084 1044 372
AccI GT'mk_AC
Cuts at: 0 971 1292 5714 5898
Size: 971 321 4422 184
Fragments arranged by size:
4422 971 321 184
Acc65I G'GTAC_C
Cuts at: 0 998 4867 5898
Size: 998 3869 1031
Fragments arranged by size:
3869 1031 998
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 263 1333 1591 2125 3933 4468 4622 4976
Size: 263 1070 258 534 1808 535 154 354
Cuts at: 4976 5898
Size: 922
Fragments arranged by size:
1808 1070 922 535 534 354 263 258
154
AflII C'TTAA_G
Cuts at: 0 3987 4590 5898
Size: 3987 603 1308
Fragments arranged by size:
3987 1308 603
AflIII A'CryG_T
Cuts at: 0 974 5333 5898
Size: 974 4359 565
Fragments arranged by size:
4359 974 565
AleI CACnn'nnGTG
Cuts at: 0 580 5898
Size: 580 5318
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 4163 5898
Size: 4163 1735
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 80 112 5898
Size: 80 32 5786
Fragments arranged by size:
5786 80 32
AlwNI CAG_nnn'CTG
Cuts at: 0 105 387 3500 3655 4446 4659 4846 5898
Size: 105 282 3113 155 791 213 187 1052
Fragments arranged by size:
3113 1052 791 282 213 187 155 105
ApaI G_GGCC'C
Cuts at: 0 5841 5898
Size: 5841 57
ApaLI G'TGCA_C
Cuts at: 0 826 1445 2552 5898
Size: 826 619 1107 3346
Fragments arranged by size:
3346 1107 826 619
ApoI r'AATT_y
Cuts at: 0 1234 1435 1874 1887 1938 1957 3140 3973
Size: 1234 201 439 13 51 19 1183 833
Cuts at: 3973 4243 4294 4663 5898
Size: 270 51 369 1235
Fragments arranged by size:
1235 1234 1183 833 439 369 270 201
51 51 19 13
AseI AT'TA_AT
Cuts at: 0 3451 4493 5898
Size: 3451 1042 1405
Fragments arranged by size:
3451 1405 1042
AvaI C'yCGr_G
Cuts at: 0 5129 5898
Size: 5129 769
AvrII C'CTAG_G
Cuts at: 0 487 3162 5898
Size: 487 2675 2736
Fragments arranged by size:
2736 2675 487
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 4756 4789 4859 4892 5402 5435 5898
Size: 4756 33 70 33 510 33 463
Fragments arranged by size:
4756 510 463 70 33 33 33
BamHI G'GATC_C
Cuts at: 0 510 5898
Size: 510 5388
BanI G'GyrC_C
Cuts at: 0 483 998 4867 5631 5643 5732 5898
Size: 483 515 3869 764 12 89 166
Fragments arranged by size:
3869 764 515 483 166 89 12
BanII G_rGCy'C
Cuts at: 0 539 1574 2296 3389 4861 5458 5841 5898
Size: 539 1035 722 1093 1472 597 383 57
Fragments arranged by size:
1472 1093 1035 722 597 539 383 57
BbeI G_GCGC'C
Cuts at: 0 5736 5898
Size: 5736 162
BbsI GAAGACnn'nnnn_
Cuts at: 0 251 524 4456 5898
Size: 251 273 3932 1442
Fragments arranged by size:
3932 1442 273 251
BbvCI CC'TCA_GC
Cuts at: 0 2659 2690 5898
Size: 2659 31 3208
Fragments arranged by size:
3208 2659 31
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2373 2407 5898
Size: 2373 34 3491
Fragments arranged by size:
3491 2373 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1158 1605 1845 1854 2569 4757 5430 5898
Size: 1158 447 240 9 715 2188 673 468
Fragments arranged by size:
2188 1158 715 673 468 447 240 9
BclI T'GATC_A
Cuts at: 0 2543 5898
Size: 2543 3355
BglI GCCn_nnn'nGGC
Cuts at: 0 1681 5436 5898
Size: 1681 3755 462
Fragments arranged by size:
3755 1681 462
BglII A'GATC_T
Cuts at: 0 2028 3005 3237 5898
Size: 2028 977 232 2661
Fragments arranged by size:
2661 2028 977 232
BlpI GC'TnA_GC
Cuts at: 0 2703 5898
Size: 2703 3195
Bme1580I G_kGCm'C
Cuts at: 0 830 1449 2016 2556 5648 5841 5898
Size: 830 619 567 540 3092 193 57
Fragments arranged by size:
3092 830 619 567 540 193 57
BmgBI CAC'GTC
Cuts at: 0 423 5576 5774 5898
Size: 423 5153 198 124
Fragments arranged by size:
5153 423 198 124
BmrI ACTGGGnnnn_n'
Cuts at: 0 294 462 478 2744 3291 5898
Size: 294 168 16 2266 547 2607
Fragments arranged by size:
2607 2266 547 294 168 16
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1380 5060 5511 5711 5898
Size: 1380 3680 451 200 187
Fragments arranged by size:
3680 1380 451 200 187
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 127 673 2279 2635 5322 5465 5848 5898
Size: 127 546 1606 356 2687 143 383 50
Fragments arranged by size:
2687 1606 546 383 356 143 127 50
Bpu10I CC'TnA_GC
Cuts at: 0 2563 2659 2690 3874 4758 5898
Size: 2563 96 31 1184 884 1140
Fragments arranged by size:
2563 1184 1140 884 96 31
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 735 4663 4682 5013 5055 5898
Size: 735 3928 19 331 42 843
Fragments arranged by size:
3928 843 735 331 42 19
BsaI GGTCTCn'nnnn_
Cuts at: 0 4102 4701 5154 5892 5898
Size: 4102 599 453 738 6
Fragments arranged by size:
4102 738 599 453 6
BsaAI yAC'GTr
Cuts at: 0 2055 2557 5898
Size: 2055 502 3341
Fragments arranged by size:
3341 2055 502
BsaBI GATnn'nnATC
Cuts at: 0 3626 5898
Size: 3626 2272
BsaHI Gr'CG_yC
Cuts at: 0 5733 5898
Size: 5733 165
BsaWI w'CCGG_w
Cuts at: 0 410 513 756 2060 4200 5898
Size: 410 103 243 1304 2140 1698
Fragments arranged by size:
2140 1698 1304 410 243 103
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 80 110 2495 2525 3469 3499 3577 3607
Size: 80 30 2385 30 944 30 78 30
Cuts at: 3607 3991 4021 4306 4336 5463 5493 5743 5773
Size: 384 30 285 30 1127 30 250 30
Cuts at: 5773 5898
Size: 125
Fragments arranged by size:
2385 1127 944 384 285 250 125 80
78 30 30 30 30 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 661 2200 3601 3658 4920 5683 5898
Size: 661 1539 1401 57 1262 763 215
Fragments arranged by size:
1539 1401 1262 763 661 215 57
BseYI C'CCAG_C
Cuts at: 0 1164 2008 3230 4905 5898
Size: 1164 844 1222 1675 993
Fragments arranged by size:
1675 1222 1164 993 844
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 604 929 3525 3748 4357 5249 5482 5589
Size: 604 325 2596 223 609 892 233 107
Cuts at: 5589 5898
Size: 309
Fragments arranged by size:
2596 892 609 604 325 309 233 223
107
BsiEI CG_ry'CG
Cuts at: 0 411 846 5898
Size: 411 435 5052
Fragments arranged by size:
5052 435 411
BsiHKAI G_wGCw'C
Cuts at: 0 20 539 830 1449 1574 2045 2296 2556
Size: 20 519 291 619 125 471 251 260
Cuts at: 2556 3173 3389 3761 5898
Size: 617 216 372 2137
Fragments arranged by size:
2137 619 617 519 471 372 291 260
251 216 125 20
BsmI GAATG_Cn'
Cuts at: 0 876 1038 2201 4058 4567 5898
Size: 876 162 1163 1857 509 1331
Fragments arranged by size:
1857 1331 1163 876 509 162
BsmBI CGTCTCn'nnnn_
Cuts at: 0 5222 5898
Size: 5222 676
Bsp1286I G_dGCh'C
Cuts at: 0 20 539 830 1449 1574 2016 2045 2296
Size: 20 519 291 619 125 442 29 251
Cuts at: 2296 2556 3173 3389 3761 4861 5458 5648 5841
Size: 260 617 216 372 1100 597 190 193
Cuts at: 5841 5898
Size: 57
Fragments arranged by size:
1100 619 617 597 519 442 372 291
260 251 216 193 190 125 57 29
20
BspEI T'CCGG_A
Cuts at: 0 513 4200 5898
Size: 513 3687 1698
Fragments arranged by size:
3687 1698 513
BspHI T'CATG_A
Cuts at: 0 353 1566 3251 3267 5898
Size: 353 1213 1685 16 2631
Fragments arranged by size:
2631 1685 1213 353 16
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 372 938 1054 3770 4854 5898
Size: 372 566 116 2716 1084 1044
Fragments arranged by size:
2716 1084 1044 566 372 116
BsrBI CCG'CTC
Cuts at: 0 2079 3420 5721 5898
Size: 2079 1341 2301 177
Fragments arranged by size:
2301 2079 1341 177
BsrDI GCAATG_nn'
Cuts at: 0 465 509 765 2714 5898
Size: 465 44 256 1949 3184
Fragments arranged by size:
3184 1949 465 256 44
BsrFI r'CCGG_y
Cuts at: 0 338 689 5564 5628 5640 5898
Size: 338 351 4875 64 12 258
Fragments arranged by size:
4875 351 338 258 64 12
BssSI C'ACGA_G
Cuts at: 0 19 4823 5898
Size: 19 4804 1075
Fragments arranged by size:
4804 1075 19
BstAPI GCAn_nnn'nTGC
Cuts at: 0 4344 5208 5898
Size: 4344 864 690
Fragments arranged by size:
4344 864 690
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1269 1428 1900 2646 2900 5805 5826 5898
Size: 1269 159 472 746 254 2905 21 72
Fragments arranged by size:
2905 1269 746 472 254 159 72 21
BstYI r'GATC_y
Cuts at: 0 101 510 2028 3005 3237 5898
Size: 101 409 1518 977 232 2661
Fragments arranged by size:
2661 1518 977 409 232 101
BstZ17I GTA'TAC
Cuts at: 0 972 5898
Size: 972 4926
Bsu36I CC'TnA_GG
Cuts at: 0 373 3709 5586 5898
Size: 373 3336 1877 312
Fragments arranged by size:
3336 1877 373 312
BtgI C'CryG_G
Cuts at: 0 2444 5860 5898
Size: 2444 3416 38
Fragments arranged by size:
3416 2444 38
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 906 4062 5898
Size: 906 3156 1836
Fragments arranged by size:
3156 1836 906
BtsI GCAGTG_nn'
Cuts at: 0 801 2434 5175 5253 5550 5898
Size: 801 1633 2741 78 297 348
Fragments arranged by size:
2741 1633 801 348 297 78
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 5171 5206 5898
Size: 5171 35 692
Fragments arranged by size:
5171 692 35
DraI TTT'AAA
Cuts at: 0 1971 3309 5898
Size: 1971 1338 2589
Fragments arranged by size:
2589 1971 1338
DraIII CAC_nnn'GTG
Cuts at: 0 5248 5898
Size: 5248 650
EaeI y'GGCC_r
Cuts at: 0 691 1259 1302 2636 4673 5898
Size: 691 568 43 1334 2037 1225
Fragments arranged by size:
2037 1334 1225 691 568 43
EarI CTCTTCn'nnn_
Cuts at: 0 337 613 1246 1556 2177 3394 5898
Size: 337 276 633 310 621 1217 2504
Fragments arranged by size:
2504 1217 633 621 337 310 276
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 3309 5687 5898
Size: 3309 2378 211
Fragments arranged by size:
3309 2378 211
EcoICRI GAG'CTC
Cuts at: 0 537 1572 2294 3387 5898
Size: 537 1035 722 1093 2511
Fragments arranged by size:
2511 1093 1035 722 537
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 127 263 673 1333 1591 2125 2279 2635
Size: 127 136 410 660 258 534 154 356
Cuts at: 2635 3933 4468 4622 4976 5322 5465 5848 5898
Size: 1298 535 154 354 346 143 383 50
Fragments arranged by size:
1298 660 535 534 410 383 356 354
346 258 154 154 143 136 127 50
EcoNI CCTnn'n_nnAGG
Cuts at: 0 643 5898
Size: 643 5255
EcoO109I rG'GnC_Cy
Cuts at: 0 2538 4179 5234 5837 5870 5898
Size: 2538 1641 1055 603 33 28
Fragments arranged by size:
2538 1641 1055 603 33 28
EcoRI G'AATT_C
Cuts at: 0 4294 5898
Size: 4294 1604
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1713 2997 4773 5898
Size: 1713 1284 1776 1125
Fragments arranged by size:
1776 1713 1284 1125
FspI TGC'GCA
Cuts at: 0 5680 5898
Size: 5680 218
HaeII r_GCGC'y
Cuts at: 0 432 5736 5898
Size: 432 5304 162
Fragments arranged by size:
5304 432 162
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 540 572 1309 1341 2018 2050 4306 4338
Size: 540 32 737 32 677 32 2256 32
Cuts at: 4338 5541 5573 5898
Size: 1203 32 325
Fragments arranged by size:
2256 1203 737 677 540 325 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 4731 4914 5898
Size: 4731 183 984
Fragments arranged by size:
4731 984 183
HindIII A'AGCT_T
Cuts at: 0 3175 4215 4946 5031 5898
Size: 3175 1040 731 85 867
Fragments arranged by size:
3175 1040 867 731 85
KasI G'GCGC_C
Cuts at: 0 5732 5898
Size: 5732 166
KpnI G_GTAC'C
Cuts at: 0 1002 4871 5898
Size: 1002 3869 1027
Fragments arranged by size:
3869 1027 1002
MfeI C'AATT_G
Cuts at: 0 4415 5898
Size: 4415 1483
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 659 3044 3936 4068 5206 5318 5537 5558
Size: 659 2385 892 132 1138 112 219 21
Cuts at: 5558 5756 5898
Size: 198 142
Fragments arranged by size:
2385 1138 892 659 219 198 142 132
112 21
MscI TGG'CCA
Cuts at: 0 1261 1304 2638 5898
Size: 1261 43 1334 3260
Fragments arranged by size:
3260 1334 1261 43
MslI CAynn'nnrTG
Cuts at: 0 262 580 1426 1586 2038 2399 3508 4081
Size: 262 318 846 160 452 361 1109 573
Cuts at: 4081 5898
Size: 1817
Fragments arranged by size:
1817 1109 846 573 452 361 318 262
160
MspA1I CmG'CkG
Cuts at: 0 2195 4446 4909 5300 5862 5898
Size: 2195 2251 463 391 562 36
Fragments arranged by size:
2251 2195 562 463 391 36
NarI GG'CG_CC
Cuts at: 0 5733 5898
Size: 5733 165
NdeI CA'TA_TG
Cuts at: 0 66 5898
Size: 66 5832
NspI r_CATG'y
Cuts at: 0 978 2398 3120 3153 5010 5337 5898
Size: 978 1420 722 33 1857 327 561
Fragments arranged by size:
1857 1420 978 722 561 327 33
PasI CC'CwG_GG
Cuts at: 0 2370 5898
Size: 2370 3528
PciI A'CATG_T
Cuts at: 0 974 5333 5898
Size: 974 4359 565
Fragments arranged by size:
4359 974 565
PfoI T'CCnGG_A
Cuts at: 0 104 365 2093 2339 2360 2723 5898
Size: 104 261 1728 246 21 363 3175
Fragments arranged by size:
3175 1728 363 261 246 104 21
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 44 76 80 112 4181 4213 5898
Size: 44 32 4 32 4069 32 1685
Fragments arranged by size:
4069 1685 44 32 32 32 4
PpuMI rG'GwC_Cy
Cuts at: 0 2538 4179 5898
Size: 2538 1641 1719
Fragments arranged by size:
2538 1719 1641
PsiI TTA'TAA
Cuts at: 0 1043 3002 3043 5898
Size: 1043 1959 41 2855
Fragments arranged by size:
2855 1959 1043 41
PspOMI G'GGCC_C
Cuts at: 0 5837 5898
Size: 5837 61
PstI C_TGCA'G
Cuts at: 0 1850 2414 2429 2849 3062 4187 4536 5898
Size: 1850 564 15 420 213 1125 349 1362
Fragments arranged by size:
1850 1362 1125 564 420 349 213 15
PvuII CAG'CTG
Cuts at: 0 4446 4909 5300 5898
Size: 4446 463 391 598
Fragments arranged by size:
4446 598 463 391
SacI G_AGCT'C
Cuts at: 0 539 1574 2296 3389 5898
Size: 539 1035 722 1093 2509
Fragments arranged by size:
2509 1093 1035 722 539
SacII CC_GC'GG
Cuts at: 0 5863 5898
Size: 5863 35
SapI GCTCTTCn'nnn_
Cuts at: 0 613 1246 3394 5898
Size: 613 633 2148 2504
Fragments arranged by size:
2504 2148 633 613
SbfI CC_TGCA'GG
Cuts at: 0 2414 5898
Size: 2414 3484
SexAI A'CCwGG_T
Cuts at: 0 479 5898
Size: 479 5419
SfcI C'TryA_G
Cuts at: 0 876 1846 2410 2425 2845 3058 3864 4183
Size: 876 970 564 15 420 213 806 319
Cuts at: 4183 4532 5538 5898
Size: 349 1006 360
Fragments arranged by size:
1006 970 876 806 564 420 360 349
319 213 15
SfoI GGC'GCC
Cuts at: 0 5734 5898
Size: 5734 164
SmlI C'TyrA_G
Cuts at: 0 714 3987 4590 4642 4697 5028 5034 5898
Size: 714 3273 603 52 55 331 6 864
Fragments arranged by size:
3273 864 714 603 331 55 52 6
SspI AAT'ATT
Cuts at: 0 1123 1179 2870 4491 4968 5898
Size: 1123 56 1691 1621 477 930
Fragments arranged by size:
1691 1621 1123 930 477 56
StyI C'CwwG_G
Cuts at: 0 487 1643 1656 3162 5898
Size: 487 1156 13 1506 2736
Fragments arranged by size:
2736 1506 1156 487 13
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 418 832 1793 4271 4299 5898
Size: 418 414 961 2478 28 1599
Fragments arranged by size:
2478 1599 961 418 414 28
TatI w'GTAC_w
Cuts at: 0 2309 5898
Size: 2309 3589
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3991 4023 5110 5142 5463 5495 5743 5775
Size: 3991 32 1087 32 321 32 248 32
Cuts at: 5775 5898
Size: 123
Fragments arranged by size:
3991 1087 321 248 123 32 32 32
32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2394 2731 4810 5830 5898
Size: 2394 337 2079 1020 68
Fragments arranged by size:
2394 2079 1020 337 68
XmnI GAAnn'nnTTC
Cuts at: 0 4063 4294 5898
Size: 4063 231 1604
Fragments arranged by size:
4063 1604 231
Enzymes that do cut:
AarI AccI Acc65I AcuI AflII AflIII AleI AlfI
AloI AlwNI ApaI ApaLI ApoI AseI AvaI AvrII
BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstXI BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI CspCI DraI DraIII EaeI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MfeI MmeI MscI MslI
MspA1I NarI NdeI NspI PasI PciI PfoI PpiI
PpuMI PsiI PspOMI PstI PvuII SacI SacII SapI
SbfI SexAI SfcI SfoI SmlI SspI StyI TaqII
TatI TstI XcmI XmnI
Enzymes that do not cut:
AatII AclI AfeI AgeI AhdI AscI AsiSI BfrBI
BmtI BsiWI BsrGI BssHII BstBI BstEII ClaI DrdI
EagI EcoRV FseI FspAI HpaI MluI NaeI NcoI
NgoMIV NheI NotI NruI NsiI PacI PflMI PmeI
PmlI PshAI PspXI PsrI PvuI RsrII SalI SanDI
ScaI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI StuI SwaI Tth111I XbaI XhoI XmaI ZraI